Restriction map for
pFN21AB5443
(Linear) (Six-base) MAPSORT of: KIBB5443.seq Check: 355 from: 1 to: 579
KIBB5443.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 362 579
Size: 362 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 579
Size: 178 401
ApaI G_GGCC'C
Cuts at: 0 52 579
Size: 52 527
AvaI C'yCGr_G
Cuts at: 0 446 579
Size: 446 133
BanII G_rGCy'C
Cuts at: 0 52 80 579
Size: 52 28 499
Fragments arranged by size:
499 52 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 328 579
Size: 294 34 251
Fragments arranged by size:
294 251 34
BclI T'GATC_A
Cuts at: 0 245 579
Size: 245 334
BglI GCCn_nnn'nGGC
Cuts at: 0 184 579
Size: 184 395
Bme1580I G_kGCm'C
Cuts at: 0 52 145 579
Size: 52 93 434
Fragments arranged by size:
434 93 52
BmgBI CAC'GTC
Cuts at: 0 128 441 579
Size: 128 313 138
Fragments arranged by size:
313 138 128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 520 579
Size: 520 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 109 532 579
Size: 34 75 423 47
Fragments arranged by size:
423 75 47 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 579
Size: 490 89
BsaAI yAC'GTr
Cuts at: 0 351 579
Size: 351 228
BsaHI Gr'CG_yC
Cuts at: 0 8 579
Size: 8 571
BsaWI w'CCGG_w
Cuts at: 0 556 579
Size: 556 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 579
Size: 82 30 467
Fragments arranged by size:
467 82 30
BseYI C'CCAG_C
Cuts at: 0 3 27 579
Size: 3 24 552
Fragments arranged by size:
552 24 3
BsiEI CG_ry'CG
Cuts at: 0 495 579
Size: 495 84
BsiHKAI G_wGCw'C
Cuts at: 0 208 579
Size: 208 371
BsmBI CGTCTCn'nnnn_
Cuts at: 0 64 579
Size: 64 515
Bsp1286I G_dGCh'C
Cuts at: 0 52 80 145 208 579
Size: 52 28 65 63 371
Fragments arranged by size:
371 65 63 52 28
BspEI T'CCGG_A
Cuts at: 0 556 579
Size: 556 23
BsrBI CCG'CTC
Cuts at: 0 516 579
Size: 516 63
BsrFI r'CCGG_y
Cuts at: 0 408 579
Size: 408 171
BssSI C'ACGA_G
Cuts at: 0 214 579
Size: 214 365
BstEII G'GTnAC_C
Cuts at: 0 430 579
Size: 430 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 579
Size: 87 492
BstYI r'GATC_y
Cuts at: 0 329 579
Size: 329 250
BtgI C'CryG_G
Cuts at: 0 57 137 579
Size: 57 80 442
Fragments arranged by size:
442 80 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 297 579
Size: 297 282
BtsI GCAGTG_nn'
Cuts at: 0 143 579
Size: 143 436
EaeI y'GGCC_r
Cuts at: 0 406 579
Size: 406 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 109 532 579
Size: 34 75 423 47
Fragments arranged by size:
423 75 47 34
HaeII r_GCGC'y
Cuts at: 0 179 579
Size: 179 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 458 490 579
Size: 219 32 207 32 89
Fragments arranged by size:
219 207 89 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 155 579
Size: 143 12 424
Fragments arranged by size:
424 143 12
MslI CAynn'nnrTG
Cuts at: 0 160 382 579
Size: 160 222 197
Fragments arranged by size:
222 197 160
MspA1I CmG'CkG
Cuts at: 0 59 579
Size: 59 520
PasI CC'CwG_GG
Cuts at: 0 150 579
Size: 150 429
PfoI T'CCnGG_A
Cuts at: 0 11 579
Size: 11 568
PmlI CAC'GTG
Cuts at: 0 351 579
Size: 351 228
PspOMI G'GGCC_C
Cuts at: 0 48 579
Size: 48 531
SacII CC_GC'GG
Cuts at: 0 60 579
Size: 60 519
ScaI AGT'ACT
Cuts at: 0 544 579
Size: 544 35
SexAI A'CCwGG_T
Cuts at: 0 260 579
Size: 260 319
SfcI C'TryA_G
Cuts at: 0 333 579
Size: 333 246
SmaI CCC'GGG
Cuts at: 0 448 579
Size: 448 131
SmlI C'TyrA_G
Cuts at: 0 505 579
Size: 505 74
SpeI A'CTAG_T
Cuts at: 0 267 579
Size: 267 312
StyI C'CwwG_G
Cuts at: 0 179 579
Size: 179 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 579
Size: 509 70
TatI w'GTAC_w
Cuts at: 0 542 579
Size: 542 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 205 237 551 579
Size: 82 32 91 32 314 28
Fragments arranged by size:
314 91 82 32 32 28
XmaI C'CCGG_G
Cuts at: 0 446 579
Size: 446 133
Enzymes that do cut:
AccI AlfI ApaI AvaI BanII BcgI BclI BglI
Bme1580I BmgBI BplI BpmI BpuEI BsaAI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI
BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
EaeI Eco57MI HaeII Hin4I MmeI MslI MspA1I PasI
PfoI PmlI PspOMI SacII ScaI SexAI SfcI SmaI
SmlI SpeI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BciVI BfrBI BglII BlpI BmrI BmtI Bpu10I
BsaI BsaBI BseRI BsgI BsiWI BsmI BspHI BspMI
BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmnI ZraI