Restriction map for
pFN21AB6215
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
BciVI BclI BfrI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eam1105I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KspI MamI MfeI MluI MroI
MseI MunI NcoI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 1606 AAATTC [GA]^AATT[CT]
AflIII 1965 ACGTGT A^C[GA][CT]GT
ApaI 1050 GGGCCC GGGCC^C
ApoI 1606 AAATTC [GA]^AATT[CT]
AviII 491 TGCGCA TGC^GCA
AvrII 684 CCTAGG C^CTAGG
BalI 1130 TGGCCA TGG^CCA
BbeI 831 GGCGCC GGCGC^C
BbvCI 190 CCTCAGC CCTCAGC(-5/-2)
BcgI 44 GCAGCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 685 CTAG C^TAG
BglII 230 AGATCT A^GATCT
BlnI 684 CCTAGG C^CTAGG
BsrDI 1559 GCAATG GCAATG(2/0)
BssHII 17 GCGCGC G^CGCGC
BstEII 1303 GGTGACC G^GT[ACGT]ACC
BstPI 1303 GGTGACC G^GT[ACGT]ACC
BstXI 521 CCACGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1478 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 142 GACGCGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1303 GGTGACC G^GT[ACGT]ACC
FseI 973 GGCCGGCC GGCCGG^CC
FspI 491 TGCGCA TGC^GCA
HinfI 1181 GAGTC G^A[ACGT]TC
KasI 831 GGCGCC G^GCGCC
MaeI 685 CTAG C^TAG
MluNI 1130 TGGCCA TGG^CCA
MscI 1130 TGGCCA TGG^CCA
NaeI 974 GCCGGC GCC^GGC
NarI 831 GGCGCC GG^CGCC
NdeI 205 CATATG CA^TATG
NgoMIV 974 GCCGGC G^CCGGC
NotI 75 GCGGCCGC GC^GGCCGC
PleI 1181 GAGTC GAGTC(4/5)
PstI 1993 CTGCAG CTGCA^G
SfcI 1993 CTGCAG C^T[GA][CT]AG
SfoI 831 GGCGCC GGC^GCC
SmaI 1753 CCCGGG CCC^GGG
Tsp509I 1607 AATT ^AATT
XmaCI 1753 CCCGGG C^CCGGG
XmaI 1753 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AgeI 283 ACCGGT A^CCGGT
643 ACCGGT
AvaII 150 GGTCC G^G[AT]CC
722 GGACC
BamHI 272 GGATCC G^GATCC
1526 GGATCC
BbrPI 1684 CACGTG CAC^GTG
1964 CACGTG
BbsI 139 GAAGAC GAAGAC(2/6)
532 GTCTTC
BpuAI 139 GAAGAC GAAGAC(2/6)
532 GTCTTC
BsaAI 1684 CACGTG [CT]AC^GT[GA]
1964 CACGTG
BsaI 301 GAGACC GGTCTC(1/5)
1487 GAGACC
BsaWI 283 ACCGGT [AT]^CCGG[AT]
643 ACCGGT
BsrBI 1033 GAGCGG CCGCTC(-3/-3)
1078 GAGCGG
DdeI 191 CTCAG C^T[ACGT]AG
1555 CTGAG
EarI 757 CTCTTC CTCTTC(1/4)
298 GAAGAG
HincII 856 GTTGAC GT[CT]^[GA]AC
1813 GTCAAC
HindII 856 GTTGAC GT[CT]^[GA]AC
1813 GTCAAC
Ksp632I 757 CTCTTC CTCTTC(1/4)
298 GAAGAG
MslI 282 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1738 CACGGGGGTG
PinAI 283 ACCGGT A^CCGGT
643 ACCGGT
PmaCI 1684 CACGTG CAC^GTG
1964 CACGTG
PmlI 1684 CACGTG CAC^GTG
1964 CACGTG
SfiI 968 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
989 GGCCGCCCCGGCC
SgrAI 282 CACCGGTG C[GA]^CCGG[CT]G
642 CACCGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI Aor51HI Asp718I
AspHI AvaI BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI KpnI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII