(Circular) (Six-base) MAPSORT of: pFN21AB6249.seq Check: 4523 from: 1 to: 6973 pFN21AB6249 6973 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 4316 1937 Size: 676 1703 4594 Fragments arranged by size: 4594 1703 676 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3344 3773 3998 4155 5036 6420 6688 1632 Size: 1712 429 225 157 881 1384 268 1917 Fragments arranged by size: 1917 1712 1384 881 429 268 225 157 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 2036 5872 6849 1203 Size: 833 3836 977 1327 Fragments arranged by size: 3836 1327 977 833 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3359 1184 Size: 2175 4798 AlwNI CAG_nnn'CTG Cuts at: 2569 3061 3322 4716 6288 2569 Size: 492 261 1394 1572 3254 Fragments arranged by size: 3254 1572 1394 492 261 ApaI G_GGCC'C Cuts at: 2892 2892 Size: 6973 ApaLI G'TGCA_C Cuts at: 5030 6186 5030 Size: 1156 5817 ApoI r'AATT_y Cuts at: 1493 3412 4282 4508 4660 6769 1493 Size: 1919 870 226 152 2109 1697 Fragments arranged by size: 2109 1919 1697 870 226 152 AseI AT'TA_AT Cuts at: 160 1032 5527 160 Size: 872 4495 1606 Fragments arranged by size: 4495 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 2232 3738 4298 1870 Size: 73 289 1506 560 4545 Fragments arranged by size: 4545 1506 560 289 73 AvrII C'CTAG_G Cuts at: 2503 2503 Size: 6973 BamHI G'GATC_C Cuts at: 2875 4303 4670 2875 Size: 1428 367 5178 Fragments arranged by size: 5178 1428 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2178 2263 2363 2630 2853 Size: 325 217 627 391 85 100 267 223 Cuts at: 2853 2963 4294 5749 618 Size: 110 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 391 325 267 223 217 110 100 85 BanII G_rGCy'C Cuts at: 1391 2154 2892 3255 3573 3656 3966 4292 1391 Size: 763 738 363 318 83 310 326 4072 Fragments arranged by size: 4072 763 738 363 326 318 310 83 BbeI G_GCGC'C Cuts at: 2267 2267 Size: 6973 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2751 3932 928 Size: 971 852 1181 3969 Fragments arranged by size: 3969 1181 971 852 BbvCI CC'TCA_GC Cuts at: 3281 3281 Size: 6973 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2530 2564 3083 3117 5164 5198 1903 Size: 34 593 34 519 34 2047 34 3678 Fragments arranged by size: 3678 2047 593 519 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4865 6075 3251 Size: 1614 1210 4149 Fragments arranged by size: 4149 1614 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3660 5584 136 Size: 107 122 71 631 605 1988 1924 1525 Fragments arranged by size: 1988 1924 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2311 3204 6968 2311 Size: 893 3764 2316 Fragments arranged by size: 3764 2316 893 BlpI GC'TnA_GC Cuts at: 2153 2814 4390 2153 Size: 661 1576 4736 Fragments arranged by size: 4736 1576 661 Bme1580I G_kGCm'C Cuts at: 1790 2873 2892 2966 5034 6190 1790 Size: 1083 19 74 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 1083 74 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4704 5662 442 Size: 416 53 83 399 24 3287 958 1753 Fragments arranged by size: 3287 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2330 4285 2330 Size: 1955 5018 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2606 2641 2843 3506 3565 4240 4280 Size: 965 402 35 202 663 59 675 40 Cuts at: 4280 5633 1239 Size: 1353 2579 Fragments arranged by size: 2579 1353 965 675 663 402 202 59 40 35 Bpu10I CC'TnA_GC Cuts at: 3281 4101 3281 Size: 820 6153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3425 5098 5963 6261 3425 Size: 1673 865 298 4137 Fragments arranged by size: 4137 1673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3735 5636 882 Size: 617 2236 1901 2219 Fragments arranged by size: 2236 2219 1901 617 BsaAI yAC'GTr Cuts at: 493 1204 2883 4764 493 Size: 711 1679 1881 2702 Fragments arranged by size: 2702 1881 1679 711 BsaBI GATnn'nnATC Cuts at: 2129 2192 6967 2129 Size: 63 4775 2135 Fragments arranged by size: 4775 2135 63 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2264 4780 5162 5797 Size: 53 83 186 936 731 2516 382 635 Cuts at: 5797 275 Size: 1451 Fragments arranged by size: 2516 1451 936 731 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 5406 5804 6078 6225 6636 2316 Size: 3090 398 274 147 411 2653 Fragments arranged by size: 3090 2653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6748 Fragments arranged by size: 6748 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2322 3062 3368 3664 3710 1186 Size: 1136 740 306 296 46 4449 Fragments arranged by size: 4449 1136 740 306 296 46 BseYI C'CCAG_C Cuts at: 6176 6672 6176 Size: 496 6477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2711 4135 1163 Size: 573 975 1424 4001 Fragments arranged by size: 4001 1424 975 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 3573 3966 4251 5034 5119 6190 2154 Size: 1419 393 285 783 85 1071 2937 Fragments arranged by size: 2937 1419 1071 783 393 285 85 BsmI GAATG_Cn' Cuts at: 2698 4039 4484 4577 2698 Size: 1341 445 93 5094 Fragments arranged by size: 5094 1341 445 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2873 2892 2966 3255 3573 3656 Size: 399 364 719 19 74 289 318 83 Cuts at: 3656 3966 4251 4292 5034 5119 6190 1391 Size: 310 285 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 399 364 318 310 289 285 85 83 74 41 19 BspEI T'CCGG_A Cuts at: 2316 6636 2316 Size: 4320 2653 BspHI T'CATG_A Cuts at: 2196 2413 4862 6592 2196 Size: 217 2449 1730 2577 Fragments arranged by size: 2577 2449 1730 217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 4328 844 Size: 1439 2045 3489 Fragments arranged by size: 3489 2045 1439 BsrBI CCG'CTC Cuts at: 1394 1823 2173 4860 1394 Size: 429 350 2687 3507 Fragments arranged by size: 3507 2687 429 350 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 5468 5642 65 Size: 1183 1140 3080 174 1396 Fragments arranged by size: 3080 1396 1183 1140 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2142 3339 5617 5804 1700 Size: 110 332 1197 2278 187 2869 Fragments arranged by size: 2869 2278 1197 332 187 110 BsrGI T'GTAC_A Cuts at: 96 3714 96 Size: 3618 3355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 5033 6045 5033 Size: 1012 5961 BstAPI GCAn_nnn'nTGC Cuts at: 794 2538 2689 2842 794 Size: 1744 151 153 4925 Fragments arranged by size: 4925 1744 153 151 BstBI TT'CG_AA Cuts at: 5777 5777 Size: 6973 BstEII G'GTnAC_C Cuts at: 1184 2013 1184 Size: 829 6144 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2521 2224 Size: 297 6676 BstYI r'GATC_y Cuts at: 1972 2187 2311 2437 2875 3157 3204 4303 4670 Size: 215 124 126 438 282 47 1099 367 Cuts at: 4670 5055 5072 6524 6610 6622 6968 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1099 438 385 367 346 282 215 126 124 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2244 2244 Size: 6973 BtgI C'CryG_G Cuts at: 513 1065 2289 3212 3417 3657 4000 4080 513 Size: 552 1224 923 205 240 343 80 3406 Fragments arranged by size: 3406 1224 923 552 343 240 205 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6234 Fragments arranged by size: 6234 683 56 BtsI GCAGTG_nn' Cuts at: 2144 2699 4489 5283 5303 2144 Size: 555 1790 794 20 3814 Fragments arranged by size: 3814 1790 794 555 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3982 4017 529 Size: 35 3418 35 3485 Fragments arranged by size: 3485 3418 35 35 DraI TTT'AAA Cuts at: 4277 4277 Size: 6973 DraIII CAC_nnn'GTG Cuts at: 3111 3111 Size: 6973 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 4420 5309 Size: 54 1425 50 275 18 456 2134 889 Cuts at: 5309 6938 8 Size: 1629 43 Fragments arranged by size: 2134 1629 1425 889 456 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2711 3888 4109 4239 4903 1353 Size: 1358 1177 221 130 664 3423 Fragments arranged by size: 3423 1358 1177 664 221 130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5560 5934 6080 387 Size: 1064 138 3971 374 146 1280 Fragments arranged by size: 3971 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 3571 3964 2152 Size: 1419 393 5161 Fragments arranged by size: 5161 1419 393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2606 2641 2843 3344 3506 3565 Size: 393 572 402 35 202 501 162 59 Cuts at: 3565 3773 3998 4155 4240 4280 5036 5633 6420 Size: 208 225 157 85 40 756 597 787 Cuts at: 6420 6688 1239 Size: 268 1524 Fragments arranged by size: 1524 787 756 597 572 501 402 393 268 225 208 202 162 157 85 59 40 35 EcoNI CCTnn'n_nnAGG Cuts at: 4262 4262 Size: 6973 EcoO109I rG'GnC_Cy Cuts at: 2043 2556 2908 2043 Size: 513 352 6108 Fragments arranged by size: 6108 513 352 EcoRI G'AATT_C Cuts at: 3412 4282 4660 3412 Size: 870 378 5725 Fragments arranged by size: 5725 870 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2081 2081 Size: 6973 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 2065 2267 3872 6120 1678 Size: 387 202 1605 2248 2531 Fragments arranged by size: 2531 2248 1605 387 202 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 3604 3636 5614 Size: 32 135 32 806 32 1194 32 1978 Cuts at: 5614 5646 5688 5720 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 1194 806 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2614 4317 4563 669 Size: 988 281 676 1703 246 3079 Fragments arranged by size: 3079 1703 988 676 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6973 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KasI G'GCGC_C Cuts at: 2263 2263 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 4572 4572 Size: 6973 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3049 3432 4113 6087 6271 1305 Size: 1744 383 681 1974 184 2007 Fragments arranged by size: 2007 1974 1744 681 383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 10 Size: 54 1425 343 456 4695 Fragments arranged by size: 4695 1425 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 4931 5290 5449 518 Size: 648 1051 2714 359 159 2042 Fragments arranged by size: 2714 2042 1051 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3237 3280 3393 4063 4254 4389 5066 6214 Size: 1631 43 113 670 191 135 677 1148 Cuts at: 6214 6459 1606 Size: 245 2120 Fragments arranged by size: 2120 1631 1148 677 670 245 191 135 113 43 NaeI GCC'GGC Cuts at: 2144 2144 Size: 6973 NarI GG'CG_CC Cuts at: 2264 2264 Size: 6973 NcoI C'CATG_G Cuts at: 513 1065 2289 3417 3657 513 Size: 552 1224 1128 240 3829 Fragments arranged by size: 3829 1224 1128 552 240 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NgoMIV G'CCGG_C Cuts at: 2142 2142 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 2040 4331 5876 1132 Size: 97 811 2291 1545 2229 Fragments arranged by size: 2291 2229 1545 811 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 2036 5872 2036 Size: 3836 3137 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3148 1108 Size: 902 1138 4933 Fragments arranged by size: 4933 1138 902 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2556 2556 Size: 6973 PshAI GACnn'nnGTC Cuts at: 1613 3932 1613 Size: 2319 4654 PsiI TTA'TAA Cuts at: 4543 4543 Size: 6973 PspOMI G'GGCC_C Cuts at: 2888 2888 Size: 6973 PstI C_TGCA'G Cuts at: 830 2466 2565 3006 3069 3348 3627 3945 4325 Size: 1636 99 441 63 279 279 318 380 Cuts at: 4325 830 Size: 3478 Fragments arranged by size: 3478 1636 441 380 318 279 279 99 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 3237 3280 3393 4063 4254 3237 Size: 43 113 670 191 5956 Fragments arranged by size: 5956 670 191 113 43 SacI G_AGCT'C Cuts at: 2154 3573 3966 2154 Size: 1419 393 5161 Fragments arranged by size: 5161 1419 393 SalI G'TCGA_C Cuts at: 1936 2612 4315 1936 Size: 676 1703 4594 Fragments arranged by size: 4594 1703 676 SanDI GG'GwC_CC Cuts at: 2556 2556 Size: 6973 SapI GCTCTTCn'nnn_ Cuts at: 4239 4239 Size: 6973 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 2297 5221 6933 2297 Size: 2924 1712 2337 Fragments arranged by size: 2924 2337 1712 SfcI C'TryA_G Cuts at: 826 1045 2462 2561 3002 3065 3344 3623 3941 Size: 219 1417 99 441 63 279 279 318 Cuts at: 3941 4321 5456 6137 6328 826 Size: 380 1135 681 191 1471 Fragments arranged by size: 1471 1417 1135 681 441 380 318 279 279 219 191 99 63 SfoI GGC'GCC Cuts at: 2265 2265 Size: 6973 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 4300 1872 Size: 2428 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3440 3738 5077 5978 6240 820 Size: 197 926 1497 298 1339 901 262 1553 Fragments arranged by size: 1553 1497 1339 926 901 298 262 197 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6973 SphI G_CATG'C Cuts at: 1132 4331 1132 Size: 3199 3774 SspI AAT'ATT Cuts at: 5 52 4897 5 Size: 47 4845 2081 Fragments arranged by size: 4845 2081 47 StuI AGG'CCT Cuts at: 1517 4268 1517 Size: 2751 4222 StyI C'CwwG_G Cuts at: 513 1065 2065 2289 2302 2503 2514 3417 3657 Size: 552 1000 224 13 201 11 903 240 Cuts at: 3657 3885 4412 513 Size: 228 527 3074 Fragments arranged by size: 3074 1000 903 552 527 240 228 224 201 13 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3644 5000 5017 5170 5355 5800 325 Size: 1255 2064 1356 17 153 185 445 1498 Fragments arranged by size: 2064 1498 1356 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 2598 3492 Size: 274 80 33 51 1443 318 303 894 Cuts at: 3492 3714 5219 6931 96 Size: 222 1505 1712 138 Fragments arranged by size: 1712 1505 1443 894 318 303 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6933 6965 6933 Size: 32 6941 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6973 XbaI T'CTAG_A Cuts at: 4309 4309 Size: 6973 XhoI C'TCGA_G Cuts at: 1943 3738 1943 Size: 1795 5178 XmaI C'CCGG_G Cuts at: 1870 4298 1870 Size: 2428 4545 XmnI GAAnn'nnTTC Cuts at: 5102 5102 Size: 6973 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI