(Circular) (Six-base) MAPSORT of: pFN21AB5407.seq Check: 5503 from: 1 to: 6187 pFN21AB5407 6187 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2095 2617 2095 Size: 522 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 4314 4687 1563 Size: 2751 373 3063 Fragments arranged by size: 3063 2751 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3428 4250 5634 5902 1632 Size: 1796 822 1384 268 1917 Fragments arranged by size: 1917 1796 1384 822 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5990 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 2829 1166 Size: 1663 4524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2847 1184 Size: 1663 4524 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2351 2383 2351 Size: 32 6155 AlwNI CAG_nnn'CTG Cuts at: 2110 2632 2878 3371 3930 5502 2110 Size: 522 246 493 559 1572 2795 Fragments arranged by size: 2795 1572 559 522 493 246 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 3496 3722 3874 5983 1493 Size: 2003 226 152 2109 1697 Fragments arranged by size: 2109 2003 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 2528 2618 2926 3512 1870 Size: 73 585 90 308 586 4545 Fragments arranged by size: 4545 586 585 308 90 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2487 2609 2831 3385 3508 Size: 325 217 627 700 122 222 554 123 Cuts at: 3508 4963 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 700 627 554 325 222 217 123 122 BanII G_rGCy'C Cuts at: 1391 2054 2160 3338 3506 1391 Size: 663 106 1178 168 4072 Fragments arranged by size: 4072 1178 663 168 106 BbeI G_GCGC'C Cuts at: 2491 3389 2491 Size: 898 5289 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3007 928 Size: 971 1108 4108 Fragments arranged by size: 4108 1108 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4378 4412 1903 Size: 34 2441 34 3678 Fragments arranged by size: 3678 2441 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4079 5289 4079 Size: 1210 4977 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2873 4798 136 Size: 107 122 71 631 605 1201 1925 1525 Fragments arranged by size: 1925 1525 1201 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 3604 3604 Size: 6187 Bme1580I G_kGCm'C Cuts at: 1790 2543 2612 2836 4248 5404 1790 Size: 753 69 224 1412 1156 2573 Fragments arranged by size: 2573 1412 1156 753 224 69 BmgBI CAC'GTC Cuts at: 1318 2782 1318 Size: 1464 4723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2984 3073 3918 Size: 416 53 83 399 24 1567 89 845 Cuts at: 3918 4876 442 Size: 958 1753 Fragments arranged by size: 1753 1567 958 845 416 399 89 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2438 2540 2690 2729 3281 3289 3305 3348 Size: 1199 102 150 39 552 8 16 43 Cuts at: 3348 3458 4847 1239 Size: 110 1389 2579 Fragments arranged by size: 2579 1389 1199 552 150 110 102 43 39 16 8 Bpu10I CC'TnA_GC Cuts at: 2396 2396 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 2939 4312 5177 5475 2348 Size: 591 1373 865 298 3060 Fragments arranged by size: 3060 1373 865 591 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 3050 3257 4850 882 Size: 617 636 915 207 1593 2219 Fragments arranged by size: 2219 1593 915 636 617 207 BsaAI yAC'GTr Cuts at: 493 1204 2806 3978 493 Size: 711 1602 1172 2702 Fragments arranged by size: 2702 1602 1172 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2488 2637 3386 Size: 53 83 186 936 723 232 149 749 Cuts at: 3386 3994 4376 5011 275 Size: 608 382 635 1451 Fragments arranged by size: 1451 936 749 723 635 608 382 232 186 149 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 4663 Fragments arranged by size: 4663 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2426 2435 2914 3464 1186 Size: 928 312 9 479 550 3909 Fragments arranged by size: 3909 928 550 479 312 9 BseYI C'CCAG_C Cuts at: 2174 3148 5390 5886 2174 Size: 974 2242 496 2475 Fragments arranged by size: 2475 2242 974 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 2732 2879 2891 1163 Size: 573 723 273 147 12 4459 Fragments arranged by size: 4459 723 573 273 147 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2475 3637 4398 5426 1540 Size: 275 186 474 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 474 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2160 2565 2661 4248 4333 5404 2054 Size: 106 405 96 1587 85 1071 2837 Fragments arranged by size: 2837 1587 1071 405 106 96 85 BsmI GAATG_Cn' Cuts at: 3698 3791 3698 Size: 93 6094 BsmBI CGTCTCn'nnnn_ Cuts at: 2590 2675 2590 Size: 85 6102 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2160 2543 2565 2612 2661 2836 Size: 399 264 106 383 22 47 49 175 Cuts at: 2836 3338 3506 4248 4333 5404 1391 Size: 502 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 399 383 264 175 168 106 85 49 47 22 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 2766 4076 5806 2766 Size: 1310 1730 3147 Fragments arranged by size: 3147 1730 1310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2617 2724 3542 844 Size: 1251 522 107 818 3489 Fragments arranged by size: 3489 1251 818 522 107 BsrBI CCG'CTC Cuts at: 1394 1823 4074 1394 Size: 429 2251 3507 Fragments arranged by size: 3507 2251 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3402 4831 5018 1700 Size: 110 1592 1429 187 2869 Fragments arranged by size: 2869 1592 1429 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6187 BssHII G'CGCG_C Cuts at: 1925 2429 2897 1925 Size: 504 468 5215 Fragments arranged by size: 5215 504 468 BssSI C'ACGA_G Cuts at: 3278 4247 5259 3278 Size: 969 1012 4206 Fragments arranged by size: 4206 1012 969 BstAPI GCAn_nnn'nTGC Cuts at: 794 2869 2878 794 Size: 2075 9 4103 Fragments arranged by size: 4103 2075 9 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstYI r'GATC_y Cuts at: 1972 3517 3884 4269 4286 5738 5824 5836 6182 Size: 1545 367 385 17 1452 86 12 346 Cuts at: 6182 1972 Size: 1977 Fragments arranged by size: 1977 1545 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 3036 3267 513 Size: 552 939 588 444 231 3433 Fragments arranged by size: 3433 939 588 552 444 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2878 3175 3181 487 Size: 683 56 1652 297 6 3493 Fragments arranged by size: 3493 1652 683 297 56 6 BtsI GCAGTG_nn' Cuts at: 3006 3349 3703 4497 4517 3006 Size: 343 354 794 20 4676 Fragments arranged by size: 4676 794 354 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6152 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 2556 2787 Size: 54 1425 50 275 18 642 84 231 Cuts at: 2787 3165 3270 3634 4523 6152 8 Size: 378 105 364 889 1629 43 Fragments arranged by size: 1629 1425 889 642 378 364 275 231 105 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2472 3634 1537 Size: 275 660 1162 4090 Fragments arranged by size: 4090 1162 660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2732 4117 1353 Size: 1379 1385 3423 Fragments arranged by size: 3423 1385 1379 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 4774 5148 5294 387 Size: 1064 138 579 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 579 374 146 138 EcoICRI GAG'CTC Cuts at: 2052 2158 2052 Size: 106 6081 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2540 2690 2729 3281 3289 3305 Size: 393 806 102 150 39 552 8 16 Cuts at: 3305 3348 3428 3458 4250 4847 5634 5902 1239 Size: 43 80 30 792 597 787 268 1524 Fragments arranged by size: 1524 806 792 787 597 552 393 268 150 102 80 43 39 30 16 8 EcoNI CCTnn'n_nnAGG Cuts at: 3446 3446 Size: 6187 EcoO109I rG'GnC_Cy Cuts at: 2119 2248 3465 2119 Size: 129 1217 4841 Fragments arranged by size: 4841 1217 129 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 2980 2767 Size: 213 5974 FspI TGC'GCA Cuts at: 2891 4693 2891 Size: 1802 4385 FspAI rTGC'GCAy Cuts at: 2891 2891 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 2491 2879 3389 5334 1678 Size: 813 388 510 1945 2531 Fragments arranged by size: 2531 1945 813 510 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4828 4860 4902 4934 1373 Size: 32 135 32 3256 32 42 32 2626 Fragments arranged by size: 3256 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2086 3531 3777 669 Size: 988 281 148 1445 246 3079 Fragments arranged by size: 3079 1445 988 281 246 148 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KasI G'GCGC_C Cuts at: 2487 3385 2487 Size: 898 5289 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5301 5485 1305 Size: 3996 184 2007 Fragments arranged by size: 3996 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2789 10 Size: 54 1425 343 957 3408 Fragments arranged by size: 3408 1425 957 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2603 2829 3104 4145 4504 4663 Size: 648 1110 327 226 275 1041 359 159 Cuts at: 4663 518 Size: 2042 Fragments arranged by size: 2042 1110 1041 648 359 327 275 226 159 MspA1I CmG'CkG Cuts at: 1606 2057 2484 2493 3269 3312 3603 4280 5428 Size: 451 427 9 776 43 291 677 1148 Cuts at: 5428 5673 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 776 677 451 427 291 245 43 9 NaeI GCC'GGC Cuts at: 3404 3404 Size: 6187 NarI GG'CG_CC Cuts at: 2488 3386 2488 Size: 898 5289 NcoI C'CATG_G Cuts at: 513 1065 2004 3036 513 Size: 552 939 1032 3664 Fragments arranged by size: 3664 1032 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NgoMIV G'CCGG_C Cuts at: 3402 3402 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 3545 5090 1132 Size: 97 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3025 1348 Size: 1677 4510 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PfoI T'CCnGG_A Cuts at: 2031 2370 2448 2517 3258 2031 Size: 339 78 69 741 4960 Fragments arranged by size: 4960 741 339 78 69 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2248 3465 2119 Size: 129 1217 4841 Fragments arranged by size: 4841 1217 129 PshAI GACnn'nnGTC Cuts at: 1613 3113 1613 Size: 1500 4687 PsiI TTA'TAA Cuts at: 3757 3757 Size: 6187 PstI C_TGCA'G Cuts at: 830 2628 2937 3398 3539 830 Size: 1798 309 461 141 3478 Fragments arranged by size: 3478 1798 461 309 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 SacI G_AGCT'C Cuts at: 2054 2160 2054 Size: 106 6081 SacII CC_GC'GG Cuts at: 3270 3270 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SanDI GG'GwC_CC Cuts at: 2248 2248 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 4435 6147 4435 Size: 1712 4475 SfcI C'TryA_G Cuts at: 826 1045 2624 2933 3394 3535 4670 5351 5542 Size: 219 1579 309 461 141 1135 681 191 Cuts at: 5542 826 Size: 1471 Fragments arranged by size: 1579 1471 1135 681 461 309 219 191 141 SfoI GGC'GCC Cuts at: 2489 3387 2489 Size: 898 5289 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2363 2954 4291 5192 5454 820 Size: 197 926 420 591 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 591 420 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2004 3036 3626 513 Size: 552 939 1032 590 3074 Fragments arranged by size: 3074 1032 939 590 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2752 2819 3461 4214 4231 4384 4569 Size: 1255 1172 67 642 753 17 153 185 Cuts at: 4569 5014 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1172 753 642 445 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 4433 6145 Size: 274 80 33 51 1443 303 2153 1712 Cuts at: 6145 96 Size: 138 Fragments arranged by size: 2153 1712 1443 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2672 2704 6147 6179 2672 Size: 32 3443 32 2680 Fragments arranged by size: 3443 2680 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6187 XbaI T'CTAG_A Cuts at: 3523 3523 Size: 6187 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3145 3280 3145 Size: 135 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI