(Circular) (Six-base) MAPSORT of: pFN21AE0914.seq Check: 3115 from: 1 to: 5476 pFN21AE0914 5476 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3286 278 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 2016 3603 3976 1563 Size: 453 1587 373 3063 Fragments arranged by size: 3063 1587 453 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 4923 5191 1632 Size: 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 4205 4205 Size: 5476 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 2514 2545 3219 4791 2514 Size: 31 674 1572 3199 Fragments arranged by size: 3199 1572 674 31 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2159 2785 3011 3163 5272 1493 Size: 666 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2708 2801 1870 Size: 73 765 93 4545 Fragments arranged by size: 4545 765 93 73 BamHI G'GATC_C Cuts at: 2806 3173 2806 Size: 367 5109 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 2797 4252 618 Size: 325 217 627 347 663 1455 1842 Fragments arranged by size: 1842 1455 663 627 347 325 217 BanII G_rGCy'C Cuts at: 1391 2211 2715 2795 1391 Size: 820 504 80 4072 Fragments arranged by size: 4072 820 504 80 BbeI G_GCGC'C Cuts at: 2138 2138 Size: 5476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 928 Size: 971 679 3826 Fragments arranged by size: 3826 971 679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3667 3701 1903 Size: 34 50 34 1646 34 3678 Fragments arranged by size: 3678 1646 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2893 2893 Size: 5476 Bme1580I G_kGCm'C Cuts at: 1790 3537 4693 1790 Size: 1747 1156 2573 Fragments arranged by size: 2573 1747 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2509 2626 2781 4136 1239 Size: 1082 188 117 155 1355 2579 Fragments arranged by size: 2579 1355 1082 188 155 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 4139 882 Size: 617 864 1776 2219 Fragments arranged by size: 2219 1776 864 617 BsaAI yAC'GTr Cuts at: 493 1204 3267 493 Size: 711 2063 2702 Fragments arranged by size: 2702 2063 711 BsaBI GATnn'nnATC Cuts at: 5470 5470 Size: 5476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2135 3283 3665 4300 Size: 53 83 186 936 602 1148 382 635 Cuts at: 4300 275 Size: 1451 Fragments arranged by size: 1451 1148 936 635 602 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2319 2349 1178 Size: 30 165 30 916 30 4305 Fragments arranged by size: 4305 916 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2102 2340 2668 2744 1186 Size: 913 3 238 328 76 3918 Fragments arranged by size: 3918 913 328 238 76 3 BseYI C'CCAG_C Cuts at: 2138 2320 4679 5175 2138 Size: 182 2359 496 2439 Fragments arranged by size: 2439 2359 496 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 4558 Fragments arranged by size: 4558 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 2424 2926 3687 4715 1540 Size: 275 186 291 132 502 761 1028 2301 Fragments arranged by size: 2301 1028 761 502 291 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2724 3537 3622 4693 2724 Size: 813 85 1071 3507 Fragments arranged by size: 3507 1071 813 85 BsmI GAATG_Cn' Cuts at: 2987 3080 2987 Size: 93 5383 BsmBI CGTCTCn'nnnn_ Cuts at: 2443 2443 Size: 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2715 2724 2795 3537 3622 4693 Size: 399 421 504 9 71 742 85 1071 Cuts at: 4693 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 504 421 399 85 71 9 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2544 2831 844 Size: 1700 287 3489 Fragments arranged by size: 3489 1700 287 BsrBI CCG'CTC Cuts at: 1394 1823 2202 2412 3363 1394 Size: 429 379 210 951 3507 Fragments arranged by size: 3507 951 429 379 210 BsrDI GCAATG_nn' Cuts at: 65 1248 3971 4145 65 Size: 1183 2723 174 1396 Fragments arranged by size: 2723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4120 4307 1700 Size: 110 2310 187 2869 Fragments arranged by size: 2869 2310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 2468 1925 Size: 543 4933 BssSI C'ACGA_G Cuts at: 3536 4548 3536 Size: 1012 4464 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 2308 1184 Size: 1124 4352 BstYI r'GATC_y Cuts at: 1972 2542 2806 3173 3558 3575 5027 5113 5125 Size: 570 264 367 385 17 1452 86 12 Cuts at: 5125 5471 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 570 385 367 346 264 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2286 2500 513 Size: 552 1221 214 3489 Fragments arranged by size: 3489 1221 552 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2421 2503 Size: 54 1425 50 275 18 459 132 82 Cuts at: 2503 2923 3812 5441 8 Size: 420 889 1629 43 Fragments arranged by size: 1629 1425 889 459 420 275 132 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2289 2421 2923 1537 Size: 275 477 132 502 4090 Fragments arranged by size: 4090 502 477 275 132 EarI CTCTTCn'nnn_ Cuts at: 1353 2767 3406 1353 Size: 1414 639 3423 Fragments arranged by size: 3423 1414 639 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2509 2626 2781 3539 4136 4923 Size: 393 689 188 117 155 758 597 787 Cuts at: 4923 5191 1239 Size: 268 1524 Fragments arranged by size: 1524 787 758 689 597 393 268 188 155 117 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 EcoRV GAT'ATC Cuts at: 2149 2149 Size: 5476 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2138 2553 2649 4623 1678 Size: 460 415 96 1974 2531 Fragments arranged by size: 2531 1974 460 415 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2597 2629 4117 Size: 32 135 32 467 32 526 32 1488 Cuts at: 4117 4149 4191 4223 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 526 467 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2134 2134 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3654 Fragments arranged by size: 3654 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2481 2502 2892 3569 4717 4962 1606 Size: 693 182 21 390 677 1148 245 2120 Fragments arranged by size: 2120 1148 693 677 390 245 182 21 NarI GG'CG_CC Cuts at: 2135 2135 Size: 5476 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4924 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5476 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PstI C_TGCA'G Cuts at: 830 2127 2541 2828 830 Size: 1297 414 287 3478 Fragments arranged by size: 3478 1297 414 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5476 SacII CC_GC'GG Cuts at: 2503 2503 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2541 2828 2541 Size: 287 5189 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2123 2537 2824 3959 4640 4831 826 Size: 219 1078 414 287 1135 681 191 1471 Fragments arranged by size: 1471 1135 1078 681 414 287 219 191 SfoI GGC'GCC Cuts at: 2136 2136 Size: 5476 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2803 1872 Size: 931 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2915 513 Size: 552 1850 3074 Fragments arranged by size: 3074 1850 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 3503 3520 3673 3858 4303 325 Size: 1255 747 1176 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1176 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 2390 2422 5436 5468 2319 Size: 32 39 32 3014 32 2327 Fragments arranged by size: 3014 2327 39 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 1316 Size: 1370 4106 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2801 1870 Size: 931 4545 XmnI GAAnn'nnTTC Cuts at: 2159 3605 2159 Size: 1446 4030 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3284 276 Size: 53 83 186 936 1750 2468 Fragments arranged by size: 2468 1750 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI