Restriction map for pFN21AB5366
 (Linear) (Six-base) MAPSORT of: KIBB5366.seq  Check: 5171  from: 1  to: 633



KIBB5366.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

Acc65I G'GTAC_C

Cuts at:      0     194     633 
   Size:        194     439
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     370     443     633 
   Size:        210     160      73     190

  Fragments arranged by size: 

                 210     190     160      73
 
AgeI A'CCGG_T

Cuts at:      0     456     633 
   Size:        456     177
 
AleI CACnn'nnGTG

Cuts at:      0     205     633 
   Size:        205     428
 
AlwNI CAG_nnn'CTG

Cuts at:      0     348     633 
   Size:        348     285
 
ApoI r'AATT_y

Cuts at:      0     522     633 
   Size:        522     111
 
AscI GG'CGCG_CC

Cuts at:      0     127     633 
   Size:        127     506
 
AvaI C'yCGr_G

Cuts at:      0     497     633 
   Size:        497     136
 
BamHI G'GATC_C

Cuts at:      0     294     633 
   Size:        294     339
 
BanI G'GyrC_C

Cuts at:      0       3     194     633 
   Size:          3     191     439

  Fragments arranged by size: 

                 439     191       3
 
BanII G_rGCy'C

Cuts at:      0     242     633 
   Size:        242     391
 
BbeI G_GCGC'C

Cuts at:      0       7     633 
   Size:          7     626
 
BglI GCCn_nnn'nGGC

Cuts at:      0      17     633 
   Size:         17     616
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     378     633 
   Size:        378     255
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     633 
   Size:        253     380
 
Bpu10I CC'TnA_GC

Cuts at:      0     241     633 
   Size:        241     392
 
BsaAI yAC'GTr

Cuts at:      0      71     633 
   Size:         71     562
 
BsaHI Gr'CG_yC

Cuts at:      0       4     633 
   Size:          4     629
 
BsaWI w'CCGG_w

Cuts at:      0     451     456     470     633 
   Size:        451       5      14     163

  Fragments arranged by size: 

                 451     163      14       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     315     633 
   Size:        250      65     318

  Fragments arranged by size: 

                 318     250      65
 
BseYI C'CCAG_C

Cuts at:      0       7     443     633 
   Size:          7     436     190

  Fragments arranged by size: 

                 436     190       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     633 
   Size:        361     272
 
BsiEI CG_ry'CG

Cuts at:      0     219     633 
   Size:        219     414
 
BsiHKAI G_wGCw'C

Cuts at:      0     242     633 
   Size:        242     391
 
BsmI GAATG_Cn'

Cuts at:      0     254     633 
   Size:        254     379
 
Bsp1286I G_dGCh'C

Cuts at:      0     242     633 
   Size:        242     391
 
BspEI T'CCGG_A

Cuts at:      0     470     633 
   Size:        470     163
 
BsrBI CCG'CTC

Cuts at:      0     362     633 
   Size:        362     271
 
BsrFI r'CCGG_y

Cuts at:      0      84     214     250     456     633 
   Size:         84     130      36     206     177

  Fragments arranged by size: 

                 206     177     130      84      36
 
BssHII G'CGCG_C

Cuts at:      0     127     210     633 
   Size:        127      83     423

  Fragments arranged by size: 

                 423     127      83
 
BstYI r'GATC_y

Cuts at:      0      95     294     633 
   Size:         95     199     339

  Fragments arranged by size: 

                 339     199      95
 
BtgI C'CryG_G

Cuts at:      0     185     219     317     633 
   Size:        185      34      98     316

  Fragments arranged by size: 

                 316     185      98      34
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     120     633 
   Size:        120     513
 
EaeI y'GGCC_r

Cuts at:      0      65     216     248     437     633 
   Size:         65     151      32     189     196

  Fragments arranged by size: 

                 196     189     151      65      32
 
EagI C'GGCC_G

Cuts at:      0     216     633 
   Size:        216     417
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     509     577     633 
   Size:        382     127      68      56

  Fragments arranged by size: 

                 382     127      68      56
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     321     633 
   Size:        321     312
 
EcoICRI GAG'CTC

Cuts at:      0     240     633 
   Size:        240     393
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     253     370     443     633 
   Size:        210      43     117      73     190

  Fragments arranged by size: 

                 210     190     117      73      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     157     633 
   Size:        157     476
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      54     633 
   Size:         54     579
 
HaeII r_GCGC'y

Cuts at:      0       7     633 
   Size:          7     626
 
KasI G'GCGC_C

Cuts at:      0       3     633 
   Size:          3     630
 
KpnI G_GTAC'C

Cuts at:      0     198     633 
   Size:        198     435
 
MscI TGG'CCA

Cuts at:      0      67     439     633 
   Size:         67     372     194

  Fragments arranged by size: 

                 372     194      67
 
MslI CAynn'nnrTG

Cuts at:      0     205     289     633 
   Size:        205      84     344

  Fragments arranged by size: 

                 344     205      84
 
MspA1I CmG'CkG

Cuts at:      0      57     221     345     447     633 
   Size:         57     164     124     102     186

  Fragments arranged by size: 

                 186     164     124     102      57
 
NaeI GCC'GGC

Cuts at:      0      86     216     252     633 
   Size:         86     130      36     381

  Fragments arranged by size: 

                 381     130      86      36
 
NarI GG'CG_CC

Cuts at:      0       4     633 
   Size:          4     629
 
NgoMIV G'CCGG_C

Cuts at:      0      84     214     250     633 
   Size:         84     130      36     383

  Fragments arranged by size: 

                 383     130      84      36
 
PmlI CAC'GTG

Cuts at:      0      71     633 
   Size:         71     562
 
PpuMI rG'GwC_Cy

Cuts at:      0     157     633 
   Size:        157     476
 
PspXI vC'TCGA_Gb

Cuts at:      0     497     633 
   Size:        497     136
 
PstI C_TGCA'G

Cuts at:      0     332     633 
   Size:        332     301
 
PvuII CAG'CTG

Cuts at:      0     447     633 
   Size:        447     186
 
RsrII CG'GwC_CG

Cuts at:      0     454     633 
   Size:        454     179
 
SacI G_AGCT'C

Cuts at:      0     242     633 
   Size:        242     391
 
SacII CC_GC'GG

Cuts at:      0     222     633 
   Size:        222     411
 
SanDI GG'GwC_CC

Cuts at:      0     157     633 
   Size:        157     476
 
SapI GCTCTTCn'nnn_

Cuts at:      0     577     633 
   Size:        577      56
 
SbfI CC_TGCA'GG

Cuts at:      0     332     633 
   Size:        332     301
 
SfcI C'TryA_G

Cuts at:      0     328     633 
   Size:        328     305
 
SfoI GGC'GCC

Cuts at:      0       5     633 
   Size:          5     628
 
SmlI C'TyrA_G

Cuts at:      0     497     633 
   Size:        497     136
 
StyI C'CwwG_G

Cuts at:      0     122     596     633 
   Size:        122     474      37

  Fragments arranged by size: 

                 474     122      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     457     633 
   Size:        457     176
 
XhoI C'TCGA_G

Cuts at:      0     497     633 
   Size:        497     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     523     633 
   Size:        523     110
 

 Enzymes that do cut:

   Acc65I     AcuI     AgeI     AleI    AlwNI     ApoI     AscI     AvaI
    BamHI     BanI    BanII     BbeI     BglI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII    BstYI     BtgI
    BtgZI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII     KasI     KpnI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     PmlI    PpuMI    PspXI     PstI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     SfcI     SfoI     SmlI
     StyI    TaqII     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AhdI
     AlfI     AloI     ApaI    ApaLI     AseI    AsiSI    AvrII     BaeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaBI    BsaXI
    BsiWI    BsmBI    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PsrI     PvuI     SalI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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