Restriction map for
pFN21AB6236
(Linear) (Six-base) MAPSORT of: KIBB6236.seq Check: 7271 from: 1 to: 2028
KIBB6236.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 169 2028
Size: 169 1859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1588 1842 2028
Size: 1123 465 254 186
Fragments arranged by size:
1123 465 254 186
AfeI AGC'GCT
Cuts at: 0 280 2028
Size: 280 1748
AhdI GACnn_n'nnGTC
Cuts at: 0 1593 2028
Size: 1593 435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 817 2028
Size: 817 1211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1252 1284 2028
Size: 1252 32 744
Fragments arranged by size:
1252 744 32
AlwNI CAG_nnn'CTG
Cuts at: 0 244 925 1566 2028
Size: 244 681 641 462
Fragments arranged by size:
681 641 462 244
ApaLI G'TGCA_C
Cuts at: 0 802 1176 2028
Size: 802 374 852
Fragments arranged by size:
852 802 374
ApoI r'AATT_y
Cuts at: 0 2005 2028
Size: 2005 23
AscI GG'CGCG_CC
Cuts at: 0 1924 2028
Size: 1924 104
BanI G'GyrC_C
Cuts at: 0 3 628 1528 2028
Size: 3 625 900 500
Fragments arranged by size:
900 625 500 3
BanII G_rGCy'C
Cuts at: 0 46 721 2028
Size: 46 675 1307
Fragments arranged by size:
1307 675 46
BbeI G_GCGC'C
Cuts at: 0 7 2028
Size: 7 2021
BbsI GAAGACnn'nnnn_
Cuts at: 0 791 961 2028
Size: 791 170 1067
Fragments arranged by size:
1067 791 170
BbvCI CC'TCA_GC
Cuts at: 0 863 2028
Size: 863 1165
BciVI GTATCCnnnnn_n'
Cuts at: 0 164 2028
Size: 164 1864
BfrBI ATG'CAT
Cuts at: 0 599 2028
Size: 599 1429
BglI GCCn_nnn'nGGC
Cuts at: 0 1132 2028
Size: 1132 896
BglII A'GATC_T
Cuts at: 0 1007 2028
Size: 1007 1021
BlpI GC'TnA_GC
Cuts at: 0 106 2028
Size: 106 1922
Bme1580I G_kGCm'C
Cuts at: 0 806 1180 2028
Size: 806 374 848
Fragments arranged by size:
848 806 374
BmgBI CAC'GTC
Cuts at: 0 372 1170 2028
Size: 372 798 858
Fragments arranged by size:
858 798 372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 2028
Size: 60 1968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1756 2028
Size: 385 1371 272
Fragments arranged by size:
1371 385 272
Bpu10I CC'TnA_GC
Cuts at: 0 863 2028
Size: 863 1165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 2028
Size: 1123 905
BsaHI Gr'CG_yC
Cuts at: 0 4 179 2028
Size: 4 175 1849
Fragments arranged by size:
1849 175 4
BsaWI w'CCGG_w
Cuts at: 0 958 2028
Size: 958 1070
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 898 928 2028
Size: 226 30 642 30 1100
Fragments arranged by size:
1100 642 226 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 906 1127 2028
Size: 247 659 221 901
Fragments arranged by size:
901 659 247 221
BseYI C'CCAG_C
Cuts at: 0 39 967 1770 2028
Size: 39 928 803 258
Fragments arranged by size:
928 803 258 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1227 2028
Size: 154 1073 801
Fragments arranged by size:
1073 801 154
BsiHKAI G_wGCw'C
Cuts at: 0 806 1180 2015 2028
Size: 806 374 835 13
Fragments arranged by size:
835 806 374 13
BsmI GAATG_Cn'
Cuts at: 0 1975 2028
Size: 1975 53
Bsp1286I G_dGCh'C
Cuts at: 0 46 721 806 1180 2015 2028
Size: 46 675 85 374 835 13
Fragments arranged by size:
835 675 374 85 46 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 2028
Size: 329 1699
BsrDI GCAATG_nn'
Cuts at: 0 577 2028
Size: 577 1451
BssHII G'CGCG_C
Cuts at: 0 1924 2028
Size: 1924 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1028 1523 2028
Size: 1028 495 505
Fragments arranged by size:
1028 505 495
BstEII G'GTnAC_C
Cuts at: 0 249 2028
Size: 249 1779
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1683 1770 2028
Size: 1683 87 258
Fragments arranged by size:
1683 258 87
BstYI r'GATC_y
Cuts at: 0 1007 1553 2028
Size: 1007 546 475
Fragments arranged by size:
1007 546 475
Bsu36I CC'TnA_GG
Cuts at: 0 329 2028
Size: 329 1699
BtgI C'CryG_G
Cuts at: 0 254 432 632 1181 1644 1961 2028
Size: 254 178 200 549 463 317 67
Fragments arranged by size:
549 463 317 254 200 178 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3 2028
Size: 3 2025
BtsI GCAGTG_nn'
Cuts at: 0 402 735 2028
Size: 402 333 1293
Fragments arranged by size:
1293 402 333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 477 512 2028
Size: 477 35 1516
Fragments arranged by size:
1516 477 35
EaeI y'GGCC_r
Cuts at: 0 272 635 827 1452 1629 2028
Size: 272 363 192 625 177 399
Fragments arranged by size:
625 399 363 272 192 177
EarI CTCTTCn'nnn_
Cuts at: 0 1672 2028
Size: 1672 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1123 1588 1756 1842 2028
Size: 385 738 465 168 86 186
Fragments arranged by size:
738 465 385 186 168 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1141 2028
Size: 1141 887
EcoO109I rG'GnC_Cy
Cuts at: 0 540 995 1059 1134 1299 1416 1563 1605
Size: 540 455 64 75 165 117 147 42
Cuts at: 1605 2028
Size: 423
Fragments arranged by size:
540 455 423 165 147 117 75 64
42
FspI TGC'GCA
Cuts at: 0 16 394 2028
Size: 16 378 1634
Fragments arranged by size:
1634 378 16
HaeII r_GCGC'y
Cuts at: 0 7 106 282 2028
Size: 7 99 176 1746
Fragments arranged by size:
1746 176 99 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 151 170 183 226 258 898 930
Size: 138 13 19 13 43 32 640 32
Cuts at: 930 1579 1611 2028
Size: 649 32 417
Fragments arranged by size:
649 640 417 138 43 32 32 32
19 13 13
HincII GTy'rAC
Cuts at: 0 170 2028
Size: 170 1858
HindIII A'AGCT_T
Cuts at: 0 853 2028
Size: 853 1175
KasI G'GCGC_C
Cuts at: 0 3 2028
Size: 3 2025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1826 2028
Size: 1421 405 202
Fragments arranged by size:
1421 405 202
MscI TGG'CCA
Cuts at: 0 829 1454 1631 2028
Size: 829 625 177 397
Fragments arranged by size:
829 625 397 177
MslI CAynn'nnrTG
Cuts at: 0 188 682 799 1649 1681 2028
Size: 188 494 117 850 32 347
Fragments arranged by size:
850 494 347 188 117 32
MspA1I CmG'CkG
Cuts at: 0 434 634 1363 1706 2028
Size: 434 200 729 343 322
Fragments arranged by size:
729 434 343 322 200
NarI GG'CG_CC
Cuts at: 0 4 2028
Size: 4 2024
NcoI C'CATG_G
Cuts at: 0 1181 1644 1961 2028
Size: 1181 463 317 67
Fragments arranged by size:
1181 463 317 67
NsiI A_TGCA'T
Cuts at: 0 601 2028
Size: 601 1427
NspI r_CATG'y
Cuts at: 0 687 981 2028
Size: 687 294 1047
Fragments arranged by size:
1047 687 294
PflMI CCAn_nnn'nTGG
Cuts at: 0 1820 2028
Size: 1820 208
PfoI T'CCnGG_A
Cuts at: 0 362 895 1316 1733 2018 2028
Size: 362 533 421 417 285 10
Fragments arranged by size:
533 421 417 362 285 10
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 2028
Size: 397 32 1599
Fragments arranged by size:
1599 397 32
PpuMI rG'GwC_Cy
Cuts at: 0 995 1059 1299 1416 1605 2028
Size: 995 64 240 117 189 423
Fragments arranged by size:
995 423 240 189 117 64
PstI C_TGCA'G
Cuts at: 0 53 326 1385 2028
Size: 53 273 1059 643
Fragments arranged by size:
1059 643 273 53
SacII CC_GC'GG
Cuts at: 0 435 635 2028
Size: 435 200 1393
Fragments arranged by size:
1393 435 200
SalI G'TCGA_C
Cuts at: 0 168 2028
Size: 168 1860
SbfI CC_TGCA'GG
Cuts at: 0 53 2028
Size: 53 1975
ScaI AGT'ACT
Cuts at: 0 1120 2028
Size: 1120 908
SfcI C'TryA_G
Cuts at: 0 49 322 1381 1558 1887 1935 2028
Size: 49 273 1059 177 329 48 93
Fragments arranged by size:
1059 329 273 177 93 49 48
SfoI GGC'GCC
Cuts at: 0 5 2028
Size: 5 2023
SmlI C'TyrA_G
Cuts at: 0 1138 2028
Size: 1138 890
StuI AGG'CCT
Cuts at: 0 868 2028
Size: 868 1160
StyI C'CwwG_G
Cuts at: 0 335 1181 1256 1644 1715 1724 1961 2028
Size: 335 846 75 388 71 9 237 67
Fragments arranged by size:
846 388 335 237 75 71 67 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 898 1246 1893 2028
Size: 247 651 348 647 135
Fragments arranged by size:
651 647 348 247 135
TatI w'GTAC_w
Cuts at: 0 1118 1571 2028
Size: 1118 453 457
Fragments arranged by size:
1118 457 453
Tth111I GACn'n_nGTC
Cuts at: 0 532 2028
Size: 532 1496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1731 2028
Size: 1731 297
XmnI GAAnn'nnTTC
Cuts at: 0 1495 2028
Size: 1495 533
Enzymes that do cut:
AccI AcuI AfeI AhdI AlfI AloI AlwNI ApaLI
ApoI AscI BanI BanII BbeI BbsI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NarI NcoI
NsiI NspI PflMI PfoI PpiI PpuMI PstI SacII
SalI SbfI ScaI SfcI SfoI SmlI StuI StyI
TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
ApaI AseI AsiSI AvaI AvrII BaeI BamHI BcgI
BclI BmrI BmtI BsaI BsaAI BsaBI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssSI BstBI
BstZ17I ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspAI HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SanDI SapI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XhoI XmaI ZraI