(Linear) (Six-base) MAPSORT of: KIBB5419.seq  Check: 8522  from: 1  to: 954



KIBB5419.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     533     954 
   Size:        533     421
 
Acc65I G'GTAC_C

Cuts at:      0     774     954 
   Size:        774     180
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     954 
   Size:        522     432
 
ApoI r'AATT_y

Cuts at:      0      56     945     954 
   Size:         56     889       9

  Fragments arranged by size: 

                 889      56       9
 
BanI G'GyrC_C

Cuts at:      0     774     954 
   Size:        774     180
 
BsaBI GATnn'nnATC

Cuts at:      0     559     954 
   Size:        559     395
 
BseYI C'CCAG_C

Cuts at:      0     121     954 
   Size:        121     833
 
BspHI T'CATG_A

Cuts at:      0     512     954 
   Size:        512     442
 
BsrDI GCAATG_nn'

Cuts at:      0     173     954 
   Size:        173     781
 
BsrGI T'GTAC_A

Cuts at:      0     791     954 
   Size:        791     163
 
BstEII G'GTnAC_C

Cuts at:      0     360     954 
   Size:        360     594
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     315     954 
   Size:        315     639
 
BstZ17I GTA'TAC

Cuts at:      0     534     954 
   Size:        534     420
 
BtgI C'CryG_G

Cuts at:      0      50     954 
   Size:         50     904
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     294     954 
   Size:        294     660
 
BtsI GCAGTG_nn'

Cuts at:      0      14     954 
   Size:         14     940
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     687     954 
   Size:        687     267
 
EaeI y'GGCC_r

Cuts at:      0      29     203     305     954 
   Size:         29     174     102     649

  Fragments arranged by size: 

                 649     174     102      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     954 
   Size:        522     432
 
EcoRI G'AATT_C

Cuts at:      0     945     954 
   Size:        945       9
 
HindIII A'AGCT_T

Cuts at:      0     337     954 
   Size:        337     617
 
KpnI G_GTAC'C

Cuts at:      0     778     954 
   Size:        778     176
 
MscI TGG'CCA

Cuts at:      0      31     205     307     954 
   Size:         31     174     102     647

  Fragments arranged by size: 

                 647     174     102      31
 
MslI CAynn'nnrTG

Cuts at:      0      49     103     313     476     517     954 
   Size:         49      54     210     163      41     437

  Fragments arranged by size: 

                 437     210     163      54      49      41
 
MspA1I CmG'CkG

Cuts at:      0     245     954 
   Size:        245     709
 
NcoI C'CATG_G

Cuts at:      0      50     954 
   Size:         50     904
 
PasI CC'CwG_GG

Cuts at:      0     390     954 
   Size:        390     564
 
PstI C_TGCA'G

Cuts at:      0     131     954 
   Size:        131     823
 
PvuII CAG'CTG

Cuts at:      0     245     954 
   Size:        245     709
 
SfcI C'TryA_G

Cuts at:      0     127     240     954 
   Size:        127     113     714

  Fragments arranged by size: 

                 714     127     113
 
StyI C'CwwG_G

Cuts at:      0      50     954 
   Size:         50     904
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     335     954 
   Size:        335     619
 
TatI w'GTAC_w

Cuts at:      0     791     954 
   Size:        791     163
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApoI     BanI    BsaBI    BseYI    BspHI
    BsrDI    BsrGI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     DrdI     EaeI  Eco57MI    EcoRI  HindIII     KpnI     MscI     MslI
   MspA1I     NcoI     PasI     PstI    PvuII     SfcI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI   Bsu36I     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     MfeI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI