Restriction map for
pFN21AB6752
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I BspDI BspEI BspHI
BsrBI BsrDI BsrI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 286 GTCTAC GT^[AC][GT]AC
AfaI 108 GTAC GT^AC
AflIII 80 ACATGT A^C[GA][CT]GT
AlwNI 70 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 179 TGCGCA TGC^GCA
BanI 198 GGCGCC G^G[CT][GA]CC
BbeI 198 GGCGCC GGCGC^C
BbsI 228 GAAGAC GAAGAC(2/6)
BclI 248 TGATCA T^GATCA
BglI 386 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 228 GAAGAC GAAGAC(2/6)
BseRI 411 CTCCTC GAGGAG(10/8)
BsmFI 242 GGGAC GGGAC(10/14)
Bsp1407I 107 TGTACA T^GTACA
BspLU11I 80 ACATGT A^CATGT
BspMI 236 ACCTGC ACCTGC(4/8)
BsrFI 386 GCCGGC [GA]^CCGG[CT]
BsrGI 107 TGTACA T^GTACA
BssHII 115 GCGCGC G^CGCGC
Cfr10I 386 GCCGGC [GA]^CCGG[CT]
DraII 140 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 177 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 140 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 206 CCATGG C^C[AT][AT]GG
FbaI 248 TGATCA T^GATCA
FseI 385 GGCCGGCC GGCCGG^CC
FspI 179 TGCGCA TGC^GCA
KasI 198 GGCGCC G^GCGCC
KspI 51 CCGCGG CCGC^GG
NaeI 386 GCCGGC GCC^GGC
NarI 198 GGCGCC GG^CGCC
NcoI 206 CCATGG C^CATGG
NgoMIV 386 GCCGGC G^CCGGC
NspI 80 ACATGT [GA]CATG^[CT]
RsaI 108 GTAC GT^AC
SacII 51 CCGCGG CCGC^GG
SexAI 308 ACCAGGT A^CC[AT]GGT
SfoI 198 GGCGCC GGC^GCC
SmlI 430 CTCAAG C^T[CT][GA]AG
SspBI 107 TGTACA T^GTACA
StyI 206 CCATGG C^C[AT][AT]GG
TaqI 65 TCGA T^CGA
TspRI 111 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 65 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 52 CGCG CG^CG
116 CGCG
AcyI 198 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
AvaI 259 CCCGAG C^[CT]CG[GA]G
325 CCCGAG
AvaII 29 GGACC G^G[AT]CC
60 GGACC
BcnI 138 CCGGG CC^[GC]GG
391 CCCGG
BsaHI 198 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
BsoBI 259 CCCGAG C^[CT]CG[GA]G
325 CCCGAG
BstAPI 93 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
159 GCACTCCCTGC
BstF5I 252 CATCC GGATG(2/0)
423 CATCC
BstNI 309 CCAGG CC^[AT]GG
345 CCTGG
BstUI 52 CGCG CG^CG
116 CGCG
DsaI 51 CCGCGG C^C[GA][CT]GG
206 CCATGG
EaeI 336 CGGCCA [CT]^GGCC[GA]
384 TGGCCG
EcoRII 309 CCAGG ^CC[AT]GG
345 CCTGG
FauI 416 CCCGC CCCGC(4/6)
240 GCGGG
FokI 252 CATCC GGATG(9/13)
423 CATCC
HaeII 173 AGCGCC [GA]GCGC^[CT]
198 GGCGCC
HgaI 231 GACGC GACGC(5/10)
284 GCGTC
Hin1I 198 GGCGCC G[GA]^CG[CT]C
283 GGCGTC
MvaI 309 CCAGG CC^[AT]GG
345 CCTGG
MvnI 52 CGCG CG^CG
116 CGCG
NciI 138 CCGGG CC^[GC]GG
391 CCCGG
PflMI 69 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCACTACCTGG
SfaNI 99 GATGC GCATC(5/9)
395 GCATC
Van91I 69 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
339 CCACTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI Cac8I CfoI Cfr13I
DpnII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TseI