Restriction map for
pFN21AB5669
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiHKAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
FbaI FseI FspI HaeII Hin1I
HpaI HphI KasI KpnI MamI
MfeI MluI MroI MslI MunI
NarI NcoI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 589 GTAGAC GT^[AC][GT]AC
AflIII 503 ACACGT A^C[GA][CT]GT
AlwNI 337 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 610 CCTCAGG CC^T[ACGT]AGG
AseI 226 ATTAAT AT^TAAT
AsnI 226 ATTAAT AT^TAAT
AvaI 103 CCCGAG C^[CT]CG[GA]G
BalI 132 TGGCCA TGG^CCA
BbsI 499 GAAGAC GAAGAC(2/6)
BbvI 51 GCTGC GCAGC(8/12)
BcnI 189 CCCGG CC^[GC]GG
BglI 28 GCCGGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 256 GCTAAGC GC^T[ACGT]AGC
BmyI 1011 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 256 GCTAAGC GC^T[ACGT]AGC
BpuAI 499 GAAGAC GAAGAC(2/6)
BsiEI 67 CGGCCG CG[GA][CT]^CG
BsmI 923 GAATGC GAATGC(1/-1)
BsoBI 103 CCCGAG C^[CT]CG[GA]G
Bsp1286I 1011 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 441 TGTACA T^GTACA
BsrFI 28 GCCGGC [GA]^CCGG[CT]
BsrGI 441 TGTACA T^GTACA
BstBI 920 TTCGAA TT^CGAA
BstNI 135 CCAGG CC^[AT]GG
Bsu36I 610 CCTCAGG CC^T[ACGT]AGG
CelII 256 GCTAAGC GC^T[ACGT]AGC
CfoI 49 GCGC GCG^C
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
EagI 67 CGGCCG C^GGCCG
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
Eco81I 610 CCTCAGG CC^T[ACGT]AGG
EcoRI 268 GAATTC G^AATTC
EcoRII 135 CCAGG ^CC[AT]GG
EcoT22I 964 ATGCAT ATGCA^T
HgaI 93 GCGTC GACGC(5/10)
HhaI 49 GCGC GCG^C
HinP1I 49 GCGC G^CGC
HincII 728 GTCAAC GT[CT]^[GA]AC
HindII 728 GTCAAC GT[CT]^[GA]AC
KspI 64 CCGCGG CCGC^GG
MaeII 505 ACGT A^CGT
MluNI 132 TGGCCA TGG^CCA
MscI 132 TGGCCA TGG^CCA
MvaI 135 CCAGG CC^[AT]GG
NaeI 28 GCCGGC GCC^GGC
NciI 189 CCCGG CC^[GC]GG
NdeI 788 CATATG CA^TATG
NgoMIV 28 GCCGGC G^CCGGC
NsiI 964 ATGCAT ATGCA^T
NspI 966 GCATGC [GA]CATG^[CT]
NspV 920 TTCGAA TT^CGAA
PshBI 226 ATTAAT AT^TAAT
PvuII 541 CAGCTG CAG^CTG
SacII 64 CCGCGG CCGC^GG
SfuI 920 TTCGAA TT^CGAA
SphI 966 GCATGC GCATG^C
SspBI 441 TGTACA T^GTACA
SspI 775 AATATT AAT^ATT
TaqI 921 TCGA T^CGA
TfiI 950 GAATC G^A[AT]TC
TseI 51 GCTGC G^C[AT]GC
TthHB8I 921 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 442 GTAC GT^AC
616 GTAC
BamHI 6 GGATCC G^GATCC
148 GGATCC
BfaI 284 CTAG C^TAG
812 CTAG
BpmI 165 CTCCAG CTGGAG(16/14)
55 CTGGAG
BsaWI 432 ACCGGA [AT]^CCGG[AT]
462 TCCGGT
BseRI 82 GAGGAG GAGGAG(10/8)
287 GAGGAG
BsmAI 139 GAGAC GTCTC(1/5)
312 GAGAC
BsmFI 95 GTCCC GGGAC(10/14)
101 GTCCC
BssKI 135 CCAGG ^CC[ACGT]GG
189 CCCGG
BstYI 6 GGATCC [GA]^GATC[CT]
148 GGATCC
DsaI 38 CCGTGG C^C[GA][CT]GG
64 CCGCGG
EaeI 67 CGGCCG [CT]^GGCC[GA]
132 TGGCCA
EarI 993 CTCTTC CTCTTC(1/4)
309 GAAGAG
FauI 46 CCCGC CCCGC(4/6)
77 GCGGG
HindIII 259 AAGCTT A^AGCTT
274 AAGCTT
Ksp632I 993 CTCTTC CTCTTC(1/4)
309 GAAGAG
MaeI 284 CTAG C^TAG
812 CTAG
MaeIII 176 GTGAC ^GT[ACGT]AC
185 GTGAC
MflI 6 GGATCC [GA]^GATC[CT]
148 GGATCC
MspA1I 64 CCGCGG C[AC]G^C[GT]G
541 CAGCTG
NlaIII 445 CATG CATG^
967 CATG
PleI 892 GACTC GAGTC(4/5)
58 GAGTC
RsaI 442 GTAC GT^AC
616 GTAC
ScrFI 135 CCAGG CC^[ACGT]GG
189 CCCGG
SfaNI 345 GATGC GCATC(5/9)
557 GCATC
Tsp45I 176 GTGAC ^GT[GC]AC
185 GTGAC
XhoII 6 GGATCC [GA]^GATC[CT]
148 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI BsaJI BsiYI BslI BsrI
BstF5I BstUI Cac8I DdeI DpnII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MboI MboII MnlI
MseI MspI MvnI MwoI NdeII
NlaIV Sau3AI Tru9I Tsp509I TspRI