(Circular) (Six-base) MAPSORT of: pFN21AB0430.seq Check: 8593 from: 1 to: 7261 pFN21AB0430 7261 bp With 182 enzymes: SgfI * April 18, 2011 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2622 2622 Size: 7261 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5071 278 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3070 4604 1937 Size: 1133 1534 4594 Fragments arranged by size: 4594 1534 1133 Acc65I G'GTAC_C Cuts at: 4222 4582 4222 Size: 360 6901 AclI AA'CG_TT Cuts at: 1563 5388 5761 1563 Size: 3825 373 3063 Fragments arranged by size: 3825 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2956 2980 3162 5324 6708 6976 1632 Size: 1324 24 182 2162 1384 268 1917 Fragments arranged by size: 2162 1917 1384 1324 268 182 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7064 AflIII A'CryG_T Cuts at: 1203 4260 6160 7137 1203 Size: 3057 1900 977 1327 Fragments arranged by size: 3057 1900 1327 977 AgeI A'CCGG_T Cuts at: 6092 6092 Size: 7261 AhdI GACnn_n'nnGTC Cuts at: 5990 5990 Size: 7261 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4140 1184 Size: 2956 4305 AlwNI CAG_nnn'CTG Cuts at: 2731 3689 3872 4153 4207 5004 6576 2731 Size: 958 183 281 54 797 1572 3416 Fragments arranged by size: 3416 1572 958 797 281 183 54 ApaI G_GGCC'C Cuts at: 2226 3017 2226 Size: 791 6470 ApaLI G'TGCA_C Cuts at: 4376 5318 6474 4376 Size: 942 1156 5163 Fragments arranged by size: 5163 1156 942 ApoI r'AATT_y Cuts at: 1493 2326 3692 4570 4796 4948 7057 1493 Size: 833 1366 878 226 152 2109 1697 Fragments arranged by size: 2109 1697 1366 878 833 226 152 AseI AT'TA_AT Cuts at: 160 1032 5815 160 Size: 872 4783 1606 Fragments arranged by size: 4783 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 AvaI C'yCGr_G Cuts at: 1870 1943 2098 4498 4586 1870 Size: 73 155 2400 88 4545 Fragments arranged by size: 4545 2400 155 88 73 AvrII C'CTAG_G Cuts at: 3503 3503 Size: 7261 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3940 3973 4206 4239 3940 Size: 33 233 33 6962 Fragments arranged by size: 6962 233 33 33 BamHI G'GATC_C Cuts at: 4591 4958 4591 Size: 367 6894 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3022 3214 3259 4222 4541 Size: 325 217 627 1235 192 45 963 319 Cuts at: 4541 4582 6037 618 Size: 41 1455 1842 Fragments arranged by size: 1842 1455 1235 963 627 325 319 217 192 45 41 BanII G_rGCy'C Cuts at: 1391 2226 2426 2964 3017 3231 4029 4176 4580 Size: 835 200 538 53 214 798 147 404 Cuts at: 4580 1391 Size: 4072 Fragments arranged by size: 4072 835 798 538 404 214 200 147 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3150 4094 928 Size: 971 1251 944 4095 Fragments arranged by size: 4095 1251 971 944 BbvCI CC'TCA_GC Cuts at: 3854 3914 3854 Size: 60 7201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5452 5486 1903 Size: 34 3515 34 3678 Fragments arranged by size: 3678 3515 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3652 4496 5153 6363 3652 Size: 844 657 1210 4550 Fragments arranged by size: 4550 1210 844 657 BclI T'GATC_A Cuts at: 1548 1548 Size: 7261 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2197 2719 3764 Size: 107 122 71 631 605 525 522 1045 Cuts at: 3764 4470 4526 5872 136 Size: 706 56 1346 1525 Fragments arranged by size: 1525 1346 1045 706 631 605 525 522 122 107 71 56 BglII A'GATC_T Cuts at: 7256 7256 Size: 7261 BlpI GC'TnA_GC Cuts at: 3609 4678 3609 Size: 1069 6192 Bme1580I G_kGCm'C Cuts at: 1790 2226 3017 