(Circular) (Six-base) MAPSORT of: pFN21AB5366.seq Check: 9789 from: 1 to: 5338 pFN21AB5366 5338 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3148 278 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2681 1937 Size: 744 4594 Acc65I G'GTAC_C Cuts at: 2199 2659 2199 Size: 460 4878 AclI AA'CG_TT Cuts at: 1563 3465 3838 1563 Size: 1902 373 3063 Fragments arranged by size: 3063 1902 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2215 2375 2448 3401 4785 5053 1632 Size: 583 160 73 953 1384 268 1917 Fragments arranged by size: 1917 1384 953 583 268 160 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5141 AflIII A'CryG_T Cuts at: 1203 4237 5214 1203 Size: 3034 977 1327 Fragments arranged by size: 3034 1327 977 AgeI A'CCGG_T Cuts at: 2461 4169 2461 Size: 1708 3630 AhdI GACnn_n'nnGTC Cuts at: 4067 4067 Size: 5338 AleI CACnn'nnGTG Cuts at: 1166 2210 1166 Size: 1044 4294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5338 AlwNI CAG_nnn'CTG Cuts at: 2353 3081 4653 2353 Size: 728 1572 3038 Fragments arranged by size: 3038 1572 728 ApaLI G'TGCA_C Cuts at: 3395 4551 3395 Size: 1156 4182 ApoI r'AATT_y Cuts at: 1493 2527 2647 2873 3025 5134 1493 Size: 1034 120 226 152 2109 1697 Fragments arranged by size: 2109 1697 1034 226 152 120 AscI GG'CGCG_CC Cuts at: 2132 2132 Size: 5338 AseI AT'TA_AT Cuts at: 160 1032 3892 160 Size: 872 2860 1606 Fragments arranged by size: 2860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 AvaI C'yCGr_G Cuts at: 1870 1943 2502 2663 1870 Size: 73 559 161 4545 Fragments arranged by size: 4545 559 161 73 BamHI G'GATC_C Cuts at: 2299 2668 3035 2299 Size: 369 367 4602 Fragments arranged by size: 4602 369 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2199 2659 4114 618 Size: 325 217 627 221 191 460 1455 1842 Fragments arranged by size: 1842 1455 627 460 325 221 217 191 BanII G_rGCy'C Cuts at: 1391 2247 2657 1391 Size: 856 410 4072 Fragments arranged by size: 4072 856 410 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3529 3563 1903 Size: 34 1592 34 3678 Fragments arranged by size: 3678 1592 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3230 4440 3230 Size: 1210 4128 BclI T'GATC_A Cuts at: 1548 1548 Size: 5338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2022 3949 136 Size: 107 122 71 631 605 350 1927 1525 Fragments arranged by size: 1927 1525 631 605 350 122 107 71 BglII A'GATC_T Cuts at: 5333 5333 Size: 5338 BlpI GC'TnA_GC Cuts at: 2755 2755 Size: 5338 Bme1580I G_kGCm'C Cuts at: 1790 3399 4555 1790 Size: 1609 1156 2573 Fragments arranged by size: 2573 1609 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5338 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3069 4027 442 Size: 416 53 83 399 24 1652 958 1753 Fragments arranged by size: 1753 1652 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 5338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 3998 1239 Size: 1019 1740 2579 Fragments arranged by size: 2579 1740 1019 Bpu10I CC'TnA_GC Cuts at: 2246 2246 Size: 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3463 4328 4626 3463 Size: 865 298 4175 Fragments arranged by size: 4175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4001 882 Size: 617 2502 2219 Fragments arranged by size: 2502 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2076 3129 493 Size: 711 872 1053 2702 Fragments arranged by size: 2702 1053 872 711 BsaBI GATnn'nnATC Cuts at: 5332 5332 Size: 5338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3145 3527 4162 Size: 53 83 186 936 476 1136 382 635 Cuts at: 4162 275 Size: 1451 Fragments arranged by size: 1451 1136 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2456 2461 2475 3771 4169 4443 4590 5001 2456 Size: 5 14 1296 398 274 147 411 2793 Fragments arranged by size: 2793 1296 411 398 274 147 14 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5113 Fragments arranged by size: 5113 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2320 1186 Size: 1069 65 4204 Fragments arranged by size: 4204 1069 65 BseYI C'CCAG_C Cuts at: 2012 2448 4541 5037 2012 Size: 436 2093 496 2313 Fragments arranged by size: 2313 2093 496 436 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 1163 Size: 573 630 4135 Fragments arranged by size: 4135 630 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 2788 3549 4577 1540 Size: 275 186 223 564 761 1028 2301 Fragments arranged by size: 2301 1028 761 564 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2247 3399 3484 4555 2247 Size: 1152 85 1071 3030 Fragments arranged by size: 3030 1152 1071 85 BsmI GAATG_Cn' Cuts at: 2259 2849 2942 2259 Size: 590 93 4655 Fragments arranged by size: 4655 590 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2657 3399 3484 4555 1391 Size: 399 457 410 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 410 399 85 BspEI T'CCGG_A Cuts at: 2475 5001 2475 Size: 2526 2812 BspHI T'CATG_A Cuts at: 3227 4957 3227 Size: 1730 3608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2693 844 Size: 1849 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2367 3225 1394 Size: 429 544 858 3507 Fragments arranged by size: 3507 858 544 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3833 4007 65 Size: 1183 2585 174 1396 Fragments arranged by size: 2585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 2219 2255 2461 3982 4169 1700 Size: 110 279 130 36 206 1521 187 2869 Fragments arranged by size: 2869 1521 279 206 187 130 110 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5338 BssHII G'CGCG_C Cuts at: 1925 2132 2215 1925 Size: 207 83 5048 Fragments arranged by size: 5048 207 83 BssSI C'ACGA_G Cuts at: 3398 4410 3398 Size: 1012 4326 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5338 BstBI TT'CG_AA Cuts at: 4142 4142 Size: 5338 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5338 BstYI r'GATC_y Cuts at: 1972 2100 2299 2668 3035 3420 3437 4889 4975 Size: 128 199 369 367 385 17 1452 86 Cuts at: 4975 4987 5333 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 369 367 346 199 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2224 2322 513 Size: 552 939 186 34 98 3529 Fragments arranged by size: 3529 939 552 186 98 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 487 Size: 683 56 899 3700 Fragments arranged by size: 3700 899 683 56 BtsI GCAGTG_nn' Cuts at: 2854 3648 3668 2854 Size: 794 20 4524 Fragments arranged by size: 4524 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5303 DraI TTT'AAA Cuts at: 2642 2642 Size: 5338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4345 809 Size: 507 3029 1802 Fragments arranged by size: 3029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2221 2253 Size: 54 1425 50 275 18 240 151 32 Cuts at: 2253 2442 2785 3674 5303 8 Size: 189 343 889 1629 43 Fragments arranged by size: 1629 1425 889 343 275 240 189 151 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2221 2785 1537 Size: 275 409 564 4090 Fragments arranged by size: 4090 564 409 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2514 2582 3268 1353 Size: 1034 127 68 686 3423 Fragments arranged by size: 3423 1034 686 127 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2326 3925 4299 4445 387 Size: 1064 138 737 1599 374 146 1280 Fragments arranged by size: 1599 1280 1064 737 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2258 2375 2448 3401 3998 4785 Size: 393 583 43 117 73 953 597 787 Cuts at: 4785 5053 1239 Size: 268 1524 Fragments arranged by size: 1524 953 787 597 583 393 268 117 73 43 EcoO109I rG'GnC_Cy Cuts at: 2162 2162 Size: 5338 EcoRI G'AATT_C Cuts at: 2647 3025 2647 Size: 378 4960 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2059 2059 Size: 5338 FspI TGC'GCA Cuts at: 3844 3844 Size: 5338 HaeII r_GCGC'y Cuts at: 1678 2012 4485 1678 Size: 334 2473 2531 Fragments arranged by size: 2531 2473 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373 Size: 32 135 32 2407 32 42 32 2626 Fragments arranged by size: 2626 2407 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2682 2928 669 Size: 988 281 744 246 3079 Fragments arranged by size: 3079 988 744 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5338 HpaI GTT'AAC Cuts at: 2928 2928 Size: 5338 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5338 KpnI G_GTAC'C Cuts at: 2203 2663 2203 Size: 460 4878 MfeI C'AATT_G Cuts at: 2937 2937 Size: 5338 MluI A'CGCG_T Cuts at: 5214 5214 Size: 5338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4452 4636 1305 Size: 3147 184 2007 Fragments arranged by size: 3147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 2444 10 Size: 54 1425 343 240 372 2904 Fragments arranged by size: 2904 1425 372 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 2210 2294 3296 3655 3814 518 Size: 648 1044 84 1002 359 159 2042 Fragments arranged by size: 2042 1044 1002 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2062 2226 2350 2452 2754 3431 4579 4824 Size: 456 164 124 102 302 677 1148 245 Cuts at: 4824 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 456 302 245 164 124 102 NaeI GCC'GGC Cuts at: 2091 2221 2257 2091 Size: 130 36 5172 Fragments arranged by size: 5172 130 36 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5338 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3847 Fragments arranged by size: 3847 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5338 NgoMIV G'CCGG_C Cuts at: 2089 2219 2255 2089 Size: 130 36 5172 Fragments arranged by size: 5172 130 36 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5338 NotI GC'GGCC_GC Cuts at: 2785 2785 Size: 5338 NspI r_CATG'y Cuts at: 1132 1229 2696 4241 1132 Size: 97 1467 1545 2229 Fragments arranged by size: 2229 1545 1467 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5338 PciI A'CATG_T Cuts at: 4237 4237 Size: 5338 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5338 PmeI GTTT'AAAC Cuts at: 2642 2642 Size: 5338 PmlI CAC'GTG Cuts at: 2076 2076 Size: 5338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3407 3439 4920 4952 3407 Size: 32 1481 32 3793 Fragments arranged by size: 3793 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2162 2162 Size: 5338 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5338 PsiI TTA'TAA Cuts at: 2908 2908 Size: 5338 PspXI vC'TCGA_Gb Cuts at: 2502 2502 Size: 5338 PstI C_TGCA'G Cuts at: 830 2337 2690 830 Size: 1507 353 3478 Fragments arranged by size: 3478 1507 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5338 PvuII CAG'CTG Cuts at: 2452 2452 Size: 5338 RsrII CG'GwC_CG Cuts at: 2459 2459 Size: 5338 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5338 SacII CC_GC'GG Cuts at: 2227 2227 Size: 5338 SalI G'TCGA_C Cuts at: 1936 2680 1936 Size: 744 4594 SanDI GG'GwC_CC Cuts at: 2162 2162 Size: 5338 SapI GCTCTTCn'nnn_ Cuts at: 2582 2582 Size: 5338 SbfI CC_TGCA'GG Cuts at: 2337 2690 2337 Size: 353 4985 ScaI AGT'ACT Cuts at: 3586 5298 3586 Size: 1712 3626 SfcI C'TryA_G Cuts at: 826 1045 2333 2686 3821 4502 4693 826 Size: 219 1288 353 1135 681 191 1471 Fragments arranged by size: 1471 1288 1135 681 353 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5338 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5338 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5338 SmaI CCC'GGG Cuts at: 1872 2665 1872 Size: 793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2502 3442 4343 4605 820 Size: 197 926 559 940 901 262 1553 Fragments arranged by size: 1553 940 926 901 559 262 197 SnaBI TAC'GTA Cuts at: 493 3129 493 Size: 2636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5338 SphI G_CATG'C Cuts at: 1132 2696 1132 Size: 1564 3774 SspI AAT'ATT Cuts at: 5 52 3262 5 Size: 47 3210 2081 Fragments arranged by size: 3210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5338 StyI C'CwwG_G Cuts at: 513 1065 2004 2127 2601 2777 513 Size: 552 939 123 474 176 3074 Fragments arranged by size: 3074 939 552 474 176 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2462 3365 3382 3535 3720 4165 325 Size: 1255 882 903 17 153 185 445 1498 Fragments arranged by size: 1498 1255 903 882 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3584 5296 96 Size: 274 80 33 51 1443 1607 1712 138 Fragments arranged by size: 1712 1607 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5298 5330 5298 Size: 32 5306 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5338 XbaI T'CTAG_A Cuts at: 2674 2674 Size: 5338 XhoI C'TCGA_G Cuts at: 1943 2502 1943 Size: 559 4779 XmaI C'CCGG_G Cuts at: 1870 2663 1870 Size: 793 4545 XmnI GAAnn'nnTTC Cuts at: 2528 3467 2528 Size: 939 4399 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3146 276 Size: 53 83 186 936 1612 2468 Fragments arranged by size: 2468 1612 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI SexAI SfiI SrfI SwaI XcmI