(Circular) (Six-base) MAPSORT of: pFN21AB6227.seq Check: 109 from: 1 to: 6721 pFN21AB6227 6721 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2414 2414 Size: 6721 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4531 278 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4064 1937 Size: 2127 4594 Acc65I G'GTAC_C Cuts at: 4042 4042 Size: 6721 AclI AA'CG_TT Cuts at: 1563 2418 3666 4848 5221 1563 Size: 855 1248 1182 373 3063 Fragments arranged by size: 3063 1248 1182 855 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 2988 3134 3416 4784 6168 6436 1632 Size: 1056 300 146 282 1368 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1056 300 282 268 146 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6524 AflIII A'CryG_T Cuts at: 1203 5620 6597 1203 Size: 4417 977 1327 Fragments arranged by size: 4417 1327 977 AgeI A'CCGG_T Cuts at: 5552 5552 Size: 6721 AhdI GACnn_n'nnGTC Cuts at: 3908 5450 3908 Size: 1542 5179 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6721 AlwNI CAG_nnn'CTG Cuts at: 2160 2454 4464 6036 2160 Size: 294 2010 1572 2845 Fragments arranged by size: 2845 2010 1572 294 ApaLI G'TGCA_C Cuts at: 2096 3151 3995 4778 5934 2096 Size: 1055 844 783 1156 2883 Fragments arranged by size: 2883 1156 1055 844 783 ApoI r'AATT_y Cuts at: 1493 2117 2243 2915 3305 4030 4256 4408 6517 Size: 624 126 672 390 725 226 152 2109 Cuts at: 6517 1493 Size: 1697 Fragments arranged by size: 2109 1697 725 672 624 390 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 5275 160 Size: 872 4243 1606 Fragments arranged by size: 4243 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 AvaI C'yCGr_G Cuts at: 1870 1943 2020 4046 1870 Size: 73 77 2026 4545 Fragments arranged by size: 4545 2026 77 73 BamHI G'GATC_C Cuts at: 2563 2774 4051 4418 2563 Size: 211 1277 367 4866 Fragments arranged by size: 4866 1277 367 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 4042 5497 618 Size: 325 217 627 448 1807 1455 1842 Fragments arranged by size: 1842 1807 1455 627 448 325 217 BanII G_rGCy'C Cuts at: 1391 4040 1391 Size: 2649 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2976 3639 928 Size: 971 1077 663 4010 Fragments arranged by size: 4010 1077 971 663 BbvCI CC'TCA_GC Cuts at: 2081 2502 2081 Size: 421 6300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4912 4946 1903 Size: 34 2975 34 3678 Fragments arranged by size: 3678 2975 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5823 4613 Size: 1210 5511 BclI T'GATC_A Cuts at: 1548 3925 1548 Size: 2377 4344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2943 5332 136 Size: 107 122 71 631 605 1271 2389 1525 Fragments arranged by size: 2389 1525 1271 631 605 122 107 71 BglII A'GATC_T Cuts at: 6716 6716 Size: 6721 BlpI GC'TnA_GC Cuts at: 4138 4138 Size: 6721 Bme1580I G_kGCm'C Cuts at: 1790 2100 3086 3155 3999 4782 5938 1790 Size: 310 986 69 844 783 1156 2573 Fragments arranged by size: 2573 1156 986 844 783 310 69 BmgBI CAC'GTC Cuts at: 1318 2050 3145 3775 1318 Size: 732 1095 630 4264 Fragments arranged by size: 4264 1095 732 630 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2752 3817 3982 Size: 416 53 83 399 24 1335 1065 165 Cuts at: 3982 4452 5410 442 Size: 470 958 1753 Fragments arranged by size: 1753 1335 1065 958 470 416 399 165 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6721 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3026 3026 Size: 6721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3492 3839 5381 1239 Size: 2253 347 1542 2579 Fragments arranged by size: 2579 2253 1542 347 Bpu10I CC'TnA_GC Cuts at: 2081 2502 2081 Size: 421 6300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4846 5711 6009 4846 Size: 865 298 5558 Fragments arranged by size: 5558 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5384 882 Size: 617 3885 2219 Fragments arranged by size: 3885 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4512 493 Size: 711 3308 2702 Fragments arranged by size: 3308 2702 711 BsaBI GATnn'nnATC Cuts at: 3347 6715 3347 Size: 3368 3353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2039 3579 4528 4910 Size: 53 83 186 936 506 1540 949 382 Cuts at: 4910 5545 275 Size: 635 1451 Fragments arranged by size: 1540 1451 949 936 635 506 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2875 3795 5154 5552 5826 5973 6384 2875 Size: 920 1359 398 274 147 411 3212 Fragments arranged by size: 3212 1359 920 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6496 Fragments arranged by size: 6496 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6721 BseYI C'CCAG_C Cuts at: 2447 3733 3745 5924 6420 2447 Size: 1286 12 2179 496 2748 Fragments arranged by size: 2748 2179 