Restriction map for pFN21AB5503
 (Linear) (Six-base) MAPSORT of: KIBB5503.seq  Check: 4300  from: 1  to: 1665



KIBB5503.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     728    1665 
   Size:        728     937
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     675    1665 
   Size:        154     521     990

  Fragments arranged by size: 

                 990     521     154
 
AfeI AGC'GCT

Cuts at:      0     642    1665 
   Size:        642    1023
 
AhdI GACnn_n'nnGTC

Cuts at:      0     714    1665 
   Size:        714     951
 
AleI CACnn'nnGTG

Cuts at:      0     199     256    1665 
   Size:        199      57    1409

  Fragments arranged by size: 

                1409     199      57
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1344    1665 
   Size:       1344     321
 
ApaI G_GGCC'C

Cuts at:      0     491    1665 
   Size:        491    1174
 
AvaI C'yCGr_G

Cuts at:      0      41    1665 
   Size:         41    1624
 
BamHI G'GATC_C

Cuts at:      0     934    1665 
   Size:        934     731
 
BanI G'GyrC_C

Cuts at:      0     186     787    1445    1665 
   Size:        186     601     658     220

  Fragments arranged by size: 

                 658     601     220     186
 
BanII G_rGCy'C

Cuts at:      0     421     491    1287    1665 
   Size:        421      70     796     378

  Fragments arranged by size: 

                 796     421     378      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     115    1663    1665 
   Size:        115    1548       2

  Fragments arranged by size: 

                1548     115       2
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13    1665 
   Size:         13    1652
 
BglII A'GATC_T

Cuts at:      0    1616    1665 
   Size:       1616      49
 
BlpI GC'TnA_GC

Cuts at:      0    1199    1665 
   Size:       1199     466
 
Bme1580I G_kGCm'C

Cuts at:      0     224     491    1448    1595    1665 
   Size:        224     267     957     147      70

  Fragments arranged by size: 

                 957     267     224     147      70
 
BmgBI CAC'GTC

Cuts at:      0    1380    1665 
   Size:       1380     285
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     741     910    1665 
   Size:        741     169     755

  Fragments arranged by size: 

                 755     741     169
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     674    1665 
   Size:        674     991
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     855    1016    1645    1662    1665 
   Size:        855     161     629      17       3

  Fragments arranged by size: 

                 855     629     161      17       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     986    1665 
   Size:        986     679
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646    1665 
   Size:        646    1019
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     112    1621    1665 
   Size:        112    1509      44

  Fragments arranged by size: 

                1509     112      44
 
BsaAI yAC'GTr

Cuts at:      0     435     981    1665 
   Size:        435     546     684

  Fragments arranged by size: 

                 684     546     435
 
BsaHI Gr'CG_yC

Cuts at:      0    1438    1665 
   Size:       1438     227
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     340     370    1665 
   Size:        340      30    1295

  Fragments arranged by size: 

                1295     340      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     361     441    1510    1665 
   Size:        358       3      80    1069     155

  Fragments arranged by size: 

                1069     358     155      80       3
 
BseYI C'CCAG_C

Cuts at:      0      87     930    1064    1205    1665 
   Size:         87     843     134     141     460

  Fragments arranged by size: 

                 843     460     141     134      87
 
BsiEI CG_ry'CG

Cuts at:      0       8    1665 
   Size:          8    1657
 
BsiHKAI G_wGCw'C

Cuts at:      0     965    1665 
   Size:        965     700
 
BsmI GAATG_Cn'

Cuts at:      0     168     783    1665 
   Size:        168     615     882

  Fragments arranged by size: 

                 882     615     168
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     421     491     965    1287    1448    1595    1665 
   Size:        224     197      70     474     322     161     147      70

  Fragments arranged by size: 

                 474     322     224     197     161     147      70      70
            
 
BsrBI CCG'CTC

Cuts at:      0      99    1665 
   Size:         99    1566
 
BsrDI GCAATG_nn'

Cuts at:      0     839    1665 
   Size:        839     826
 
BsrFI r'CCGG_y

Cuts at:      0    1271    1665 
   Size:       1271     394
 
BsrGI T'GTAC_A

Cuts at:      0    1007    1394    1665 
   Size:       1007     387     271

  Fragments arranged by size: 

                1007     387     271
 
BssHII G'CGCG_C

Cuts at:      0      51    1665 
   Size:         51    1614
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      87     154    1665 
   Size:         87      67    1511

  Fragments arranged by size: 

