(Circular) (Six-base) MAPSORT of: pFN21AB6179.seq Check: 4385 from: 1 to: 6526 pFN21AB6179 6526 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2406 3869 1937 Size: 469 1463 4594 Fragments arranged by size: 4594 1463 469 Acc65I G'GTAC_C Cuts at: 3847 3847 Size: 6526 AclI AA'CG_TT Cuts at: 1563 4653 5026 1563 Size: 3090 373 3063 Fragments arranged by size: 3090 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3077 3150 3635 3713 4589 5973 6241 1632 Size: 1445 73 485 78 876 1384 268 1917 Fragments arranged by size: 1917 1445 1384 876 485 268 78 73 AflII C'TTAA_G Cuts at: 820 1017 2774 820 Size: 197 1757 4572 Fragments arranged by size: 4572 1757 197 AflIII A'CryG_T Cuts at: 1203 3288 3744 5425 6402 1203 Size: 2085 456 1681 977 1327 Fragments arranged by size: 2085 1681 1327 977 456 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 5255 5255 Size: 6526 AleI CACnn'nnGTG Cuts at: 1166 2414 1166 Size: 1248 5278 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6526 AlwNI CAG_nnn'CTG Cuts at: 2768 3664 3790 4269 5841 2768 Size: 896 126 479 1572 3453 Fragments arranged by size: 3453 1572 896 479 126 ApaI G_GGCC'C Cuts at: 2059 2059 Size: 6526 ApaLI G'TGCA_C Cuts at: 4583 5739 4583 Size: 1156 5370 ApoI r'AATT_y Cuts at: 1493 3835 4061 4213 6322 1493 Size: 2342 226 152 2109 1697 Fragments arranged by size: 2342 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2234 2697 3522 3851 1870 Size: 73 253 38 463 825 329 4545 Fragments arranged by size: 4545 825 463 329 253 73 38 BamHI G'GATC_C Cuts at: 3400 3856 4223 3400 Size: 456 367 5703 Fragments arranged by size: 5703 456 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2062 2086 3847 5302 Size: 325 217 627 224 51 24 1761 1455 Cuts at: 5302 618 Size: 1842 Fragments arranged by size: 1842 1761 1455 627 325 224 217 51 24 BanII G_rGCy'C Cuts at: 1391 2059 2235 3321 3629 3845 1391 Size: 668 176 1086 308 216 4072 Fragments arranged by size: 4072 1086 668 308 216 176 BbeI G_GCGC'C Cuts at: 2015 2066 2015 Size: 51 6475 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 2990 3623 928 Size: 971 617 474 633 3831 Fragments arranged by size: 3831 971 633 617 474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4717 4751 1903 Size: 34 2780 34 3678 Fragments arranged by size: 3678 2780 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2439 2632 4418 5628 2439 Size: 193 1786 1210 3337 Fragments arranged by size: 3337 1786 1210 193 BclI T'GATC_A Cuts at: 1548 2097 1548 Size: 549 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2246 5137 Size: 107 122 71 631 605 337 237 2891 Cuts at: 5137 136 Size: 1525 Fragments arranged by size: 2891 1525 631 605 337 237 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 3711 3943 3711 Size: 232 6294 Bme1580I G_kGCm'C Cuts at: 1790 2059 3181 4587 5743 1790 Size: 269 1122 1406 1156 2573 Fragments arranged by size: 2573 1406 1156 1122 269 BmgBI CAC'GTC Cuts at: 1318 2110 2193 1318 Size: 792 83 5651 Fragments arranged by size: 5651 792 83 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2351 2440 2728 Size: 416 53 83 399 24 934 89 288 Cuts at: 2728 4257 5215 442 Size: 1529 958 1753 Fragments arranged by size: 1753 1529 958 934 416 399 288 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3581 5186 1239 Size: 2342 1605 2579 Fragments arranged by size: 2579 2342 1605 Bpu10I CC'TnA_GC Cuts at: 2129 2145 2229 2129 Size: 16 84 6426 Fragments arranged by size: 6426 84 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3719 4651 5516 5814 3719 Size: 932 865 298 4431 Fragments arranged by size: 4431 932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3491 5189 882 Size: 617 1992 1698 2219 Fragments arranged by size: 2219 1992 1698 617 BsaAI yAC'GTr Cuts at: 493 1204 2306 3747 4317 493 Size: 711 1102 1441 570 2702 Fragments arranged by size: 2702 1441 1102 711 570 BsaBI GATnn'nnATC Cuts at: 2948 6520 2948 Size: 3572 2954 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2063 4333 4715 Size: 53 83 186 936 479 51 2270 382 Cuts at: 4715 5350 275 Size: 635 1451 Fragments arranged by size: 2270 1451 936 635 479 382 186 83 53 51 BsaWI w'CCGG_w Cuts at: 4959 5357 5631 5778 6189 4959 Size: 398 274 147 411 5296 Fragments arranged by size: 5296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6301 Fragments arranged by size: 6301 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2068 1186 Size: 882 5644 BseYI C'CCAG_C Cuts at: 2566 5729 6225 2566 Size: 3163 496 2867 Fragments arranged by size: 3163 2867 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 3176 1163 Size: 573 546 894 4513 Fragments arranged by size: 4513 894 573 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 3976 4737 5765 1540 Size: 275 186 156 1819 761 1028 2301 Fragments arranged by size: 2301 1819 1028 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2235 3306 4587 4672 5743 2235 Size: 1071 1281 85 1071 3018 Fragments arranged by size: 3018 1281 1071 1071 85 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2235 3181 3306 3321 3629 3845 Size: 399 269 176 946 125 15 308 216 Cuts at: 3845 4587 4672 5743 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 946 742 399 308 269 216 176 125 85 15 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2254 3436 3881 844 Size: 1410 1182 445 3489 Fragments arranged by size: 3489 1410 1182 445 BsrBI CCG'CTC Cuts at: 1394 1823 4413 1394 Size: 429 2590 3507 Fragments arranged by size: 3507 2590 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2905 5021 5195 65 Size: 1183 1657 2116 174 1396 Fragments arranged by size: 2116 1657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 5170 5357 1700 Size: 110 211 3149 187 2869 Fragments arranged by size: 3149 2869 211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 4586 5598 4586 Size: 1012 5514 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6526 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 3041 3793 1184 Size: 1857 752 3917 Fragments arranged by size: 3917 1857 752 BstYI r'GATC_y Cuts at: 1972 3400 3856 4223 4608 4625 6077 6163 6175 Size: 1428 456 367 385 17 1452 86 12 Cuts at: 6175 6521 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1428 456 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5035 Fragments arranged by size: 5035 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5787 Fragments arranged by size: 5787 683 56 BtsI GCAGTG_nn' Cuts at: 3596 4042 4836 4856 3596 Size: 446 794 20 5266 Fragments arranged by size: 5266 794 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 2647 3830 2647 Size: 1183 5343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3278 3500 5533 809 Size: 507 1962 222 2033 1802 Fragments arranged by size: 2033 1962 1802 507 222 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 3217 3973 Size: 54 1425 50 275 18 324 1063 756 Cuts at: 3973 4862 6491 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1063 889 756 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2154 3973 1537 Size: 275 342 1819 4090 Fragments arranged by size: 4090 1819 342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 3548 4456 1353 Size: 927 1268 908 3423 Fragments arranged by size: 3423 1268 927 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5113 5487 5633 387 Size: 1064 138 3524 374 146 1280 Fragments arranged by size: 3524 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2233 Size: 6526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3077 3150 3581 3635 3713 4589 5186 Size: 393 1445 73 431 54 78 876 597 Cuts at: 5186 5973 6241 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1445 876 787 597 431 393 268 78 73 54 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2144 Size: 6526 EcoO109I rG'GnC_Cy Cuts at: 2056 2590 2780 2056 Size: 534 190 5802 Fragments arranged by size: 5802 534 190 EcoRI G'AATT_C Cuts at: 3835 4213 3835 Size: 378 6148 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 2015 2066 2076 3718 3784 5673 1678 Size: 337 51 10 1642 66 1889 2531 Fragments arranged by size: 2531 1889 1642 337 66 51 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3088 3120 5167 5199 5241 Size: 32 135 32 1516 32 2047 32 42 Cuts at: 5241 5273 1373 Size: 32 2626 Fragments arranged by size: 2626 2047 1516 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3463 3870 4116 669 Size: 988 281 1525 407 246 3079 Fragments arranged by size: 3079 1525 988 407 281 246 HindIII A'AGCT_T Cuts at: 748 2531 748 Size: 1783 4743 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KasI G'GCGC_C Cuts at: 2011 2062 2011 Size: 51 6475 KpnI G_GTAC'C Cuts at: 3851 3851 Size: 6526 MfeI C'AATT_G Cuts at: 4125 4125 Size: 6526 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2456 2528 5640 5824 1305 Size: 1151 72 3112 184 2007 Fragments arranged by size: 3112 2007 1151 184 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4704 Fragments arranged by size: 4704 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2525 2690 4484 4843 5002 518 Size: 648 1248 111 165 1794 359 159 2042 Fragments arranged by size: 2042 1794 1248 648 359 165 159 111 MspA1I CmG'CkG Cuts at: 1606 2095 3942 4619 5767 6012 1606 Size: 489 1847 677 1148 245 2120 Fragments arranged by size: 2120 1847 1148 677 489 245 NaeI GCC'GGC Cuts at: 2023 2023 Size: 6526 NarI GG'CG_CC Cuts at: 2012 2063 2012 Size: 51 6475 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5035 Fragments arranged by size: 5035 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NgoMIV G'CCGG_C Cuts at: 2021 2021 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 3884 5429 1132 Size: 97 2655 1545 2229 Fragments arranged by size: 2655 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6526 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2819 3790 3814 1108 Size: 1711 971 24 3820 Fragments arranged by size: 3820 1711 971 24 PfoI T'CCnGG_A Cuts at: 3813 3813 Size: 6526 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2590 2590 Size: 6526 PshAI GACnn'nnGTC Cuts at: 1613 2699 3458 1613 Size: 1086 759 4681 Fragments arranged by size: 4681 1086 759 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 2055 2055 Size: 6526 PspXI vC'TCGA_Gb Cuts at: 2234 2234 Size: 6526 PstI C_TGCA'G Cuts at: 830 3315 3606 3878 830 Size: 2485 291 272 3478 Fragments arranged by size: 3478 2485 291 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 SacI G_AGCT'C Cuts at: 2235 2235 Size: 6526 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SapI GCTCTTCn'nnn_ Cuts at: 3548 3548 Size: 6526 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2629 2937 3311 3602 3874 5009 5690 Size: 219 1584 308 374 291 272 1135 681 Cuts at: 5690 5881 826 Size: 191 1471 Fragments arranged by size: 1584 1471 1135 681 374 308 291 272 219 191 SfoI GGC'GCC Cuts at: 2013 2064 2013 Size: 51 6475 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 3524 3853 1872 Size: 1652 329 4545 Fragments arranged by size: 4545 1652 329 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 2774 3734 4630 5531 5793 Size: 197 926 291 540 960 896 901 262 Cuts at: 5793 820 Size: 1553 Fragments arranged by size: 1553 960 926 901 896 540 291 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 2728 4450 5 Size: 47 2676 1722 2081 Fragments arranged by size: 2676 2081 1722 47 StuI AGG'CCT Cuts at: 1517 3477 3578 1517 Size: 1960 101 4465 Fragments arranged by size: 4465 1960 101 StyI C'CwwG_G Cuts at: 513 1065 2004 3396 3965 513 Size: 552 939 1392 569 3074 Fragments arranged by size: 3074 1392 939 569 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2426 2459 3029 4553 4570 4723 4908 Size: 1255 846 33 570 1524 17 153 185 Cuts at: 4908 5353 325 Size: 445 1498 Fragments arranged by size: 1524 1498 1255 846 570 445 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2650 4772 6484 Size: 274 80 33 51 1443 673 2122 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 2122 1712 1443 673 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3385 3417 6486 6518 3385 Size: 32 3069 32 3393 Fragments arranged by size: 3393 3069 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6526 XbaI T'CTAG_A Cuts at: 3862 3862 Size: 6526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2996 2996 Size: 6526 XhoI C'TCGA_G Cuts at: 1943 2234 1943 Size: 291 6235 XmaI C'CCGG_G Cuts at: 1870 3522 3851 1870 Size: 1652 329 4545 Fragments arranged by size: 4545 1652 329 XmnI GAAnn'nnTTC Cuts at: 4655 4655 Size: 6526 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI