(Circular) (Six-base) MAPSORT of: pFN21AB3382.seq Check: 9249 from: 1 to: 5968 pFN21AB3382 5968 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2494 2494 Size: 5968 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4031 5415 5683 1632 Size: 2399 1384 268 1917 Fragments arranged by size: 2399 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 2784 4867 5844 1203 Size: 1581 2083 977 1327 Fragments arranged by size: 2083 1581 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 2675 4697 2675 Size: 2022 3946 AleI CACnn'nnGTG Cuts at: 1166 2315 1166 Size: 1149 4819 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2849 2881 2849 Size: 32 5936 AlwNI CAG_nnn'CTG Cuts at: 3711 5283 3711 Size: 1572 4396 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2875 3277 3503 3655 5764 1493 Size: 1382 402 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 402 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 3293 1870 Size: 73 1350 4545 Fragments arranged by size: 4545 1350 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2360 2393 2360 Size: 33 5935 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2294 2494 2747 3119 3289 Size: 325 217 627 507 200 253 372 170 Cuts at: 3289 4744 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 507 372 325 253 217 200 170 BanII G_rGCy'C Cuts at: 1391 2327 2438 2478 3167 3287 1391 Size: 936 111 40 689 120 4072 Fragments arranged by size: 4072 936 689 120 111 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2416 928 Size: 971 517 4480 Fragments arranged by size: 4480 971 517 BbvCI CC'TCA_GC Cuts at: 2220 3000 2220 Size: 780 5188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2203 3860 5070 2203 Size: 1657 1210 3101 Fragments arranged by size: 3101 1657 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2616 2658 4579 Size: 107 122 71 631 605 944 42 1921 Cuts at: 4579 136 Size: 1525 Fragments arranged by size: 1921 1525 944 631 605 122 107 71 42 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 2299 2834 3122 3140 4029 5185 1790 Size: 509 535 288 18 889 1156 2573 Fragments arranged by size: 2573 1156 889 535 509 288 18 BmgBI CAC'GTC Cuts at: 1318 2137 1318 Size: 819 5149 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3699 4657 442 Size: 416 53 83 399 24 2282 958 1753 Fragments arranged by size: 2282 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3013 3181 3212 4628 1239 Size: 1774 168 31 1416 2579 Fragments arranged by size: 2579 1774 1416 168 31 Bpu10I CC'TnA_GC Cuts at: 2220 3000 2220 Size: 780 5188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4093 4958 5256 4093 Size: 865 298 4805 Fragments arranged by size: 4805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2083 4631 882 Size: 617 584 2548 2219 Fragments arranged by size: 2548 2219 617 584 BsaAI yAC'GTr Cuts at: 493 1204 2722 2785 3759 493 Size: 711 1518 63 974 2702 Fragments arranged by size: 2702 1518 974 711 63 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3775 4157 4792 Size: 53 83 186 936 1623 619 382 635 Cuts at: 4792 275 Size: 1451 Fragments arranged by size: 1623 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2200 4401 4799 5073 5220 5631 2200 Size: 2201 398 274 147 411 2537 Fragments arranged by size: 2537 2201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5743 Fragments arranged by size: 5743 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3017 3037 1186 Size: 1831 20 4117 Fragments arranged by size: 4117 1831 20 BseYI C'CCAG_C Cuts at: 2041 2146 2152 2225 2980 3133 5171 5667 2041 Size: 105 6 73 755 153 2038 496 2342 Fragments arranged by size: 2342 2038 755 496 153 105 73 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2522 2528 1163 Size: 573 498 288 6 4603 Fragments arranged by size: 4603 573 498 288 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4029 4114 5185 4029 Size: 85 1071 4812 Fragments arranged by size: 4812 1071 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 2327 2438 2478 2834 3122 3140 Size: 399 509 28 111 40 356 288 18 Cuts at: 3140 3167 3287 4029 4114 5185 1391 Size: 27 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 399 356 288 120 111 85 40 28 27 18 BspEI T'CCGG_A Cuts at: 2200 5631 2200 Size: 3431 2537 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2494 3323 844 Size: 1650 829 3489 Fragments arranged by size: 3489 1650 829 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 4463 4637 65 Size: 1183 1042 2173 174 1396 Fragments arranged by size: 2173 1396 1183 1042 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 2652 4612 4799 1700 Size: 110 372 470 1960 187 2869 Fragments arranged by size: 2869 1960 470 372 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 2576 1184 Size: 1392 4576 BstXI CCAn_nnnn'nTGG Cuts at: 2819 2819 Size: 5968 BstYI r'GATC_y Cuts at: 1972 3298 3665 4050 4067 5519 5605 5617 5963 Size: 1326 367 385 17 1452 86 12 346 Cuts at: 5963 1972 Size: 1977 Fragments arranged by size: 1977 1452 1326 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2539 2539 Size: 5968 BtgI C'CryG_G Cuts at: 