3027 4048 4380 4475 4544 5322 Size: 436 791 10 1021 332 95 69 778 Cuts at: 5322 6478 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1021 791 778 436 332 95 69 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4992 5950 442 Size: 416 53 83 399 24 3575 958 1753 Fragments arranged by size: 3575 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7261 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3823 3823 Size: 7261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2404 2519 2656 3091 3245 3647 3732 5921 Size: 1165 115 137 435 154 402 85 2189 Cuts at: 5921 1239 Size: 2579 Fragments arranged by size: 2579 2189 1165 435 402 154 137 115 85 Bpu10I CC'TnA_GC Cuts at: 2616 3532 3854 3914 4512 2616 Size: 916 322 60 598 5365 Fragments arranged by size: 5365 916 598 322 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2302 2324 4043 5386 6251 6549 2302 Size: 22 1719 1343 865 298 3014 Fragments arranged by size: 3014 1719 1343 865 298 22 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5924 882 Size: 617 4425 2219 Fragments arranged by size: 4425 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5052 493 Size: 711 3848 2702 Fragments arranged by size: 3848 2702 711 BsaBI GATnn'nnATC Cuts at: 7255 7255 Size: 7261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2864 5068 5450 6085 Size: 53 83 186 936 1331 2204 382 635 Cuts at: 6085 275 Size: 1451 Fragments arranged by size: 2204 1451 1331 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2475 3657 5694 6092 6366 6513 6924 2475 Size: 1182 2037 398 274 147 411 2812 Fragments arranged by size: 2812 2037 1182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2639 2669 3310 3340 3842 Size: 30 165 30 1236 30 641 30 502 Cuts at: 3842 3872 1178 Size: 30 4567 Fragments arranged by size: 4567 1236 641 502 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 2723 3644 4154 4184 1186 Size: 1534 3 921 510 30 4263 Fragments arranged by size: 4263 1534 921 510 30 3 BseYI C'CCAG_C Cuts at: 2060 2588 2692 2966 3392 3397 3814 3821 4003 Size: 528 104 274 426 5 417 7 182 Cuts at: 4003 4047 4052 4146 4468 6464 6960 2060 Size: 44 5 94 322 1996 496 2361 Fragments arranged by size: 2361 1996 528 496 426 417 322 274 182 104 94 44 7 5 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3685 4277 1163 Size: 573 1949 592 4147 Fragments arranged by size: 4147 1949 592 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4711 5472 6500 1540 Size: 275 186 2710 761 1028 2301 Fragments arranged by size: 2710 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4037 4176 4380 5322 5407 6478 4037 Size: 139 204 942 85 1071 4820 Fragments arranged by size: 4820 1071 942 204 139 85 BsiWI C'GTAC_G Cuts at: 4286 4286 Size: 7261 BsmI GAATG_Cn' Cuts at: 4772 4865 4772 Size: 93 7168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2426 2964 3017 3027 3231 4029 Size: 399 436 200 538 53 10 204 798 Cuts at: 4029 4037 4048 4176 4380 4475 4544 4580 5322 Size: 8 11 128 204 95 69 36 742 Cuts at: 5322 5407 6478 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 798 742 538 436 399 204 204 200 128 95 85 69 53 36 11 10 8 BspEI T'CCGG_A Cuts at: 6924 6924 Size: 7261 BspHI T'CATG_A Cuts at: 5150 6880 5150 Size: 1730 5531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2012 2032 2128 2622 4616 844 Size: 1168 20 96 494 1994 3489 Fragments arranged by size: 3489 1994 1168 494 96 20 BsrBI CCG'CTC Cuts at: 1394 1823 2133 3802 4433 5148 1394 Size: 429 310 1669 631 715 3507 Fragments arranged by size: 3507 1669 715 631 429 310 BsrDI GCAATG_nn' Cuts at: 65 1248 2657 2842 5756 5930 65 Size: 1183 1409 185 2914 174 1396 Fragments arranged by size: 2914 1409 1396 1183 185 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 3951 5905 6092 1700 Size: 110 199 1942 1954 187 2869 Fragments arranged by size: 2869 1954 1942 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7261 BssHII G'CGCG_C Cuts at: 1925 2013 1925 Size: 88 7173 BssSI C'ACGA_G Cuts at: 4028 5321 6333 4028 Size: 1293 1012 4956 Fragments arranged by size: 4956 1293 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4207 794 Size: 3413 3848 BstBI TT'CG_AA Cuts at: 6065 6065 Size: 7261 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7261 BstXI CCAn_nnnn'nTGG Cuts at: 3056 3056 Size: 7261 BstYI r'GATC_y Cuts at: 1972 2361 4591 4958 5343 5360 6812 6898 6910 Size: 389 2230 367 385 17 1452 86 12 Cuts at: 6910 7256 1972 Size: 346 1977 Fragments arranged by size: 2230 1977 1452 389 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2460 2817 2460 Size: 357 6904 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2048 2217 2490 2979 3255 Size: 552 939 27 17 169 273 489 276 Cuts at: 3255 3972 4365 513 Size: 717 393 3409 Fragments arranged by size: 3409 939 717 552 489 393 276 273 169 27 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6522 Fragments arranged by size: 6522 683 56 BtsI GCAGTG_nn' Cuts at: 2867 3007 3267 3543 3561 3835 4777 5571 5591 Size: 140 260 276 18 274 942 794 20 Cuts at: 5591 2867 Size: 4537 Fragments arranged by size: 4537 942 794 276 274 260 140 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7261 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7226 DraI TTT'AAA Cuts at: 4565 4565 Size: 7261 DraIII CAC_nnn'GTG Cuts at: 4043 4043 Size: 7261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3066 6268 809 Size: 507 1750 3202 1802 Fragments arranged by size: 3202 1802 1750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2636 Size: 54 1425 50 275 18 177 21 608 Cuts at: 2636 3039 3740 3756 4439 4708 5597 7226 8 Size: 403 701 16 683 269 889 1629 43 Fragments arranged by size: 1629 1425 889 701 683 608 403 275 269 177 54 50 43 21 18 16 EagI C'GGCC_G Cuts at: 1537 1812 4708 1537 Size: 275 2896 4090 Fragments arranged by size: 4090 2896 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 2414 2672 2777 2999 3279 3299 5191 Size: 923 138 258 105 222 280 20 1892 Cuts at: 5191 1353 Size: 3423 Fragments arranged by size: 3423 1892 923 280 258 222 138 105 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 2639 4376 5848 6222 6368 Size: 1064 138 765 285 1737 1472 374 146 Cuts at: 6368 387 Size: 1280 Fragments arranged by size: 1737 1472 1280 1064 765 374 285 146 138 EcoICRI GAG'CTC Cuts at: 4174 4174 Size: 7261 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2404 2519 2656 2956 2980 3091 3162 Size: 393 772 115 137 300 24 111 71 Cuts at: 3162 3245 3647 3732 5324 5921 6708 6976 1239 Size: 83 402 85 1592 597 787 268 1524 Fragments arranged by size: 1592 1524 787 772 597 402 393 300 268 137 115 111 85 83 71 24 EcoNI CCTnn'n_nnAGG Cuts at: 3333 3333 Size: 7261 EcoO109I rG'GnC_Cy Cuts at: 2044 2095 2222 2223 2388 2456 2698 3006 3669 Size: 51 127 1 165 68 242 308 663 Cuts at: 3669 3777 3850 3869 2044 Size: 108 73 19 5436 Fragments arranged by size: 5436 663 308 242 165 127 108 73 68 51 19 1 EcoRI G'AATT_C Cuts at: 3692 4570 4948 3692 Size: 878 378 6005 Fragments arranged by size: 6005 878 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4006 4006 Size: 7261 FspI TGC'GCA Cuts at: 2472 5767 2472 Size: 3295 3966 FspAI rTGC'GCAy Cuts at: 2472 2472 Size: 7261 HaeII r_GCGC'y Cuts at: 1678 3114 3821 3991 6408 1678 Size: 1436 707 170 2417 2531 Fragments arranged by size: 2531 2417 1436 707 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2072 2092 2104 2346 Size: 32 135 32 488 12 20 12 242 Cuts at: 2346 2378 3842 3874 5902 5934 5976 6008 1373 Size: 32 1464 32 2028 32 42 32 2626 Fragments arranged by size: 2626 2028 1464 488 242 135 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2986 4605 4851 669 Size: 988 281 1048 1619 246 3079 Fragments arranged by size: 3079 1619 1048 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7261 HpaI GTT'AAC Cuts at: 4851 4851 Size: 7261 KpnI G_GTAC'C Cuts at: 4226 4586 4226 Size: 360 6901 MfeI C'AATT_G Cuts at: 2740 4860 2740 Size: 2120 5141 MluI A'CGCG_T Cuts at: 7137 7137 Size: 7261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 2778 6375 6559 1305 Size: 948 525 3597 184 2007 Fragments arranged by size: 3597 2007 948 525 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5439 Fragments arranged by size: 5439 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 4106 5219 5578 5737 518 Size: 648 1362 1578 1113 359 159 2042 Fragments arranged by size: 2042 1578 1362 1113 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2050 2151 2809 3087 3349 3595 3825 Size: 427 17 101 658 278 262 246 230 Cuts at: 3825 3974 4094 4153 4207 4468 4677 5354 6502 Size: 149 120 59 54 261 209 677 1148 Cuts at: 6502 6747 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 658 427 278 262 261 246 245 230 209 149 120 101 59 54 17 NaeI GCC'GGC Cuts at: 2011 2011 Size: 7261 NcoI C'CATG_G Cuts at: 513 1065 2004 3255 513 Size: 552 939 1251 4519 Fragments arranged by size: 4519 1251 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7261 NgoMIV G'CCGG_C Cuts at: 2009 2009 Size: 7261 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7261 NotI GC'GGCC_GC Cuts at: 4708 4708 Size: 7261 NspI r_CATG'y Cuts at: 1132 1229 4264 4619 6164 1132 Size: 97 3035 355 1545 2229 Fragments arranged by size: 3035 2229 1545 355 97 PasI CC'CwG_GG Cuts at: 1348 3010 3665 3872 1348 Size: 1662 655 207 4737 Fragments arranged by size: 4737 1662 655 207 PciI A'CATG_T Cuts at: 4260 6160 4260 Size: 1900 5361 PflMI CCAn_nnn'nTGG Cuts at: 1108 3872 1108 Size: 2764 4497 PfoI T'CCnGG_A Cuts at: 2496 2730 2496 Size: 234 7027 PmeI GTTT'AAAC Cuts at: 4565 4565 Size: 7261 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5330 5362 6843 6875 5330 Size: 32 1481 32 5716 Fragments arranged by size: 5716 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2044 2095 2698 3006 3669 3850 2044 Size: 51 603 308 663 181 5455 Fragments arranged by size: 5455 663 603 308 181 51 PshAI GACnn'nnGTC Cuts at: 1613 2100 1613 Size: 487 6774 PsiI TTA'TAA Cuts at: 4831 4831 Size: 7261 PspOMI G'GGCC_C Cuts at: 2222 3013 2222 Size: 791 6470 PstI C_TGCA'G Cuts at: 830 2354 2454 2808 2874 3282 3364 4086 4613 Size: 1524 100 354 66 408 82 722 527 Cuts at: 4613 830 Size: 3478 Fragments arranged by size: 3478 1524 722 527 408 354 100 82 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7261 PvuII CAG'CTG Cuts at: 2809 3087 3595 4094 4153 4207 4468 2809 Size: 278 508 499 59 54 261 5602 Fragments arranged by size: 5602 508 499 278 261 59 54 SacI G_AGCT'C Cuts at: 4176 4176 Size: 7261 SacII CC_GC'GG Cuts at: 2034 2051 3975 2034 Size: 17 1924 5320 Fragments arranged by size: 5320 1924 17 SalI G'TCGA_C Cuts at: 1936 4603 1936 Size: 2667 4594 SanDI GG'GwC_CC Cuts at: 2698 3006 3669 2698 Size: 308 663 6290 Fragments arranged by size: 6290 663 308 SapI GCTCTTCn'nnn_ Cuts at: 2414 2672 3279 2414 Size: 258 607 6396 Fragments arranged by size: 6396 607 258 SbfI CC_TGCA'GG Cuts at: 2454 4613 2454 Size: 2159 5102 ScaI AGT'ACT Cuts at: 4418 5509 7221 4418 Size: 1091 1712 4458 Fragments arranged by size: 4458 1712 1091 SfcI C'TryA_G Cuts at: 826 1045 2293 2350 2450 2804 2870 3071 3278 Size: 219 1248 57 100 354 66 201 207 Cuts at: 3278 3360 4082 4112 4609 5744 6425 6616 826 Size: 82 722 30 497 1135 681 191 1471 Fragments arranged by size: 1471 1248 1135 722 681 497 354 219 207 201 191 100 82 66 57 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3764 3764 Size: 7261 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7261 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7261 SmaI CCC'GGG Cuts at: 1872 4500 4588 1872 Size: 2628 88 4545 Fragments arranged by size: 4545 2628 88 SmlI C'TyrA_G Cuts at: 820 1017 1943 2281 2339 4058 5365 6266 6528 Size: 197 926 338 58 1719 1307 901 262 Cuts at: 6528 820 Size: 1553 Fragments arranged by size: 1719 1553 1307 926 901 338 262 197 58 SnaBI TAC'GTA Cuts at: 493 5052 493 Size: 4559 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7261 SphI G_CATG'C Cuts at: 1132 4619 1132 Size: 3487 3774 SspI AAT'ATT Cuts at: 5 52 5185 5 Size: 47 5133 2081 Fragments arranged by size: 5133 2081 47 StuI AGG'CCT Cuts at: 1517 2337 3222 1517 Size: 820 885 5556 Fragments arranged by size: 5556 885 820 StyI C'CwwG_G Cuts at: 513 1065 2004 3099 3210 3255 3432 3503 4546 Size: 552 939 1095 111 45 177 71 1043 Cuts at: 4546 4700 513 Size: 154 3074 Fragments arranged by size: 3074 1095 1043 939 552 177 154 111 71 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3280 3388 3959 4300 4508 5288 5305 Size: 1255 1700 108 571 341 208 780 17 Cuts at: 5305 5458 5643 6088 325 Size: 153 185 445 1498 Fragments arranged by size: 1700 1498 1255 780 571 445 341 208 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4098 4416 5507 Size: 274 80 33 51 1443 2121 318 1091 Cuts at: 5507 7219 96 Size: 1712 138 Fragments arranged by size: 2121 1712 1443 1091 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2618 2639 2650 2671 3231 3263 3310 3342 3372 Size: 21 11 21 560 32 47 32 30 Cuts at: 3372 3404 3535 3567 3694 3726 7221 7253 2618 Size: 32 131 32 127 32 3495 32 2626 Fragments arranged by size: 3495 2626 560 131 127 47 32 32 32 32 32 32 30 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 2785 4274 1316 Size: 1469 1489 4303 Fragments arranged by size: 4303 1489 1469 XbaI T'CTAG_A Cuts at: 4597 4597 Size: 7261 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7261 XmaI C'CCGG_G Cuts at: 1870 4498 4586 1870 Size: 2628 88 4545 Fragments arranged by size: 4545 2628 88 XmnI GAAnn'nnTTC Cuts at: 2327 5390 2327 Size: 3063 4198 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5069 276 Size: 53 83 186 936 3535 2468 Fragments arranged by size: 3535 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BsmBI BstZ17I EcoRV FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfoI SrfI SwaI XcmI