Restriction map for
pFN21AB5503
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BsaBI BsaWI BseAI
BsgI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BspMI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 727 GTCTAC GT^[AC][GT]AC
AcyI 1437 GGCGTC G[GA]^CG[CT]C
AhdI 709 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1339 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 400 CCTCAGG CC^T[ACGT]AGG
Aor51HI 640 AGCGCT AGC^GCT
ApaI 487 GGGCCC GGGCC^C
AspEI 709 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 961 GTGCTC G[AT]GC[AT]^C
AvaI 41 CCCGGG C^[CT]CG[GA]G
AvaII 336 GGACC G^G[AT]CC
BamHI 934 GGATCC G^GATCC
BglI 7 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1616 AGATCT A^GATCT
BlpI 1198 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1198 GCTCAGC GC^T[ACGT]AGC
BsaHI 1437 GGCGTC G[GA]^CG[CT]C
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiHKAI 961 GTGCTC G[AT]GC[AT]^C
BsoBI 41 CCCGGG C^[CT]CG[GA]G
BsrBI 97 CCGCTC CCGCTC(-3/-3)
BsrDI 832 GCAATG GCAATG(2/0)
BsrFI 1271 ACCGGC [GA]^CCGG[CT]
BssHII 51 GCGCGC G^CGCGC
Bsu36I 400 CCTCAGG CC^T[ACGT]AGG
CelII 1198 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1271 ACCGGC [GA]^CCGG[CT]
DrdI 1629 GACCCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 113 CCACGG C^C[GA][CT]GG
EagI 5 CGGCCG C^GGCCG
Eam1105I 709 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5 CGGCCG C^GGCCG
Eco47III 640 AGCGCT AGC^GCT
Eco52I 5 CGGCCG C^GGCCG
Eco81I 400 CCTCAGG CC^T[ACGT]AGG
EcoRV 438 GATATC GAT^ATC
HgaI 1438 GCGTC GACGC(5/10)
Hin1I 1437 GGCGTC G[GA]^CG[CT]C
HincII 1090 GTTGAC GT[CT]^[GA]AC
HindII 1090 GTTGAC GT[CT]^[GA]AC
NotI 4 GCGGCCGC GC^GGCCGC
NspI 887 ACATGC [GA]CATG^[CT]
PvuII 1207 CAGCTG CAG^CTG
SfcI 1403 CTGTAG C^T[GA][CT]AG
SmaI 41 CCCGGG CCC^GGG
SmlI 661 CTCAAG C^T[CT][GA]AG
TaqI 1133 TCGA T^CGA
TthHB8I 1133 TCGA T^CGA
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 52 CGCG CG^CG
209 CGCG
AlwI 935 GATCC GGATC(4/5)
934 GGATC
BbsI 108 GAAGAC GAAGAC(2/6)
1656 GAAGAC
BfaI 31 CTAG C^TAG
1267 CTAG
BpuAI 108 GAAGAC GAAGAC(2/6)
1656 GAAGAC
BsaAI 433 TACGTG [CT]AC^GT[GA]
979 TACGTG
BsaI 118 GAGACC GGTCTC(1/5)
1627 GAGACC
BsmAI 118 GAGAC GTCTC(1/5)
1627 GAGAC
BsmI 162 GAATGC GAATGC(1/-1)
777 GAATGC
Bsp1407I 1007 TGTACA T^GTACA
1394 TGTACA
BsrGI 1007 TGTACA T^GTACA
1394 TGTACA
BstUI 52 CGCG CG^CG
209 CGCG
BstXI 80 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
147 CCAGATCAGTGG
BstYI 934 GGATCC [GA]^GATC[CT]
1616 AGATCT
DraII 350 AGGCCCT [GA]G^G[ACGT]CC[CT]
1233 AGGGCCT
EcoNI 320 CCTATCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
621 CCTTCCAGAGG
EcoO109I 350 AGGCCCT [GA]G^G[ACGT]CC[CT]
1233 AGGGCCT
MaeI 31 CTAG C^TAG
1267 CTAG
MflI 934 GGATCC [GA]^GATC[CT]
1616 AGATCT
MseI 536 TTAA T^TAA
1370 TTAA
MslI 195 CACTGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CACCGTTGTG
MvnI 52 CGCG CG^CG
209 CGCG
SfaNI 1384 GCATC GCATC(5/9)
1453 GCATC
SspBI 1007 TGTACA T^GTACA
1394 TGTACA
StuI 317 AGGCCT AGG^CCT
530 AGGCCT
Tru9I 536 TTAA T^TAA
1370 TTAA
XcmI 625 CCAGAGGACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
872 CCAGCTACTATTTGG
XhoII 934 GGATCC [GA]^GATC[CT]
1616 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I TspRI