Restriction map for
pFN21AE0914
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaII AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SacI SalI SapI Sau96I ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tru9I
TspRI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AclI 10 AACGTT AA^CGTT
AcsI 154 AAATTC [GA]^AATT[CT]
AcyI 129 GGCGCC G[GA]^CG[CT]C
AfaI 528 GTAC GT^AC
AlwI 537 GGATC GGATC(4/5)
ApoI 154 AAATTC [GA]^AATT[CT]
Asp700I 150 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 715 GTGCTC G[AT]GC[AT]^C
AspI 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 703 CCCGAG C^[CT]CG[GA]G
BanI 129 GGCGCC G^G[CT][GA]CC
BbeI 129 GGCGCC GGCGC^C
BbsI 580 GTCTTC GAAGAC(2/6)
BfaI 188 CTAG C^TAG
BpuAI 580 GTCTTC GAAGAC(2/6)
BsaHI 129 GGCGCC G[GA]^CG[CT]C
BsaI 364 GAGACC GGTCTC(1/5)
BsgI 55 GTGCAG GTGCAG(16/14)
BsiHKAI 715 GTGCTC G[AT]GC[AT]^C
BsmBI 432 CGTCTC CGTCTC(1/5)
BsmFI 183 GTCCC GGGAC(10/14)
BsoBI 703 CCCGAG C^[CT]CG[GA]G
BspMI 530 ACCTGC ACCTGC(4/8)
BssHII 463 GCGCGC G^CGCGC
BstEII 303 GGTGACC G^GT[ACGT]ACC
BstF5I 387 CATCC GGATG(2/0)
BstPI 303 GGTGACC G^GT[ACGT]ACC
BstYI 537 GGATCT [GA]^GATC[CT]
DpnII 538 GATC ^GATC
EcoO65I 303 GGTGACC G^GT[ACGT]ACC
EcoRV 142 GATATC GAT^ATC
FokI 387 CATCC GGATG(9/13)
HgaI 506 GCGTC GACGC(5/10)
Hin1I 129 GGCGCC G[GA]^CG[CT]C
KasI 129 GGCGCC G^GCGCC
KspI 495 CCGCGG CCGC^GG
MaeI 188 CTAG C^TAG
MboI 538 GATC ^GATC
MflI 537 GGATCT [GA]^GATC[CT]
NarI 129 GGCGCC GG^CGCC
NdeII 538 GATC ^GATC
NlaIV 129 GGCGCC GG[ACGT]^[ACGT]CC
PflFI 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 10 AACGTT AA^CGTT
PvuII 292 CAGCTG CAG^CTG
RsaI 528 GTAC GT^AC
SacII 495 CCGCGG CCGC^GG
Sau3AI 538 GATC ^GATC
SbfI 531 CCTGCAGG CCTGCA^GG
SfaNI 563 GCATC GCATC(5/9)
SfoI 129 GGCGCC GGC^GCC
Sse8387I 531 CCTGCAGG CCTGCA^GG
TfiI 661 GATTC G^A[AT]TC
Tsp509I 155 AATT ^AATT
Tth111I 678 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 537 GGATCT [GA]^GATC[CT]
XmnI 150 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 464 CGCG CG^CG
496 CGCG
AlwNI 504 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
535 CAGGATCTG
BanII 202 GGGCTC G[GA]GC[CT]^C
706 GAGCCC
BsiEI 284 CGGCCG CG[GA][CT]^CG
416 CGGCCG
BsiYI 490 CCCGCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCCCTCGGTGG
BslI 490 CCCGCCCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
570 CCCCTCGGTGG
BsrBI 195 CCGCTC CCGCTC(-3/-3)
405 GAGCGG
BstUI 464 CGCG CG^CG
496 CGCG
DsaI 281 CCACGG C^C[GA][CT]GG
495 CCGCGG
EagI 284 CGGCCG C^GGCCG
416 CGGCCG
EclXI 284 CGGCCG C^GGCCG
416 CGGCCG
Eco52I 284 CGGCCG C^GGCCG
416 CGGCCG
MaeII 11 ACGT A^CGT
221 ACGT
MaeIII 229 GTCAC ^GT[ACGT]AC
304 GTGAC
MvnI 464 CGCG CG^CG
496 CGCG
NlaIII 327 CATG CATG^
721 CATG
PstI 118 CTGCAG CTGCA^G
532 CTGCAG
SfcI 118 CTGCAG C^T[GA][CT]AG
532 CTGCAG
Tsp45I 229 GTCAC ^GT[GC]AC
304 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BpmI BsaJI BseRI BsmAI Bsp1286I
BssKI BstNI Cac8I CfoI DdeI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MspA1I MspI MvaI MwoI NciI
PleI ScrFI TaqI TseI TthHB8I