(Circular) (Six-base) MAPSORT of: pFN21AB6215.seq Check: 6323 from: 1 to: 6712 pFN21AB6215 6712 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4055 1937 Size: 2118 4594 Acc65I G'GTAC_C Cuts at: 2475 2915 3501 4033 2475 Size: 440 586 532 5154 Fragments arranged by size: 5154 586 532 440 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4775 6159 6427 1632 Size: 3143 1384 268 1917 Fragments arranged by size: 3143 1917 1384 268 AfeI AGC'GCT Cuts at: 2267 2396 3992 2267 Size: 129 1596 4987 Fragments arranged by size: 4987 1596 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 3970 5611 6588 1203 Size: 2767 1641 977 1327 Fragments arranged by size: 2767 1641 1327 977 AgeI A'CCGG_T Cuts at: 2288 2648 5543 2288 Size: 360 2895 3457 Fragments arranged by size: 3457 2895 360 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 2291 3747 1166 Size: 1125 1456 4131 Fragments arranged by size: 4131 1456 1125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2762 1184 Size: 1578 5134 AlwNI CAG_nnn'CTG Cuts at: 3364 3462 3522 4455 6027 3364 Size: 98 60 933 1572 4049 Fragments arranged by size: 4049 1572 933 98 60 ApaI G_GGCC'C Cuts at: 3059 3059 Size: 6712 ApaLI G'TGCA_C Cuts at: 4769 5925 4769 Size: 1156 5556 ApoI r'AATT_y Cuts at: 1493 3611 4021 4247 4399 6508 1493 Size: 2118 410 226 152 2109 1697 Fragments arranged by size: 2118 2109 1697 410 226 152 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2879 3072 3758 4037 1870 Size: 73 74 862 193 686 279 4545 Fragments arranged by size: 4545 862 686 279 193 74 73 AvrII C'CTAG_G Cuts at: 2689 2689 Size: 6712 BamHI G'GATC_C Cuts at: 2277 3531 4042 4409 2277 Size: 1254 511 367 4580 Fragments arranged by size: 4580 1254 511 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2341 2475 2548 2645 2836 Size: 325 217 627 554 134 73 97 191 Cuts at: 2836 2915 3501 3596 3895 4033 5488 618 Size: 79 586 95 299 138 1455 1842 Fragments arranged by size: 1842 1455 627 586 554 325 299 217 191 138 134 97 95 79 73 BanII G_rGCy'C Cuts at: 1391 2724 3059 3348 3426 3642 3759 4031 1391 Size: 1333 335 289 78 216 117 272 4072 Fragments arranged by size: 4072 1333 335 289 272 216 117 78 BbeI G_GCGC'C Cuts at: 2840 2840 Size: 6712 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 2530 928 Size: 971 252 379 5110 Fragments arranged by size: 5110 971 379 252 BbvCI CC'TCA_GC Cuts at: 2196 2196 Size: 6712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2038 2072 4903 4937 1903 Size: 34 101 34 2831 34 3678 Fragments arranged by size: 3678 2831 101 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 1548 Size: 6712 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 2058 2834 Size: 107 122 71 631 605 360 26 776 Cuts at: 2834 2971 2980 3001 3040 3229 5323 136 Size: 137 9 21 39 189 2094 1525 Fragments arranged by size: 2094 1525 776 631 605 360 189 137 122 107 71 39 26 21 9 BglII A'GATC_T Cuts at: 2235 6707 2235 Size: 4472 2240 BlpI GC'TnA_GC Cuts at: 4129 4129 Size: 6712 Bme1580I G_kGCm'C Cuts at: 1790 2553 3059 3234 3599 4773 5929 1790 Size: 763 506 175 365 1174 1156 2573 Fragments arranged by size: 2573 1174 1156 763 506 365 175 BmgBI CAC'GTC Cuts at: 1318 3718 3784 1318 Size: 2400 66 4246 Fragments arranged by size: 4246 2400 66 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2276 3056 4443 Size: 416 53 83 399 24 859 780 1387 Cuts at: 4443 5401 442 Size: 958 1753 Fragments arranged by size: 1753 1387 958 859 780 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2719 2797 2825 3362 5372 1239 Size: 1480 78 28 537 2010 2579 Fragments arranged by size: 2579 2010 1480 537 78 28 Bpu10I CC'TnA_GC Cuts at: 2196 3560 2196 Size: 1364 5348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4837 5702 6000 4837 Size: 865 298 5549 Fragments arranged by size: 5549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 3486 5375 882 Size: 617 801 1186 1889 2219 Fragments arranged by size: 2219 1889 1186 801 617 BsaAI yAC'GTr Cuts at: 493 1204 3691 3971 4503 493 Size: 711 2487 280 532 2702 Fragments arranged by size: 2702 2487 711 532 280 BsaBI GATnn'nnATC Cuts at: 6706 6706 Size: 6712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2386 2837 2963 4519 Size: 53 83 186 936 853 451 126 1556 Cuts at: 4519 4901 5536 275 Size: 382 635 1451 Fragments arranged by size: 1556 1451 936 853 635 451 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 2288 2648 5145 5543 5817 5964 6375 2288 Size: 360 2497 398 274 147 411 2625 Fragments arranged by size: 2625 2497 411 398 360 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6487 Fragments arranged by size: 6487 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2084 3491 3872 1186 Size: 859 39 1407 381 4026 Fragments arranged by size: 4026 1407 859 381 39 BseYI C'CCAG_C Cuts at: 2201 2785 2797 2943 3009 5915 6411 2201 Size: 584 12 146 66 2906 496 2502 Fragments arranged by size: 2906 2502 584 496 146 66 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3771 3869 3965 1163 Size: 573 2035 98 96 3910 Fragments arranged by size: 3910 2035 573 98 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 2065 2084 2142 2996 3289 Size: 275 186 35 29 19 58 854 293 Cuts at: 3289 3728 4162 4923 5951 1540 Size: 439 434 761 1028 2301 Fragments arranged by size: 2301 1028 854 761 439 434 293 275 186 58 35 29 19 BsiHKAI G_wGCw'C Cuts at: 2298 2811 3690 4773 4858 5929 2298 Size: 513 879 1083 85 1071 3081 Fragments arranged by size: 3081 1083 1071 879 513 85 BsmI GAATG_Cn' Cuts at: 4223 4316 4223 Size: 93 6619 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2553 2724 2811 3059 3234 3348 Size: 399 508 255 171 87 248 175 114 Cuts at: 3348 3426 3599 3642 3690 3759 4031 4773 4858 Size: 78 173 43 48 69 272 742 85 Cuts at: 4858 5929 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 272 255 248 175 173 171 114 87 85 78 69 48 43 BspEI T'CCGG_A Cuts at: 6375 6375 Size: 6712 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2199 2385 2754 4067 844 Size: 1355 186 369 1313 3489 Fragments arranged by size: 3489 1355 1313 369 186 BsrBI CCG'CTC Cuts at: 1394 1823 3040 3085 4599 1394 Size: 429 1217 45 1514 3507 Fragments arranged by size: 3507 1514 1217 429 45 BsrDI GCAATG_nn' Cuts at: 65 1248 3571 5207 5381 65 Size: 1183 2323 1636 174 1396 Fragments arranged by size: 2323 1636 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2288 2648 2979 2991 5356 5543 1700 Size: 110 478 360 331 12 2365 187 2869 Fragments arranged by size: 2869 2365 478 360 331 187 110 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 6615 BssSI C'ACGA_G Cuts at: 4772 5784 4772 Size: 1012 5700 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6712 BstBI TT'CG_AA Cuts at: 5516 5516 Size: 6712 BstEII G'GTnAC_C Cuts at: 1184 3308 1184 Size: 2124 4588 BstXI CCAn_nnnn'nTGG Cuts at: 2533 2533 Size: 6712 BstYI r'GATC_y Cuts at: 1972 2235 2277 3531 4042 4409 4794 4811 6263 Size: 263 42 1254 511 367 385 17 1452 Cuts at: 6263 6349 6361 6707 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1254 511 385 367 346 263 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2620 2893 2966 3742 513 Size: 552 939 616 273 73 776 3483 Fragments arranged by size: 3483 939 776 616 552 273 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5973 Fragments arranged by size: 5973 683 56 BtsI GCAGTG_nn' Cuts at: 2781 3899 4228 5022 5042 2781 Size: 1118 329 794 20 4451 Fragments arranged by size: 4451 1118 794 329 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DraIII CAC_nnn'GTG Cuts at: 3488 3488 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2153 5719 809 Size: 507 837 3566 1802 Fragments arranged by size: 3566 1802 837 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2081 2139 Size: 54 1425 50 275 18 203 48 58 Cuts at: 2139 2972 2993 3135 3408 3725 4159 5048 6677 Size: 833 21 142 273 317 434 889 1629 Cuts at: 6677 8 Size: 43 Fragments arranged by size: 1629 1425 889 833 434 317 275 273 203 142 58 54 50 48 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2033 2081 2139 2993 3725 4159 1537 Size: 275 221 48 58 854 732 434 4090 Fragments arranged by size: 4090 854 732 434 275 221 58 48 EarI CTCTTCn'nnn_ Cuts at: 1353 2298 2768 4642 1353 Size: 945 470 1874 3423 Fragments arranged by size: 3423 1874 945 470 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2218 3779 5299 5673 5819 387 Size: 1064 138 629 1561 1520 374 146 1280 Fragments arranged by size: 1561 1520 1280 1064 629 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2719 2797 2825 3362 4775 5372 6159 Size: 393 1087 78 28 537 1413 597 787 Cuts at: 6159 6427 1239 Size: 268 1524 Fragments arranged by size: 1524 1413 1087 787 597 537 393 268 78 28 EcoO109I rG'GnC_Cy Cuts at: 2502 3056 3552 3701 2502 Size: 554 496 149 5513 Fragments arranged by size: 5513 554 496 149 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FseI GG_CCGG'CC Cuts at: 2983 2983 Size: 6712 FspI TGC'GCA Cuts at: 2498 5218 2498 Size: 2720 3992 HaeII r_GCGC'y Cuts at: 1678 2269 2398 2840 3280 3469 3628 3994 5859 Size: 591 129 442 440 189 159 366 1865 Cuts at: 5859 1678 Size: 2531 Fragments arranged by size: 2531 1865 591 442 440 366 189 159 129 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5353 5385 5427 5459 1373 Size: 32 135 32 3781 32 42 32 2626 Fragments arranged by size: 3781 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2863 3820 4056 4302 669 Size: 988 281 925 957 236 246 3079 Fragments arranged by size: 3079 988 957 925 281 246 236 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KasI G'GCGC_C Cuts at: 2836 2836 Size: 6712 KpnI G_GTAC'C Cuts at: 2479 2919 3505 4037 2479 Size: 440 586 532 5154 Fragments arranged by size: 5154 586 532 440 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2369 5826 6010 1305 Size: 1064 3457 184 2007 Fragments arranged by size: 3457 2007 1064 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3137 10 Size: 54 1425 343 1305 3585 Fragments arranged by size: 3585 1425 1305 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 3747 4670 5029 5188 518 Size: 648 1125 1456 923 359 159 2042 Fragments arranged by size: 2042 1456 1125 923 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2334 3174 3364 4128 4805 5953 6198 Size: 492 236 840 190 764 677 1148 245 Cuts at: 6198 1606 Size: 2120 Fragments arranged by size: 2120 1148 840 764 677 492 245 236 190 NaeI GCC'GGC Cuts at: 2981 2981 Size: 6712 NarI GG'CG_CC Cuts at: 2837 2837 Size: 6712 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5221 Fragments arranged by size: 5221 939 552 NdeI CA'TA_TG Cuts at: 387 2211 387 Size: 1824 4888 NgoMIV G'CCGG_C Cuts at: 2979 2979 Size: 6712 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 2081 4159 2081 Size: 2078 4634 NspI r_CATG'y Cuts at: 1132 1229 4070 5615 1132 Size: 97 2841 1545 2229 Fragments arranged by size: 2841 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3497 1348 Size: 2149 4563 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2316 2485 2797 3417 3606 1108 Size: 1131 77 169 312 620 189 4214 Fragments arranged by size: 4214 1131 620 312 189 169 77 PfoI T'CCnGG_A Cuts at: 2541 3204 2541 Size: 663 6049 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PmlI CAC'GTG Cuts at: 3691 3971 3691 Size: 280 6432 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6712 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspOMI G'GGCC_C Cuts at: 3055 3055 Size: 6712 PstI C_TGCA'G Cuts at: 830 4002 4064 830 Size: 3172 62 3478 Fragments arranged by size: 3478 3172 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SfcI C'TryA_G Cuts at: 826 1045 3998 4060 5195 5876 6067 826 Size: 219 2953 62 1135 681 191 1471 Fragments arranged by size: 2953 1471 1135 681 219 191 62 SfiI GGCCn_nnn'nGGCC Cuts at: 2980 3001 2980 Size: 21 6691 SfoI GGC'GCC Cuts at: 2838 2838 Size: 6712 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 2288 2648 1700 Size: 588 360 5764 Fragments arranged by size: 5764 588 360 SmaI CCC'GGG Cuts at: 1872 3760 4039 1872 Size: 1888 279 4545 Fragments arranged by size: 4545 1888 279 SmlI C'TyrA_G Cuts at: 820 1017 1943 4816 5717 5979 820 Size: 197 926 2873 901 262 1553 Fragments arranged by size: 2873 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4503 493 Size: 4010 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 4070 1132 Size: 2938 3774 SspI AAT'ATT Cuts at: 5 52 4636 5 Size: 47 4584 2081 Fragments arranged by size: 4584 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6712 StyI C'CwwG_G Cuts at: 513 1065 2004 2128 2310 2689 4151 513 Size: 552 939 124 182 379 1462 3074 Fragments arranged by size: 3074 1462 939 552 379 182 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 4739 4756 4909 5094 5539 325 Size: 1255 1137 2022 17 153 185 445 1498 Fragments arranged by size: 2022 1498 1255 1137 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4958 6670 96 Size: 274 80 33 51 1443 2981 1712 138 Fragments arranged by size: 2981 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6672 6704 6672 Size: 32 6680 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6712 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2830 3376 3705 2830 Size: 546 329 5837 Fragments arranged by size: 5837 546 329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 3758 4037 1870 Size: 1888 279 4545 Fragments arranged by size: 4545 1888 279 XmnI GAAnn'nnTTC Cuts at: 4841 4841 Size: 6712 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI