(Circular) (Six-base) MAPSORT of: pFN21AB6228.seq Check: 3879 from: 1 to: 6925 pFN21AB6228 6925 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2989 4735 278 Size: 53 83 186 936 1453 1746 2468 Fragments arranged by size: 2468 1746 1453 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2379 4268 1937 Size: 442 1889 4594 Fragments arranged by size: 4594 1889 442 Acc65I G'GTAC_C Cuts at: 4246 4246 Size: 6925 AclI AA'CG_TT Cuts at: 1563 5052 5425 1563 Size: 3489 373 3063 Fragments arranged by size: 3489 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3408 3540 4988 6372 6640 1632 Size: 1776 132 1448 1384 268 1917 Fragments arranged by size: 1917 1776 1448 1384 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 5824 6801 1203 Size: 4621 977 1327 Fragments arranged by size: 4621 1327 977 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 5654 5654 Size: 6925 AleI CACnn'nnGTG Cuts at: 1166 4058 1166 Size: 2892 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 2301 1184 Size: 861 256 5808 Fragments arranged by size: 5808 861 256 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2536 2568 2536 Size: 32 6893 AlwNI CAG_nnn'CTG Cuts at: 2587 2843 3068 3386 4043 4220 4668 6240 2587 Size: 256 225 318 657 177 448 1572 3272 Fragments arranged by size: 3272 1572 657 448 318 256 225 177 ApaI G_GGCC'C Cuts at: 2076 2353 3260 2076 Size: 277 907 5741 Fragments arranged by size: 5741 907 277 ApaLI G'TGCA_C Cuts at: 4982 6138 4982 Size: 1156 5769 ApoI r'AATT_y Cuts at: 1493 4234 4460 4612 6721 1493 Size: 2741 226 152 2109 1697 Fragments arranged by size: 2741 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5479 160 Size: 872 4447 1606 Fragments arranged by size: 4447 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2346 4250 1870 Size: 73 403 1904 4545 Fragments arranged by size: 4545 1904 403 73 BamHI G'GATC_C Cuts at: 4255 4622 4255 Size: 367 6558 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2786 3081 3203 3282 4246 Size: 325 217 627 999 295 122 79 964 Cuts at: 4246 5701 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 999 964 627 325 295 217 122 79 BanII G_rGCy'C Cuts at: 1391 2076 2353 2394 2469 3193 3260 3491 3792 Size: 685 277 41 75 724 67 231 301 Cuts at: 3792 4244 1391 Size: 452 4072 Fragments arranged by size: 4072 724 685 452 301 277 231 75 67 41 BbeI G_GCGC'C Cuts at: 3085 3085 Size: 6925 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3324 928 Size: 971 1425 4529 Fragments arranged by size: 4529 1425 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5116 5150 1903 Size: 34 3179 34 3678 Fragments arranged by size: 3678 3179 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3727 4817 6027 3727 Size: 1090 1210 4625 Fragments arranged by size: 4625 1210 1090 BclI T'GATC_A Cuts at: 1548 1548 Size: 6925 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 5536 136 Size: 107 122 71 631 605 2478 1386 1525 Fragments arranged by size: 2478 1525 1386 631 605 122 107 71 BglII A'GATC_T Cuts at: 3507 3551 6920 3507 Size: 44 3369 3512 Fragments arranged by size: 3512 3369 44 BlpI GC'TnA_GC Cuts at: 2064 4342 2064 Size: 2278 4647 Bme1580I G_kGCm'C Cuts at: 1790 2076 2290 2353 3260 3287 4986 6142 1790 Size: 286 214 63 907 27 1699 1156 2573 Fragments arranged by size: 2573 1699 1156 907 286 214 63 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3877 4031 4656 Size: 416 53 83 399 24 2460 154 625 Cuts at: 4656 5614 442 Size: 958 1753 Fragments arranged by size: 2460 1753 958 625 416 399 154 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6925 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3207 3212 3148 Size: 59 5 6861 Fragments arranged by size: 6861 59 5 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2915 3280 3610 3695 4095 5585 1239 Size: 968 708 365 330 85 400 1490 2579 Fragments arranged by size: 2579 1490 968 708 400 365 330 85 Bpu10I CC'TnA_GC Cuts at: 2943 2943 Size: 6925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 5050 5915 6213 2390 Size: 2660 865 298 3102 Fragments arranged by size: 3102 2660 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3832 5588 882 Size: 617 2333 1756 2219 Fragments arranged by size: 2333 2219 1756 617 BsaAI yAC'GTr Cuts at: 493 1204 4716 493 Size: 711 3512 2702 Fragments arranged by size: 3512 2702 711 BsaBI GATnn'nnATC Cuts at: 6919 6919 Size: 6925 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2986 3082 3852 4732 Size: 53 83 186 936 1453 96 770 880 Cuts at: 4732 5114 5749 275 Size: 382 635 1451 Fragments arranged by size: 1453 1451 936 880 770 635 382 186 96 83 53 BsaWI w'CCGG_w Cuts at: 3096 5358 5756 6030 6177 6588 3096 Size: 2262 398 274 147 411 3433 Fragments arranged by size: 3433 2262 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2645 2669 2675 2699 2913 Size: 30 165 30 1242 24 6 24 214 Cuts at: 2913 2943 1178 Size: 30 5160 Fragments arranged by size: 5160 1242 214 165 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 3179 3341 3359 3362 3365 3368 3383 Size: 930 1063 162 18 3 3 3 15 Cuts at: 3383 3386 3407 3410 3455 3677 3800 3849 4137 Size: 3 21 3 45 222 123 49 288 Cuts at: 4137 1186 Size: 3974 Fragments arranged by size: 3974 1063 930 288 222 162 123 49 45 21 18 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 4004 4070 6128 6624 4004 Size: 66 2058 496 4305 Fragments arranged by size: 4305 2058 496 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2380 3039 1163 Size: 573 644 659 5049 Fragments arranged by size: 5049 659 644 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2755 4375 5136 6164 1540 Size: 275 186 754 1620 761 1028 2301 Fragments arranged by size: 2301 1620 1028 761 754 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 3193 4212 4986 5071 6142 2394 Size: 799 1019 774 85 1071 3177 Fragments arranged by size: 3177 1071 1019 799 774 85 BsmI GAATG_Cn' Cuts at: 4436 4529 4436 Size: 93 6832 BsmBI CGTCTCn'nnnn_ Cuts at: 3746 3859 3746 Size: 113 6812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2290 2353 2394 2469 3193 3260 Size: 399 286 214 63 41 75 724 67 Cuts at: 3260 3287 3491 3792 4212 4244 4986 5071 6142 Size: 27 204 301 420 32 742 85 1071 Cuts at: 6142 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 724 420 399 301 286 214 204 85 75 67 63 41 32 27 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 4814 6544 4814 Size: 1730 5195 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2679 3458 4280 844 Size: 1835 779 822 3489 Fragments arranged by size: 3489 1835 822 779 BsrBI CCG'CTC Cuts at: 1394 1823 2821 4812 1394 Size: 429 998 1991 3507 Fragments arranged by size: 3507 1991 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3150 3652 5420 5594 65 Size: 1183 1902 502 1768 174 1396 Fragments arranged by size: 1902 1768 1396 1183 502 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2274 2358 2750 5569 5756 1700 Size: 110 464 84 392 2819 187 2869 Fragments arranged by size: 2869 2819 464 392 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 4054 4985 5997 4054 Size: 931 1012 4982 Fragments arranged by size: 4982 1012 931 BstAPI GCAn_nnn'nTGC Cuts at: 794 2175 794 Size: 1381 5544 BstBI TT'CG_AA Cuts at: 2205 5729 2205 Size: 3524 3401 BstEII G'GTnAC_C Cuts at: 1184 4084 1184 Size: 2900 4025 BstXI CCAn_nnnn'nTGG Cuts at: 4060 4060 Size: 6925 BstYI r'GATC_y Cuts at: 1972 2565 2607 2635 3064 3400 3507 3551 4255 Size: 593 42 28 429 336 107 44 704 Cuts at: 4255 4622 5007 5024 6476 6562 6574 6920 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 704 593 429 385 367 346 336 107 86 44 42 28 17 12 Bsu36I CC'TnA_GG Cuts at: 2250 3660 2250 Size: 1410 5515 BtgI C'CryG_G Cuts at: 513 1065 2004 2548 2782 4075 513 Size: 552 939 544 234 1293 3363 Fragments arranged by size: 3363 1293 939 552 544 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6186 Fragments arranged by size: 6186 683 56 BtsI GCAGTG_nn' Cuts at: 2523 4441 5235 5255 2523 Size: 1918 794 20 4193 Fragments arranged by size: 4193 1918 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6925 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6890 DraI TTT'AAA Cuts at: 4229 4229 Size: 6925 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2360 2752 Size: 54 1425 50 275 18 177 353 392 Cuts at: 2752 3105 4176 4372 5261 6890 8 Size: 353 1071 196 889 1629 43 Fragments arranged by size: 1629 1425 1071 889 392 353 353 275 196 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2752 4372 1537 Size: 275 940 1620 4090 Fragments arranged by size: 4090 1620 940 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2202 2746 3181 3214 3336 3360 3384 3432 Size: 849 544 435 33 122 24 24 48 Cuts at: 3432 3444 3772 3835 4191 4855 1353 Size: 12 328 63 356 664 3423 Fragments arranged by size: 3423 849 664 544 435 356 328 122 63 48 33 24 24 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2327 2693 5512 5886 6032 387 Size: 1064 138 738 366 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 738 374 366 146 138 EcoICRI GAG'CTC Cuts at: 2392 3191 2392 Size: 799 6126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2915 3280 3408 3540 3610 3695 Size: 393 575 708 365 128 132 70 85 Cuts at: 3695 4095 4988 5585 6372 6640 1239 Size: 400 893 597 787 268 1524 Fragments arranged by size: 1524 893 787 708 597 575 400 393 365 268 132 128 85 70 EcoNI CCTnn'n_nnAGG Cuts at: 2675 3769 4054 2675 Size: 1094 285 5546 Fragments arranged by size: 5546 1094 285 EcoO109I rG'GnC_Cy Cuts at: 2072 2073 2338 2544 2840 3256 3257 2072 Size: 1 265 206 296 416 1 5740 Fragments arranged by size: 5740 416 296 265 206 1 1 EcoRI G'AATT_C Cuts at: 4234 4612 4234 Size: 378 6547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2231 2452 2231 Size: 221 6704 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 2594 3085 6072 1678 Size: 916 491 2987 2531 Fragments arranged by size: 2987 2531 916 491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2645 2677 2913 2945 2976 Size: 32 135 32 1073 32 236 32 31 Cuts at: 2976 3008 3749 3781 5566 5598 5640 5672 1373 Size: 32 741 32 1785 32 42 32 2626 Fragments arranged by size: 2626 1785 1073 741 236 135 42 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2806 3049 4269 4515 669 Size: 988 281 868 243 1220 246 3079 Fragments arranged by size: 3079 1220 988 868 281 246 243 HindIII A'AGCT_T Cuts at: 748 3720 748 Size: 2972 3953 HpaI GTT'AAC Cuts at: 2806 4515 2806 Size: 1709 5216 KasI G'GCGC_C Cuts at: 3081 3081 Size: 6925 KpnI G_GTAC'C Cuts at: 4250 4250 Size: 6925 MfeI C'AATT_G Cuts at: 4524 4524 Size: 6925 MluI A'CGCG_T Cuts at: 6801 6801 Size: 6925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2613 6039 6223 1305 Size: 1308 3426 184 2007 Fragments arranged by size: 3426 2007 1308 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3107 4178 10 Size: 54 1425 343 177 1098 1071 2757 Fragments arranged by size: 2757 1425 1098 1071 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 4058 4883 5242 5401 518 Size: 648 861 2031 825 359 159 2042 Fragments arranged by size: 2042 2031 861 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 2584 2894 4341 5018 6166 6411 1606 Size: 454 524 310 1447 677 1148 245 2120 Fragments arranged by size: 2120 1447 1148 677 524 454 310 245 NaeI GCC'GGC Cuts at: 2360 2360 Size: 6925 NarI GG'CG_CC Cuts at: 3082 3082 Size: 6925 NcoI C'CATG_G Cuts at: 513 1065 2004 4075 513 Size: 552 939 2071 3363 Fragments arranged by size: 3363 2071 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6925 NgoMIV G'CCGG_C Cuts at: 2358 2358 Size: 6925 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6925 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NspI r_CATG'y Cuts at: 1132 1229 4283 5828 1132 Size: 97 3054 1545 2229 Fragments arranged by size: 3054 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3277 1348 Size: 1929 4996 PciI A'CATG_T Cuts at: 5824 5824 Size: 6925 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 2938 4081 1108 Size: 1467 363 1143 3952 Fragments arranged by size: 3952 1467 1143 363 PfoI T'CCnGG_A Cuts at: 3770 3770 Size: 6925 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2186 2218 4994 5026 6507 6539 2186 Size: 32 2776 32 1481 32 2572 Fragments arranged by size: 2776 2572 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2338 2544 2338 Size: 206 6719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6925 PsiI TTA'TAA Cuts at: 4495 4495 Size: 6925 PspOMI G'GGCC_C Cuts at: 2072 2349 3256 2072 Size: 277 907 5741 Fragments arranged by size: 5741 907 277 PstI C_TGCA'G Cuts at: 830 2622 2676 3223 4277 830 Size: 1792 54 547 1054 3478 Fragments arranged by size: 3478 1792 1054 547 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 PvuII CAG'CTG Cuts at: 2060 2584 2894 2060 Size: 524 310 6091 Fragments arranged by size: 6091 524 310 SacI G_AGCT'C Cuts at: 2394 3193 2394 Size: 799 6126 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SapI GCTCTTCn'nnn_ Cuts at: 2746 3181 2746 Size: 435 6490 SbfI CC_TGCA'GG Cuts at: 2676 4277 2676 Size: 1601 5324 ScaI AGT'ACT Cuts at: 5173 6885 5173 Size: 1712 5213 SfcI C'TryA_G Cuts at: 826 1045 2618 2672 2880 3034 3219 3503 4273 Size: 219 1573 54 208 154 185 284 770 Cuts at: 4273 5408 6089 6280 826 Size: 1135 681 191 1471 Fragments arranged by size: 1573 1471 1135 770 681 284 219 208 191 185 154 54 SfoI GGC'GCC Cuts at: 3083 3083 Size: 6925 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 4252 1872 Size: 2380 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 5029 5930 6192 820 Size: 197 926 462 2624 901 262 1553 Fragments arranged by size: 2624 1553 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 4716 493 Size: 4223 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 4283 1132 Size: 3151 3774 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6925 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 2540 2908 3199 3240 4020 Size: 552 939 495 41 368 291 41 780 Cuts at: 4020 4029 4075 4364 513 Size: 9 46 289 3074 Fragments arranged by size: 3074 939 780 552 495 368 291 289 46 41 41 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 4952 4969 5122 5307 5752 325 Size: 1255 517 2855 17 153 185 445 1498 Fragments arranged by size: 2855 1498 1255 517 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5171 6883 96 Size: 274 80 33 51 1443 3194 1712 138 Fragments arranged by size: 3194 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3570 3602 6885 6917 3570 Size: 32 3283 32 3578 Fragments arranged by size: 3578 3283 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 4261 4261 Size: 6925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3115 4078 3115 Size: 963 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6925 XmaI C'CCGG_G Cuts at: 1870 4250 1870 Size: 2380 4545 XmnI GAAnn'nnTTC Cuts at: 2201 5054 2201 Size: 2853 4072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2987 4733 276 Size: 53 83 186 936 1453 1746 2468 Fragments arranged by size: 2468 1746 1453 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI