Restriction map for pFN21AB5407
 (Linear) (Six-base) MAPSORT of: KIBB5407.seq  Check: 6492  from: 1  to: 1482



KIBB5407.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      90     612    1482 
   Size:         90     522     870

  Fragments arranged by size: 

                 870     522      90
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1423    1482 
   Size:       1423      59
 
AleI CACnn'nnGTG

Cuts at:      0     824    1482 
   Size:        824     658
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     842    1482 
   Size:        842     640
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     346     378    1482 
   Size:        346      32    1104

  Fragments arranged by size: 

                1104     346      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     627     873    1366    1482 
   Size:        105     522     246     493     116

  Fragments arranged by size: 

                 522     493     246     116     105
 
AvaI C'yCGr_G

Cuts at:      0     523     613     921    1482 
   Size:        523      90     308     561

  Fragments arranged by size: 

                 561     523     308      90
 
BanI G'GyrC_C

Cuts at:      0     482     604     826    1380    1482 
   Size:        482     122     222     554     102

  Fragments arranged by size: 

                 554     482     222     122     102
 
BanII G_rGCy'C

Cuts at:      0      49     155    1333    1482 
   Size:         49     106    1178     149

  Fragments arranged by size: 

                1178     149     106      49
 
BbeI G_GCGC'C

Cuts at:      0     486    1384    1482 
   Size:        486     898      98

  Fragments arranged by size: 

                 898     486      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1002    1482 
   Size:       1002     480
 
BglI GCCn_nnn'nGGC

Cuts at:      0     868    1482 
   Size:        868     614
 
Bme1580I G_kGCm'C

Cuts at:      0     538     607     831    1482 
   Size:        538      69     224     651

  Fragments arranged by size: 

                 651     538     224      69
 
BmgBI CAC'GTC

Cuts at:      0     777    1482 
   Size:        777     705
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     979    1068    1482 
   Size:        979      89     414

  Fragments arranged by size: 

                 979     414      89
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     535     685     724    1276    1284    1300    1343 
   Size:        433     102     150      39     552       8      16      43

Cuts at:   1343    1453    1482 
   Size:        110      29

  Fragments arranged by size: 

                 552     433     150     110     102      43      39      29
                  16       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     391    1482 
   Size:        391    1091
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     934    1482 
   Size:        343     591     548

  Fragments arranged by size: 

                 591     548     343
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130    1045    1252    1482 
   Size:        130     915     207     230

  Fragments arranged by size: 

                 915     230     207     130
 
BsaAI yAC'GTr

Cuts at:      0     801    1482 
   Size:        801     681
 
BsaHI Gr'CG_yC

Cuts at:      0     251     483     632    1381    1482 
   Size:        251     232     149     749     101

  Fragments arranged by size: 

                 749     251     232     149     101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     667     697    1482 
   Size:        667      30     785

  Fragments arranged by size: 

                 785     667      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     421     430     909    1459    1482 
   Size:        109     312       9     479     550      23

  Fragments arranged by size: 

                 550     479     312     109      23       9
 
BseYI C'CCAG_C

Cuts at:      0     169    1143    1482 
   Size:        169     974     339

  Fragments arranged by size: 

                 974     339     169
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     727     874     886    1482 
   Size:        454     273     147      12     596

  Fragments arranged by size: 

                 596     454     273     147      12
 
BsiEI CG_ry'CG

Cuts at:      0     470    1482 
   Size:        470    1012
 
BsiHKAI G_wGCw'C

Cuts at:      0      49     155     560     656    1482 
   Size:         49     106     405      96     826

  Fragments arranged by size: 

                 826     405     106      96      49
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     585     670    1482 
   Size:        585      85     812

  Fragments arranged by size: 

                 812     585      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     155     538     560     607     656     831    1333 
   Size:         49     106     383      22      47      49     175     502

Cuts at:   1333    1482 
   Size:        149

  Fragments arranged by size: 

                 502     383     175     149     106      49      49      47
                  22
 
BspHI T'CATG_A

Cuts at:      0     761    1482 
   Size:        761     721
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      90     612     719    1482 
   Size:         90     522     107     763

  Fragments arranged by size: 

                 763     522     107      90
 
BsrFI r'CCGG_y

Cuts at:      0    1397    1482 
   Size:       1397      85
 
BssHII G'CGCG_C

Cuts at:      0     424     892    1482 
   Size:        424     468     590

  Fragments arranged by size: 

                 590     468     424
 
BssSI C'ACGA_G

Cuts at:      0    1273    1482 
   Size:       1273     209
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     864     873    1482 
   Size:        864       9     609

  Fragments arranged by size: 

                 864     609       9
 
BtgI C'CryG_G

Cuts at:      0     587    1031    1262    1482 
   Size:        587     444     231     220

  Fragments arranged by size: 

                 587     444     231     220
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     873    1170    1176    1482 
   Size:        873     297       6     306

  Fragments arranged by size: 

                 873     306     297       6
 
BtsI GCAGTG_nn'

Cuts at:      0    1001    1344    1482 
   Size:       1001     343     138

  Fragments arranged by size: 

                1001     343     138
 
EaeI y'GGCC_r

Cuts at:      0     467     551     782    1160    1265    1482 
   Size:        467      84     231     378     105     217

  Fragments arranged by size: 

                 467     378     231     217     105      84
 
EagI C'GGCC_G

Cuts at:      0     467    1482 
   Size:        467    1015
 
EarI CTCTTCn'nnn_

Cuts at:      0     727    1482 
   Size:        727     755
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     163    1482 
   Size:        163    1319
 
EcoICRI GAG'CTC

Cuts at:      0      47     153    1482 
   Size:         47     106    1329

  Fragments arranged by size: 

                1329     106      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     535     685     724    1276    1284    1300    1343 
   Size:        433     102     150      39     552       8      16      43

Cuts at:   1343    1423    1453    1482 
   Size:         80      30      29

  Fragments arranged by size: 

                 552     433     150     102      80      43      39      30
                  29      16       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1441    1482 
   Size:       1441      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     243    1460    1482 
   Size:        114     129    1217      22

  Fragments arranged by size: 

                1217     129     114      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     762     975    1482 
   Size:        762     213     507

  Fragments arranged by size: 

                 762     507     213
 
FspI TGC'GCA

Cuts at:      0     886    1482 
   Size:        886     596
 
FspAI rTGC'GCAy

Cuts at:      0     886    1482 
   Size:        886     596
 
HaeII r_GCGC'y

Cuts at:      0     486     874    1384    1482 
   Size:        486     388     510      98

  Fragments arranged by size: 

                 510     486     388      98
 
HincII GTy'rAC

Cuts at:      0      81    1482 
   Size:         81    1401
 
KasI G'GCGC_C

Cuts at:      0     482    1380    1482 
   Size:        482     898     102

  Fragments arranged by size: 

                 898     482     102
 
MscI TGG'CCA

Cuts at:      0     784    1482 
   Size:        784     698
 
MslI CAynn'nnrTG

Cuts at:      0     271     598     824    1099    1482 
   Size:        271     327     226     275     383

  Fragments arranged by size: 

                 383     327     275     271     226
 
MspA1I CmG'CkG

Cuts at:      0      52     479     488    1264    1307    1482 
   Size:         52     427       9     776      43     175

  Fragments arranged by size: 

                 776     427     175      52      43       9
 
NaeI GCC'GGC

Cuts at:      0    1399    1482 
   Size:       1399      83
 
NarI GG'CG_CC

Cuts at:      0     483    1381    1482 
   Size:        483     898     101

  Fragments arranged by size: 

                 898     483     101
 
NcoI C'CATG_G

Cuts at:      0    1031    1482 
   Size:       1031     451
 
NgoMIV G'CCGG_C

Cuts at:      0    1397    1482 
   Size:       1397      85
 
PasI CC'CwG_GG

Cuts at:      0    1020    1482 
   Size:       1020     462
 
PfoI T'CCnGG_A

Cuts at:      0      26     365     443     512    1253    1482 
   Size:         26     339      78      69     741     229

  Fragments arranged by size: 

                 741     339     229      78      69      26
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     243    1460    1482 
   Size:        114     129    1217      22

  Fragments arranged by size: 

                1217     129     114      22
 
PshAI GACnn'nnGTC

Cuts at:      0    1108    1482 
   Size:       1108     374
 
PstI C_TGCA'G

Cuts at:      0     623     932    1393    1482 
   Size:        623     309     461      89

  Fragments arranged by size: 

                 623     461     309      89
 
SacI G_AGCT'C

Cuts at:      0      49     155    1482 
   Size:         49     106    1327

  Fragments arranged by size: 

                1327     106      49
 
SacII CC_GC'GG

Cuts at:      0    1265    1482 
   Size:       1265     217
 
SanDI GG'GwC_CC

Cuts at:      0     243    1482 
   Size:        243    1239
 
SfcI C'TryA_G

Cuts at:      0     619     928    1389    1482 
   Size:        619     309     461      93

  Fragments arranged by size: 

                 619     461     309      93
 
SfoI GGC'GCC

Cuts at:      0     484    1382    1482 
   Size:        484     898     100

  Fragments arranged by size: 

                 898     484     100
 
SmlI C'TyrA_G

Cuts at:      0     358     949    1482 
   Size:        358     591     533

  Fragments arranged by size: 

                 591     533     358
 
StyI C'CwwG_G

Cuts at:      0    1031    1482 
   Size:       1031     451
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     747     814    1456    1482 
   Size:        747      67     642      26

  Fragments arranged by size: 

                 747     642      67      26
 
TatI w'GTAC_w

Cuts at:      0     275    1482 
   Size:        275    1207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     667     699    1482 
   Size:        667      32     783

  Fragments arranged by size: 

                 783     667      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1140    1275    1482 
   Size:       1140     135     207

  Fragments arranged by size: 

                1140     207     135
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    AlwNI     AvaI     BanI
    BanII     BbeI     BbsI     BglI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI
   BssHII    BssSI   BstAPI     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    FspAI    HaeII   HincII     KasI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     PasI     PfoI    PpuMI    PshAI     PstI
     SacI    SacII    SanDI     SfcI     SfoI     SmlI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI     BmtI     BplI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BsrBI    BsrDI    BsrGI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI    EcoRI    EcoRV
     FseI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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