(Circular) (Six-base) MAPSORT of: pFN21AB5558.seq Check: 5689 from: 1 to: 6424 pFN21AB5558 6424 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3443 3443 Size: 6424 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4234 278 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2136 3767 1937 Size: 199 1631 4594 Fragments arranged by size: 4594 1631 199 Acc65I G'GTAC_C Cuts at: 3745 3745 Size: 6424 AclI AA'CG_TT Cuts at: 1563 4551 4924 1563 Size: 2988 373 3063 Fragments arranged by size: 3063 2988 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2520 2850 2873 3236 4487 5871 6139 1632 Size: 888 330 23 363 1251 1384 268 1917 Fragments arranged by size: 1917 1384 1251 888 363 330 268 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6227 AflIII A'CryG_T Cuts at: 1203 5323 6300 1203 Size: 4120 977 1327 Fragments arranged by size: 4120 1327 977 AgeI A'CCGG_T Cuts at: 2041 5255 2041 Size: 3214 3210 AhdI GACnn_n'nnGTC Cuts at: 5153 5153 Size: 6424 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6424 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6424 AlwNI CAG_nnn'CTG Cuts at: 4167 5739 4167 Size: 1572 4852 ApaLI G'TGCA_C Cuts at: 4481 5637 4481 Size: 1156 5268 ApoI r'AATT_y Cuts at: 1493 2096 2717 2754 3423 3648 3733 3959 4111 Size: 603 621 37 669 225 85 226 152 Cuts at: 4111 6220 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 669 621 603 226 225 152 85 37 AseI AT'TA_AT Cuts at: 160 1032 2691 4978 160 Size: 872 1659 2287 1606 Fragments arranged by size: 2287 1659 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2112 3749 1870 Size: 73 130 39 1637 4545 Fragments arranged by size: 4545 1637 130 73 39 BamHI G'GATC_C Cuts at: 3754 4121 3754 Size: 367 6057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3745 5200 618 Size: 325 217 627 1958 1455 1842 Fragments arranged by size: 1958 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3743 1391 Size: 2352 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2879 3015 928 Size: 971 980 136 4337 Fragments arranged by size: 4337 980 971 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4615 4649 1903 Size: 34 50 34 2594 34 3678 Fragments arranged by size: 3678 2594 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2774 4316 5526 2774 Size: 1542 1210 3672 Fragments arranged by size: 3672 1542 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6424 BfrBI ATG'CAT Cuts at: 3345 3345 Size: 6424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5035 136 Size: 107 122 71 631 605 3363 1525 Fragments arranged by size: 3363 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6419 6419 Size: 6424 BlpI GC'TnA_GC Cuts at: 3841 3841 Size: 6424 Bme1580I G_kGCm'C Cuts at: 1790 3334 4485 5641 1790 Size: 1544 1151 1156 2573 Fragments arranged by size: 2573 1544 1156 1151 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6424 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4155 5113 442 Size: 416 53 83 399 24 2738 958 1753 Fragments arranged by size: 2738 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2895 3354 3546 5084 1239 Size: 1656 459 192 1538 2579 Fragments arranged by size: 2579 1656 1538 459 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4549 5414 5712 4549 Size: 865 298 5261 Fragments arranged by size: 5261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2032 5087 882 Size: 617 533 3055 2219 Fragments arranged by size: 3055 2219 617 533 BsaAI yAC'GTr Cuts at: 493 1204 4215 493 Size: 711 3011 2702 Fragments arranged by size: 3011 2702 711 BsaBI GATnn'nnATC Cuts at: 6418 6418 Size: 6424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4231 4613 5248 275 Size: 53 83 186 936 2698 382 635 1451 Fragments arranged by size: 2698 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2041 4857 5255 5529 5676 6087 2041 Size: 2816 398 274 147 411 2378 Fragments arranged by size: 2816 2378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3195 3225 3519 3549 1178 Size: 30 165 30 1792 30 294 30 4053 Fragments