Restriction map for
pFN21AB5407
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwI 878 GGATC GGATC(4/5)
AviII 884 TGCGCA TGC^GCA
BalI 782 TGGCCA TGG^CCA
BbsI 1009 GTCTTC GAAGAC(2/6)
BglI 862 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1009 GTCTTC GAAGAC(2/6)
BsaAI 799 TACGTG [CT]AC^GT[GA]
BsiEI 467 CGGCCG CG[GA][CT]^CG
BspHI 761 TCATGA T^CATGA
BsrFI 1397 GCCGGC [GA]^CCGG[CT]
BssSI 1273 CACGAG CACGAG(-5/-1)
Cfr10I 1397 GCCGGC [GA]^CCGG[CT]
EagI 467 CGGCCG C^GGCCG
EarI 721 CTCTTC CTCTTC(1/4)
EclXI 467 CGGCCG C^GGCCG
Eco52I 467 CGGCCG C^GGCCG
EcoNI 1437 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1031 CCATGG C^C[AT][AT]GG
FspI 884 TGCGCA TGC^GCA
HincII 79 GTCAAC GT[CT]^[GA]AC
HindII 79 GTCAAC GT[CT]^[GA]AC
HinfI 526 GAGTC G^A[ACGT]TC
Ksp632I 721 CTCTTC CTCTTC(1/4)
KspI 1262 CCGCGG CCGC^GG
MluNI 782 TGGCCA TGG^CCA
MscI 782 TGGCCA TGG^CCA
MseI 1061 TTAA T^TAA
NaeI 1397 GCCGGC GCC^GGC
NcoI 1031 CCATGG C^CATGG
NgoMIV 1397 GCCGGC G^CCGGC
PleI 526 GAGTC GAGTC(4/5)
PshAI 1104 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 761 TCATGA T^CATGA
SacII 1262 CCGCGG CCGC^GG
StyI 1031 CCATGG C^C[AT][AT]GG
TaqI 269 TCGA T^CGA
Tru9I 1061 TTAA T^TAA
TthHB8I 269 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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BbeI 482 GGCGCC GGCGC^C
1380 GGCGCC
BsmBI 579 CGTCTC CGTCTC(1/5)
676 GAGACG
BsmFI 113 GGGAC GGGAC(10/14)
242 GGGAC
BsrI 969 ACTGG ACTGG(1/-1)
1074 CCAGT
BssHII 424 GCGCGC G^CGCGC
892 GCGCGC
BstAPI 858 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
867 GCAGGCGCTGC
DpnII 420 GATC ^GATC
879 GATC
KasI 482 GGCGCC G^GCGCC
1380 GGCGCC
MaeIII 729 GTCAC ^GT[ACGT]AC
1340 GTGAC
MboI 420 GATC ^GATC
879 GATC
NarI 482 GGCGCC GG^CGCC
1380 GGCGCC
NdeII 420 GATC ^GATC
879 GATC
SacI 45 GAGCTC GAGCT^C
151 GAGCTC
Sau3AI 420 GATC ^GATC
879 GATC
SfoI 482 GGCGCC GGC^GCC
1380 GGCGCC
SmlI 358 CTCAAG C^T[CT][GA]AG
949 CTCAAG
Tsp45I 729 GTCAC ^GT[GC]AC
1340 GTGAC
TspRI 995 CAGTG CA[GC]TG[ACGT][ACGT]^
1338 CAGTG
XcmI 1133 CCAAGACCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1268 CCAAGCACGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI PstI RsaI Sau96I ScrFI
SfaNI SfcI TseI