(Circular) (Six-base) MAPSORT of: pFN21AB6174.seq Check: 815 from: 1 to: 6595 pFN21AB6174 6595 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2591 3025 3398 3472 2591 Size: 434 373 74 5714 Fragments arranged by size: 5714 434 373 74 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4405 278 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3042 3669 3938 1937 Size: 1105 627 269 4594 Fragments arranged by size: 4594 1105 627 269 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 2459 4722 5095 1563 Size: 896 2263 373 3063 Fragments arranged by size: 3063 2263 896 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4658 6042 6310 1632 Size: 3026 1384 268 1917 Fragments arranged by size: 3026 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 5494 6471 1203 Size: 4291 977 1327 Fragments arranged by size: 4291 1327 977 AgeI A'CCGG_T Cuts at: 5426 5426 Size: 6595 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 3791 1166 Size: 2625 3970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3494 1184 Size: 2310 4285 AlwNI CAG_nnn'CTG Cuts at: 2944 3246 3257 4338 5910 2944 Size: 302 11 1081 1572 3629 Fragments arranged by size: 3629 1572 1081 302 11 ApaI G_GGCC'C Cuts at: 2042 2168 3494 2042 Size: 126 1326 5143 Fragments arranged by size: 5143 1326 126 ApaLI G'TGCA_C Cuts at: 4652 5808 4652 Size: 1156 5439 ApoI r'AATT_y Cuts at: 1493 3161 3904 4130 4282 6391 1493 Size: 1668 743 226 152 2109 1697 Fragments arranged by size: 2109 1697 1668 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 5149 160 Size: 872 4117 1606 Fragments arranged by size: 4117 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 2338 3920 1870 Size: 73 395 1582 4545 Fragments arranged by size: 4545 1582 395 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3828 3861 3828 Size: 33 6562 BamHI G'GATC_C Cuts at: 2130 3925 4292 2130 Size: 1795 367 4433 Fragments arranged by size: 4433 1795 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2435 2760 2796 2925 3201 Size: 325 217 627 648 325 36 129 276 Cuts at: 3201 3321 3484 3643 3916 5371 618 Size: 120 163 159 273 1455 1842 Fragments arranged by size: 1842 1455 648 627 325 325 276 273 217 163 159 129 120 36 BanII G_rGCy'C Cuts at: 1391 2042 2117 2168 2938 2979 3001 3494 3914 Size: 651 75 51 770 41 22 493 420 Cuts at: 3914 1391 Size: 4072 Fragments arranged by size: 4072 770 651 493 420 75 51 41 22 BbeI G_GCGC'C Cuts at: 2764 2929 3205 2764 Size: 165 276 6154 Fragments arranged by size: 6154 276 165 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2568 928 Size: 971 669 4955 Fragments arranged by size: 4955 971 669 BbvCI CC'TCA_GC Cuts at: 2010 2010 Size: 6595 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4786 4820 1903 Size: 34 2849 34 3678 Fragments arranged by size: 3678 2849 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3583 4487 5697 3583 Size: 904 1210 4481 Fragments arranged by size: 4481 1210 904 BclI T'GATC_A Cuts at: 1548 3795 1548 Size: 2247 4348 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2228 2275 5206 Size: 107 122 71 631 605 556 47 2931 Cuts at: 5206 136 Size: 1525 Fragments arranged by size: 2931 1525 631 605 556 122 107 71 47 BglII A'GATC_T Cuts at: 6590 6590 Size: 6595 BlpI GC'TnA_GC Cuts at: 4012 4012 Size: 6595 Bme1580I G_kGCm'C Cuts at: 1790 2042 2168 3494 4656 5812 1790 Size: 252 126 1326 1162 1156 2573 Fragments arranged by size: 2573 1326 1162 1156 252 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6595 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3234 3526 4326 Size: 416 53 83 399 24 1817 292 800 Cuts at: 4326 5284 442 Size: 958 1753 Fragments arranged by size: 1817 1753 958 800 416 399 292 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3900 5255 1239 Size: 2661 1355 2579 Fragments arranged by size: 2661 2579 1355 Bpu10I CC'TnA_GC Cuts at: 2010 2010 Size: 6595 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4720 5585 5883 4720 Size: 865 298 5432 Fragments arranged by size: 5432 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2342 2759 5258 882 Size: 617 843 417 2499 2219 Fragments arranged by size: 2499 2219 843 617 417 BsaAI yAC'GTr Cuts at: 493 1204 4386 493 Size: 711 3182 2702 Fragments arranged by size: 3182 2702 711 BsaBI GATnn'nnATC Cuts at: 6589 6589 Size: 6595 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2761 2926 3202 4402 Size: 53 83 186 936 1228 165 276 1200 Cuts at: 4402 4784 5419 275 Size: 382 635 1451 Fragments arranged by size: 1451 1228 1200 936 635 382 276 186 165 83 53 BsaWI w'CCGG_w Cuts at: 2652 5028 5426 5700 5847 6258 2652 Size: 2376 398 274 147 411 2989 Fragments arranged by size: 2989 2376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2634 2664 3373 3403 3518 Size: 30 165 30 1231 30 709 30 115 Cuts at: 3518 3548 1178 Size: 30 4225 Fragments arranged by size: 4225 1231 709 165 115 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3728 3740 1186 Size: 2542 12 4041 Fragments arranged by size: 4041 2542 12 BseYI C'CCAG_C Cuts at: 2374 2937 3305 5798 6294 2374 Size: 563 368 2493 496 2675 Fragments arranged by size: 2675 2493 563 496 368 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 3123 3500 1163 Size: 573 826 561 377 4258 Fragments arranged by size: 4258 826 573 561 377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4045 4806 5834 1540 Size: 275 186 2044 761 1028 2301 Fragments arranged by size: 2301 2044 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2482 3539 4656 4741 5812 2482 Size: 1057 1117 85 1071 3265 Fragments arranged by size: 3265 1117 1071 1057 85 BsmI GAATG_Cn' Cuts at: 4106 4199 4106 Size: 93 6502 BsmBI CGTCTCn'nnnn_ Cuts at: 2635 2635 Size: 6595 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2117 2168 2482 2938 2979 3001 Size: 399 252 75 51 314 456 41 22 Cuts at: 3001 3494 3539 3914 4656 4741 5812 1391 Size: 493 45 375 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 493 456 399 375 314 252 85 75 51 45 41 22 BspEI T'CCGG_A Cuts at: 2652 6258 2652 Size: 3606 2989 BspHI T'CATG_A Cuts at: 4484 6214 4484 Size: 1730 4865 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2591 3025 3398 3472 3883 3950 844 Size: 1747 434 373 74 411 67 3489 Fragments arranged by size: 3489 1747 434 411 373 74 67 BsrBI CCG'CTC Cuts at: 1394 1823 4482 1394 Size: 429 2659 3507 Fragments arranged by size: 3507 2659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2719 5090 5264 65 Size: 1183 1471 2371 174 1396 Fragments arranged by size: 2371 1471 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3075 5239 5426 1700 Size: 110 1265 2164 187 2869 Fragments arranged by size: 2869 2164 1265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6595 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 4655 5667 4655 Size: 1012 5583 BstAPI GCAn_nnn'nTGC Cuts at: 794 2211 794 Size: 1417 5178 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6595 BstXI CCAn_nnnn'nTGG Cuts at: 3001 3539 3001 Size: 538 6057 BstYI r'GATC_y Cuts at: 1972 2130 3925 4292 4677 4694 6146 6232 6244 Size: 158 1795 367 385 17 1452 86 12 Cuts at: 6244 6590 1972 Size: 346 1977 Fragments arranged by size: 1977 1795 1452 385 367 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2238 513 Size: 552 939 234 4870 Fragments arranged by size: 4870 939 552 234 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 2942 4111 4905 4925 2942 Size: 1169 794 20 4612 Fragments arranged by size: 4612 1169 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6560 DraI TTT'AAA Cuts at: 3899 3899 Size: 6595 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5602 809 Size: 507 4286 1802 Fragments arranged by size: 4286 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 3130 4042 Size: 54 1425 50 275 18 886 414 912 Cuts at: 4042 4931 6560 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 912 889 886 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4042 1537 Size: 275 2230 4090 Fragments arranged by size: 4090 2230 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2192 3762 4525 1353 Size: 839 1570 763 3423 Fragments arranged by size: 3423 1570 839 763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3133 3602 5182 5556 5702 387 Size: 1064 138 1544 469 1580 374 146 1280 Fragments arranged by size: 1580 1544 1280 1064 469 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3900 4658 5255 6042 6310 1239 Size: 393 2268 758 597 787 268 1524 Fragments arranged by size: 2268 1524 787 758 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3521 3521 Size: 6595 EcoO109I rG'GnC_Cy Cuts at: 2039 2165 2335 2571 3096 3491 2039 Size: 126 170 236 525 395 5143 Fragments arranged by size: 5143 525 395 236 170 126 EcoRI G'AATT_C Cuts at: 3904 4282 3904 Size: 378 6217 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3367 3367 Size: 6595 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2056 2764 2929 3187 3205 5742 1678 Size: 378 708 165 258 18 2537 2531 Fragments arranged by size: 2537 2531 708 378 258 165 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 