Restriction map for
pFN21AB5632
(Linear) (Six-base) MAPSORT of: KIBB5632.seq Check: 3585 from: 1 to: 807
KIBB5632.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 184 807
Size: 184 623
AleI CACnn'nnGTG
Cuts at: 0 250 807
Size: 250 557
AlwNI CAG_nnn'CTG
Cuts at: 0 778 807
Size: 778 29
ApaI G_GGCC'C
Cuts at: 0 712 807
Size: 712 95
ApoI r'AATT_y
Cuts at: 0 474 807
Size: 474 333
AvrII C'CTAG_G
Cuts at: 0 528 807
Size: 528 279
BanI G'GyrC_C
Cuts at: 0 391 807
Size: 391 416
BanII G_rGCy'C
Cuts at: 0 89 712 807
Size: 89 623 95
Fragments arranged by size:
623 95 89
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 662 807
Size: 628 34 145
Fragments arranged by size:
628 145 34
BfrBI ATG'CAT
Cuts at: 0 352 807
Size: 352 455
Bme1580I G_kGCm'C
Cuts at: 0 712 807
Size: 712 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 706 807
Size: 706 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 240 350 807
Size: 119 121 110 457
Fragments arranged by size:
457 121 119 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 326 538 807
Size: 326 212 269
Fragments arranged by size:
326 269 212
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 635 807
Size: 635 172
BseYI C'CCAG_C
Cuts at: 0 323 807
Size: 323 484
Bsp1286I G_dGCh'C
Cuts at: 0 89 712 807
Size: 89 623 95
Fragments arranged by size:
623 95 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 178 807
Size: 178 629
BsrFI r'CCGG_y
Cuts at: 0 47 807
Size: 47 760
BstYI r'GATC_y
Cuts at: 0 697 807
Size: 697 110
DraI TTT'AAA
Cuts at: 0 309 807
Size: 309 498
DrdI GACnn_nn'nnGTC
Cuts at: 0 430 807
Size: 430 377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 758 807
Size: 758 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 240 350 807
Size: 119 121 110 457
Fragments arranged by size:
457 121 119 110
EcoO109I rG'GnC_Cy
Cuts at: 0 708 807
Size: 708 99
EcoRV GAT'ATC
Cuts at: 0 795 807
Size: 795 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 304 807
Size: 304 503
HaeII r_GCGC'y
Cuts at: 0 54 82 807
Size: 54 28 725
Fragments arranged by size:
725 54 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 807
Size: 610 32 165
Fragments arranged by size:
610 165 32
HincII GTy'rAC
Cuts at: 0 206 807
Size: 206 601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 807
Size: 686 121
MslI CAynn'nnrTG
Cuts at: 0 250 550 752 807
Size: 250 300 202 55
Fragments arranged by size:
300 250 202 55
MspA1I CmG'CkG
Cuts at: 0 97 323 807
Size: 97 226 484
Fragments arranged by size:
484 226 97
NaeI GCC'GGC
Cuts at: 0 49 807
Size: 49 758
NgoMIV G'CCGG_C
Cuts at: 0 47 807
Size: 47 760
NsiI A_TGCA'T
Cuts at: 0 354 807
Size: 354 453
PfoI T'CCnGG_A
Cuts at: 0 217 807
Size: 217 590
PshAI GACnn'nnGTC
Cuts at: 0 211 807
Size: 211 596
PspOMI G'GGCC_C
Cuts at: 0 708 807
Size: 708 99
PstI C_TGCA'G
Cuts at: 0 610 807
Size: 610 197
PvuII CAG'CTG
Cuts at: 0 97 323 807
Size: 97 226 484
Fragments arranged by size:
484 226 97
SfcI C'TryA_G
Cuts at: 0 570 606 807
Size: 570 36 201
Fragments arranged by size:
570 201 36
SpeI A'CTAG_T
Cuts at: 0 187 807
Size: 187 620
StyI C'CwwG_G
Cuts at: 0 110 273 288 528 807
Size: 110 163 15 240 279
Fragments arranged by size:
279 240 163 110 15
TatI w'GTAC_w
Cuts at: 0 610 807
Size: 610 197
Enzymes that do cut:
AccI AleI AlwNI ApaI ApoI AvrII BanI BanII
BcgI BfrBI Bme1580I BmrI BpmI BsaI BseRI BseYI
Bsp1286I BspMI BsrFI BstYI DraI DrdI EciI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII MmeI MslI
MspA1I NaeI NgoMIV NsiI PfoI PshAI PspOMI PstI
PvuII SfcI SpeI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI
EarI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI