Restriction map for pFN21AB5535
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BbrPI       BbvCI       BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       BstAPI      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       EcoT22I     FauI        
FseI        FspI        HgaI        HpaI        KpnI        
KspI        MaeII       MamI        MluI        NaeI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          815  GTATAC               GT^[AC][GT]AC
AccII         148  CGCG                 CG^CG
AccIII       1316  TCCGGA               T^CCGGA
AcsI          471  GAATTC               [GA]^AATT[CT]
AcyI           58  GGCGCC               G[GA]^CG[CT]C
AflII         466  CTTAAG               C^TTAAG
AocI          853  CCTCAGG              CC^T[ACGT]AGG
ApoI          471  GAATTC               [GA]^AATT[CT]
BamHI        1319  GGATCC               G^GATCC
BbeI           58  GGCGCC               GGCGC^C
BcgI           72  CGACACACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         213  GGATAC               GTATCC(6/5)
BclI          510  TGATCA               T^GATCA
BfaI          799  CTAG                 C^TAG
BfrI          466  CTTAAG               C^TTAAG
BsaHI          58  GGCGCC               G[GA]^CG[CT]C
BsaI         1181  GAGACC               GGTCTC(1/5)
BseAI        1316  TCCGGA               T^CCGGA
BseRI        1023  CTCCTC               GAGGAG(10/8)
BspEI        1316  TCCGGA               T^CCGGA
BsrDI        1064  GCAATG               GCAATG(2/0)
BsrFI        1291  ACCGGC               [GA]^CCGG[CT]
Bst1107I      815  GTATAC               GTA^TAC
BstBI         746  TTCGAA               TT^CGAA
BstEII        339  GGTGACC              G^GT[ACGT]ACC
BstPI         339  GGTGACC              G^GT[ACGT]ACC
BstUI         148  CGCG                 CG^CG
BstZ17I       815  GTATAC               GTA^TAC
Bsu36I        853  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1291  ACCGGC               [GA]^CCGG[CT]
DraII          10  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1106  CCATGG               C^C[GA][CT]GG
EarI          325  GAAGAG               CTCTTC(1/4)
Eco81I        853  CCTCAGG              CC^T[ACGT]AGG
EcoO109I       10  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       339  GGTGACC              G^GT[ACGT]ACC
EcoRI         471  GAATTC               G^AATTC
FbaI          510  TGATCA               T^GATCA
HaeII          58  GGCGCC               [GA]GCGC^[CT]
Hin1I          58  GGCGCC               G[GA]^CG[CT]C
KasI           58  GGCGCC               G^GCGCC
Ksp632I       325  GAAGAG               CTCTTC(1/4)
MaeI          799  CTAG                 C^TAG
MaeIII        340  GTGAC                ^GT[ACGT]AC
MfeI         1346  CAATTG               C^AATTG
MroI         1316  TCCGGA               T^CCGGA
MslI         1356  CATAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         1346  CAATTG               C^AATTG
MvnI          148  CGCG                 CG^CG
NarI           58  GGCGCC               GG^CGCC
NcoI         1106  CCATGG               C^CATGG
NdeI          221  CATATG               CA^TATG
NspV          746  TTCGAA               TT^CGAA
SfaNI         757  GCATC                GCATC(5/9)
SfoI           58  GGCGCC               GGC^GCC
SfuI          746  TTCGAA               TT^CGAA
Tsp45I        340  GTGAC                ^GT[GC]AC
XcmI          753  CCAAGCATCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI           10  GGGCCC               GGGCC^C
             1102  GGGCCC              
AvaII         172  GGACC                G^G[AT]CC
             1092  GGTCC               
BalI          243  TGGCCA               TGG^CCA
              618  TGGCCA              
BbsI         1003  GAAGAC               GAAGAC(2/6)
              118  GTCTTC              
BpmI          132  CTCCAG               CTGGAG(16/14)
              708  CTGGAG              
BpuAI        1003  GAAGAC               GAAGAC(2/6)
              118  GTCTTC              
BsaWI         412  ACCGGA               [AT]^CCGG[AT]
             1316  TCCGGA              
BsgI           87  CTGCAC               GTGCAG(16/14)
              650  CTGCAC              
BsmFI         171  GGGAC                GGGAC(10/14)
              704  GGGAC               
BstF5I        758  CATCC                GGATG(2/0)
             1256  GGATG               
BstYI        1157  GGATCT               [GA]^GATC[CT]
             1319  GGATCC              
Cac8I          24  GCTCGC               GC[ACGT]^[ACGT]GC
              958  GCTTGC              
EaeI          243  TGGCCA               [CT]^GGCC[GA]
              618  TGGCCA              
FokI          758  CATCC                GGATG(9/13)
             1256  GGATG               
HincII        154  GTCAAC               GT[CT]^[GA]AC
              451  GTCAAC              
HindII        154  GTCAAC               GT[CT]^[GA]AC
              451  GTCAAC              
HindIII       956  AAGCTT               A^AGCTT
             1081  AAGCTT              
MflI         1157  GGATCT               [GA]^GATC[CT]
             1319  GGATCC              
MluNI         243  TGGCCA               TGG^CCA
              618  TGGCCA              
MscI          243  TGGCCA               TGG^CCA
              618  TGGCCA              
PleI          582  GACTC                GAGTC(4/5)
             1243  GACTC               
PvuII         768  CAGCTG               CAG^CTG
             1281  CAGCTG              
TaqI          627  TCGA                 T^CGA
              747  TCGA                
TfiI          809  GAATC                G^A[AT]TC
              982  GATTC               
TthHB8I       627  TCGA                 T^CGA
              747  TCGA                
XhoII        1157  GGATCT               [GA]^GATC[CT]
             1319  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanI        BanII       BbvI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       Bsp1286I    BspMI       
BsrI        BssKI       BstNI       BstXI       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       SmlI        
StyI        Tru9I       TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com