2597 2629 2634 Size: 32 135 32 543 32 450 32 5 Cuts at: 2634 2666 3731 3763 5236 5268 5310 5342 1373 Size: 32 1065 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 1065 543 450 135 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 3939 4185 669 Size: 988 281 2001 246 3079 Fragments arranged by size: 3079 2001 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6595 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KasI G'GCGC_C Cuts at: 2760 2925 3201 2760 Size: 165 276 6154 Fragments arranged by size: 6154 276 165 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 4194 4194 Size: 6595 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2284 3416 5709 5893 1305 Size: 979 1132 2293 184 2007 Fragments arranged by size: 2293 2007 1132 979 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2718 10 Size: 54 1425 343 886 3887 Fragments arranged by size: 3887 1425 886 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3791 4553 4912 5071 518 Size: 648 2625 762 359 159 2042 Fragments arranged by size: 2625 2042 762 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2449 2596 3135 3620 4011 4688 5836 6081 Size: 843 147 539 485 391 677 1148 245 Cuts at: 6081 1606 Size: 2120 Fragments arranged by size: 2120 1148 843 677 539 485 391 245 147 NaeI GCC'GGC Cuts at: 3077 3077 Size: 6595 NarI GG'CG_CC Cuts at: 2761 2926 3202 2761 Size: 165 276 6154 Fragments arranged by size: 6154 276 165 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5104 Fragments arranged by size: 5104 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6595 NgoMIV G'CCGG_C Cuts at: 3075 3075 Size: 6595 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 2882 3953 5498 1132 Size: 97 1653 1071 1545 2229 Fragments arranged by size: 2229 1653 1545 1071 97 PasI CC'CwG_GG Cuts at: 1348 2035 1348 Size: 687 5908 PciI A'CATG_T Cuts at: 5494 5494 Size: 6595 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2608 3246 1108 Size: 1131 369 638 4457 Fragments arranged by size: 4457 1131 638 369 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4664 4696 6177 6209 4664 Size: 32 1481 32 5050 Fragments arranged by size: 5050 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6595 PsiI TTA'TAA Cuts at: 4165 4165 Size: 6595 PspOMI G'GGCC_C Cuts at: 2038 2164 3490 2038 Size: 126 1326 5143 Fragments arranged by size: 5143 1326 126 PstI C_TGCA'G Cuts at: 830 2595 3216 3947 830 Size: 1765 621 731 3478 Fragments arranged by size: 3478 1765 731 621 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 PvuII CAG'CTG Cuts at: 2596 3620 2596 Size: 1024 5571 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SbfI CC_TGCA'GG Cuts at: 3947 3947 Size: 6595 ScaI AGT'ACT Cuts at: 4843 6555 4843 Size: 1712 4883 SfcI C'TryA_G Cuts at: 826 1045 2591 3056 3212 3943 5078 5759 5950 Size: 219 1546 465 156 731 1135 681 191 Cuts at: 5950 826 Size: 1471 Fragments arranged by size: 1546 1471 1135 731 681 465 219 191 156 SfoI GGC'GCC Cuts at: 2762 2927 3203 2762 Size: 165 276 6154 Fragments arranged by size: 6154 276 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 3922 1872 Size: 2050 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4699 5600 5862 820 Size: 197 926 2756 901 262 1553 Fragments arranged by size: 2756 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 3953 1132 Size: 2821 3774 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 3678 1517 Size: 2161 4434 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2352 3351 3854 4034 513 Size: 552 939 222 126 999 503 180 3074 Fragments arranged by size: 3074 999 939 552 503 222 180 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2385 2453 3806 4622 4639 4792 4977 Size: 1255 805 68 1353 816 17 153 185 Cuts at: 4977 5422 325 Size: 445 1498 Fragments arranged by size: 1498 1353 1255 816 805 445 185 153 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4841 6553 96 Size: 274 80 33 51 1443 2864 1712 138 Fragments arranged by size: 2864 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3112 3144 3518 3550 6555 6587 3112 Size: 32 374 32 3005 32 3120 Fragments arranged by size: 3120 3005 374 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6595 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2711 2711 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 3920 1870 Size: 2050 4545 XmnI GAAnn'nnTTC Cuts at: 2064 4724 2064 Size: 2660 3935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4403 276 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI