Restriction map for
pFN21AB5443
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseRI
BsgI BsiWI BsmFI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstF5I BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FokI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MamI
MboII MfeI MluI MluNI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 361 GTCTAC GT^[AC][GT]AC
AccIII 556 TCCGGA T^CCGGA
AcyI 7 GGCGTC G[GA]^CG[CT]C
AlwI 329 GGATC GGATC(4/5)
ApaI 48 GGGCCC GGGCC^C
AspHI 204 GAGCAC G[AT]GC[AT]^C
AvaI 446 CCCGGG C^[CT]CG[GA]G
BbrPI 349 CACGTG CAC^GTG
BcgI 305 GCACTTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 245 TGATCA T^GATCA
BfaI 268 CTAG C^TAG
BglI 178 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 349 CACGTG [CT]AC^GT[GA]
BsaHI 7 GGCGTC G[GA]^CG[CT]C
BsaWI 556 TCCGGA [AT]^CCGG[AT]
BseAI 556 TCCGGA T^CCGGA
BsiEI 492 CGACCG CG[GA][CT]^CG
BsiHKAI 204 GAGCAC G[AT]GC[AT]^C
BsmAI 70 GAGAC GTCTC(1/5)
BsmBI 70 GAGACG CGTCTC(1/5)
BsoBI 446 CCCGGG C^[CT]CG[GA]G
BspEI 556 TCCGGA T^CCGGA
BsrBI 514 GAGCGG CCGCTC(-3/-3)
BsrFI 408 GCCGGT [GA]^CCGG[CT]
BssSI 214 CACGAG CACGAG(-5/-1)
BstEII 430 GGTGACC G^GT[ACGT]ACC
BstPI 430 GGTGACC G^GT[ACGT]ACC
BstXI 80 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 329 GGATCT [GA]^GATC[CT]
Cfr10I 408 GCCGGT [GA]^CCGG[CT]
EaeI 406 TGGCCG [CT]^GGCC[GA]
EcoO65I 430 GGTGACC G^GT[ACGT]ACC
EcoT14I 179 CCAAGG C^C[AT][AT]GG
FbaI 245 TGATCA T^GATCA
HaeII 175 AGCGCC [GA]GCGC^[CT]
HgaI 8 GCGTC GACGC(5/10)
Hin1I 7 GGCGTC G[GA]^CG[CT]C
KspI 57 CCGCGG CCGC^GG
MaeI 268 CTAG C^TAG
MflI 329 GGATCT [GA]^GATC[CT]
MroI 556 TCCGGA T^CCGGA
MseI 341 TTAA T^TAA
MspA1I 57 CCGCGG C[AC]G^C[GT]G
PmaCI 349 CACGTG CAC^GTG
PmlI 349 CACGTG CAC^GTG
SacII 57 CCGCGG CCGC^GG
ScaI 542 AGTACT AGT^ACT
SexAI 260 ACCAGGT A^CC[AT]GGT
SfaNI 155 GCATC GCATC(5/9)
SfcI 333 CTACAG C^T[GA][CT]AG
SmaI 446 CCCGGG CCC^GGG
SmlI 505 CTCAAG C^T[CT][GA]AG
SpeI 267 ACTAGT A^CTAGT
StyI 179 CCAAGG C^C[AT][AT]GG
Tru9I 341 TTAA T^TAA
TspRI 144 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 329 GGATCT [GA]^GATC[CT]
XmaCI 446 CCCGGG C^CCGGG
XmaI 446 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 58 CGCG CG^CG
320 CGCG
AfaI 265 GTAC GT^AC
543 GTAC
AluI 17 AGCT AG^CT
509 AGCT
BanII 48 GGGCCC G[GA]GC[CT]^C
76 GAGCCC
BstUI 58 CGCG CG^CG
320 CGCG
DpnII 246 GATC ^GATC
330 GATC
DsaI 57 CCGCGG C^C[GA][CT]GG
137 CCACGG
MaeIII 431 GTGAC ^GT[ACGT]AC
442 GTGAC
MboI 246 GATC ^GATC
330 GATC
MslI 156 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
378 CATACAGGTG
MvnI 58 CGCG CG^CG
320 CGCG
NdeII 246 GATC ^GATC
330 GATC
NlaIII 243 CATG CATG^
393 CATG
NlaIV 48 GGGCCC GG[ACGT]^[ACGT]CC
75 GGAGCC
PleI 115 GACTC GAGTC(4/5)
467 GAGTC
RsaI 265 GTAC GT^AC
543 GTAC
Sau3AI 246 GATC ^GATC
330 GATC
TaqI 257 TCGA T^CGA
547 TCGA
TfiI 292 GATTC G^A[AT]TC
560 GATTC
Tsp45I 431 GTGAC ^GT[GC]AC
442 GTGAC
TthHB8I 257 TCGA T^CGA
547 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MnlI MspI MvaI MwoI
NciI Sau96I ScrFI TseI