(Circular) (Six-base) MAPSORT of: pFN21AB5503.seq Check: 56 from: 1 to: 6370 pFN21AB5503 6370 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2733 3713 1937 Size: 796 980 4594 Fragments arranged by size: 4594 980 796 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2680 4433 5817 6085 1632 Size: 527 521 1753 1384 268 1917 Fragments arranged by size: 1917 1753 1384 527 521 268 AfeI AGC'GCT Cuts at: 2647 2647 Size: 6370 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 5269 6246 1203 Size: 4066 977 1327 Fragments arranged by size: 4066 1327 977 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 2719 5099 2719 Size: 2380 3990 AleI CACnn'nnGTG Cuts at: 1166 2204 2261 1166 Size: 1038 57 5275 Fragments arranged by size: 5275 1038 57 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6370 AlwNI CAG_nnn'CTG Cuts at: 3349 4113 5685 3349 Size: 764 1572 4034 Fragments arranged by size: 4034 1572 764 ApaI G_GGCC'C Cuts at: 2496 2496 Size: 6370 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 3679 3905 4057 6166 1493 Size: 2186 226 152 2109 1697 Fragments arranged by size: 2186 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 2046 3695 1870 Size: 73 103 1649 4545 Fragments arranged by size: 4545 1649 103 73 BamHI G'GATC_C Cuts at: 2939 3700 4067 2939 Size: 761 367 5242 Fragments arranged by size: 5242 761 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 2792 3450 3691 5146 Size: 325 217 627 404 601 658 241 1455 Cuts at: 5146 618 Size: 1842 Fragments arranged by size: 1842 1455 658 627 601 404 325 241 217 BanII G_rGCy'C Cuts at: 1391 2426 2496 3292 3689 1391 Size: 1035 70 796 397 4072 Fragments arranged by size: 4072 1035 796 397 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 3668 928 Size: 971 221 1548 3630 Fragments arranged by size: 3630 1548 971 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4561 4595 1903 Size: 34 2624 34 3678 Fragments arranged by size: 3678 2624 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 1548 Size: 6370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 4981 Size: 107 122 71 631 605 337 9 2963 Cuts at: 4981 136 Size: 1525 Fragments arranged by size: 2963 1525 631 605 337 122 107 71 9 BglII A'GATC_T Cuts at: 3621 6365 3621 Size: 2744 3626 BlpI GC'TnA_GC Cuts at: 3204 3787 3204 Size: 583 5787 Bme1580I G_kGCm'C Cuts at: 1790 2229 2496 3453 3600 4431 5587 1790 Size: 439 267 957 147 831 1156 2573 Fragments arranged by size: 2573 1156 957 831 439 267 147 BmgBI CAC'GTC Cuts at: 1318 3385 1318 Size: 2067 4303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2746 2915 4101 Size: 416 53 83 399 24 1329 169 1186 Cuts at: 4101 5059 442 Size: 958 1753 Fragments arranged by size: 1753 1329 1186 958 416 399 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2679 2679 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2860 3021 3650 3667 5030 1239 Size: 1621 161 629 17 1363 2579 Fragments arranged by size: 2579 1621 1363 629 161 17 Bpu10I CC'TnA_GC Cuts at: 2991 2991 Size: 6370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 4495 5360 5658 2651 Size: 1844 865 298 3363 Fragments arranged by size: 3363 1844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 3626 5033 882 Size: 617 618 1509 1407 2219 Fragments arranged by size: 2219 1509 1407 618 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 2986 4161 493 Size: 711 1236 546 1175 2702 Fragments arranged by size: 2702 1236 1175 711 546 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3443 4177 4559 5194 Size: 53 83 186 936 1910 734 382 635 Cuts at: 5194 275 Size: 1451 Fragments arranged by size: 1910 1451 936 734 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4803 5201 5475 5622 6033 4803 Size: 398 274 147 411 5140 Fragments arranged by size: 5140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2345 2375 1178 Size: 30 165 30 942 30 5173 Fragments arranged by size: 5173 942 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2366 2446 3515 1186 Size: 1177 3 80 1069 4041 Fragments arranged by size: 4041 1177 1069 80 3 BseYI C'CCAG_C Cuts at: 2092 2935 3069 3210 5573 6069 2092 Size: 843 134 141 2363 496 2393 Fragments arranged by size: 2393 2363 843 496 141 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5797 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3820 4581 5609 1540 Size: 275 186 12 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2970 4431 4516 5587 2970 Size: 1461 85 1071 3753 Fragments arranged by size: 3753 1461 1071 85 BsmI GAATG_Cn' Cuts at: 2173 2788 3881 3974 2173 Size: 615 1093 93 4569 Fragments arranged by size: 4569 1093 615 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2426 2496 2970 3292 3453 3600 Size: 399 439 197 70 474 322 161 147 Cuts at: 3600 3689 4431 4516 5587 1391 Size: 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 439 399 322 197 161 147 89 85 70 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3725 844 Size: 2881 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2104 4257 1394 Size: 429 281 2153 3507 Fragments arranged by size: 3507 2153 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 2844 4865 5039 65 Size: 1183 1596 2021 174 1396 Fragments arranged by size: 2021 1596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3276 5014 5201 1700 Size: 110 1466 1738 187 2869 Fragments arranged by size: 2869 1738 1466 187 110 BsrGI T'GTAC_A Cuts at: 96 3012 3399 96 Size: 2916 387 3067 Fragments arranged by size: 3067 2916 387 BssHII G'CGCG_C Cuts at: 1925 2056 1925 Size: 131 6239 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6370 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6370 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2159 2092 Size: 67 6303 BstYI r'GATC_y Cuts at: 1972 2939 3621 3700 4067 4452 4469 5921 6007 Size: 967 682 79 367 385 17 1452 86 Cuts at: 6007 6019 6365 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 967 682 385 367 346 86 79 17 12 Bsu36I CC'TnA_GG Cuts at: 2406 2406 Size: 6370 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 513 Size: 552 939 114 4765 Fragments arranged by size: 4765 939 552 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 2377 2514 3886 4680 4700 2377 Size: 137 1372 794 20 4047 Fragments arranged by size: 4047 1372 794 137 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3640 5377 809 Size: 507 2324 1737 1802 Fragments arranged by size: 2324 1802 1737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2082 2551 Size: 54 1425 50 275 18 180 72 469 Cuts at: 2551 3042 3309 3817 4706 6335 8 Size: 491 267 508 889 1629 43 Fragments arranged by size: 1629 1425 889 508 491 469 275 267 180 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3817 1537 Size: 275 198 1807 4090 Fragments arranged by size: 4090 1807 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2648 3429 3584 4300 1353 Size: 719 576 781 155 716 3423 Fragments arranged by size: 3423 781 719 716 576 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2171 4957 5331 5477 387 Size: 1064 138 582 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 582 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2680 2860 3021 3650 3667 4433 Size: 393 527 521 180 161 629 17 766 Cuts at: 4433 5030 5817 6085 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 766 629 597 527 521 393 268 180 161 17 EcoNI CCTnn'n_nnAGG Cuts at: 2329 2630 2329 Size: 301 6069 EcoO109I rG'GnC_Cy Cuts at: 2356 3239 2356 Size: 883 5487 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 EcoRV GAT'ATC Cuts at: 2445 2445 Size: 6370 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2692 2269 Size: 423 5947 FspI TGC'GCA Cuts at: 4876 4876 Size: 6370 HaeII r_GCGC'y Cuts at: 1678 2649 2764 3036 5517 1678 Size: 971 115 272 2481 2531 Fragments arranged by size: 2531 2481 971 272 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2705 2737 3374 Size: 32 135 32 773 32 328 32 637 Cuts at: 3374 3406 3428 3460 5011 5043 5085 5117 1373 Size: 32 22 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 773 637 328 135 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3097 3714 3960 669 Size: 988 281 1159 617 246 3079 Fragments arranged by size: 3079 1159 988 617 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5484 5668 1305 Size: 4179 184 2007 Fragments arranged by size: 4179 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 3044 3311 10 Size: 54 1425 343 252 960 267 3069 Fragments arranged by size: 3069 1425 960 343 267 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2261 4328 4687 4846 518 Size: 648 1038 57 2067 359 159 2042 Fragments arranged by size: 2067 2042 1038 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2798 3010 3069 3214 3786 4463 5611 5856 Size: 1192 212 59 145 572 677 1148 245 Cuts at: 5856 1606 Size: 2120 Fragments arranged by size: 2120 1192 1148 677 572 245 212 145 59 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4879 Fragments arranged by size: 4879 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 2010 3817 2010 Size: 1807 4563 NspI r_CATG'y Cuts at: 1132 1229 2896 3728 5273 1132 Size: 97 1667 832 1545 2229 Fragments arranged by size: 2229 1667 1545 832 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6370 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PfoI T'CCnGG_A Cuts at: 3627 3644 3627 Size: 17 6353 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PspOMI G'GGCC_C Cuts at: 2492 2492 Size: 6370 PstI C_TGCA'G Cuts at: 830 3722 830 Size: 2892 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 3214 3214 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 4618 6330 4618 Size: 1712 4658 SfcI C'TryA_G Cuts at: 826 1045 3408 3718 4853 5534 5725 826 Size: 219 2363 310 1135 681 191 1471 Fragments arranged by size: 2363 1471 1135 681 310 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 2048 3697 1872 Size: 176 1649 4545 Fragments arranged by size: 4545 1649 176 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 4474 5375 5637 820 Size: 197 926 723 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 723 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2324 2537 1517 Size: 807 213 5350 Fragments arranged by size: 5350 807 213 StyI C'CwwG_G Cuts at: 513 1065 2004 3809 513 Size: 552 939 1805 3074 Fragments arranged by size: 3074 1805 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3282 4397 4414 4567 4752 5197 325 Size: 1255 1702 1115 17 153 185 445 1498 Fragments arranged by size: 1702 1498 1255 1115 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3012 3399 4616 Size: 274 80 33 51 1443 1035 387 1217 Cuts at: 4616 6328 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1217 1035 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6330 6362 6330 Size: 32 6338 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6370 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2637 2884 2637 Size: 247 6123 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 2046 3695 1870 Size: 176 1649 4545 Fragments arranged by size: 4545 1649 176 XmnI GAAnn'nnTTC Cuts at: 4499 4499 Size: 6370 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI