(Circular) (Six-base) MAPSORT of: pFN21AB3573.seq Check: 822 from: 1 to: 6313 pFN21AB3573 6313 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3315 3333 3656 1937 Size: 1378 18 323 4594 Fragments arranged by size: 4594 1378 323 18 Acc65I G'GTAC_C Cuts at: 2225 2482 3057 3634 2225 Size: 257 575 577 4904 Fragments arranged by size: 4904 577 575 257 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2215 2472 2545 3373 4376 5760 6028 Size: 449 134 257 73 828 1003 1384 268 Cuts at: 6028 1632 Size: 1917 Fragments arranged by size: 1917 1384 1003 828 449 268 257 134 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 3348 5212 6189 1203 Size: 2145 1864 977 1327 Fragments arranged by size: 2145 1864 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2330 1184 Size: 1146 5167 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2328 2360 2328 Size: 32 6281 AlwNI CAG_nnn'CTG Cuts at: 2384 2655 2866 4056 5628 2384 Size: 271 211 1190 1572 3069 Fragments arranged by size: 3069 1572 1190 271 211 ApaI G_GGCC'C Cuts at: 2286 2286 Size: 6313 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 2425 3622 3848 4000 6109 1493 Size: 932 1197 226 152 2109 1697 Fragments arranged by size: 2109 1697 1197 932 226 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 AvrII C'CTAG_G Cuts at: 2911 3091 2911 Size: 180 6133 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3041 3074 3041 Size: 33 6280 BamHI G'GATC_C Cuts at: 3541 3643 4010 3541 Size: 102 367 5844 Fragments arranged by size: 5844 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2225 2482 3057 3634 Size: 325 217 627 333 105 257 575 577 Cuts at: 3634 5089 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 577 575 333 325 257 217 105 BanII G_rGCy'C Cuts at: 1391 2286 2614 3155 3413 3632 1391 Size: 895 328 541 258 219 4072 Fragments arranged by size: 4072 895 541 328 258 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3467 3501 4504 4538 1903 Size: 34 1530 34 1003 34 3678 Fragments arranged by size: 3678 1530 1003 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2843 4205 5415 2843 Size: 1362 1210 3741 Fragments arranged by size: 3741 1362 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BfrBI ATG'CAT Cuts at: 3472 3472 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3140 4924 136 Size: 107 122 71 631 605 1468 1784 1525 Fragments arranged by size: 1784 1525 1468 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 2784 3730 2784 Size: 946 5367 Bme1580I G_kGCm'C Cuts at: 1790 2125 2286 2309 4374 5530 1790 Size: 335 161 23 2065 1156 2573 Fragments arranged by size: 2573 2065 1156 335 161 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3001 4044 5002 Size: 416 53 83 399 24 1584 1043 958 Cuts at: 5002 442 Size: 1753 Fragments arranged by size: 1753 1584 1043 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3586 3586 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3060 3581 4973 1239 Size: 1821 521 1392 2579 Fragments arranged by size: 2579 1821 1392 521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2573 2660 2696 4438 5303 5601 2573 Size: 87 36 1742 865 298 3285 Fragments arranged by size: 3285 1742 865 298 87 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4976 882 Size: 617 3477 2219 Fragments arranged by size: 3477 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 4120 4502 5137 Size: 53 83 186 936 2071 516 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 2071 1451 936 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4746 5144 5418 5565 5976 4746 Size: 398 274 147 411 5083 Fragments arranged by size: 5083 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 1178 Size: 30 165 30 774 30 5284 Fragments arranged by size: 5284 774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3502 1186 Size: 2316 3997 BseYI C'CCAG_C Cuts at: 2625 2834 5516 6012 2625 Size: 209 2682 496 2926 Fragments arranged by size: 2926 2682 496 209 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5740 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2790 4374 4459 5530 2790 Size: 1584 85 1071 3573 Fragments arranged by size: 3573 1584 1071 85 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2125 2286 2309 2614 2790 3155 3413 Size: 399 335 161 23 305 176 365 258 Cuts at: 3413 3632 4374 4459 5530 1391 Size: 219 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 365 335 305 258 219 176 161 85 23 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3668 844 Size: 2824 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2690 3510 4200 1394 Size: 429 867 820 690 3507 Fragments arranged by size: 3507 867 820 690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 2898 3042 4808 4982 65 Size: 1183 934 716 144 1766 174 1396 Fragments arranged by size: 1766 1396 1183 934 716 174 144 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3049 96 Size: 2953 3360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 2120 3175 794 Size: 1326 1055 3932 Fragments arranged by size: 3932 1326 1055 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstYI r'GATC_y Cuts at: 1972 3541 3643 4010 4395 4412 5864 5950 5962 Size: 1569 102 367 385 17 1452 86 12 Cuts at: 5962 6308 1972 Size: 346 1977 Fragments arranged by size: 1977 1569 1452 385 367 346 102 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3405 3405 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 4339 Fragments arranged by size: 4339 939 552 483 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 2109 2260 2814 3465 3829 4623 4643 2109 Size: 151 554 651 364 794 20 3779 Fragments arranged by size: 3779 794 651 554 364 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DraIII CAC_nnn'GTG Cuts at: 2255 2255 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2490 3382 3760 Size: 54 1425 50 275 18 660 892 378 Cuts at: 3760 4649 6278 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 892 889 660 378 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4243 1353 Size: 2890 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2939 4900 5274 5420 387 Size: 1064 138 1350 1961 374 146 1280 Fragments arranged by size: 1961 1350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2215 2472 2545 3060 3373 3581 Size: 393 449 134 257 73 515 313 208 Cuts at: 3581 4376 4973 5760 6028 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 515 449 393 313 268 257 208 134 73 EcoNI CCTnn'n_nnAGG Cuts at: 2570 2570 Size: 6313 EcoO109I rG'GnC_Cy Cuts at: 2282 2915 3290 2282 Size: 633 375 5305 Fragments arranged by size: 5305 633 375 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 5460 1678 Size: 3782 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2457 2489 4954 4986 5028 Size: 32 135 32 885 32 2465 32 42 Cuts at: 5028 5060 1373 Size: 32 2626 Fragments arranged by size: 2626 2465 885 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3316 3657 3903 669 Size: 988 281 1378 341 246 3079 Fragments arranged by size: 3079 1378 988 341 281 246 HindIII A'AGCT_T Cuts at: 748 2143 748 Size: 1395 4918 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KpnI G_GTAC'C Cuts at: 2229 2486 3061 3638 2229 Size: 257 575 577 4904 Fragments arranged by size: 4904 577 575 257 MfeI C'AATT_G Cuts at: 3295 3912 3295 Size: 617 5696 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 3384 10 Size: 54 1425 343 660 892 2939 Fragments arranged by size: 2939 1425 892 660 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2943 3477 4271 4630 4789 518 Size: 648 1777 534 794 359 159 2042 Fragments arranged by size: 2042 1777 794 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2496 2816 3729 4406 5554 5799 1606 Size: 890 320 913 677 1148 245 2120 Fragments arranged by size: 2120 1148 913 890 677 320 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 4339 Fragments arranged by size: 4339 939 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NsiI A_TGCA'T Cuts at: 3474 3474 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3671 5216 1132 Size: 97 2442 1545 2229 Fragments arranged by size: 2442 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 2655 2866 3162 3421 1108 Size: 1547 211 296 259 4000 Fragments arranged by size: 4000 1547 296 259 211 PfoI T'CCnGG_A Cuts at: 2769 3558 2769 Size: 789 5524 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2915 3290 2915 Size: 375 5938 PshAI GACnn'nnGTC Cuts at: 1613 2913 1613 Size: 1300 5013 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 2282 2282 Size: 6313 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3221 3253 3221 Size: 32 6281 PstI C_TGCA'G Cuts at: 830 2331 2619 3665 830 Size: 1501 288 1046 3478 Fragments arranged by size: 3478 1501 1046 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2496 2816 2496 Size: 320 5993 SalI G'TCGA_C Cuts at: 1936 3314 3655 1936 Size: 1378 341 4594 Fragments arranged by size: 4594 1378 341 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 2935 4561 6273 2935 Size: 1626 1712 2975 Fragments arranged by size: 2975 1712 1626 SfcI C'TryA_G Cuts at: 826 1045 2152 2327 2615 3661 4796 5477 5668 Size: 219 1107 175 288 1046 1135 681 191 Cuts at: 5668 826 Size: 1471 Fragments arranged by size: 1471 1135 1107 1046 681 288 219 191 175 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2588 2675 2711 4417 5318 5580 Size: 197 926 645 87 36 1706 901 262 Cuts at: 5580 820 Size: 1553 Fragments arranged by size: 1706 1553 926 901 645 262 197 87 36 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 2313 3143 1517 Size: 796 830 4687 Fragments arranged by size: 4687 830 796 StyI C'CwwG_G Cuts at: 513 1065 2004 2221 2487 2911 3091 3342 3379 Size: 552 939 217 266 424 180 251 37 Cuts at: 3379 3752 513 Size: 373 3074 Fragments arranged by size: 3074 939 552 424 373 266 251 217 180 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2680 3272 3289 4340 4357 4510 4695 Size: 1255 1100 592 17 1051 17 153 185 Cuts at: 4695 5140 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1100 1051 592 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2246 2933 3049 Size: 274 80 33 51 1443 269 687 116 Cuts at: 3049 3207 4559 6271 96 Size: 158 1352 1712 138 Fragments arranged by size: 1712 1443 1352 687 274 269 158 138 116 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6273 6305 6273 Size: 32 6281 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 4442 4442 Size: 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI