Restriction map for pFN21AB5443
 (Circular) (Six-base) MAPSORT of: pFN21AB5443.seq  Check: 7683  from: 1  to: 5284



pFN21AB5443  5284 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:31  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3094     278 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2367    2627    1937 
   Size:        430     260    4594

  Fragments arranged by size: 

                4594     430     260
 
Acc65I G'GTAC_C

Cuts at:   2605    2605 
   Size:       5284
 
AclI AA'CG_TT

Cuts at:   1563    3411    3784    1563 
   Size:       1848     373    3063

  Fragments arranged by size: 

                3063    1848     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3347    4731    4999    1632 
   Size:       1715    1384     268    1917

  Fragments arranged by size: 

                1917    1715    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5087
 
AflIII A'CryG_T

Cuts at:   1203    4183    5160    1203 
   Size:       2980     977    1327

  Fragments arranged by size: 

                2980    1327     977
 
AgeI A'CCGG_T

Cuts at:   4115    4115 
   Size:       5284
 
AhdI GACnn_n'nnGTC

Cuts at:   4013    4013 
   Size:       5284
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2183    1184 
   Size:        999    4285
 
AlwNI CAG_nnn'CTG

Cuts at:   3027    4599    3027 
   Size:       1572    3712
 
ApaI G_GGCC'C

Cuts at:   2057    2057 
   Size:       5284
 
ApaLI G'TGCA_C

Cuts at:   3341    4497    3341 
   Size:       1156    4128
 
ApoI r'AATT_y

Cuts at:   1493    2593    2819    2971    5080    1493 
   Size:       1100     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1100     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3838     160 
   Size:        872    2806    1606

  Fragments arranged by size: 

                2806    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2451    2609    1870 
   Size:         73     508     158    4545

  Fragments arranged by size: 

                4545     508     158      73
 
BamHI G'GATC_C

Cuts at:   2614    2981    2614 
   Size:        367    4917
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2605    4060     618 
   Size:        325     217     627     818    1455    1842

  Fragments arranged by size: 

                1842    1455     818     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2057    2085    2603    1391 
   Size:        666      28     518    4072

  Fragments arranged by size: 

                4072     666     518      28
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4313
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    2299    2333    3475    3509    1903 
   Size:         34      50      34     278      34    1142      34    3678

  Fragments arranged by size: 

                3678    1142     278      50      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3176    4386    3176 
   Size:       1210    4074
 
BclI T'GATC_A

Cuts at:   1548    2250    1548 
   Size:        702    4582
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2189    3895     136 
   Size:        107     122      71     631     605     517    1706    1525

  Fragments arranged by size: 

                1706    1525     631     605     517     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5279    5279 
   Size:       5284
 
BlpI GC'TnA_GC

Cuts at:   2701    2701 
   Size:       5284
 
Bme1580I G_kGCm'C

Cuts at:   1790    2057    2150    3345    4501    1790 
   Size:        267      93    1195    1156    2573

  Fragments arranged by size: 

                2573    1195    1156     267      93
 
BmgBI CAC'GTC

Cuts at:   1318    2133    2446    1318 
   Size:        815     313    4156

  Fragments arranged by size: 

                4156     815     313
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3015    3973     442 
   Size:        416      53      83     399      24    1598     958    1753

  Fragments arranged by size: 

                1753    1598     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5284
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2525    2525 
   Size:       5284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2039    2114    2537    3944    1239 
   Size:        800      75     423    1407    2579

  Fragments arranged by size: 

                2579    1407     800     423      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2495    3409    4274    4572    2495 
   Size:        914     865     298    3207

  Fragments arranged by size: 

                3207     914     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3947     882 
   Size:        617    2448    2219

  Fragments arranged by size: 

                2448    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2356    3075     493 
   Size:        711    1152     719    2702

  Fragments arranged by size: 

                2702    1152     719     711
 
BsaBI GATnn'nnATC

Cuts at:   5278    5278 
   Size:       5284
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2013    3091    3473    4108 
   Size:         53      83     186     936     480    1078     382     635

Cuts at:   4108     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1078     936     635     480     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2561    3717    4115    4389    4536    4947    2561 
   Size:       1156     398     274     147     411    2898

  Fragments arranged by size: 

                2898    1156     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2087    2117    1178 
   Size:         30     165      30     684      30    4345

  Fragments arranged by size: 

                4345     684     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5284
 
BseYI C'CCAG_C

Cuts at:   2008    2032    4487    4983    2008 
   Size:         24    2455     496    2309

  Fragments arranged by size: 

                2455    2309     496      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4711
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2500    2734    3495    4523    1540 
   Size:        275     186     499     234     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     499     275     234     186
 
BsiHKAI G_wGCw'C

Cuts at:   2213    3345    3430    4501    2213 
   Size:       1132      85    1071    2996

  Fragments arranged by size: 

                2996    1132    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2795    2888    2795 
   Size:         93    5191
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2069    2069 
   Size:       5284
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2057    2085    2150    2213    2603    3345    3430 
   Size:        399     267      28      65      63     390     742      85

Cuts at:   3430    4501    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     390     267      85      65
                  63      28
 
BspEI T'CCGG_A

Cuts at:   2561    4947    2561 
   Size:       2386    2898
 
BspHI T'CATG_A

Cuts at:   3173    4903    3173 
   Size:       1730    3554
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2639     844 
   Size:       1795    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2521    3171    1394 
   Size:        429     698     650    3507

  Fragments arranged by size: 

                3507     698     650     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3779    3953      65 
   Size:       1183    2531     174    1396

  Fragments arranged by size: 

                2531    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2413    3928    4115    1700 
   Size:        110     603    1515     187    2869

  Fragments arranged by size: 

                2869    1515     603     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5284
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5284
 
BssSI C'ACGA_G

Cuts at:   2219    3344    4356    2219 
   Size:       1125    1012    3147

  Fragments arranged by size: 

                3147    1125    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5284
 
BstBI TT'CG_AA

Cuts at:   4088    4088 
   Size:       5284
 
BstEII G'GTnAC_C

Cuts at:   1184    2435    1184 
   Size:       1251    4033
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2092    2092 
   Size:       5284
 
BstYI r'GATC_y

Cuts at:   1972    2334    2614    2981    3366    3383    4835    4921    4933 
   Size:        362     280     367     385      17    1452      86      12

Cuts at:   4933    5279    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     362     346     280      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2062    2142     513 
   Size:        552     997      80    3655

  Fragments arranged by size: 

                3655     997     552      80
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2302     487 
   Size:        683      56    1076    3469

  Fragments arranged by size: 

                3469    1076     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2148    2800    3594    3614    2148 
   Size:        652     794      20    3818

  Fragments arranged by size: 

                3818     794     652      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5284
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5249
 
DraI TTT'AAA

Cuts at:   2588    2588 
   Size:       5284
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4291     809 
   Size:        507    2975    1802

  Fragments arranged by size: 

                2975    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2411    2731    3620 
   Size:         54    1425      50     275      18     581     320     889

Cuts at:   3620    5249       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     581     320     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2731    1537 
   Size:        275     919    4090

  Fragments arranged by size: 

                4090     919     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3214    1353 
   Size:       1861    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3871    4245    4391     387 
   Size:       1064     138    2282     374     146    1280

  Fragments arranged by size: 

                2282    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2039    2114    2537    3347    3944    4731    4999 
   Size:        393     407      75     423     810     597     787     268

Cuts at:   4999    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     810     787     597     423     407     393     268
                  75
 
EcoRI G'AATT_C

Cuts at:   2593    2971    2593 
   Size:        378    4906
 
FspI TGC'GCA

Cuts at:   3790    3790 
   Size:       5284
 
HaeII r_GCGC'y

Cuts at:   1678    2184    4431    1678 
   Size:        506    2247    2531

  Fragments arranged by size: 

                2531    2247     506
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2224    2256    2463    2495    3925 
   Size:         32     135      32     652      32     207      32    1430

Cuts at:   3925    3957    3999    4031    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1430     652     207     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2628    2874     669 
   Size:        988     281     690     246    3079

  Fragments arranged by size: 

                3079     988     690     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5284
 
HpaI GTT'AAC

Cuts at:   2874    2874 
   Size:       5284
 
KpnI G_GTAC'C

Cuts at:   2609    2609 
   Size:       5284
 
MfeI C'AATT_G

Cuts at:   2883    2883 
   Size:       5284
 
MluI A'CGCG_T

Cuts at:   5160    5160 
   Size:       5284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2148    2160    4398    4582    1305 
   Size:        843      12    2238     184    2007

  Fragments arranged by size: 

                2238    2007     843     184      12
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3462

  Fragments arranged by size: 

                3462    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2165    2387    3242    3601    3760     518 
   Size:        648     999     222     855     359     159    2042

  Fragments arranged by size: 

                2042     999     855     648     359     222     159
 
MspA1I CmG'CkG

Cuts at:   1606    2064    2700    3377    4525    4770    1606 
   Size:        458     636     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     636     458     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4732
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5284
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5284
 
NotI GC'GGCC_GC

Cuts at:   2731    2731 
   Size:       5284
 
NspI r_CATG'y

Cuts at:   1132    1229    2642    4187    1132 
   Size:         97    1413    1545    2229

  Fragments arranged by size: 

                2229    1545    1413      97
 
PasI CC'CwG_GG

Cuts at:   1348    2155    1348 
   Size:        807    4477
 
PciI A'CATG_T

Cuts at:   4183    4183 
   Size:       5284
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5284
 
PfoI T'CCnGG_A

Cuts at:   2016    2016 
   Size:       5284
 
PmeI GTTT'AAAC

Cuts at:   2588    2588 
   Size:       5284
 
PmlI CAC'GTG

Cuts at:   2356    2356 
   Size:       5284
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3353    3385    4866    4898    3353 
   Size:         32    1481      32    3739

  Fragments arranged by size: 

                3739    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5284
 
PsiI TTA'TAA

Cuts at:   2854    2854 
   Size:       5284
 
PspOMI G'GGCC_C

Cuts at:   2053    2053 
   Size:       5284
 
PstI C_TGCA'G

Cuts at:    830    2636     830 
   Size:       1806    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5284
 
SacII CC_GC'GG

Cuts at:   2065    2065 
   Size:       5284
 
SalI G'TCGA_C

Cuts at:   1936    2626    1936 
   Size:        690    4594
 
SbfI CC_TGCA'GG

Cuts at:   2636    2636 
   Size:       5284
 
ScaI AGT'ACT

Cuts at:   2549    3532    5244    2549 
   Size:        983    1712    2589

  Fragments arranged by size: 

                2589    1712     983
 
SexAI A'CCwGG_T

Cuts at:   2265    2265 
   Size:       5284
 
SfcI C'TryA_G

Cuts at:    826    1045    2338    2632    3767    4448    4639     826 
   Size:        219    1293     294    1135     681     191    1471

  Fragments arranged by size: 

                1471    1293    1135     681     294     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5284
 
SmaI CCC'GGG

Cuts at:   1872    2453    2611    1872 
   Size:        581     158    4545

  Fragments arranged by size: 

                4545     581     158
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2510    3388    4289    4551     820 
   Size:        197     926     567     878     901     262    1553

  Fragments arranged by size: 

                1553     926     901     878     567     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3075     493 
   Size:       2582    2702
 
SpeI A'CTAG_T

Cuts at:    152    2272     152 
   Size:       2120    3164
 
SphI G_CATG'C

Cuts at:   1132    2642    1132 
   Size:       1510    3774
 
SspI AAT'ATT

Cuts at:      5      52    3208       5 
   Size:         47    3156    2081

  Fragments arranged by size: 

                3156    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5284
 
StyI C'CwwG_G

Cuts at:    513    1065    2184    2723     513 
   Size:        552    1119     539    3074

  Fragments arranged by size: 

                3074    1119     552     539
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2514    3311    3328    3481    3666    4111     325 
   Size:       1255     934     797      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     934     797     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2547    3530    5242 
   Size:        274      80      33      51    1443     570     983    1712

Cuts at:   5242      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443     983     570     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2087    2119    2210    2242    2556    2588    5244    5276    2087 
   Size:         32      91      32     314      32    2656      32    2095

  Fragments arranged by size: 

                2656    2095     314      91      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5284
 
XbaI T'CTAG_A

Cuts at:   2620    2620 
   Size:       5284
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5284
 
XmaI C'CCGG_G

Cuts at:   1870    2451    2609    1870 
   Size:        581     158    4545

  Fragments arranged by size: 

                4545     581     158
 
XmnI GAAnn'nnTTC

Cuts at:   3413    3413 
   Size:       5284
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3092     276 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PstI     PvuI    SacII     SalI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI    PpuMI    PspXI     PsrI    PvuII
    RsrII     SacI    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
     XcmI
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