                1511      87      67
 
BstYI r'GATC_y

Cuts at:      0     934    1616    1665 
   Size:        934     682      49

  Fragments arranged by size: 

                 934     682      49
 
Bsu36I CC'TnA_GG

Cuts at:      0     401    1665 
   Size:        401    1264
 
BtgI C'CryG_G

Cuts at:      0     113    1665 
   Size:        113    1552
 
BtsI GCAGTG_nn'

Cuts at:      0     372     509    1665 
   Size:        372     137    1156

  Fragments arranged by size: 

                1156     372     137
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1635    1665 
   Size:       1635      30
 
EaeI y'GGCC_r

Cuts at:      0       5      77     546    1037    1304    1665 
   Size:          5      72     469     491     267     361

  Fragments arranged by size: 

                 491     469     361     267      72       5
 
EagI C'GGCC_G

Cuts at:      0       5    1665 
   Size:          5    1660
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     643    1424    1579    1665 
   Size:         67     576     781     155      86

  Fragments arranged by size: 

                 781     576     155      86      67
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     166    1665 
   Size:        166    1499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     675     855    1016    1645    1662    1665 
   Size:        154     521     180     161     629      17       3

  Fragments arranged by size: 

                 629     521     180     161     154      17       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     324     625    1665 
   Size:        324     301    1040

  Fragments arranged by size: 

                1040     324     301
 
EcoO109I rG'GnC_Cy

Cuts at:      0     351    1234    1665 
   Size:        351     883     431

  Fragments arranged by size: 

                 883     431     351
 
EcoRV GAT'ATC

Cuts at:      0     440    1665 
   Size:        440    1225
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264     687    1665 
   Size:        264     423     978

  Fragments arranged by size: 

                 978     423     264
 
HaeII r_GCGC'y

Cuts at:      0     644     759    1031    1665 
   Size:        644     115     272     634

  Fragments arranged by size: 

                 644     634     272     115
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372     700     732    1369    1401    1423    1455 
   Size:        340      32     328      32     637      32      22      32

Cuts at:   1455    1665 
   Size:        210

  Fragments arranged by size: 

                 637     340     328     210      32      32      32      32
                  22
 
HincII GTy'rAC

Cuts at:      0    1092    1665 
   Size:       1092     573
 
MscI TGG'CCA

Cuts at:      0      79    1039    1306    1665 
   Size:         79     960     267     359

  Fragments arranged by size: 

                 960     359     267      79
 
MslI CAynn'nnrTG

Cuts at:      0     199     256    1665 
   Size:        199      57    1409

  Fragments arranged by size: 

                1409     199      57
 
MspA1I CmG'CkG

Cuts at:      0     793    1005    1064    1209    1665 
   Size:        793     212      59     145     456

  Fragments arranged by size: 

                 793     456     212     145      59
 
NotI GC'GGCC_GC

Cuts at:      0       5    1665 
   Size:          5    1660
 
NspI r_CATG'y

Cuts at:      0     891    1665 
   Size:        891     774
 
PfoI T'CCnGG_A

Cuts at:      0    1622    1639    1665 
   Size:       1622      17      26

  Fragments arranged by size: 

                1622      26      17
 
PspOMI G'GGCC_C

Cuts at:      0     487    1665 
   Size:        487    1178
 
PvuII CAG'CTG

Cuts at:      0    1209    1665 
   Size:       1209     456
 
SfcI C'TryA_G

Cuts at:      0    1403    1665 
   Size:       1403     262
 
SmaI CCC'GGG

Cuts at:      0      43    1665 
   Size:         43    1622
 
SmlI C'TyrA_G

Cuts at:      0     661    1665 
   Size:        661    1004
 
StuI AGG'CCT

Cuts at:      0     319     532    1665 
   Size:        319     213    1133

  Fragments arranged by size: 

                1133     319     213
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1277    1665 
   Size:       1277     388
 
TatI w'GTAC_w

Cuts at:      0    1007    1394    1665 
   Size:       1007     387     271

  Fragments arranged by size: 

                1007     387     271
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     632     879    1665 
   Size:        632     247     786

  Fragments arranged by size: 

                 786     632     247
 
XmaI C'CCGG_G

Cuts at:      0      41    1665 
   Size:         41    1624
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AhdI     AleI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbsI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstXI    BstYI   Bsu36I
     BtgI     BtsI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII     MscI
     MslI   MspA1I     NotI     NspI     PfoI   PspOMI    PvuII     SfcI
     SmaI     SmlI     StuI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AlfI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BmtI    BsaBI
    BsaWI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BssSI
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII  EcoICRI    EcoRI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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