arranged by size: 4053 1792 294 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2052 1186 Size: 866 5558 BseYI C'CCAG_C Cuts at: 2330 5627 6123 2330 Size: 3297 496 2631 Fragments arranged by size: 3297 2631 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2991 1163 Size: 573 388 867 4596 Fragments arranged by size: 4596 867 573 388 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3874 4635 5663 1540 Size: 275 186 1873 761 1028 2301 Fragments arranged by size: 2301 1873 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4485 4570 5641 4485 Size: 85 1071 5268 Fragments arranged by size: 5268 1071 85 BsmI GAATG_Cn' Cuts at: 3345 3454 3935 4028 3345 Size: 109 481 93 5741 Fragments arranged by size: 5741 481 109 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3334 3743 4485 4570 5641 1391 Size: 399 1544 409 742 85 1071 2174 Fragments arranged by size: 2174 1544 1071 742 409 399 85 BspEI T'CCGG_A Cuts at: 6087 6087 Size: 6424 BspHI T'CATG_A Cuts at: 3022 4313 6043 3022 Size: 1291 1730 3403 Fragments arranged by size: 3403 1730 1291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2788 3443 3779 844 Size: 1944 655 336 3489 Fragments arranged by size: 3489 1944 655 336 BsrBI CCG'CTC Cuts at: 1394 1823 4311 1394 Size: 429 2488 3507 Fragments arranged by size: 3507 2488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2577 3438 3690 4919 5093 65 Size: 1183 1329 861 252 1229 174 1396 Fragments arranged by size: 1396 1329 1229 1183 861 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 5068 5255 1700 Size: 110 231 3027 187 2869 Fragments arranged by size: 3027 2869 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6424 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6424 BssSI C'ACGA_G Cuts at: 4484 5496 4484 Size: 1012 5412 BstAPI GCAn_nnn'nTGC Cuts at: 794 2516 794 Size: 1722 4702 BstBI TT'CG_AA Cuts at: 5228 5228 Size: 6424 BstEII G'GTnAC_C Cuts at: 1184 2013 1184 Size: 829 5595 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2056 Size: 6424 BstYI r'GATC_y Cuts at: 1972 3464 3754 4121 4506 4523 5975 6061 6073 Size: 1492 290 367 385 17 1452 86 12 Cuts at: 6073 6419 1972 Size: 346 1977 Fragments arranged by size: 1977 1492 1452 385 367 346 290 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5872 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5685 Fragments arranged by size: 5685 683 56 BtsI GCAGTG_nn' Cuts at: 2346 2979 3940 4734 4754 2346 Size: 633 961 794 20 4016 Fragments arranged by size: 4016 961 794 633 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6389 DraI TTT'AAA Cuts at: 3728 3728 Size: 6424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5431 809 Size: 507 4115 1802 Fragments arranged by size: 4115 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 3871 4760 Size: 54 1425 50 275 18 327 1714 889 Cuts at: 4760 6389 8 Size: 1629 43 Fragments arranged by size: 1714 1629 1425 889 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3871 1537 Size: 275 2059 4090 Fragments arranged by size: 4090 2059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 3654 4354 1353 Size: 1626 675 700 3423 Fragments arranged by size: 3423 1626 700 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5011 5385 5531 387 Size: 1064 138 3422 374 146 1280 Fragments arranged by size: 3422 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2520 2850 2873 2895 3236 3354 3546 Size: 393 888 330 23 22 341 118 192 Cuts at: 3546 4487 5084 5871 6139 1239 Size: 941 597 787 268 1524 Fragments arranged by size: 1524 941 888 787 597 393 341 330 268 192 118 23 22 EcoO109I rG'GnC_Cy Cuts at: 3667 3667 Size: 6424 EcoRI G'AATT_C Cuts at: 2717 3733 4111 2717 Size: 1016 378 5030 Fragments arranged by size: 5030 1016 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6424 FspI TGC'GCA Cuts at: 4930 4930 Size: 6424 HaeII r_GCGC'y Cuts at: 1678 5571 1678 Size: 3893 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2986 3018 5065 5097 5139 Size: 32 135 32 1414 32 2047 32 42 Cuts at: 5139 5171 1373 Size: 32 2626 Fragments arranged by size: 2626 2047 1414 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3768 4014 669 Size: 988 281 1683 147 246 3079 Fragments arranged by size: 3079 1683 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 6424 HpaI GTT'AAC Cuts at: 3621 4014 3621 Size: 393 6031 KpnI G_GTAC'C Cuts at: 3749 3749 Size: 6424 MfeI C'AATT_G Cuts at: 2253 4023 2253 Size: 1770 4654 MluI A'CGCG_T Cuts at: 6300 6300 Size: 6424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 3019 5538 5722 1305 Size: 1258 456 2519 184 2007 Fragments arranged by size: 2519 2007 1258 456 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4602 Fragments arranged by size: 4602 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4382 4741 4900 518 Size: 648 3216 359 159 2042 Fragments arranged by size: 3216 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3840 4517 5665 5910 1606 Size: 2234 677 1148 245 2120 Fragments arranged by size: 2234 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5872 NdeI CA'TA_TG Cuts at: 387 387 Size: 6424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6424 NotI GC'GGCC_GC Cuts at: 3871 3871 Size: 6424 NsiI A_TGCA'T Cuts at: 3347 3347 Size: 6424 NspI r_CATG'y Cuts at: 1132 1229 3721 3782 5327 1132 Size: 97 2492 61 1545 2229 Fragments arranged by size: 2492 2229 1545 97 61 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6424 PciI A'CATG_T Cuts at: 5323 5323 Size: 6424 PflMI CCAn_nnn'nTGG Cuts at: 1108 2918 1108 Size: 1810 4614 PfoI T'CCnGG_A Cuts at: 2721 2721 Size: 6424 PmeI GTTT'AAAC Cuts at: 3728 3728 Size: 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 6006 6038 4493 Size: 32 1481 32 4879 Fragments arranged by size: 4879 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3667 3667 Size: 6424 PshAI GACnn'nnGTC Cuts at: 1613 2044 1613 Size: 431 5993 PsiI TTA'TAA Cuts at: 3994 3994 Size: 6424 PstI C_TGCA'G Cuts at: 830 3776 830 Size: 2946 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6424 SalI G'TCGA_C Cuts at: 1936 3766 1936 Size: 1830 4594 SbfI CC_TGCA'GG Cuts at: 3776 3776 Size: 6424 ScaI AGT'ACT Cuts at: 4672 6384 4672 Size: 1712 4712 SfcI C'TryA_G Cuts at: 826 1045 2370 3624 3772 4907 5588 5779 826 Size: 219 1325 1254 148 1135 681 191 1471 Fragments arranged by size: 1471 1325 1254 1135 681 219 191 148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6424 SmaI CCC'GGG Cuts at: 1872 3751 1872 Size: 1879 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4528 5429 5691 820 Size: 197 926 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4215 493 Size: 3722 2702 SpeI A'CTAG_T Cuts at: 152 3149 152 Size: 2997 3427 SphI G_CATG'C Cuts at: 1132 3782 1132 Size: 2650 3774 SspI AAT'ATT Cuts at: 5 52 4348 5 Size: 47 4296 2081 Fragments arranged by size: 4296 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6424 StyI C'CwwG_G Cuts at: 513 1065 2376 3354 3863 513 Size: 552 1311 978 509 3074 Fragments arranged by size: 3074 1311 978 552 509 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3677 4451 4468 4621 4806 5251 Size: 1255 2088 9 774 17 153 185 445 Cuts at: 5251 325 Size: 1498 Fragments arranged by size: 2088 1498 1255 774 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2356 2855 4670 Size: 274 80 33 51 1443 379 499 1815 Cuts at: 4670 6382 96 Size: 1712 138 Fragments arranged by size: 1815 1712 1443 499 379 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2172 2204 3195 3227 3519 3551 3634 3666 6384 Size: 32 991 32 292 32 83 32 2718 Cuts at: 6384 6416 2172 Size: 32 2180 Fragments arranged by size: 2718 2180 991 292 83 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6424 XbaI T'CTAG_A Cuts at: 3760 3760 Size: 6424 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6424 XmaI C'CCGG_G Cuts at: 1870 3749 1870 Size: 1879 4545 XmnI GAAnn'nnTTC Cuts at: 3012 4553 3012 Size: 1541 4883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4232 276 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI