(Circular) (Six-base) MAPSORT of: pFN21AB5443.seq Check: 7683 from: 1 to: 5284 pFN21AB5443 5284 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3094 278 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2367 2627 1937 Size: 430 260 4594 Fragments arranged by size: 4594 430 260 Acc65I G'GTAC_C Cuts at: 2605 2605 Size: 5284 AclI AA'CG_TT Cuts at: 1563 3411 3784 1563 Size: 1848 373 3063 Fragments arranged by size: 3063 1848 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 4731 4999 1632 Size: 1715 1384 268 1917 Fragments arranged by size: 1917 1715 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5087 AflIII A'CryG_T Cuts at: 1203 4183 5160 1203 Size: 2980 977 1327 Fragments arranged by size: 2980 1327 977 AgeI A'CCGG_T Cuts at: 4115 4115 Size: 5284 AhdI GACnn_n'nnGTC Cuts at: 4013 4013 Size: 5284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2183 1184 Size: 999 4285 AlwNI CAG_nnn'CTG Cuts at: 3027 4599 3027 Size: 1572 3712 ApaI G_GGCC'C Cuts at: 2057 2057 Size: 5284 ApaLI G'TGCA_C Cuts at: 3341 4497 3341 Size: 1156 4128 ApoI r'AATT_y Cuts at: 1493 2593 2819 2971 5080 1493 Size: 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 226 152 AseI AT'TA_AT Cuts at: 160 1032 3838 160 Size: 872 2806 1606 Fragments arranged by size: 2806 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 AvaI C'yCGr_G Cuts at: 1870 1943 2451 2609 1870 Size: 73 508 158 4545 Fragments arranged by size: 4545 508 158 73 BamHI G'GATC_C Cuts at: 2614 2981 2614 Size: 367 4917 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2605 4060 618 Size: 325 217 627 818 1455 1842 Fragments arranged by size: 1842 1455 818 627 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2085 2603 1391 Size: 666 28 518 4072 Fragments arranged by size: 4072 666 518 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2299 2333 3475 3509 1903 Size: 34 50 34 278 34 1142 34 3678 Fragments arranged by size: 3678 1142 278 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3176 4386 3176 Size: 1210 4074 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 4582 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2189 3895 136 Size: 107 122 71 631 605 517 1706 1525 Fragments arranged by size: 1706 1525 631 605 517 122 107 71 BglII A'GATC_T Cuts at: 5279 5279 Size: 5284 BlpI GC'TnA_GC Cuts at: 2701 2701 Size: 5284 Bme1580I G_kGCm'C Cuts at: 1790 2057 2150 3345 4501 1790 Size: 267 93 1195 1156 2573 Fragments arranged by size: 2573 1195 1156 267 93 BmgBI CAC'GTC Cuts at: 1318 2133 2446 1318 Size: 815 313 4156 Fragments arranged by size: 4156 815 313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3015 3973 442 Size: 416 53 83 399 24 1598 958 1753 Fragments arranged by size: 1753 1598 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2525 2525 Size: 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2114 2537 3944 1239 Size: 800 75 423 1407 2579 Fragments arranged by size: 2579 1407 800 423 75 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 3409 4274 4572 2495 Size: 914 865 298 3207 Fragments arranged by size: 3207 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3947 882 Size: 617 2448 2219 Fragments arranged by size: 2448 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2356 3075 493 Size: 711 1152 719 2702 Fragments arranged by size: 2702 1152 719 711 BsaBI GATnn'nnATC Cuts at: 5278 5278 Size: 5284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 3091 3473 4108 Size: 53 83 186 936 480 1078 382 635 Cuts at: 4108 275 Size: 1451 Fragments arranged by size: 1451 1078 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2561 3717 4115 4389 4536 4947 2561 Size: 1156 398 274 147 411 2898 Fragments arranged by size: 2898 1156 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2087 2117 1178 Size: 30 165 30 684 30 4345 Fragments arranged by size: 4345 684 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5284 BseYI C'CCAG_C Cuts at: 2008 2032 4487 4983 2008 Size: 24 2455 496 2309 Fragments arranged by size: 2455 2309 496 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4711 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2500 2734 3495 4523 1540 Size: 275 186 499 234 761 1028 2301 Fragments arranged by size: 2301 1028 761 499 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2213 3345 3430 4501 2213 Size: 1132 85 1071 2996 Fragments arranged by size: 2996 1132 1071 85 BsmI GAATG_Cn' Cuts at: 2795 2888 2795 Size: 93 5191 BsmBI CGTCTCn'nnnn_ Cuts at: 2069 2069 Size: 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2085 2150 2213 2603 3345 3430 Size: 399 267 28 65 63 390 742 85 Cuts at: 3430 4501 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 267 85 65 63 28 BspEI T'CCGG_A Cuts at: 2561 4947 2561 Size: 2386 2898 BspHI T'CATG_A Cuts at: 3173 4903 3173 Size: 1730 3554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2639 844 Size: 1795 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2521 3171 1394 Size: 429 698 650 3507 Fragments arranged by size: 3507 698 650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3779 3953 65 Size: 1183 2531 174 1396 Fragments arranged by size: 2531 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2413 3928 4115 1700 Size: 110 603 1515 187 2869 Fragments arranged by size: 2869 1515 603 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5284 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5284 BssSI C'ACGA_G Cuts at: 2219 3344 4356 2219 Size: 1125 1012 3147 Fragments arranged by size: 3147 1125 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5284 BstBI TT'CG_AA Cuts at: 4088 4088 Size: 5284 BstEII G'GTnAC_C Cuts at: 1184 2435 1184 Size: 1251 4033 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2092 Size: 5284 BstYI r'GATC_y Cuts at: 1972 2334 2614 2981 3366 3383 4835 4921 4933 Size: 362 280 367 385 17 1452 86 12 Cuts at: 4933 5279 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 362 346 280 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2062 2142 513 Size: 552 997 80 3655 Fragments arranged by size: 3655 997 552 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2302 487 Size: 683 56 1076 3469 Fragments arranged by size: 3469 1076 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2800 3594 3614 2148 Size: 652 794 20 3818 Fragments arranged by size: 3818 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5249 DraI TTT'AAA Cuts at: 2588 2588 Size: 5284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4291 809 Size: 507 2975 1802 Fragments arranged by size: 2975 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2411 2731 3620 Size: 54 1425 50 275 18 581 320 889 Cuts at: 3620 5249 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 581 320 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 1537 Size: 275 919 4090 Fragments arranged by size: 4090 919 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3214 1353 Size: 1861 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3871 4245 4391 387 Size: 1064 138 2282 374 146 1280 Fragments arranged by size: 2282 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2114 2537 3347 3944 4731 4999 Size: 393 407 75 423 810 597 787 268 Cuts at: 4999 1239 Size: 1524 Fragments arranged by size: 1524 810 787 597 423 407 393 268 75 EcoRI G'AATT_C Cuts at: 2593 2971 2593 Size: 378 4906 FspI TGC'GCA Cuts at: 3790 3790 Size: 5284 HaeII r_GCGC'y Cuts at: 1678 2184 4431 1678 Size: 506 2247 2531 Fragments arranged by size: 2531 2247 506 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 2463 2495 3925 Size: 32 135 32 652 32 207 32 1430 Cuts at: 3925 3957 3999 4031 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1430 652 207 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2874 669 Size: 988 281 690 246 3079 Fragments arranged by size: 3079 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5284 HpaI GTT'AAC Cuts at: 2874 2874 Size: 5284 KpnI G_GTAC'C Cuts at: 2609 2609 Size: 5284 MfeI C'AATT_G Cuts at: 2883 2883 Size: 5284 MluI A'CGCG_T Cuts at: 5160 5160 Size: 5284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 2160 4398 4582 1305 Size: 843 12 2238 184 2007 Fragments arranged by size: 2238 2007 843 184 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3462 Fragments arranged by size: 3462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2387 3242 3601 3760 518 Size: 648 999 222 855 359 159 2042 Fragments arranged by size: 2042 999 855 648 359 222 159 MspA1I CmG'CkG Cuts at: 1606 2064 2700 3377 4525 4770 1606 Size: 458 636 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 636 458 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4732 NdeI CA'TA_TG Cuts at: 387 387 Size: 5284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5284 NotI GC'GGCC_GC Cuts at: 2731 2731 Size: 5284 NspI r_CATG'y Cuts at: 1132 1229 2642 4187 1132 Size: 97 1413 1545 2229 Fragments arranged by size: 2229 1545 1413 97 PasI CC'CwG_GG Cuts at: 1348 2155 1348 Size: 807 4477 PciI A'CATG_T Cuts at: 4183 4183 Size: 5284 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5284 PfoI T'CCnGG_A Cuts at: 2016 2016 Size: 5284 PmeI GTTT'AAAC Cuts at: 2588 2588 Size: 5284 PmlI CAC'GTG Cuts at: 2356 2356 Size: 5284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3385 4866 4898 3353 Size: 32 1481 32 3739 Fragments arranged by size: 3739 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5284 PsiI TTA'TAA Cuts at: 2854 2854 Size: 5284 PspOMI G'GGCC_C Cuts at: 2053 2053 Size: 5284 PstI C_TGCA'G Cuts at: 830 2636 830 Size: 1806 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5284 SacII CC_GC'GG Cuts at: 2065 2065 Size: 5284 SalI G'TCGA_C Cuts at: 1936 2626 1936 Size: 690 4594 SbfI CC_TGCA'GG Cuts at: 2636 2636 Size: 5284 ScaI AGT'ACT Cuts at: 2549 3532 5244 2549 Size: 983 1712 2589 Fragments arranged by size: 2589 1712 983 SexAI A'CCwGG_T Cuts at: 2265 2265 Size: 5284 SfcI C'TryA_G Cuts at: 826 1045 2338 2632 3767 4448 4639 826 Size: 219 1293 294 1135 681 191 1471 Fragments arranged by size: 1471 1293 1135 681 294 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5284 SmaI CCC'GGG Cuts at: 1872 2453 2611 1872 Size: 581 158 4545 Fragments arranged by size: 4545 581 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 3388 4289 4551 820 Size: 197 926 567 878 901 262 1553 Fragments arranged by size: 1553 926 901 878 567 262 197 SnaBI TAC'GTA Cuts at: 493 3075 493 Size: 2582 2702 SpeI A'CTAG_T Cuts at: 152 2272 152 Size: 2120 3164 SphI G_CATG'C Cuts at: 1132 2642 1132 Size: 1510 3774 SspI AAT'ATT Cuts at: 5 52 3208 5 Size: 47 3156 2081 Fragments arranged by size: 3156 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5284 StyI C'CwwG_G Cuts at: 513 1065 2184 2723 513 Size: 552 1119 539 3074 Fragments arranged by size: 3074 1119 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 3311 3328 3481 3666 4111 325 Size: 1255 934 797 17 153 185 445 1498 Fragments arranged by size: 1498 1255 934 797 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 3530 5242 Size: 274 80 33 51 1443 570 983 1712 Cuts at: 5242 96 Size: 138 Fragments arranged by size: 1712 1443 983 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2210 2242 2556 2588 5244 5276 2087 Size: 32 91 32 314 32 2656 32 2095 Fragments arranged by size: 2656 2095 314 91 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5284 XbaI T'CTAG_A Cuts at: 2620 2620 Size: 5284 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5284 XmaI C'CCGG_G Cuts at: 1870 2451 2609 1870 Size: 581 158 4545 Fragments arranged by size: 4545 581 158 XmnI GAAnn'nnTTC Cuts at: 3413 3413 Size: 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3092 276 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI