(Circular) (Six-base) MAPSORT of: pFN21AB6289.seq Check: 693 from: 1 to: 6994 pFN21AB6289 6994 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2903 4101 4804 278 Size: 53 83 186 936 1367 1198 703 2468 Fragments arranged by size: 2468 1367 1198 936 703 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 1937 Size: 2400 4594 Acc65I G'GTAC_C Cuts at: 3139 4315 3139 Size: 1176 5818 AclI AA'CG_TT Cuts at: 1563 5121 5494 1563 Size: 3558 373 3063 Fragments arranged by size: 3558 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5057 6441 6709 1632 Size: 3425 1384 268 1917 Fragments arranged by size: 3425 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6797 AflIII A'CryG_T Cuts at: 1203 2883 5893 6870 1203 Size: 1680 3010 977 1327 Fragments arranged by size: 3010 1680 1327 977 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 2992 5723 2992 Size: 2731 4263 AleI CACnn'nnGTG Cuts at: 1166 2940 1166 Size: 1774 5220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2542 4223 1184 Size: 1358 1681 3955 Fragments arranged by size: 3955 1681 1358 AlwNI CAG_nnn'CTG Cuts at: 3169 4737 6309 3169 Size: 1568 1572 3854 Fragments arranged by size: 3854 1572 1568 ApaI G_GGCC'C Cuts at: 2313 3702 4253 2313 Size: 1389 551 5054 Fragments arranged by size: 5054 1389 551 ApaLI G'TGCA_C Cuts at: 5051 6207 5051 Size: 1156 5838 ApoI r'AATT_y Cuts at: 1493 2318 4303 4529 4681 6790 1493 Size: 825 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 825 226 152 AseI AT'TA_AT Cuts at: 160 1032 5548 160 Size: 872 4516 1606 Fragments arranged by size: 4516 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 3008 3838 4319 1870 Size: 73 1065 830 481 4545 Fragments arranged by size: 4545 1065 830 481 73 BamHI G'GATC_C Cuts at: 4324 4691 4324 Size: 367 6627 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3139 3193 3938 4315 5770 Size: 325 217 627 1352 54 745 377 1455 Cuts at: 5770 618 Size: 1842 Fragments arranged by size: 1842 1455 1352 745 627 377 325 217 54 BanII G_rGCy'C Cuts at: 1391 2313 2347 2479 2594 3051 3227 3449 3702 Size: 922 34 132 115 457 176 222 253 Cuts at: 3702 3729 4253 4313 1391 Size: 27 524 60 4072 Fragments arranged by size: 4072 922 524 457 253 222 176 132 115 60 34 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6023 BbvCI CC'TCA_GC Cuts at: 3282 4196 3282 Size: 914 6080 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5185 5219 1903 Size: 34 3248 34 3678 Fragments arranged by size: 3678 3248 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4886 6096 4886 Size: 1210 5784 BclI T'GATC_A Cuts at: 1548 1548 Size: 6994 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5605 136 Size: 107 122 71 631 605 3933 1525 Fragments arranged by size: 3933 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6989 6989 Size: 6994 BlpI GC'TnA_GC Cuts at: 3767 4411 3767 Size: 644 6350 Bme1580I G_kGCm'C Cuts at: 1790 2313 3702 4253 5055 6211 1790 Size: 523 1389 551 802 1156 2573 Fragments arranged by size: 2573 1389 1156 802 551 523 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6994 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2597 2934 3696 Size: 416 53 83 399 24 1180 337 762 Cuts at: 3696 3914 4256 4267 4725 5683 442 Size: 218 342 11 458 958 1753 Fragments arranged by size: 1753 1180 958 762 458 416 399 342 337 218 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 3281 1056 Size: 2225 4769 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 3484 3651 2182 Size: 1302 167 5525 Fragments arranged by size: 5525 1302 167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 2438 2493 2660 3895 5654 1239 Size: 1104 95 55 167 1235 1759 2579 Fragments arranged by size: 2579 1759 1235 1104 167 95 55 Bpu10I CC'TnA_GC Cuts at: 3282 3563 4196 4284 3282 Size: 281 633 88 5992 Fragments arranged by size: 5992 633 281 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5119 5984 6282 5119 Size: 865 298 5831 Fragments arranged by size: 5831 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 2492 2668 2923 3000 3421 3639 Size: 617 915 78 176 255 77 421 218 Cuts at: 3639 5657 882 Size: 2018 2219 Fragments arranged by size: 2219 2018 915 617 421 255 218 176 78 77 BsaAI yAC'GTr Cuts at: 493 1204 2796 4785 493 Size: 711 1592 1989 2702 Fragments arranged by size: 2702 1989 1592 711 BsaBI GATnn'nnATC Cuts at: 2454 6988 2454 Size: 4534 2460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2900 4098 4801 5183 Size: 53 83 186 936 1367 1198 703 382 Cuts at: 5183 5818 275 Size: 635 1451 Fragments arranged by size: 1451 1367 1198 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5427 5825 6099 6246 6657 5427 Size: 398 274 147 411 5764 Fragments arranged by size: 5764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2341 2371 3503 3533 3743 Size: 30 165 30 938 30 1132 30 210 Cuts at: 3743 3773 4043 4073 1178 Size: 30 270 30 4099 Fragments arranged by size: 4099 1132 938 270 210 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2678 3288 3299 3341 3631 3640 3736 Size: 919 573 610 11 42 290 9 96 Cuts at: 3736 3820 4022 1186 Size: 84 202 4158 Fragments arranged by size: 4158 919 610 573 290 202 96 84 42 11 9 BseYI C'CCAG_C Cuts at: 2183 3714 3736 3762 3889 6197 6693 2183 Size: 1531 22 26 127 2308 496 2484 Fragments arranged by size: 2484 2308 1531 496 127 26 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2512 2526 2861 1163 Size: 573 776 14 335 5296 Fragments arranged by size: 5296 776 573 335 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3038 4444 5205 6233 1540 Size: 275 186 1037 1406 761 1028 2301 Fragments arranged by size: 2301 1406 1037 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3015 3051 3227 3729 4006 5055 5140 6211 3015 Size: 36 176 502 277 1049 85 1071 3798 Fragments arranged by size: 3798 1071 1049 502 277 176 85 36 BsmI GAATG_Cn' Cuts at: 4505 4598 4505 Size: 93 6901 BsmBI CGTCTCn'nnnn_ Cuts at: 2891 2891 Size: 6994 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2313 2347 2479 2594 3015 3051 3227 Size: 399 523 34 132 115 421 36 176 Cuts at: 3227 3449 3702 3729 4006 4253 4313 5055 5140 Size: 222 253 27 277 247 60 742 85 Cuts at: 5140 6211 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 523 421 399 277 253 247 222 176 132 115 85 60 36 34 27 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 3219 4883 6613 3219 Size: 1664 1730 3600 Fragments arranged by size: 3600 1730 1664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 4349 844 Size: 1602 1903 3489 Fragments arranged by size: 3489 1903 1602 BsrBI CCG'CTC Cuts at: 1394 1823 2710 4881 1394 Size: 429 887 2171 3507 Fragments arranged by size: 3507 2171 887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2829 3197 3258 5489 5663 65 Size: 1183 1581 368 61 2231 174 1396 Fragments arranged by size: 2231 1581 1396 1183 368 174 61 BsrFI r'CCGG_y Cuts at: 1700 1810 3630 5638 5825 1700 Size: 110 1820 2008 187 2869 Fragments arranged by size: 2869 2008 1820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6994 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 5054 6066 5054 Size: 1012 5982 BstAPI GCAn_nnn'nTGC Cuts at: 794 2279 3655 794 Size: 1485 1376 4133 Fragments arranged by size: 4133 1485 1376 BstBI TT'CG_AA Cuts at: 2566 5798 2566 Size: 3232 3762 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6994 BstXI CCAn_nnnn'nTGG Cuts at: 2152 3439 2152 Size: 1287 5707 BstYI r'GATC_y Cuts at: 1972 2105 3976 4203 4324 4691 5076 5093 6545 Size: 133 1871 227 121 367 385 17 1452 Cuts at: 6545 6631 6643 6989 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1871 1452 385 367 346 227 133 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3119 513 Size: 552 2054 4388 Fragments arranged by size: 4388 2054 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6255 Fragments arranged by size: 6255 683 56 BtsI GCAGTG_nn' Cuts at: 3110 4510 5304 5324 3110 Size: 1400 794 20 4780 Fragments arranged by size: 4780 1400 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6959 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2888 6001 809 Size: 507 1572 3113 1802 Fragments arranged by size: 3113 1802 1572 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 2232 2711 Size: 54 1425 50 275 18 194 208 479 Cuts at: 2711 2730 4441 5330 6959 8 Size: 19 1711 889 1629 43 Fragments arranged by size: 1711 1629 1425 889 479 275 208 194 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3453 3692 4084 4924 1353 Size: 2100 239 392 840 3423 Fragments arranged by size: 3423 2100 840 392 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2849 3041 3112 5581 5955 6101 Size: 1064 138 1260 192 71 2469 374 146 Cuts at: 6101 387 Size: 1280 Fragments arranged by size: 2469 1280 1260 1064 374 192 146 138 71 EcoICRI GAG'CTC Cuts at: 3049 3225 3727 3049 Size: 176 502 6316 Fragments arranged by size: 6316 502 176 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2438 2493 2660 3895 5057 5654 Size: 393 711 95 55 167 1235 1162 597 Cuts at: 5654 6441 6709 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1235 1162 787 711 597 393 268 167 95 55 EcoNI CCTnn'n_nnAGG Cuts at: 4235 4235 Size: 6994 EcoO109I rG'GnC_Cy Cuts at: 3567 3698 3791 4240 4250 3567 Size: 131 93 449 10 6311 Fragments arranged by size: 6311 449 131 93 10 EcoRI G'AATT_C Cuts at: 4303 4681 4303 Size: 378 6616 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2938 2938 Size: 6994 FspI TGC'GCA Cuts at: 5500 5500 Size: 6994 HaeII r_GCGC'y Cuts at: 1678 6141 1678 Size: 4463 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2978 3010 3283 3315 3482 Size: 32 135 32 1406 32 273 32 167 Cuts at: 3482 3503 3514 3535 3743 3775 4043 4075 5635 Size: 21 11 21 208 32 268 32 1560 Cuts at: 5635 5667 5709 5741 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1560 1406 273 268 208 167 135 42 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 4338 4584 669 Size: 988 281 2400 246 3079 Fragments arranged by size: 3079 2400 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6994 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KpnI G_GTAC'C Cuts at: 3143 4319 3143 Size: 1176 5818 MfeI C'AATT_G Cuts at: 3002 4593 3002 Size: 1591 5403 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 2574 6108 6292 1305 Size: 882 387 3534 184 2007 Fragments arranged by size: 3534 2007 882 387 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2732 10 Size: 54 1425 343 402 498 4272 Fragments arranged by size: 4272 1425 498 402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 2940 3437 4952 5311 5470 518 Size: 648 1566 208 497 1515 359 159 2042 Fragments arranged by size: 2042 1566 1515 648 497 359 208 159 MspA1I CmG'CkG Cuts at: 1606 2183 2334 3714 4410 5087 6235 6480 1606 Size: 577 151 1380 696 677 1148 245 2120 Fragments arranged by size: 2120 1380 1148 696 677 577 245 151 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6442 NdeI CA'TA_TG Cuts at: 387 387 Size: 6994 NheI G'CTAG_C Cuts at: 1052 3277 1052 Size: 2225 4769 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NspI r_CATG'y Cuts at: 1132 1229 2887 4352 5897 1132 Size: 97 1658 1465 1545 2229 Fragments arranged by size: 2229 1658 1545 1465 97 PasI CC'CwG_GG Cuts at: 1348 2990 3085 3934 3964 1348 Size: 1642 95 849 30 4378 Fragments arranged by size: 4378 1642 849 95 30 PciI A'CATG_T Cuts at: 2883 5893 2883 Size: 3010 3984 PflMI CCAn_nnn'nTGG Cuts at: 1108 3169 3784 1108 Size: 2061 615 4318 Fragments arranged by size: 4318 2061 615 PfoI T'CCnGG_A Cuts at: 2359 2892 3872 4173 2359 Size: 533 980 301 5180 Fragments arranged by size: 5180 980 533 301 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5063 5095 6576 6608 3716 Size: 32 1315 32 1481 32 4102 Fragments arranged by size: 4102 1481 1315 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3567 3791 4240 3567 Size: 224 449 6321 Fragments arranged by size: 6321 449 224 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6994 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PspOMI G'GGCC_C Cuts at: 2309 3698 4249 2309 Size: 1389 551 5054 Fragments arranged by size: 5054 1389 551 PstI C_TGCA'G Cuts at: 830 2443 3313 4346 830 Size: 1613 870 1033 3478 Fragments arranged by size: 3478 1613 1033 870 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 2183 2334 2183 Size: 151 6843 SacI G_AGCT'C Cuts at: 3051 3227 3729 3051 Size: 176 502 6316 Fragments arranged by size: 6316 502 176 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SapI GCTCTTCn'nnn_ Cuts at: 3692 3692 Size: 6994 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 3706 5242 6954 3706 Size: 1536 1712 3746 Fragments arranged by size: 3746 1712 1536 SexAI A'CCwGG_T Cuts at: 2967 3135 2967 Size: 168 6826 SfcI C'TryA_G Cuts at: 826 1045 2439 3309 3553 4289 4342 5477 6158 Size: 219 1394 870 244 736 53 1135 681 Cuts at: 6158 6349 826 Size: 191 1471 Fragments arranged by size: 1471 1394 1135 870 736 681 244 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 4321 1872 Size: 2449 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5098 5999 6261 820 Size: 197 926 3155 901 262 1553 Fragments arranged by size: 3155 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6994 SphI G_CATG'C Cuts at: 1132 4352 1132 Size: 3220 3774 SspI AAT'ATT Cuts at: 5 52 2254 4918 5 Size: 47 2202 2664 2081 Fragments arranged by size: 2664 2202 2081 47 StuI AGG'CCT Cuts at: 1517 2141 4167 1517 Size: 624 2026 4344 Fragments arranged by size: 4344 2026 624 StyI C'CwwG_G Cuts at: 513 1065 2145 2283 2424 3267 3453 4433 513 Size: 552 1080 138 141 843 186 980 3074 Fragments arranged by size: 3074 1080 980 843 552 186 141 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3052 3820 5021 5038 5191 5376 5821 Size: 1255 1472 768 1201 17 153 185 445 Cuts at: 5821 325 Size: 1498 Fragments arranged by size: 1498 1472 1255 1201 768 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 3922 5240 Size: 274 80 33 51 1443 1727 218 1318 Cuts at: 5240 6952 96 Size: 1712 138 Fragments arranged by size: 1727 1712 1443 1318 274 218 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2855 2887 6954 6986 2855 Size: 32 4067 32 2863 Fragments arranged by size: 4067 2863 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2685 2685 Size: 6994 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 4319 1870 Size: 2449 4545 XmnI GAAnn'nnTTC Cuts at: 5123 5123 Size: 6994 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2901 4099 4802 276 Size: 53 83 186 936 1367 1198 703 2468 Fragments arranged by size: 2468 1367 1198 936 703 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI