Restriction map for
pFN21AE0834
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KspI
MaeII MamI MfeI MluI MroI
MseI MspI MunI MvnI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 625 GGTACC G^GTACC
AocI 543 CCTGAGG CC^T[ACGT]AGG
Asp700I 17 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 625 GGTACC G^GTACC
AvaII 307 GGTCC G^G[AT]CC
BamHI 548 GGATCC G^GATCC
BanI 625 GGTACC G^G[CT][GA]CC
BanII 99 GAGCCC G[GA]GC[CT]^C
BbsI 490 GTCTTC GAAGAC(2/6)
BbvCI 54 CCTCAGC CCTCAGC(-5/-2)
BfaI 298 CTAG C^TAG
BmyI 99 GAGCCC G[AGT]GC[ACT]^C
BpmI 476 CTGGAG CTGGAG(16/14)
BpuAI 490 GTCTTC GAAGAC(2/6)
BseRI 87 CTCCTC GAGGAG(10/8)
BsmAI 362 GTCTC GTCTC(1/5)
Bsp1286I 99 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 645 TGTACA T^GTACA
BspHI 326 TCATGA T^CATGA
BspMI 79 ACCTGC ACCTGC(4/8)
BsrBI 552 CCGCTC CCGCTC(-3/-3)
BsrGI 645 TGTACA T^GTACA
BsrI 653 CCAGT ACTGG(1/-1)
Bsu36I 543 CCTGAGG CC^T[ACGT]AGG
DraII 306 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 218 CCATGG C^C[GA][CT]GG
Eco81I 543 CCTGAGG CC^T[ACGT]AGG
EcoO109I 306 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 353 ATGCAT ATGCA^T
FauI 26 CCCGC CCCGC(4/6)
HphI 597 TCACC GGTGA(8/7)
KpnI 625 GGTACC GGTAC^C
MaeI 298 CTAG C^TAG
MspA1I 605 CAGCTG C[AC]G^C[GT]G
NcoI 218 CCATGG C^CATGG
NsiI 353 ATGCAT ATGCA^T
PflMI 294 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 306 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 605 CAGCTG CAG^CTG
RcaI 326 TCATGA T^CATGA
SapI 590 GCTCTTC GCTCTTC(1/4)
SfcI 735 CTACAG C^T[GA][CT]AG
SmlI 741 CTTGAG C^T[CT][GA]AG
SspBI 645 TGTACA T^GTACA
Tsp45I 781 GTCAC ^GT[GC]AC
Tsp509I 18 AATT ^AATT
Van91I 294 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 17 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 27 CCGC CCGC(-3/-1)
552 CCGC
BalI 239 TGGCCA TGG^CCA
536 TGGCCA
BbvI 165 GCAGC GCAGC(8/12)
193 GCAGC
BssKI 443 CCAGG ^CC[ACGT]GG
669 CCTGG
BstNI 443 CCAGG CC^[AT]GG
669 CCTGG
BstYI 548 GGATCC [GA]^GATC[CT]
672 GGATCT
Cfr13I 307 GGTCC G^G[ACGT]CC
376 GGCCC
DpnII 549 GATC ^GATC
673 GATC
EaeI 239 TGGCCA [CT]^GGCC[GA]
536 TGGCCA
EarI 591 CTCTTC CTCTTC(1/4)
129 GAAGAG
EcoRII 443 CCAGG ^CC[AT]GG
669 CCTGG
EcoT14I 150 CCTTGG C^C[AT][AT]GG
218 CCATGG
Fnu4HI 165 GCAGC GC^[ACGT]GC
193 GCAGC
ItaI 165 GCAGC GC^[ACGT]GC
193 GCAGC
Ksp632I 591 CTCTTC CTCTTC(1/4)
129 GAAGAG
MaeIII 248 GTAAC ^GT[ACGT]AC
781 GTCAC
MboI 549 GATC ^GATC
673 GATC
MflI 548 GGATCC [GA]^GATC[CT]
672 GGATCT
MluNI 239 TGGCCA TGG^CCA
536 TGGCCA
MscI 239 TGGCCA TGG^CCA
536 TGGCCA
MvaI 443 CCAGG CC^[AT]GG
669 CCTGG
NdeII 549 GATC ^GATC
673 GATC
Sau3AI 549 GATC ^GATC
673 GATC
Sau96I 307 GGTCC G^G[ACGT]CC
376 GGCCC
ScrFI 443 CCAGG CC^[ACGT]GG
669 CCTGG
StyI 150 CCTTGG C^C[AT][AT]GG
218 CCATGG
TfiI 22 GATTC G^A[AT]TC
711 GATTC
TseI 165 GCAGC G^C[AT]GC
193 GCAGC
XhoII 548 GGATCC [GA]^GATC[CT]
672 GGATCT
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI AlwI BsaJI BsiYI
BslI BsmFI BstF5I DdeI FokI
HaeIII HinfI MboII MnlI MslI
NlaIII NlaIV PleI RsaI SfaNI
TspRI