Restriction map for pFN21AB6174
 (Linear) (Six-base) MAPSORT of: KIBB6174.seq  Check: 4773  from: 1  to: 1890



KIBB6174.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     586    1020    1393    1467    1890 
   Size:        586     434     373      74     423

  Fragments arranged by size: 

                 586     434     423     373      74
 
AccI GT'mk_AC

Cuts at:      0    1037    1664    1890 
   Size:       1037     627     226

  Fragments arranged by size: 

                1037     627     226
 
AclI AA'CG_TT

Cuts at:      0     454    1890 
   Size:        454    1436
 
AleI CACnn'nnGTG

Cuts at:      0    1786    1890 
   Size:       1786     104
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1489    1890 
   Size:       1489     401
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1241    1252    1890 
   Size:        939     302      11     638

  Fragments arranged by size: 

                 939     638     302      11
 
ApaI G_GGCC'C

Cuts at:      0      37     163    1489    1890 
   Size:         37     126    1326     401

  Fragments arranged by size: 

                1326     401     126      37
 
ApoI r'AATT_y

Cuts at:      0    1156    1890 
   Size:       1156     734
 
AvaI C'yCGr_G

Cuts at:      0     333    1890 
   Size:        333    1557
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1823    1856    1890 
   Size:       1823      33      34

  Fragments arranged by size: 

                1823      34      33
 
BamHI G'GATC_C

Cuts at:      0     125    1890 
   Size:        125    1765
 
BanI G'GyrC_C

Cuts at:      0     430     755     791     920    1196    1316    1479    1638 
   Size:        430     325      36     129     276     120     163     159

Cuts at:   1638    1890 
   Size:        252

  Fragments arranged by size: 

                 430     325     276     252     163     159     129     120
                  36
 
BanII G_rGCy'C

Cuts at:      0      37     112     163     933     974     996    1489    1890 
   Size:         37      75      51     770      41      22     493     401

  Fragments arranged by size: 

                 770     493     401      75      51      41      37      22
            
 
BbeI G_GCGC'C

Cuts at:      0     759     924    1200    1890 
   Size:        759     165     276     690

  Fragments arranged by size: 

                 759     690     276     165
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     563    1890 
   Size:        563    1327
 
BbvCI CC'TCA_GC

Cuts at:      0       5    1890 
   Size:          5    1885
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1578    1890 
   Size:       1578     312
 
BclI T'GATC_A

Cuts at:      0    1790    1890 
   Size:       1790     100
 
BglI GCCn_nnn'nGGC

Cuts at:      0     223     270    1890 
   Size:        223      47    1620

  Fragments arranged by size: 

                1620     223      47
 
Bme1580I G_kGCm'C

Cuts at:      0      37     163    1489    1890 
   Size:         37     126    1326     401

  Fragments arranged by size: 

                1326     401     126      37
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1229    1521    1890 
   Size:       1229     292     369

  Fragments arranged by size: 

                1229     369     292
 
Bpu10I CC'TnA_GC

Cuts at:      0       5    1890 
   Size:          5    1885
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     337     754    1890 
   Size:        337     417    1136

  Fragments arranged by size: 

                1136     417     337
 
BsaHI Gr'CG_yC

Cuts at:      0     756     921    1197    1890 
   Size:        756     165     276     693

  Fragments arranged by size: 

                 756     693     276     165
 
BsaWI w'CCGG_w

Cuts at:      0     647    1890 
   Size:        647    1243
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     629     659    1368    1398    1513    1543    1890 
   Size:        629      30     709      30     115      30     347

  Fragments arranged by size: 

                 709     629     347     115      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1723    1735    1890 
   Size:       1723      12     155

  Fragments arranged by size: 

                1723     155      12
 
BseYI C'CCAG_C

Cuts at:      0     369     932    1300    1890 
   Size:        369     563     368     590

  Fragments arranged by size: 

                 590     563     369     368
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1118    1495    1890 
   Size:        557     561     377     395

  Fragments arranged by size: 

                 561     557     395     377
 
BsiHKAI G_wGCw'C

Cuts at:      0     477    1534    1890 
   Size:        477    1057     356

  Fragments arranged by size: 

                1057     477     356
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     630    1890 
   Size:        630    1260
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     112     163     477     933     974     996    1489 
   Size:         37      75      51     314     456      41      22     493

Cuts at:   1489    1534    1890 
   Size:         45     356

  Fragments arranged by size: 

                 493     456     356     314      75      51      45      41
                  37      22
 
BspEI T'CCGG_A

Cuts at:      0     647    1890 
   Size:        647    1243
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     586    1020    1393    1467    1890 
   Size:        586     434     373      74     423

  Fragments arranged by size: 

                 586     434     423     373      74
 
BsrDI GCAATG_nn'

Cuts at:      0     714    1890 
   Size:        714    1176
 
BsrFI r'CCGG_y

Cuts at:      0    1070    1890 
   Size:       1070     820
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     206    1890 
   Size:        206    1684
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     996    1534    1890 
   Size:        996     538     356

  Fragments arranged by size: 

                 996     538     356
 
BstYI r'GATC_y

Cuts at:      0     125    1890 
   Size:        125    1765
 
BtgI C'CryG_G

Cuts at:      0     233    1890 
   Size:        233    1657
 
BtsI GCAGTG_nn'

Cuts at:      0     937    1890 
   Size:        937     953
 
EaeI y'GGCC_r

Cuts at:      0     711    1125    1890 
   Size:        711     414     765

  Fragments arranged by size: 

                 765     711     414
 
EarI CTCTTCn'nnn_

Cuts at:      0     187    1757    1890 
   Size:        187    1570     133

  Fragments arranged by size: 

                1570     187     133
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1128    1597    1890 
   Size:       1128     469     293

  Fragments arranged by size: 

                1128     469     293
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1516    1890 
   Size:       1516     374
 
EcoO109I rG'GnC_Cy

Cuts at:      0      34     160     330     566    1091    1486    1890 
   Size:         34     126     170     236     525     395     404

  Fragments arranged by size: 

                 525     404     395     236     170     126      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1362    1890 
   Size:       1362     528
 
HaeII r_GCGC'y

Cuts at:      0      51     759     924    1182    1200    1890 
   Size:         51     708     165     258      18     690

  Fragments arranged by size: 

                 708     690     258     165      51      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142     592     624     629     661    1726    1758 
   Size:        110      32     450      32       5      32    1065      32

Cuts at:   1758    1890 
   Size:        132

  Fragments arranged by size: 

                1065     450     132     110      32      32      32      32
                   5
 
KasI G'GCGC_C

Cuts at:      0     755     920    1196    1890 
   Size:        755     165     276     694

  Fragments arranged by size: 

                 755     694     276     165
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     279    1411    1890 
   Size:        279    1132     479

  Fragments arranged by size: 

                1132     479     279
 
MscI TGG'CCA

Cuts at:      0     713    1890 
   Size:        713    1177
 
MslI CAynn'nnrTG

Cuts at:      0    1786    1890 
   Size:       1786     104
 
MspA1I CmG'CkG

Cuts at:      0     444     591    1130    1615    1890 
   Size:        444     147     539     485     275

  Fragments arranged by size: 

                 539     485     444     275     147
 
NaeI GCC'GGC

Cuts at:      0    1072    1890 
   Size:       1072     818
 
NarI GG'CG_CC

Cuts at:      0     756     921    1197    1890 
   Size:        756     165     276     693

  Fragments arranged by size: 

                 756     693     276     165
 
NgoMIV G'CCGG_C

Cuts at:      0    1070    1890 
   Size:       1070     820
 
NspI r_CATG'y

Cuts at:      0     877    1890 
   Size:        877    1013
 
PasI CC'CwG_GG

Cuts at:      0      30    1890 
   Size:         30    1860
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234     603    1241    1890 
   Size:        234     369     638     649

  Fragments arranged by size: 

                 649     638     369     234
 
PspOMI G'GGCC_C

Cuts at:      0      33     159    1485    1890 
   Size:         33     126    1326     405

  Fragments arranged by size: 

                1326     405     126      33
 
PstI C_TGCA'G

Cuts at:      0     590    1211    1890 
   Size:        590     621     679

  Fragments arranged by size: 

                 679     621     590
 
PvuII CAG'CTG

Cuts at:      0     591    1615    1890 
   Size:        591    1024     275

  Fragments arranged by size: 

                1024     591     275
 
SfcI C'TryA_G

Cuts at:      0     586    1051    1207    1890 
   Size:        586     465     156     683

  Fragments arranged by size: 

                 683     586     465     156
 
SfoI GGC'GCC

Cuts at:      0     757     922    1198    1890 
   Size:        757     165     276     692

  Fragments arranged by size: 

                 757     692     276     165
 
StuI AGG'CCT

Cuts at:      0    1673    1890 
   Size:       1673     217
 
StyI C'CwwG_G

Cuts at:      0     221     347    1346    1849    1890 
   Size:        221     126     999     503      41

  Fragments arranged by size: 

                 999     503     221     126      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     380     448    1801    1890 
   Size:        380      68    1353      89

  Fragments arranged by size: 

                1353     380      89      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1107    1139    1513    1545    1890 
   Size:       1107      32     374      32     345

  Fragments arranged by size: 

                1107     374     345      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     706    1890 
   Size:        706    1184
 
XmnI GAAnn'nnTTC

Cuts at:      0      59    1890 
   Size:         59    1831
 

 Enzymes that do cut:

     AarI     AccI     AclI     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI Bme1580I     BmrI   Bpu10I     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI
     BtsI     EaeI     EarI     EciI    EcoNI EcoO109I     FalI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI    PflMI   PspOMI     PstI    PvuII     SfcI
     SfoI     StuI     StyI    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BcgI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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