Restriction map for
pFN21AB6228
(Linear) (Six-base) MAPSORT of: KIBB6228.seq Check: 7148 from: 1 to: 2220
KIBB6228.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 984 2220
Size: 984 1236
AccI GT'mk_AC
Cuts at: 0 374 2220
Size: 374 1846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1535 2220
Size: 1403 132 685
Fragments arranged by size:
1403 685 132
AleI CACnn'nnGTG
Cuts at: 0 2053 2220
Size: 2053 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 296 2220
Size: 40 256 1924
Fragments arranged by size:
1924 256 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 2220
Size: 531 32 1657
Fragments arranged by size:
1657 531 32
AlwNI CAG_nnn'CTG
Cuts at: 0 582 838 1063 1381 2038 2215 2220
Size: 582 256 225 318 657 177 5
Fragments arranged by size:
657 582 318 256 225 177 5
ApaI G_GGCC'C
Cuts at: 0 71 348 1255 2220
Size: 71 277 907 965
Fragments arranged by size:
965 907 277 71
AvaI C'yCGr_G
Cuts at: 0 341 2220
Size: 341 1879
BanI G'GyrC_C
Cuts at: 0 781 1076 1198 1277 2220
Size: 781 295 122 79 943
Fragments arranged by size:
943 781 295 122 79
BanII G_rGCy'C
Cuts at: 0 71 348 389 464 1188 1255 1486 1787
Size: 71 277 41 75 724 67 231 301
Cuts at: 1787 2220
Size: 433
Fragments arranged by size:
724 433 301 277 231 75 71 67
41
BbeI G_GCGC'C
Cuts at: 0 1080 2220
Size: 1080 1140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1319 2220
Size: 1319 901
BciVI GTATCCnnnnn_n'
Cuts at: 0 1722 2220
Size: 1722 498
BglI GCCn_nnn'nGGC
Cuts at: 0 2145 2220
Size: 2145 75
BglII A'GATC_T
Cuts at: 0 1502 1546 2220
Size: 1502 44 674
Fragments arranged by size:
1502 674 44
BlpI GC'TnA_GC
Cuts at: 0 59 2220
Size: 59 2161
Bme1580I G_kGCm'C
Cuts at: 0 71 285 348 1255 1282 2220
Size: 71 214 63 907 27 938
Fragments arranged by size:
938 907 214 71 63 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1872 2026 2220
Size: 1872 154 194
Fragments arranged by size:
1872 194 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 1202 1207 2220
Size: 1143 59 5 1013
Fragments arranged by size:
1143 1013 59 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 910 1275 1605 1690 2090 2220
Size: 202 708 365 330 85 400 130
Fragments arranged by size:
708 400 365 330 202 130 85
Bpu10I CC'TnA_GC
Cuts at: 0 938 2220
Size: 938 1282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 2220
Size: 385 1835
BsaI GGTCTCn'nnnn_
Cuts at: 0 1827 2220
Size: 1827 393
BsaHI Gr'CG_yC
Cuts at: 0 981 1077 1847 2220
Size: 981 96 770 373
Fragments arranged by size:
981 770 373 96
BsaWI w'CCGG_w
Cuts at: 0 1091 2220
Size: 1091 1129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 640 664 670 694 908 938 2220
Size: 640 24 6 24 214 30 1282
Fragments arranged by size:
1282 640 214 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 1174 1336 1354 1357 1360 1363 1378
Size: 111 1063 162 18 3 3 3 15
Cuts at: 1378 1381 1402 1405 1450 1672 1795 1844 2132
Size: 3 21 3 45 222 123 49 288
Cuts at: 2132 2220
Size: 88
Fragments arranged by size:
1063 288 222 162 123 111 88 49
45 21 18 15 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 1999 2065 2220
Size: 1999 66 155
Fragments arranged by size:
1999 155 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 1034 2220
Size: 375 659 1186
Fragments arranged by size:
1186 659 375
BsiEI CG_ry'CG
Cuts at: 0 750 2220
Size: 750 1470
BsiHKAI G_wGCw'C
Cuts at: 0 389 1188 2207 2220
Size: 389 799 1019 13
Fragments arranged by size:
1019 799 389 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1741 1854 2220
Size: 1741 113 366
Fragments arranged by size:
1741 366 113
Bsp1286I G_dGCh'C
Cuts at: 0 71 285 348 389 464 1188 1255 1282
Size: 71 214 63 41 75 724 67 27
Cuts at: 1282 1486 1787 2207 2220
Size: 204 301 420 13
Fragments arranged by size:
724 420 301 214 204 75 71 67
63 41 27 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 674 1453 2220
Size: 674 779 767
Fragments arranged by size:
779 767 674
BsrBI CCG'CTC
Cuts at: 0 816 2220
Size: 816 1404
BsrDI GCAATG_nn'
Cuts at: 0 1145 1647 2220
Size: 1145 502 573
Fragments arranged by size:
1145 573 502
BsrFI r'CCGG_y
Cuts at: 0 269 353 745 2220
Size: 269 84 392 1475
Fragments arranged by size:
1475 392 269 84
BssSI C'ACGA_G
Cuts at: 0 2049 2220
Size: 2049 171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 170 2220
Size: 170 2050
BstBI TT'CG_AA
Cuts at: 0 200 2220
Size: 200 2020
BstEII G'GTnAC_C
Cuts at: 0 2079 2220
Size: 2079 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2055 2220
Size: 2055 165
BstYI r'GATC_y
Cuts at: 0 560 602 630 1059 1395 1502 1546 2220
Size: 560 42 28 429 336 107 44 674
Fragments arranged by size:
674 560 429 336 107 44 42 28
Bsu36I CC'TnA_GG
Cuts at: 0 245 1655 2220
Size: 245 1410 565
Fragments arranged by size:
1410 565 245
BtgI C'CryG_G
Cuts at: 0 543 777 2070 2220
Size: 543 234 1293 150
Fragments arranged by size:
1293 543 234 150
BtsI GCAGTG_nn'
Cuts at: 0 518 2220
Size: 518 1702
EaeI y'GGCC_r
Cuts at: 0 2 355 747 1100 2171 2220
Size: 2 353 392 353 1071 49
Fragments arranged by size:
1071 392 353 353 49 2
EagI C'GGCC_G
Cuts at: 0 747 2220
Size: 747 1473
EarI CTCTTCn'nnn_
Cuts at: 0 197 741 1176 1209 1331 1355 1379 1427
Size: 197 544 435 33 122 24 24 48
Cuts at: 1427 1439 1767 1830 2186 2220
Size: 12 328 63 356 34
Fragments arranged by size:
544 435 356 328 197 122 63 48
34 33 24 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 322 688 2220
Size: 322 366 1532
Fragments arranged by size:
1532 366 322
EcoICRI GAG'CTC
Cuts at: 0 387 1186 2220
Size: 387 799 1034
Fragments arranged by size:
1034 799 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 910 1275 1403 1535 1605 1690 2090
Size: 202 708 365 128 132 70 85 400
Cuts at: 2090 2220
Size: 130
Fragments arranged by size:
708 400 365 202 132 130 128 85
70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 1764 2049 2220
Size: 670 1094 285 171
Fragments arranged by size:
1094 670 285 171
EcoO109I rG'GnC_Cy
Cuts at: 0 67 68 333 539 835 1251 1252 2220
Size: 67 1 265 206 296 416 1 968
Fragments arranged by size:
968 416 296 265 206 67 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 226 447 2220
Size: 226 221 1773
Fragments arranged by size:
1773 226 221
HaeII r_GCGC'y
Cuts at: 0 589 1080 2220
Size: 589 491 1140
Fragments arranged by size:
1140 589 491
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 640 672 908 940 971 1003 1744 1776
Size: 640 32 236 32 31 32 741 32
Cuts at: 1776 2220
Size: 444
Fragments arranged by size:
741 640 444 236 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 801 1044 2220
Size: 801 243 1176
Fragments arranged by size:
1176 801 243
HindIII A'AGCT_T
Cuts at: 0 1715 2220
Size: 1715 505
HpaI GTT'AAC
Cuts at: 0 801 2220
Size: 801 1419
KasI G'GCGC_C
Cuts at: 0 1076 2220
Size: 1076 1144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 2220
Size: 608 1612
MscI TGG'CCA
Cuts at: 0 4 1102 2173 2220
Size: 4 1098 1071 47
Fragments arranged by size:
1098 1071 47 4
MslI CAynn'nnrTG
Cuts at: 0 22 2053 2220
Size: 22 2031 167
Fragments arranged by size:
2031 167 22
MspA1I CmG'CkG
Cuts at: 0 55 579 889 2220
Size: 55 524 310 1331
Fragments arranged by size:
1331 524 310 55
NaeI GCC'GGC
Cuts at: 0 355 2220
Size: 355 1865
NarI GG'CG_CC
Cuts at: 0 1077 2220
Size: 1077 1143
NcoI C'CATG_G
Cuts at: 0 2070 2220
Size: 2070 150
NgoMIV G'CCGG_C
Cuts at: 0 353 2220
Size: 353 1867
PasI CC'CwG_GG
Cuts at: 0 1272 2220
Size: 1272 948
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 933 2076 2220
Size: 570 363 1143 144
Fragments arranged by size:
1143 570 363 144
PfoI T'CCnGG_A
Cuts at: 0 1765 2220
Size: 1765 455
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 2220
Size: 181 32 2007
Fragments arranged by size:
2007 181 32
PpuMI rG'GwC_Cy
Cuts at: 0 333 539 2220
Size: 333 206 1681
Fragments arranged by size:
1681 333 206
PspOMI G'GGCC_C
Cuts at: 0 67 344 1251 2220
Size: 67 277 907 969
Fragments arranged by size:
969 907 277 67
PstI C_TGCA'G
Cuts at: 0 617 671 1218 2220
Size: 617 54 547 1002
Fragments arranged by size:
1002 617 547 54
PvuII CAG'CTG
Cuts at: 0 55 579 889 2220
Size: 55 524 310 1331
Fragments arranged by size:
1331 524 310 55
SacI G_AGCT'C
Cuts at: 0 389 1188 2220
Size: 389 799 1032
Fragments arranged by size:
1032 799 389
SapI GCTCTTCn'nnn_
Cuts at: 0 741 1176 2220
Size: 741 435 1044
Fragments arranged by size:
1044 741 435
SbfI CC_TGCA'GG
Cuts at: 0 671 2220
Size: 671 1549
SfcI C'TryA_G
Cuts at: 0 613 667 875 1029 1214 1498 2220
Size: 613 54 208 154 185 284 722
Fragments arranged by size:
722 613 284 208 185 154 54
SfoI GGC'GCC
Cuts at: 0 1078 2220
Size: 1078 1142
SmlI C'TyrA_G
Cuts at: 0 400 2220
Size: 400 1820
StyI C'CwwG_G
Cuts at: 0 494 535 903 1194 1235 2015 2024 2070
Size: 494 41 368 291 41 780 9 46
Cuts at: 2070 2220
Size: 150
Fragments arranged by size:
780 494 368 291 150 46 41 41
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 2220
Size: 92 2128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1565 1597 2220
Size: 1565 32 623
Fragments arranged by size:
1565 623 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 2073 2220
Size: 1110 963 147
Fragments arranged by size:
1110 963 147
XmnI GAAnn'nnTTC
Cuts at: 0 196 2220
Size: 196 2024
ZraI GAC'GTC
Cuts at: 0 982 2220
Size: 982 1238
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AloI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BciVI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SbfI SfcI SfoI SmlI StyI TaqII TstI XcmI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BclI BfrBI BmgBI BmtI BsaAI BsaBI
BsiWI BsmI BspEI BspHI BsrGI BssHII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI
FspI FspAI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI