Restriction map for
pFN21AB6236
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI ApaI AseI AsnI
Asp718I AvaI AvrII BamHI BbrPI
BcgI BclI BfaI BfrI BlnI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssSI Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV FbaI FseI HpaI KpnI
MaeI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SapI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 168 GTCGAC GT^[AC][GT]AC
AcsI 2005 AAATTC [GA]^AATT[CT]
AhdI 1588 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 328 CCTCAGG CC^T[ACGT]AGG
Aor51HI 278 AGCGCT AGC^GCT
ApoI 2005 AAATTC [GA]^AATT[CT]
AscI 1923 GGCGCGCC GG^CGCGCC
Asp700I 1491 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1588 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 529 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3 GGCGCC GGCGC^C
BbvCI 862 GCTGAGG CCTCAGC(-5/-2)
BciVI 153 GTATCC GTATCC(6/5)
BglI 1126 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1007 AGATCT A^GATCT
BlpI 105 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 105 GCTCAGC GC^T[ACGT]AGC
BsaWI 958 ACCGGA [AT]^CCGG[AT]
BsmAI 1883 GAGAC GTCTC(1/5)
BsmI 1975 GCATTC GAATGC(1/-1)
BspMI 320 ACCTGC ACCTGC(4/8)
BsrDI 570 GCAATG GCAATG(2/0)
BssHII 1924 GCGCGC G^CGCGC
BstEII 249 GGTGACC G^GT[ACGT]ACC
BstPI 249 GGTGACC G^GT[ACGT]ACC
Bsu36I 328 CCTCAGG CC^T[ACGT]AGG
CelII 105 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1588 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1666 CTCTTC CTCTTC(1/4)
Eco47III 278 AGCGCT AGC^GCT
Eco81I 328 CCTCAGG CC^T[ACGT]AGG
EcoNI 1137 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 249 GGTGACC G^GT[ACGT]ACC
EcoT22I 597 ATGCAT ATGCA^T
HincII 168 GTCGAC GT[CT]^[GA]AC
HindII 168 GTCGAC GT[CT]^[GA]AC
HindIII 853 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
Ksp632I 1666 CTCTTC CTCTTC(1/4)
MseI 775 TTAA T^TAA
NarI 3 GGCGCC GG^CGCC
NsiI 597 ATGCAT ATGCA^T
PflFI 529 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1814 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 168 GTCGAC G^TCGAC
SbfI 48 CCTGCAGG CCTGCA^GG
ScaI 1118 AGTACT AGT^ACT
SfoI 3 GGCGCC GGC^GCC
SmlI 1138 CTCAAG C^T[CT][GA]AG
Sse8387I 48 CCTGCAGG CCTGCA^GG
StuI 866 AGGCCT AGG^CCT
TfiI 1218 GAATC G^A[AT]TC
Tru9I 775 TTAA T^TAA
Tsp509I 2006 AATT ^AATT
Tth111I 529 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1814 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1724 CCAAGGACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1491 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 3 GGCGCC G[GA]^CG[CT]C
178 GACGCC
Alw44I 802 GTGCAC G^TGCAC
1176 GTGCAC
AlwI 1554 GATCC GGATC(4/5)
1709 GGATC
ApaLI 802 GTGCAC G^TGCAC
1176 GTGCAC
AviII 14 TGCGCA TGC^GCA
392 TGCGCA
BanII 42 GGGCTC G[GA]GC[CT]^C
717 GAGCCC
BbsI 784 GAAGAC GAAGAC(2/6)
954 GAAGAC
BpmI 364 CTGGAG CTGGAG(16/14)
1735 CTGGAG
BpuAI 784 GAAGAC GAAGAC(2/6)
954 GAAGAC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
178 GACGCC
BsgI 1242 CTGCAC GTGCAG(16/14)
133 GTGCAG
BstAPI 1022 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1517 GCACCTTCTGC
BstXI 1676 CCATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1763 CCAAGAGGCTGG
BstYI 1007 AGATCT [GA]^GATC[CT]
1553 AGATCC
FspI 14 TGCGCA TGC^GCA
392 TGCGCA
HgaI 178 GACGC GACGC(5/10)
200 GCGTC
Hin1I 3 GGCGCC G[GA]^CG[CT]C
178 GACGCC
KspI 432 CCGCGG CCGC^GG
632 CCGCGG
MflI 1007 AGATCT [GA]^GATC[CT]
1553 AGATCC
NspI 683 ACATGC [GA]CATG^[CT]
977 ACATGC
SacII 432 CCGCGG CCGC^GG
632 CCGCGG
XhoII 1007 AGATCT [GA]^GATC[CT]
1553 AGATCC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BalI BanI BbvI
BcnI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I