(Circular) (Six-base) MAPSORT of: pFN21AB5669.seq Check: 4118 from: 1 to: 5752 pFN21AB5669 5752 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3562 278 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2595 3095 1937 Size: 658 500 4594 Fragments arranged by size: 4594 658 500 Acc65I G'GTAC_C Cuts at: 3073 3073 Size: 5752 AclI AA'CG_TT Cuts at: 1563 3879 4252 1563 Size: 2316 373 3063 Fragments arranged by size: 3063 2316 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2170 2918 3815 5199 5467 1632 Size: 538 748 897 1384 268 1917 Fragments arranged by size: 1917 1384 897 748 538 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5555 AflIII A'CryG_T Cuts at: 1203 2508 4651 5628 1203 Size: 1305 2143 977 1327 Fragments arranged by size: 2143 1327 1305 977 AgeI A'CCGG_T Cuts at: 4583 4583 Size: 5752 AhdI GACnn_n'nnGTC Cuts at: 4481 4481 Size: 5752 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5752 AlwNI CAG_nnn'CTG Cuts at: 2347 3495 5067 2347 Size: 1148 1572 3032 Fragments arranged by size: 3032 1572 1148 ApaLI G'TGCA_C Cuts at: 3809 4965 3809 Size: 1156 4596 ApoI r'AATT_y Cuts at: 1493 2129 2273 2462 2800 3061 3287 3439 5548 Size: 636 144 189 338 261 226 152 2109 Cuts at: 5548 1493 Size: 1697 Fragments arranged by size: 2109 1697 636 338 261 226 189 152 144 AseI AT'TA_AT Cuts at: 160 1032 2232 4306 160 Size: 872 1200 2074 1606 Fragments arranged by size: 2074 1606 1200 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3077 1870 Size: 73 165 969 4545 Fragments arranged by size: 4545 969 165 73 BamHI G'GATC_C Cuts at: 2011 2153 3082 3449 2011 Size: 142 929 367 4314 Fragments arranged by size: 4314 929 367 142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 4528 618 Size: 325 217 627 1286 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 217 BanII G_rGCy'C Cuts at: 1391 3071 1391 Size: 1680 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2511 928 Size: 971 612 4169 Fragments arranged by size: 4169 971 612 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3943 3977 1903 Size: 34 2006 34 3678 Fragments arranged by size: 3678 2006 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3644 4854 3644 Size: 1210 4542 BclI T'GATC_A Cuts at: 1548 1548 Size: 5752 BfrBI ATG'CAT Cuts at: 2971 2971 Size: 5752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 4363 136 Size: 107 122 71 631 605 367 2324 1525 Fragments arranged by size: 2324 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 5747 5747 Size: 5752 BlpI GC'TnA_GC Cuts at: 2262 3169 2262 Size: 907 4845 Bme1580I G_kGCm'C Cuts at: 1790 3020 3813 4969 1790 Size: 1230 793 1156 2573 Fragments arranged by size: 2573 1230 1156 793 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2097 3483 4441 Size: 416 53 83 399 24 680 1386 958 Cuts at: 4441 442 Size: 1753 Fragments arranged by size: 1753 1386 958 680 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2155 4412 1239 Size: 842 74 2257 2579 Fragments arranged by size: 2579 2257 842 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3877 4742 5040 3877 Size: 865 298 4589 Fragments arranged by size: 4589 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4415 882 Size: 617 2916 2219 Fragments arranged by size: 2916 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3543 493 Size: 711 2339 2702 Fragments arranged by size: 2702 2339 711 BsaBI GATnn'nnATC Cuts at: 5746 5746 Size: 5752 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3559 3941 4576 275 Size: 53 83 186 936 2026 382 635 1451 Fragments arranged by size: 2026 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2437 2467 4185 4583 4857 5004 5415 2437 Size: 30 1718 398 274 147 411 2774 Fragments arranged by size: 2774 1718 411 398 274 147 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5527 Fragments arranged by size: 5527 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2307 1186 Size: 916 205 4631 Fragments arranged by size: 4631 916 205 BseYI C'CCAG_C Cuts at: 4955 5451 4955 Size: 496 5256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2075 3202 3963 4991 1540 Size: 275 186 74 1127 761 1028 2301 Fragments arranged by size: 2301 1127 1028 761 275 186 74 BsiHKAI G_wGCw'C Cuts at: 3813 3898 4969 3813 Size: 85 1071 4596 Fragments arranged by size: 4596 1071 85 BsmI GAATG_Cn' Cuts at: 2934 3263 3356 2934 Size: 329 93 5330 Fragments arranged by size: 5330 329 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3020 3071 3813 3898 4969 1391 Size: 399 1230 51 742 85 1071 2174 Fragments arranged by size: 2174 1230 1071 742 399 85 51 BspEI T'CCGG_A Cuts at: 5415 5415 Size: 5752 BspHI T'CATG_A Cuts at: 3641 5371 3641 Size: 1730 4022 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 844 Size: 2263 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3639 1394 Size: 429 1816 3507 Fragments arranged by size: 3507 1816 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4247 4421 65 Size: 1183 2999 174 1396 Fragments arranged by size: 2999 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2033 4396 4583 1700 Size: 110 223 2363 187 2869 Fragments arranged by size: 2869 2363 223 187 110 BsrGI T'GTAC_A Cuts at: 96 2446 96 Size: 2350 3402 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5752 BssSI C'ACGA_G Cuts at: 3812 4824 3812 Size: 1012 4740 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5752 BstBI TT'CG_AA Cuts at: 2926 4556 2926 Size: 1630 4122 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5752 BstYI r'GATC_y Cuts at: 1972 2011 2153 3082 3449 3834 3851 5303 5389 Size: 39 142 929 367 385 17 1452 86 Cuts at: 5389 5401 5747 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 929 385 367 346 142 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2616 2616 Size: 5752 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2069 513 Size: 552 939 39 26 4196 Fragments arranged by size: 4196 939 552 39 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5013 Fragments arranged by size: 5013 683 56 BtsI GCAGTG_nn' Cuts at: 3268 4062 4082 3268 Size: 794 20 4938 Fragments arranged by size: 4938 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5752 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5717 DraI TTT'AAA Cuts at: 3056 3056 Size: 5752 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4759 809 Size: 507 3443 1802 Fragments arranged by size: 3443 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2137 3199 Size: 54 1425 50 275 18 242 65 1062 Cuts at: 3199 4088 5717 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1062 889 275 242 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2072 3199 1537 Size: 275 260 1127 4090 Fragments arranged by size: 4090 1127 275 260 EarI CTCTTCn'nnn_ Cuts at: 1353 2309 3004 3682 1353 Size: 956 695 678 3423 Fragments arranged by size: 3423 956 695 678 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4339 4713 4859 387 Size: 1064 138 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2155 2170 2918 3815 4412 5199 Size: 393 449 74 15 748 897 597 787 Cuts at: 5199 5467 1239 Size: 268 1524 Fragments arranged by size: 1524 897 787 748 597 449 393 268 74 15 EcoRI G'AATT_C Cuts at: 2273 3061 3439 2273 Size: 788 378 4586 Fragments arranged by size: 4586 788 378 FspI TGC'GCA Cuts at: 4258 4258 Size: 5752 HaeII r_GCGC'y Cuts at: 1678 4899 1678 Size: 3221 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373 Size: 32 135 32 2821 32 42 32 2626 Fragments arranged by size: 2821 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2735 3096 3342 669 Size: 988 281 797 361 246 3079 Fragments arranged by size: 3079 988 797 361 281 246 HindIII A'AGCT_T Cuts at: 748 2264 2279 748 Size: 1516 15 4221 Fragments arranged by size: 4221 1516 15 HpaI GTT'AAC Cuts at: 3342 3342 Size: 5752 KpnI G_GTAC'C Cuts at: 3077 3077 Size: 5752 MfeI C'AATT_G Cuts at: 3351 3351 Size: 5752 MluI A'CGCG_T Cuts at: 5628 5628 Size: 5752 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4866 5050 1305 Size: 3561 184 2007 Fragments arranged by size: 3561 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 10 Size: 54 1425 343 307 3623 Fragments arranged by size: 3623 1425 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 3710 4069 4228 518 Size: 648 2544 359 159 2042 Fragments arranged by size: 2544 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2548 3168 3845 4993 5238 1606 Size: 465 477 620 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 620 477 465 245 NaeI GCC'GGC Cuts at: 2035 2035 Size: 5752 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4261 Fragments arranged by size: 4261 939 552 NdeI CA'TA_TG Cuts at: 387 2794 387 Size: 2407 3345 NgoMIV G'CCGG_C Cuts at: 2033 2033 Size: 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5752 NotI GC'GGCC_GC Cuts at: 3199 3199 Size: 5752 NsiI A_TGCA'T Cuts at: 2973 2973 Size: 5752 NspI r_CATG'y Cuts at: 1132 1229 2975 3110 4655 1132 Size: 97 1746 135 1545 2229 Fragments arranged by size: 2229 1746 1545 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5752 PciI A'CATG_T Cuts at: 4651 4651 Size: 5752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5752 PmeI GTTT'AAAC Cuts at: 3056 3056 Size: 5752 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3821 3853 5334 5366 3821 Size: 32 1481 32 4207 Fragments arranged by size: 4207 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5752 PsiI TTA'TAA Cuts at: 2334 3322 2334 Size: 988 4764 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2289 2321 2289 Size: 32 5720 PstI C_TGCA'G Cuts at: 830 3104 830 Size: 2274 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5752 PvuII CAG'CTG Cuts at: 2548 2548 Size: 5752 SacII CC_GC'GG Cuts at: 2072 2072 Size: 5752 SalI G'TCGA_C Cuts at: 1936 3094 1936 Size: 1158 4594 SbfI CC_TGCA'GG Cuts at: 3104 3104 Size: 5752 ScaI AGT'ACT Cuts at: 4000 5712 4000 Size: 1712 4040 SfcI C'TryA_G Cuts at: 826 1045 3100 4235 4916 5107 826 Size: 219 2055 1135 681 191 1471 Fragments arranged by size: 2055 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5752 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5752 SmaI CCC'GGG Cuts at: 1872 3079 1872 Size: 1207 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3856 4757 5019 820 Size: 197 926 1913 901 262 1553 Fragments arranged by size: 1913 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3543 493 Size: 3050 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5752 SphI G_CATG'C Cuts at: 1132 2975 3110 1132 Size: 1843 135 3774 Fragments arranged by size: 3774 1843 135 SspI AAT'ATT Cuts at: 5 52 2782 3676 5 Size: 47 2730 894 2081 Fragments arranged by size: 2730 2081 894 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5752 StyI C'CwwG_G Cuts at: 513 1065 2004 3191 513 Size: 552 939 1187 3074 Fragments arranged by size: 3074 1187 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2931 3779 3796 3949 4134 4579 325 Size: 1255 1351 848 17 153 185 445 1498 Fragments arranged by size: 1498 1351 1255 848 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2446 3998 5710 Size: 274 80 33 51 1443 469 1552 1712 Cuts at: 5710 96 Size: 138 Fragments arranged by size: 1712 1552 1443 469 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5712 5744 5712 Size: 32 5720 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5752 XbaI T'CTAG_A Cuts at: 3088 3088 Size: 5752 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5752 XmaI C'CCGG_G Cuts at: 1870 3077 1870 Size: 1207 4545 XmnI GAAnn'nnTTC Cuts at: 3881 3881 Size: 5752 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3560 276 Size: 53 83 186 936 2026 2468 Fragments arranged by size: 2468 2026 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI