Restriction map for
pFN21AB6227
(Linear) (Six-base) MAPSORT of: KIBB6227.seq Check: 5240 from: 1 to: 2016
KIBB6227.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 409 2016
Size: 409 1607
AclI AA'CG_TT
Cuts at: 0 413 1661 2016
Size: 413 1248 355
Fragments arranged by size:
1248 413 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 983 1129 1411 2016
Size: 683 300 146 282 605
Fragments arranged by size:
683 605 300 282 146
AhdI GACnn_n'nnGTC
Cuts at: 0 1903 2016
Size: 1903 113
AlwNI CAG_nnn'CTG
Cuts at: 0 155 449 2016
Size: 155 294 1567
Fragments arranged by size:
1567 294 155
ApaLI G'TGCA_C
Cuts at: 0 91 1146 1990 2016
Size: 91 1055 844 26
Fragments arranged by size:
1055 844 91 26
ApoI r'AATT_y
Cuts at: 0 112 238 910 1300 2016
Size: 112 126 672 390 716
Fragments arranged by size:
716 672 390 126 112
AvaI C'yCGr_G
Cuts at: 0 15 2016
Size: 15 2001
BamHI G'GATC_C
Cuts at: 0 558 769 2016
Size: 558 211 1247
Fragments arranged by size:
1247 558 211
BanI G'GyrC_C
Cuts at: 0 230 2016
Size: 230 1786
BbsI GAAGACnn'nnnn_
Cuts at: 0 971 1634 2016
Size: 971 663 382
Fragments arranged by size:
971 663 382
BbvCI CC'TCA_GC
Cuts at: 0 76 497 2016
Size: 76 421 1519
Fragments arranged by size:
1519 421 76
BclI T'GATC_A
Cuts at: 0 1920 2016
Size: 1920 96
BglI GCCn_nnn'nGGC
Cuts at: 0 938 2016
Size: 938 1078
Bme1580I G_kGCm'C
Cuts at: 0 95 1081 1150 1994 2016
Size: 95 986 69 844 22
Fragments arranged by size:
986 844 95 69 22
BmgBI CAC'GTC
Cuts at: 0 45 1140 1770 2016
Size: 45 1095 630 246
Fragments arranged by size:
1095 630 246 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 747 1812 1977 2016
Size: 747 1065 165 39
Fragments arranged by size:
1065 747 165 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 2016
Size: 1021 995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 1834 2016
Size: 1487 347 182
Fragments arranged by size:
1487 347 182
Bpu10I CC'TnA_GC
Cuts at: 0 76 497 2016
Size: 76 421 1519
Fragments arranged by size:
1519 421 76
BsaBI GATnn'nnATC
Cuts at: 0 1342 2016
Size: 1342 674
BsaHI Gr'CG_yC
Cuts at: 0 34 1574 2016
Size: 34 1540 442
Fragments arranged by size:
1540 442 34
BsaWI w'CCGG_w
Cuts at: 0 870 1790 2016
Size: 870 920 226
Fragments arranged by size:
920 870 226
BseYI C'CCAG_C
Cuts at: 0 442 1728 1740 2016
Size: 442 1286 12 276
Fragments arranged by size:
1286 442 276 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1192 2016
Size: 141 1051 824
Fragments arranged by size:
1051 824 141
BsiHKAI G_wGCw'C
Cuts at: 0 95 1150 1658 1994 2016
Size: 95 1055 508 336 22
Fragments arranged by size:
1055 508 336 95 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 41 2016
Size: 41 1975
Bsp1286I G_dGCh'C
Cuts at: 0 95 1081 1150 1658 1994 2016
Size: 95 986 69 508 336 22
Fragments arranged by size:
986 508 336 95 69 22
BspEI T'CCGG_A
Cuts at: 0 870 1790 2016
Size: 870 920 226
Fragments arranged by size:
920 870 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 2016
Size: 409 1607
BsrBI CCG'CTC
Cuts at: 0 647 2016
Size: 647 1369
BsrDI GCAATG_nn'
Cuts at: 0 430 2016
Size: 430 1586
BsrFI r'CCGG_y
Cuts at: 0 807 2016
Size: 807 1209
BssHII G'CGCG_C
Cuts at: 0 121 2016
Size: 121 1895
BssSI C'ACGA_G
Cuts at: 0 94 1892 2016
Size: 94 1798 124
Fragments arranged by size:
1798 124 94
BstAPI GCAn_nnn'nTGC
Cuts at: 0 155 2016
Size: 155 1861
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1740 2016
Size: 1740 276
BstYI r'GATC_y
Cuts at: 0 558 576 769 1386 1826 2016
Size: 558 18 193 617 440 190
Fragments arranged by size:
617 558 440 193 190 18
Bsu36I CC'TnA_GG
Cuts at: 0 1754 2016
Size: 1754 262
BtgI C'CryG_G
Cuts at: 0 29 1151 2016
Size: 29 1122 865
Fragments arranged by size:
1122 865 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 102 2016