1286 496 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 3197 4035 1163 Size: 573 410 1051 838 3849 Fragments arranged by size: 3849 1051 838 573 410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4171 4932 5960 1540 Size: 275 186 2170 761 1028 2301 Fragments arranged by size: 2301 2170 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 3155 3663 3999 4782 4867 5938 2100 Size: 1055 508 336 783 85 1071 2883 Fragments arranged by size: 2883 1071 1055 783 508 336 85 BsmI GAATG_Cn' Cuts at: 4232 4325 4232 Size: 93 6628 BsmBI CGTCTCn'nnnn_ Cuts at: 2046 2046 Size: 6721 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 3086 3155 3663 3999 4040 4782 Size: 399 310 986 69 508 336 41 742 Cuts at: 4782 4867 5938 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 986 742 508 399 336 310 85 69 41 BspEI T'CCGG_A Cuts at: 2875 3795 6384 2875 Size: 920 2589 3212 Fragments arranged by size: 3212 2589 920 BspHI T'CATG_A Cuts at: 4610 6340 4610 Size: 1730 4991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 4076 844 Size: 1570 1662 3489 Fragments arranged by size: 3489 1662 1570 BsrBI CCG'CTC Cuts at: 1394 1823 2652 4608 1394 Size: 429 829 1956 3507 Fragments arranged by size: 3507 1956 829 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2435 5216 5390 65 Size: 1183 1187 2781 174 1396 Fragments arranged by size: 2781 1396 1187 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2812 5365 5552 1700 Size: 110 1002 2553 187 2869 Fragments arranged by size: 2869 2553 1002 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6721 BssHII G'CGCG_C Cuts at: 1925 2126 1925 Size: 201 6520 BssSI C'ACGA_G Cuts at: 2099 3897 4781 5793 2099 Size: 1798 884 1012 3027 Fragments arranged by size: 3027 1798 1012 884 BstAPI GCAn_nnn'nTGC Cuts at: 794 2160 794 Size: 1366 5355 BstBI TT'CG_AA Cuts at: 5525 5525 Size: 6721 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6721 BstXI CCAn_nnnn'nTGG Cuts at: 3745 3745 Size: 6721 BstYI r'GATC_y Cuts at: 1972 2563 2581 2774 3391 3831 4051 4418 4803 Size: 591 18 193 617 440 220 367 385 Cuts at: 4803 4820 6272 6358 6370 6716 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 617 591 440 385 367 346 220 193 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 3759 3759 Size: 6721 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 3156 513 Size: 552 939 30 1122 4078 Fragments arranged by size: 4078 1122 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2107 487 Size: 683 56 881 5101 Fragments arranged by size: 5101 881 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2363 2675 4237 5031 5051 2158 Size: 205 312 1562 794 20 3828 Fragments arranged by size: 3828 1562 794 312 205 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6721 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3841 3876 529 Size: 35 3277 35 3374 Fragments arranged by size: 3374 3277 35 35 DraI TTT'AAA Cuts at: 4025 4025 Size: 6721 DraIII CAC_nnn'GTG Cuts at: 2104 2104 Size: 6721 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5728 809 Size: 507 4412 1802 Fragments arranged by size: 4412 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2814 4168 Size: 54 1425 50 275 18 222 762 1354 Cuts at: 4168 5057 6686 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1354 889 762 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4168 1537 Size: 275 2356 4090 Fragments arranged by size: 4090 2356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 4651 1353 Size: 1118 2180 3423 Fragments arranged by size: 3423 2180 1118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2500 5308 5682 5828 387 Size: 1064 138 911 2808 374 146 1280 Fragments arranged by size: 2808 1280 1064 911 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 2988 3134 3416 3492 3839 4784 Size: 393 1056 300 146 282 76 347 945 Cuts at: 4784 5381 6168 6436 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1056 945 787 597 393 347 300 282 268 146 76 EcoNI CCTnn'n_nnAGG Cuts at: 3116 3116 Size: 6721 EcoO109I rG'GnC_Cy Cuts at: 2279 3274 3492 2279 Size: 995 218 5508 Fragments arranged by size: 5508 995 218 EcoRI G'AATT_C Cuts at: 4030 4408 4030 Size: 378 6343 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2948 3190 2948 Size: 242 6479 FspI TGC'GCA Cuts at: 5227 5227 Size: 6721 HaeII r_GCGC'y Cuts at: 1678 2081 5868 1678 Size: 403 3787 2531 Fragments arranged by size: 3787 2531 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3220 3252 3894 3926 5362 Size: 32 135 32 1648 32 642 32 1436 Cuts at: 5362 5394 5436 5468 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1648 1436 642 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3265 3982 4065 4311 669 Size: 988 281 1327 717 83 246 3079 Fragments arranged by size: 3079 1327 988 717 281 246 83 HindIII A'AGCT_T Cuts at: 748 748 Size: 6721 HpaI GTT'AAC Cuts at: 4311 4311 Size: 6721 KpnI G_GTAC'C Cuts at: 4046 4046 Size: 6721 MfeI C'AATT_G Cuts at: 4320 4320 Size: 6721 MluI A'CGCG_T Cuts at: 6597 6597 Size: 6721 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3012 3396 5835 6019 1305 Size: 1707 384 2439 184 2007 Fragments arranged by size: 2439 2007 1707 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2054 10 Size: 54 1425 343 222 4677 Fragments arranged by size: 4677 1425 343 222 54 MslI CAynn'nnrTG Cuts at: 518 1166 4679 5038 5197 518 Size: 648 3513 359 159 2042 Fragments arranged by size: 3513 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3211 3931 4137 4814 5962 6207 1606 Size: 1605 720 206 677 1148 245 2120 Fragments arranged by size: 2120 1605 1148 720 677 245 206 NaeI GCC'GGC Cuts at: 2814 2814 Size: 6721 NcoI C'CATG_G Cuts at: 513 1065 2004 3156 513 Size: 552 939 1152 4078 Fragments arranged by size: 4078 1152 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6721 NgoMIV G'CCGG_C Cuts at: 2812 2812 Size: 6721 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6721 NotI GC'GGCC_GC Cuts at: 4168 4168 Size: 6721 NspI r_CATG'y Cuts at: 1132 1229 3788 4079 5624 1132 Size: 97 2559 291 1545 2229 Fragments arranged by size: 2559 2229 1545 291 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6721 PciI A'CATG_T Cuts at: 5620 5620 Size: 6721 PflMI CCAn_nnn'nTGG Cuts at: 1108 2823 3232 3620 1108 Size: 1715 409 388 4209 Fragments arranged by size: 4209 1715 409 388 PmeI GTTT'AAAC Cuts at: 4025 4025 Size: 6721 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4790 4822 6303 6335 4790 Size: 32 1481 32 5176 Fragments arranged by size: 5176 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2279 3274 3492 2279 Size: 995 218 5508 Fragments arranged by size: 5508 995 218 PshAI GACnn'nnGTC Cuts at: 1613 2722 1613 Size: 1109 5612 PsiI TTA'TAA Cuts at: 4291 4291 Size: 6721 PstI C_TGCA'G Cuts at: 830 2156 4073 830 Size: 1326 1917 3478 Fragments arranged by size: 3478 1917 1326 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6721 PvuII CAG'CTG Cuts at: 3211 3931 3211 Size: 720 6001 SalI G'TCGA_C Cuts at: 1936 4063 1936 Size: 2127 4594 SanDI GG'GwC_CC Cuts at: 2279 2279 Size: 6721 SbfI CC_TGCA'GG Cuts at: 4073 4073 Size: 6721 ScaI AGT'ACT Cuts at: 2858 4969 6681 2858 Size: 2111 1712 2898 Fragments arranged by size: 2898 2111 1712 SfcI C'TryA_G Cuts at: 826 1045 2152 2727 4069 5204 5885 6076 826 Size: 219 1107 575 1342 1135 681 191 1471 Fragments arranged by size: 1471 1342 1135 1107 681 575 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6721 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6721 SmaI CCC'GGG Cuts at: 1872 2022 4048 1872 Size: 150 2026 4545 Fragments arranged by size: 4545 2026 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 4825 5726 5988 820 Size: 197 926 2882 901 262 1553 Fragments arranged by size: 2882 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4512 493 Size: 4019 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6721 SphI G_CATG'C Cuts at: 1132 3788 4079 1132 Size: 2656 291 3774 Fragments arranged by size: 3774 2656 291 SspI AAT'ATT Cuts at: 5 52 4645 5 Size: 47 4593 2081 Fragments arranged by size: 4593 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6721 StyI C'CwwG_G Cuts at: 513 1065 2004 3156 3690 4160 513 Size: 552 939 1152 534 470 3074 Fragments arranged by size: 3074 1152 939 552 534 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2730 3015 3747 4748 4765 4918 5103 Size: 1255 1150 285 732 1001 17 153 185 Cuts at: 5103 5548 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1150 1001 732 445 285 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 3243 4967 Size: 274 80 33 51 1443 879 387 1724 Cuts at: 4967 6679 96 Size: 1712 138 Fragments arranged by size: 1724 1712 1443 879 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2383 2415 2647 2679 3484 3516 3543 3575 3653 Size: 32 232 32 805 32 27 32 78 Cuts at: 3653 3685 6681 6713 2383 Size: 32 2996 32 2391 Fragments arranged by size: 2996 2391 805 232 78 32 32 32 32 32 32 27 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6721 XbaI T'CTAG_A Cuts at: 4057 4057 Size: 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6721 XmaI C'CCGG_G Cuts at: 1870 2020 4046 1870 Size: 150 2026 4545 Fragments arranged by size: 4545 2026 150 XmnI GAAnn'nnTTC Cuts at: 2765 4850 2765 Size: 2085 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4529 276 Size: 53 83 186 936 2995 2468 Fragments arranged by size: 2995 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI