Restriction map for pFN21AB4568
 (Linear) (Six-base) MAPSORT of: KIBB4568.seq  Check: 5254  from: 1  to: 1110



KIBB4568.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AclI AA'CG_TT

Cuts at:      0     417    1110 
   Size:        417     693
 
AleI CACnn'nnGTG

Cuts at:      0     517    1110 
   Size:        517     593
 
ApoI r'AATT_y

Cuts at:      0     778    1110 
   Size:        778     332
 
AseI AT'TA_AT

Cuts at:      0     296    1110 
   Size:        296     814
 
BamHI G'GATC_C

Cuts at:      0     266    1110 
   Size:        266     844
 
BanI G'GyrC_C

Cuts at:      0     185     461     475    1110 
   Size:        185     276      14     635

  Fragments arranged by size: 

                 635     276     185      14
 
BanII G_rGCy'C

Cuts at:      0     157     343    1110 
   Size:        157     186     767

  Fragments arranged by size: 

                 767     186     157
 
BbeI G_GCGC'C

Cuts at:      0     189     465    1110 
   Size:        189     276     645

  Fragments arranged by size: 

                 645     276     189
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     237    1110 
   Size:        237     873
 
BglI GCCn_nnn'nGGC

Cuts at:      0      31     206     226    1110 
   Size:         31     175      20     884

  Fragments arranged by size: 

                 884     175      31      20
 
BglII A'GATC_T

Cuts at:      0     289    1110 
   Size:        289     821
 
BlpI GC'TnA_GC

Cuts at:      0    1013    1110 
   Size:       1013      97
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     651    1110 
   Size:        651     459
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     365     616     758     964    1110 
   Size:        347      18     251     142     206     146

  Fragments arranged by size: 

                 347     251     206     146     142      18
 
BsaHI Gr'CG_yC

Cuts at:      0     186     242     462    1110 
   Size:        186      56     220     648

  Fragments arranged by size: 

                 648     220     186      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     295    1110 
   Size:        265      30     815

  Fragments arranged by size: 

                 815     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     274     289     292    1110 
   Size:        160     114      15       3     818

  Fragments arranged by size: 

                 818     160     114      15       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     937    1110 
   Size:        178     759     173

  Fragments arranged by size: 

                 759     178     173
 
BsiEI CG_ry'CG

Cuts at:      0     138     177     201    1110 
   Size:        138      39      24     909

  Fragments arranged by size: 

                 909     138      39      24
 
BsiHKAI G_wGCw'C

Cuts at:      0     157     343    1110 
   Size:        157     186     767

  Fragments arranged by size: 

                 767     186     157
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     343    1110 
   Size:        157     186     767

  Fragments arranged by size: 

                 767     186     157
 
BsrFI r'CCGG_y

Cuts at:      0     188     238    1110 
   Size:        188      50     872

  Fragments arranged by size: 

                 872     188      50
 
BssHII G'CGCG_C

Cuts at:      0     108     110    1110 
   Size:        108       2    1000

  Fragments arranged by size: 

                1000     108       2
 
BstEII G'GTnAC_C

Cuts at:      0     645    1110 
   Size:        645     465
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     981    1110 
   Size:        981     129
 
BstYI r'GATC_y

Cuts at:      0     266     289    1110 
   Size:        266      23     821

  Fragments arranged by size: 

                 821     266      23
 
BtgI C'CryG_G

Cuts at:      0       7    1110 
   Size:          7    1103
 
EaeI y'GGCC_r

Cuts at:      0      20      68     135     174     198     207     959    1110 
   Size:         20      48      67      39      24       9     752     151

  Fragments arranged by size: 

                 752     151      67      48      39      24      20       9
            
 
EagI C'GGCC_G

Cuts at:      0     135     174     198    1110 
   Size:        135      39      24     912

  Fragments arranged by size: 

                 912     135      39      24
 
EcoICRI GAG'CTC

Cuts at:      0     155     341    1110 
   Size:        155     186     769

  Fragments arranged by size: 

                 769     186     155
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     365     616     758     964    1110 
   Size:        347      18     251     142     206     146

  Fragments arranged by size: 

                 347     251     206     146     142      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51    1110 
   Size:         51    1059
 
EcoRI G'AATT_C

Cuts at:      0     778    1110 
   Size:        778     332
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     462    1110 
   Size:        462     648
 
HaeII r_GCGC'y

Cuts at:      0      64     189     465    1110 
   Size:         64     125     276     645

  Fragments arranged by size: 

                 645     276     125      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     991    1023    1110 
   Size:        991      32      87

  Fragments arranged by size: 

                 991      87      32
 
KasI G'GCGC_C

Cuts at:      0     185     461    1110 
   Size:        185     276     649

  Fragments arranged by size: 

                 649     276     185
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     892    1110 
   Size:        293     599     218

  Fragments arranged by size: 

                 599     293     218
 
MscI TGG'CCA

Cuts at:      0      70     209     961    1110 
   Size:         70     139     752     149

  Fragments arranged by size: 

                 752     149     139      70
 
MslI CAynn'nnrTG

Cuts at:      0     517     727     758    1110 
   Size:        517     210      31     352

  Fragments arranged by size: 

                 517     352     210      31
 
MspA1I CmG'CkG

Cuts at:      0       9     549    1082    1110 
   Size:          9     540     533      28

  Fragments arranged by size: 

                 540     533      28       9
 
NaeI GCC'GGC

Cuts at:      0     190     240    1110 
   Size:        190      50     870

  Fragments arranged by size: 

                 870     190      50
 
NarI GG'CG_CC

Cuts at:      0     186     462    1110 
   Size:        186     276     648

  Fragments arranged by size: 

                 648     276     186
 
NdeI CA'TA_TG

Cuts at:      0     798    1110 
   Size:        798     312
 
NgoMIV G'CCGG_C

Cuts at:      0     188     238    1110 
   Size:        188      50     872

  Fragments arranged by size: 

                 872     188      50
 
NspI r_CATG'y

Cuts at:      0     933    1110 
   Size:        933     177
 
PfoI T'CCnGG_A

Cuts at:      0     269     941    1110 
   Size:        269     672     169

  Fragments arranged by size: 

                 672     269     169
 
PvuII CAG'CTG

Cuts at:      0     549    1082    1110 
   Size:        549     533      28

  Fragments arranged by size: 

                 549     533      28
 
SacI G_AGCT'C

Cuts at:      0     157     343    1110 
   Size:        157     186     767

  Fragments arranged by size: 

                 767     186     157
 
SacII CC_GC'GG

Cuts at:      0      10    1110 
   Size:         10    1100
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     206    1110 
   Size:        206     904
 
SfoI GGC'GCC

Cuts at:      0     187     463    1110 
   Size:        187     276     647

  Fragments arranged by size: 

                 647     276     187
 
SphI G_CATG'C

Cuts at:      0     933    1110 
   Size:        933     177
 
StyI C'CwwG_G

Cuts at:      0    1040    1110 
   Size:       1040      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     493    1110 
   Size:        493     617
 
TatI w'GTAC_w

Cuts at:      0     668    1110 
   Size:        668     442
 

 Enzymes that do cut:

     AclI     AleI     ApoI     AseI    BamHI     BanI    BanII     BbeI
     BbsI     BglI    BglII     BlpI     BmrI     BpmI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrFI   BssHII   BstEII
    BstXI    BstYI     BtgI     EaeI     EagI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NspI     PfoI    PvuII
     SacI    SacII     SfiI     SfoI     SphI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BbvCI     BcgI    BciVI     BclI
    BfrBI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NcoI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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