Restriction map for
pFN21AE0834
(Linear) (Six-base) MAPSORT of: KIEE0834.seq Check: 9759 from: 1 to: 807
KIEE0834.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 88 807
Size: 88 719
Acc65I G'GTAC_C
Cuts at: 0 625 807
Size: 625 182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 807
Size: 477 330
AleI CACnn'nnGTG
Cuts at: 0 706 807
Size: 706 101
BamHI G'GATC_C
Cuts at: 0 548 807
Size: 548 259
BanI G'GyrC_C
Cuts at: 0 625 807
Size: 625 182
BanII G_rGCy'C
Cuts at: 0 103 807
Size: 103 704
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 807
Size: 483 324
BbvCI CC'TCA_GC
Cuts at: 0 55 807
Size: 55 752
BfrBI ATG'CAT
Cuts at: 0 355 807
Size: 355 452
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 807
Size: 647 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 807
Size: 497 310
Bpu10I CC'TnA_GC
Cuts at: 0 55 807
Size: 55 752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 807
Size: 762 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 807
Size: 78 729
Bsp1286I G_dGCh'C
Cuts at: 0 103 807
Size: 103 704
BspHI T'CATG_A
Cuts at: 0 326 807
Size: 326 481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 807
Size: 88 719
BsrBI CCG'CTC
Cuts at: 0 554 807
Size: 554 253
BsrGI T'GTAC_A
Cuts at: 0 645 807
Size: 645 162
BstYI r'GATC_y
Cuts at: 0 548 672 807
Size: 548 124 135
Fragments arranged by size:
548 135 124
Bsu36I CC'TnA_GG
Cuts at: 0 544 807
Size: 544 263
BtgI C'CryG_G
Cuts at: 0 218 807
Size: 218 589
BtsI GCAGTG_nn'
Cuts at: 0 175 713 807
Size: 175 538 94
Fragments arranged by size:
538 175 94
EaeI y'GGCC_r
Cuts at: 0 239 536 807
Size: 239 297 271
Fragments arranged by size:
297 271 239
EarI CTCTTCn'nnn_
Cuts at: 0 124 597 807
Size: 124 473 210
Fragments arranged by size:
473 210 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 497 807
Size: 477 20 310
Fragments arranged by size:
477 310 20
EcoO109I rG'GnC_Cy
Cuts at: 0 307 807
Size: 307 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 768 800 807
Size: 768 32 7
Fragments arranged by size:
768 32 7
KpnI G_GTAC'C
Cuts at: 0 629 807
Size: 629 178
MscI TGG'CCA
Cuts at: 0 241 538 807
Size: 241 297 269
Fragments arranged by size:
297 269 241
MslI CAynn'nnrTG
Cuts at: 0 40 532 653 706 807
Size: 40 492 121 53 101
Fragments arranged by size:
492 121 101 53 40
MspA1I CmG'CkG
Cuts at: 0 607 807
Size: 607 200
NcoI C'CATG_G
Cuts at: 0 218 807
Size: 218 589
NsiI A_TGCA'T
Cuts at: 0 357 807
Size: 357 450
PflMI CCAn_nnn'nTGG
Cuts at: 0 300 807
Size: 300 507
PpuMI rG'GwC_Cy
Cuts at: 0 307 807
Size: 307 500
PvuII CAG'CTG
Cuts at: 0 607 807
Size: 607 200
SanDI GG'GwC_CC
Cuts at: 0 307 807
Size: 307 500
SapI GCTCTTCn'nnn_
Cuts at: 0 597 807
Size: 597 210
SfcI C'TryA_G
Cuts at: 0 735 807
Size: 735 72
SmlI C'TyrA_G
Cuts at: 0 741 807
Size: 741 66
StyI C'CwwG_G
Cuts at: 0 150 218 807
Size: 150 68 589
Fragments arranged by size:
589 150 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 807
Size: 54 753
TatI w'GTAC_w
Cuts at: 0 44 645 807
Size: 44 601 162
Fragments arranged by size:
601 162 44
XmnI GAAnn'nnTTC
Cuts at: 0 21 807
Size: 21 786
Enzymes that do cut:
AarI Acc65I AcuI AleI BamHI BanI BanII BbsI
BbvCI BfrBI BmrI BpmI Bpu10I BpuEI BseRI Bsp1286I
BspHI BspMI BsrBI BsrGI BstYI Bsu36I BtgI BtsI
EaeI EarI Eco57MI EcoO109I Hin4I KpnI MscI MslI
MspA1I NcoI NsiI PflMI PpuMI PvuII SanDI SapI
SfcI SmlI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI