Restriction map for
pFN21AB5632
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI FbaI FokI FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 183 GTCTAC GT^[AC][GT]AC
AcsI 474 GAATTT [GA]^AATT[CT]
AlwI 697 GGATC GGATC(4/5)
AlwNI 773 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 708 GGGCCC GGGCC^C
ApoI 474 GAATTT [GA]^AATT[CT]
AvaII 525 GGTCC G^G[AT]CC
AvrII 528 CCTAGG C^CTAGG
BanI 391 GGCACC G^G[CT][GA]CC
BcgI 639 GCAGGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 20 CCGGG CC^[GC]GG
BlnI 528 CCTAGG C^CTAGG
BseRI 644 CTCCTC GAGGAG(10/8)
BsmFI 654 GTCCC GGGAC(10/14)
BspMI 169 ACCTGC ACCTGC(4/8)
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BstYI 697 GGATCT [GA]^GATC[CT]
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
DpnII 698 GATC ^GATC
DraI 307 TTTAAA TTT^AAA
DraII 707 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 424 GACATCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 707 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 793 GATATC GAT^ATC
EcoT22I 350 ATGCAT ATGCA^T
FauI 200 GCGGG CCCGC(4/6)
HgaI 7 GCGTC GACGC(5/10)
HincII 204 GTTGAC GT[CT]^[GA]AC
HindII 204 GTTGAC GT[CT]^[GA]AC
MboI 698 GATC ^GATC
MboII 137 TCTTC GAAGA(8/7)
MflI 697 GGATCT [GA]^GATC[CT]
NaeI 47 GCCGGC GCC^GGC
NciI 20 CCGGG CC^[GC]GG
NdeII 698 GATC ^GATC
NgoMIV 47 GCCGGC G^CCGGC
NlaIII 552 CATG CATG^
NsiI 350 ATGCAT ATGCA^T
PshAI 207 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 606 CTGCAG CTGCA^G
Sau3AI 698 GATC ^GATC
SpeI 187 ACTAGT A^CTAGT
XhoII 697 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanII 85 GGGCTC G[GA]GC[CT]^C
708 GGGCCC
BfaI 188 CTAG C^TAG
529 CTAG
BmyI 85 GGGCTC G[AGT]GC[ACT]^C
708 GGGCCC
BsaI 332 GAGACC GGTCTC(1/5)
532 GGTCTC
BsiYI 14 CCGGAGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
228 CCAGCCAGAGG
BslI 14 CCGGAGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
228 CCAGCCAGAGG
Bsp1286I 85 GGGCTC G[AGT]GC[ACT]^C
708 GGGCCC
CfoI 51 GCGC GCG^C
79 GCGC
DdeI 210 CTCAG C^T[ACGT]AG
668 CTGAG
HaeII 50 GGCGCT [GA]GCGC^[CT]
78 GGCGCT
HaeIII 240 GGCC GG^CC
709 GGCC
HhaI 51 GCGC GCG^C
79 GCGC
HinP1I 51 GCGC G^CGC
79 GCGC
MaeI 188 CTAG C^TAG
529 CTAG
MspA1I 95 CAGCTG C[AC]G^C[GT]G
321 CAGCTG
MwoI 4 GCAGCGTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
25 GCGGTGATTGC
PleI 684 GAGTC GAGTC(4/5)
775 GAGTC
PvuII 95 CAGCTG CAG^CTG
321 CAGCTG
SfaNI 349 GATGC GCATC(5/9)
352 GCATC
SfcI 570 CTATAG C^T[GA][CT]AG
606 CTGCAG
TfiI 317 GAATC G^A[AT]TC
555 GATTC
Tsp45I 627 GTCAC ^GT[GC]AC
755 GTGAC
Tsp509I 295 AATT ^AATT
475 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI BbvI BpmI
BsaJI BsmAI BsrI BssKI BstNI
Cac8I Cfr13I EcoRII EcoT14I Fnu4HI
HapII HinfI HpaII HphI ItaI
MaeIII MnlI MseI MslI MspI
MvaI NlaIV RsaI Sau96I ScrFI
StyI Tru9I TseI TspRI