513 1065 2644 513 Size: 552 1579 3837 Fragments arranged by size: 3837 1579 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 3484 4278 4298 3484 Size: 794 20 5154 Fragments arranged by size: 5154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2629 2664 529 Size: 35 2065 35 3833 Fragments arranged by size: 3833 2065 35 35 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DraIII CAC_nnn'GTG Cuts at: 2242 2244 2557 2242 Size: 2 313 5653 Fragments arranged by size: 5653 313 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 2416 2650 Size: 54 1425 50 275 18 430 156 234 Cuts at: 2650 2908 3415 4304 5933 8 Size: 258 507 889 1629 43 Fragments arranged by size: 1629 1425 889 507 430 275 258 234 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 3002 3898 1353 Size: 1127 522 896 3423 Fragments arranged by size: 3423 1127 896 522 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3013 3181 3212 4031 4628 5415 5683 Size: 393 1381 168 31 819 597 787 268 Cuts at: 5683 1239 Size: 1524 Fragments arranged by size: 1524 1381 819 787 597 393 268 168 31 EcoNI CCTnn'n_nnAGG Cuts at: 2990 2990 Size: 5968 EcoO109I rG'GnC_Cy Cuts at: 2034 2088 3063 2034 Size: 54 975 4939 Fragments arranged by size: 4939 975 54 EcoRI G'AATT_C Cuts at: 2875 3277 3655 2875 Size: 402 378 5188 Fragments arranged by size: 5188 402 378 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 5115 1678 Size: 3437 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2661 2693 4609 4641 4683 Size: 32 135 32 1089 32 1916 32 42 Cuts at: 4683 4715 1373 Size: 32 2626 Fragments arranged by size: 2626 1916 1089 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3558 669 Size: 988 281 1374 246 3079 Fragments arranged by size: 3079 1374 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 2829 5082 5266 1305 Size: 1146 378 2253 184 2007 Fragments arranged by size: 2253 2007 1146 378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 2910 10 Size: 54 1425 343 430 648 3068 Fragments arranged by size: 3068 1425 648 430 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 3926 4285 4444 518 Size: 648 1149 1611 359 159 2042 Fragments arranged by size: 2042 1611 1149 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2512 2570 3133 3190 3222 3384 4061 Size: 540 366 58 563 57 32 162 677 Cuts at: 4061 5209 5454 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 563 540 366 245 162 58 57 32 NaeI GCC'GGC Cuts at: 2184 2654 2184 Size: 470 5498 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5416 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NgoMIV G'CCGG_C Cuts at: 2182 2652 2182 Size: 470 5498 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2236 3326 4871 1132 Size: 97 1007 1090 1545 2229 Fragments arranged by size: 2229 1545 1090 1007 97 PasI CC'CwG_GG Cuts at: 1348 2299 2339 1348 Size: 951 40 4977 Fragments arranged by size: 4977 951 40 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 2889 1108 Size: 1781 4187 PfoI T'CCnGG_A Cuts at: 2442 2730 2442 Size: 288 5680 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PmlI CAC'GTG Cuts at: 2722 2785 2722 Size: 63 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2088 2034 Size: 54 5914 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PstI C_TGCA'G Cuts at: 830 3320 830 Size: 2490 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2146 2512 2570 3133 3190 2146 Size: 366 58 563 57 4924 Fragments arranged by size: 4924 563 366 58 57 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SapI GCTCTTCn'nnn_ Cuts at: 2480 3002 2480 Size: 522 5446 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 3316 4451 5132 5323 826 Size: 219 2271 1135 681 191 1471 Fragments arranged by size: 2271 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2616 2658 2616 Size: 42 5926 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 3295 1872 Size: 1423 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4072 4973 5235 820 Size: 197 926 2129 901 262 1553 Fragments arranged by size: 2129 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 2236 3326 1132 Size: 1104 1090 3774 Fragments arranged by size: 3774 1104 1090 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 2661 1517 Size: 1144 4824 StyI C'CwwG_G Cuts at: 513 1065 2589 2986 3087 3407 513 Size: 552 1524 397 101 320 3074 Fragments arranged by size: 3074 1524 552 397 320 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2144 2881 3995 4012 4165 4350 Size: 1255 446 118 737 1114 17 153 185 Cuts at: 4350 4795 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1114 737 446 445 185 153 118 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4214 5926 96 Size: 274 80 33 51 1443 2237 1712 138 Fragments arranged by size: 2237 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5928 5960 5928 Size: 32 5936 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2890 3130 2890 Size: 240 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 3293 1870 Size: 1423 4545 XmnI GAAnn'nnTTC Cuts at: 4097 4097 Size: 5968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI