Restriction map for
pFN21AB6228
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 980 GACGTC GACGT^C
AccI 373 GTCTAC GT^[AC][GT]AC
Asp700I 192 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 341 CTCGGG C^[CT]CG[GA]G
BbeI 1076 GGCGCC GGCGC^C
BbsI 1312 GAAGAC GAAGAC(2/6)
BciVI 1728 GGATAC GTATCC(6/5)
BglI 2139 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 58 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 58 GCTCAGC GC^T[ACGT]AGC
BpuAI 1312 GAAGAC GAAGAC(2/6)
BsaI 1821 GGTCTC GGTCTC(1/5)
BsaWI 1091 ACCGGA [AT]^CCGG[AT]
BsiEI 747 CGGCCG CG[GA][CT]^CG
BsoBI 341 CTCGGG C^[CT]CG[GA]G
BsrBI 814 GAGCGG CCGCTC(-3/-3)
BssSI 2049 CACGAG CACGAG(-5/-1)
BstAPI 164 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 199 TTCGAA TT^CGAA
BstEII 2079 GGTGACC G^GT[ACGT]ACC
BstPI 2079 GGTGACC G^GT[ACGT]ACC
BstXI 2048 CCACGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 58 GCTCAGC GC^T[ACGT]AGC
EagI 747 CGGCCG C^GGCCG
EclXI 747 CGGCCG C^GGCCG
Eco52I 747 CGGCCG C^GGCCG
EcoO65I 2079 GGTGACC G^GT[ACGT]ACC
FauI 785 CCCGC CCCGC(4/6)
HindIII 1715 AAGCTT A^AGCTT
HpaI 799 GTTAAC GTT^AAC
KasI 1076 GGCGCC G^GCGCC
MseI 800 TTAA T^TAA
NaeI 353 GCCGGC GCC^GGC
NarI 1076 GGCGCC GG^CGCC
NcoI 2070 CCATGG C^CATGG
NgoMIV 353 GCCGGC G^CCGGC
NspV 199 TTCGAA TT^CGAA
SbfI 666 CCTGCAGG CCTGCA^GG
SfoI 1076 GGCGCC GGC^GCC
SfuI 199 TTCGAA TT^CGAA
SmlI 400 CTCAAG C^T[CT][GA]AG
Sse8387I 666 CCTGCAGG CCTGCA^GG
Tru9I 800 TTAA T^TAA
Tsp509I 641 AATT ^AATT
XmnI 192 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 1991 GTAC GT^AC
2153 GTAC
AocI 244 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
BglII 1502 AGATCT A^GATCT
1546 AGATCT
BsgI 390 CTGCAC GTGCAG(16/14)
1049 CTGCAC
BsmBI 1848 CGTCTC CGTCTC(1/5)
1747 GAGACG
BspMI 665 ACCTGC ACCTGC(4/8)
1462 GCAGGT
BsrDI 1138 GCAATG GCAATG(2/0)
1640 GCAATG
BsrI 1862 ACTGG ACTGG(1/-1)
2032 CCAGT
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
HaeII 585 GGCGCT [GA]GCGC^[CT]
1076 GGCGCC
HgaI 1574 GACGC GACGC(5/10)
1847 GCGTC
HincII 799 GTTAAC GT[CT]^[GA]AC
1042 GTTGAC
HindII 799 GTTAAC GT[CT]^[GA]AC
1042 GTTGAC
MaeII 279 ACGT A^CGT
981 ACGT
MslI 18 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2049 CACGAGGGTG
PpuMI 332 GGGTCCT [GA]G^G[AT]CC[CT]
538 AGGACCC
RsaI 1991 GTAC GT^AC
2153 GTAC
SacI 385 GAGCTC GAGCT^C
1184 GAGCTC
SapI 1181 GAAGAGC GCTCTTC(1/4)
734 GCTCTTC
TaqI 200 TCGA T^CGA
989 TCGA
TthHB8I 200 TCGA T^CGA
989 TCGA
XcmI 1103 CCATGAGTCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2066 CCAGCCATGGCCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
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AciI AcyI AluI AlwI AlwNI
ApaI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
TspRI Van91I XhoII