Size: 102 1914
BtsI GCAGTG_nn'
Cuts at: 0 153 358 670 2016
Size: 153 205 312 1346
Fragments arranged by size:
1346 312 205 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1836 1871 2016
Size: 1836 35 145
Fragments arranged by size:
1836 145 35
DraIII CAC_nnn'GTG
Cuts at: 0 99 2016
Size: 99 1917
EaeI y'GGCC_r
Cuts at: 0 47 809 2016
Size: 47 762 1207
Fragments arranged by size:
1207 762 47
EarI CTCTTCn'nnn_
Cuts at: 0 466 2016
Size: 466 1550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 495 2016
Size: 495 1521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 983 1129 1411 1487 1834 2016
Size: 683 300 146 282 76 347 182
Fragments arranged by size:
683 347 300 282 182 146 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1111 2016
Size: 1111 905
EcoO109I rG'GnC_Cy
Cuts at: 0 274 1269 1487 2016
Size: 274 995 218 529
Fragments arranged by size:
995 529 274 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 943 1185 2016
Size: 943 242 831
Fragments arranged by size:
943 831 242
HaeII r_GCGC'y
Cuts at: 0 76 2016
Size: 76 1940
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1215 1247 1889 1921 2016
Size: 1215 32 642 32 95
Fragments arranged by size:
1215 642 95 32 32
HincII GTy'rAC
Cuts at: 0 1260 1977 2016
Size: 1260 717 39
Fragments arranged by size:
1260 717 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1391 2016
Size: 1007 384 625
Fragments arranged by size:
1007 625 384
MscI TGG'CCA
Cuts at: 0 49 2016
Size: 49 1967
MspA1I CmG'CkG
Cuts at: 0 1206 1926 2016
Size: 1206 720 90
Fragments arranged by size:
1206 720 90
NaeI GCC'GGC
Cuts at: 0 809 2016
Size: 809 1207
NcoI C'CATG_G
Cuts at: 0 1151 2016
Size: 1151 865
NgoMIV G'CCGG_C
Cuts at: 0 807 2016
Size: 807 1209
NspI r_CATG'y
Cuts at: 0 1783 2016
Size: 1783 233
PflMI CCAn_nnn'nTGG
Cuts at: 0 818 1227 1615 2016
Size: 818 409 388 401
Fragments arranged by size:
818 409 401 388
PpuMI rG'GwC_Cy
Cuts at: 0 274 1269 1487 2016
Size: 274 995 218 529
Fragments arranged by size:
995 529 274 218
PshAI GACnn'nnGTC
Cuts at: 0 717 2016
Size: 717 1299
PstI C_TGCA'G
Cuts at: 0 151 2016
Size: 151 1865
PvuII CAG'CTG
Cuts at: 0 1206 1926 2016
Size: 1206 720 90
Fragments arranged by size:
1206 720 90
SanDI GG'GwC_CC
Cuts at: 0 274 2016
Size: 274 1742
ScaI AGT'ACT
Cuts at: 0 853 2016
Size: 853 1163
SfcI C'TryA_G
Cuts at: 0 147 722 2016
Size: 147 575 1294
Fragments arranged by size:
1294 575 147
SmaI CCC'GGG
Cuts at: 0 17 2016
Size: 17 1999
SphI G_CATG'C
Cuts at: 0 1783 2016
Size: 1783 233
StyI C'CwwG_G
Cuts at: 0 1151 1685 2016
Size: 1151 534 331
Fragments arranged by size:
1151 534 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 725 1010 1742 2016
Size: 725 285 732 274
Fragments arranged by size:
732 725 285 274
TatI w'GTAC_w
Cuts at: 0 851 1238 2016
Size: 851 387 778
Fragments arranged by size:
851 778 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 378 410 642 674 1479 1511 1538 1570
Size: 378 32 232 32 805 32 27 32
Cuts at: 1570 1648 1680 2016
Size: 78 32 336
Fragments arranged by size:
805 378 336 232 78 32 32 32
32 32 27
XmaI C'CCGG_G
Cuts at: 0 15 2016
Size: 15 2001
XmnI GAAnn'nnTTC
Cuts at: 0 760 2016
Size: 760 1256
Enzymes that do cut:
AarI AclI AcuI AhdI AlwNI ApaLI ApoI AvaI
BamHI BanI BbsI BbvCI BclI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI
MspA1I NaeI NcoI NgoMIV NspI PflMI PpuMI PshAI
PstI PvuII SanDI ScaI SfcI SmaI SphI StyI
TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BanII BbeI BcgI BciVI BfrBI BglII BlpI BmtI
BpuEI BsaI BsaAI BsaXI BseRI BsiEI BsiWI BsmI
BspHI BsrGI BstBI BstEII BstZ17I ClaI DraI DrdI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MslI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI ZraI