Restriction map for
pFN21AB3382
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BamHI BbeI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstYI BstZ17I CelII
ClaI CpoI DpnII DraI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MboI MfeI
MflI MluI MseI MunI MvnI
NarI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfcI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 195 TCCGGA T^CCGGA
AcsI 870 GAATTC [GA]^AATT[CT]
AcyI 1150 GACGCC G[GA]^CG[CT]C
AflIII 779 ACGTGT A^C[GA][CT]GT
AhdI 665 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 533 CCTCAGG CC^T[ACGT]AGG
ApoI 870 GAATTC [GA]^AATT[CT]
AspEI 665 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 418 GTCTTC GAAGAC(2/6)
BciVI 187 GTATCC GTATCC(6/5)
BpuAI 418 GTCTTC GAAGAC(2/6)
BsaHI 1150 GACGCC G[GA]^CG[CT]C
BsaI 72 GGTCTC GGTCTC(1/5)
BsaWI 195 TCCGGA [AT]^CCGG[AT]
BseAI 195 TCCGGA T^CCGGA
BspEI 195 TCCGGA T^CCGGA
BspMI 498 GCAGGT ACCTGC(4/8)
BsrDI 278 GCAATG GCAATG(2/0)
BstEII 571 GGTGACC G^GT[ACGT]ACC
BstPI 571 GGTGACC G^GT[ACGT]ACC
BstXI 807 CCATCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 533 CCTCAGG CC^T[ACGT]AGG
CfoI 12 GCGC GCG^C
DsaI 639 CCGTGG C^C[GA][CT]GG
Eam1105I 665 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 533 CCTCAGG CC^T[ACGT]AGG
EcoNI 981 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 571 GGTGACC G^GT[ACGT]ACC
EcoRI 870 GAATTC G^AATTC
HgaI 1150 GACGC GACGC(5/10)
HhaI 12 GCGC GCG^C
Hin1I 1150 GACGCC G[GA]^CG[CT]C
HinP1I 12 GCGC G^CGC
MroI 195 TCCGGA T^CCGGA
MslI 306 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 227 GCATGC [GA]CATG^[CT]
PflMI 878 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 665 GACTC GAGTC(4/5)
SphI 227 GCATGC GCATG^C
StuI 654 AGGCCT AGG^CCT
TaqI 659 TCGA T^CGA
Tsp509I 871 AATT ^AATT
TspRI 311 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 659 TCGA T^CGA
Van91I 878 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BalI 255 TGGCCA TGG^CCA
903 TGGCCA
BbrPI 715 CACGTG CAC^GTG
778 CACGTG
BbvCI 214 GCTGAGG CCTCAGC(-5/-2)
994 GCTGAGG
BfaI 118 CTAG C^TAG
610 CTAG
BglI 605 GCCAGCTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
647 GCCGGCAAGGC
BsaAI 715 CACGTG [CT]AC^GT[GA]
778 CACGTG
BseRI 1041 CTCCTC GAGGAG(10/8)
997 GAGGAG
BsrFI 177 GCCGGC [GA]^CCGG[CT]
647 GCCGGC
Cfr10I 177 GCCGGC [GA]^CCGG[CT]
647 GCCGGC
EarI 480 GAAGAG CTCTTC(1/4)
1002 GAAGAG
FauI 593 GCGGG CCCGC(4/6)
1217 GCGGG
Ksp632I 480 GAAGAG CTCTTC(1/4)
1002 GAAGAG
MaeI 118 CTAG C^TAG
610 CTAG
MluNI 255 TGGCCA TGG^CCA
903 TGGCCA
MscI 255 TGGCCA TGG^CCA
903 TGGCCA
NaeI 177 GCCGGC GCC^GGC
647 GCCGGC
NgoMIV 177 GCCGGC G^CCGGC
647 GCCGGC
PmaCI 715 CACGTG CAC^GTG
778 CACGTG
PmlI 715 CACGTG CAC^GTG
778 CACGTG
PpuMI 28 AGGTCCC [GA]G^G[AT]CC[CT]
82 AGGTCCC
SapI 480 GAAGAGC GCTCTTC(1/4)
1002 GAAGAGC
SfiI 604 GGCCAGCTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
646 GGCCGGCAAGGCC
XcmI 878 CCAAGTCCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1118 CCAACTACCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII DraIII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI SfaNI StyI TfiI
TseI Tsp45I