Restriction map for
pFN21AB4568
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MunI NciI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 416 AACGTT AA^CGTT
AcsI 778 GAATTC [GA]^AATT[CT]
ApoI 778 GAATTC [GA]^AATT[CT]
AseI 295 ATTAAT AT^TAAT
AsnI 295 ATTAAT AT^TAAT
BamHI 266 GGATCC G^GATCC
BbsI 244 GTCTTC GAAGAC(2/6)
BglII 289 AGATCT A^GATCT
BlpI 1012 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1012 GCTAAGC GC^T[ACGT]AGC
BpuAI 244 GTCTTC GAAGAC(2/6)
BsmAI 771 GTCTC GTCTC(1/5)
BstEII 645 GGTAACC G^GT[ACGT]ACC
BstPI 645 GGTAACC G^GT[ACGT]ACC
BstXI 974 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1012 GCTAAGC GC^T[ACGT]AGC
DraII 50 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 7 CCGCGG C^C[GA][CT]GG
EcoO109I 50 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 645 GGTAACC G^GT[ACGT]ACC
EcoRI 778 GAATTC G^AATTC
EcoT14I 1040 CCAAGG C^C[AT][AT]GG
KspI 7 CCGCGG CCGC^GG
MaeII 417 ACGT A^CGT
NdeI 797 CATATG CA^TATG
NspI 929 GCATGC [GA]CATG^[CT]
PshBI 295 ATTAAT AT^TAAT
Psp1406I 416 AACGTT AA^CGTT
SacII 7 CCGCGG CCGC^GG
SfiI 199 GGCCGTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 929 GCATGC GCATG^C
StyI 1040 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 492 GTAC GT^AC
669 GTAC
AspHI 153 GAGCTC G[AT]GC[AT]^C
339 GAGCTC
BanII 153 GAGCTC G[GA]GC[CT]^C
339 GAGCTC
BbeI 185 GGCGCC GGCGC^C
461 GGCGCC
BmyI 153 GAGCTC G[AGT]GC[ACT]^C
339 GAGCTC
BsgI 193 CTGCAC GTGCAG(16/14)
952 CTGCAC
BsiHKAI 153 GAGCTC G[AT]GC[AT]^C
339 GAGCTC
BsiYI 270 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
466 CCAATTACAGG
BslI 270 CCAGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
466 CCAATTACAGG
Bsp1286I 153 GAGCTC G[AGT]GC[ACT]^C
339 GAGCTC
BsrFI 188 GCCGGC [GA]^CCGG[CT]
238 GCCGGC
BssHII 108 GCGCGC G^CGCGC
110 GCGCGC
BstYI 266 GGATCC [GA]^GATC[CT]
289 AGATCT
Cfr10I 188 GCCGGC [GA]^CCGG[CT]
238 GCCGGC
HapII 189 CCGG C^CGG
239 CCGG
HgaI 39 GACGC GACGC(5/10)
242 GCGTC
HpaII 189 CCGG C^CGG
239 CCGG
HphI 429 GGTGA GGTGA(8/7)
600 GGTGA
KasI 185 GGCGCC G^GCGCC
461 GGCGCC
MflI 266 GGATCC [GA]^GATC[CT]
289 AGATCT
MspI 189 CCGG C^CGG
239 CCGG
NaeI 188 GCCGGC GCC^GGC
238 GCCGGC
NarI 185 GGCGCC GG^CGCC
461 GGCGCC
NgoMIV 188 GCCGGC G^CCGGC
238 GCCGGC
PleI 347 GAGTC GAGTC(4/5)
769 GAGTC
PvuII 547 CAGCTG CAG^CTG
1080 CAGCTG
RsaI 492 GTAC GT^AC
669 GTAC
SacI 153 GAGCTC GAGCT^C
339 GAGCTC
SfaNI 577 GATGC GCATC(5/9)
753 GCATC
SfoI 185 GGCGCC GGC^GCC
461 GGCGCC
TfiI 397 GATTC G^A[AT]TC
802 GAATC
Tsp509I 468 AATT ^AATT
779 AATT
XhoII 266 GGATCC [GA]^GATC[CT]
289 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BalI BanI BbvI BglI BpmI
BsaHI BsaJI BseRI BsiEI BsmFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HinfI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp45I TspRI