Restriction map for
pFN21AB0430
(Linear) (Six-base) MAPSORT of: KIBB0430.seq Check: 3958 from: 1 to: 2556
KIBB0430.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 617 2556
Size: 617 1939
AccI GT'mk_AC
Cuts at: 0 1065 2556
Size: 1065 1491
Acc65I G'GTAC_C
Cuts at: 0 2217 2556
Size: 2217 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 975 1157 2556
Size: 951 24 182 1399
Fragments arranged by size:
1399 951 182 24
AflIII A'CryG_T
Cuts at: 0 2255 2556
Size: 2255 301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2135 2556
Size: 2135 421
AlwNI CAG_nnn'CTG
Cuts at: 0 726 1684 1867 2148 2202 2556
Size: 726 958 183 281 54 354
Fragments arranged by size:
958 726 354 281 183 54
ApaI G_GGCC'C
Cuts at: 0 221 1012 2556
Size: 221 791 1544
Fragments arranged by size:
1544 791 221
ApaLI G'TGCA_C
Cuts at: 0 2371 2556
Size: 2371 185
ApoI r'AATT_y
Cuts at: 0 321 1687 2556
Size: 321 1366 869
Fragments arranged by size:
1366 869 321
AvaI C'yCGr_G
Cuts at: 0 93 2493 2556
Size: 93 2400 63
Fragments arranged by size:
2400 93 63
AvrII C'CTAG_G
Cuts at: 0 1498 2556
Size: 1498 1058
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1935 1968 2201 2234 2556
Size: 1935 33 233 33 322
Fragments arranged by size:
1935 322 233 33 33
BanI G'GyrC_C
Cuts at: 0 1017 1209 1254 2217 2536 2556
Size: 1017 192 45 963 319 20
Fragments arranged by size:
1017 963 319 192 45 20
BanII G_rGCy'C
Cuts at: 0 221 421 959 1012 1226 2024 2171 2556
Size: 221 200 538 53 214 798 147 385
Fragments arranged by size:
798 538 385 221 214 200 147 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 2089 2556
Size: 1145 944 467
Fragments arranged by size:
1145 944 467
BbvCI CC'TCA_GC
Cuts at: 0 1849 1909 2556
Size: 1849 60 647
Fragments arranged by size:
1849 647 60
BciVI GTATCCnnnnn_n'
Cuts at: 0 1647 2491 2556
Size: 1647 844 65
Fragments arranged by size:
1647 844 65
BglI GCCn_nnn'nGGC
Cuts at: 0 192 714 1759 2465 2521 2556
Size: 192 522 1045 706 56 35
Fragments arranged by size:
1045 706 522 192 56 35
BlpI GC'TnA_GC
Cuts at: 0 1604 2556
Size: 1604 952
Bme1580I G_kGCm'C
Cuts at: 0 221 1012 1022 2043 2375 2470 2539 2556
Size: 221 791 10 1021 332 95 69 17
Fragments arranged by size:
1021 791 332 221 95 69 17 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1818 2556
Size: 1818 738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 514 651 1086 1240 1642 1727 2556
Size: 399 115 137 435 154 402 85 829
Fragments arranged by size:
829 435 402 399 154 137 115 85
Bpu10I CC'TnA_GC
Cuts at: 0 611 1527 1849 1909 2507 2556
Size: 611 916 322 60 598 49
Fragments arranged by size:
916 611 598 322 60 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 319 2038 2556
Size: 297 22 1719 518
Fragments arranged by size:
1719 518 297 22
BsaHI Gr'CG_yC
Cuts at: 0 859 2556
Size: 859 1697
BsaWI w'CCGG_w
Cuts at: 0 470 1652 2556
Size: 470 1182 904
Fragments arranged by size:
1182 904 470
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 1305 1335 1837 1867 2556
Size: 634 30 641 30 502 30 689
Fragments arranged by size:
689 641 634 502 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 715 718 1639 2149 2179 2556
Size: 715 3 921 510 30 377
Fragments arranged by size:
921 715 510 377 30 3
BseYI C'CCAG_C
Cuts at: 0 55 583 687 961 1387 1392 1809 1816
Size: 55 528 104 274 426 5 417 7
Cuts at: 1816 1998 2042 2047 2141 2463 2556
Size: 182 44 5 94 322 93
Fragments arranged by size:
528 426 417 322 274 182 104 94
93 55 44 7 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1680 2272 2556
Size: 1680 592 284
Fragments arranged by size:
1680 592 284
BsiHKAI G_wGCw'C
Cuts at: 0 2032 2171 2375 2556
Size: 2032 139 204 181
Fragments arranged by size:
2032 204 181 139
BsiWI C'GTAC_G
Cuts at: 0 2281 2556
Size: 2281 275
Bsp1286I G_dGCh'C
Cuts at: 0 221 421 959 1012 1022 1226 2024 2032
Size: 221 200 538 53 10 204 798 8
Cuts at: 2032 2043 2171 2375 2470 2539 2556
Size: 11 128 204 95 69 17
Fragments arranged by size:
798 538 221 204 204 200 128 95
69 53 17 11 10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 7 27 123 617 2556
Size: 7 20 96 494 1939
Fragments arranged by size:
1939 494 96 20 7
BsrBI CCG'CTC
Cuts at: 0 128 1797 2428 2556
Size: 128 1669 631 128
Fragments arranged by size:
1669 631 128 128
BsrDI GCAATG_nn'
Cuts at: 0 652 837 2556
Size: 652 185 1719
Fragments arranged by size:
1719 652 185
BsrFI r'CCGG_y
Cuts at: 0 4 1946 2556
Size: 4 1942 610
Fragments arranged by size:
1942 610 4
BssHII G'CGCG_C
Cuts at: 0 8 2556
Size: 8 2548
BssSI C'ACGA_G
Cuts at: 0 2023 2556
Size: 2023 533
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2202 2556
Size: 2202 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1051 2556
Size: 1051 1505
BstYI r'GATC_y
Cuts at: 0 356 2556
Size: 356 2200
Bsu36I CC'TnA_GG
Cuts at: 0 455 812 2556
Size: 455 357 1744
Fragments arranged by size:
1744 455 357
BtgI C'CryG_G
Cuts at: 0 26 43 212 485 974 1250 1967 2360
Size: 26 17 169 273 489 276 717 393
Cuts at: 2360 2556
Size: 196
Fragments arranged by size:
717 489 393 276 273 196 169 26
17
BtsI GCAGTG_nn'
Cuts at: 0 862 1002 1262 1538 1556 1830 2556
Size: 862 140 260 276 18 274 726
Fragments arranged by size:
862 726 276 274 260 140 18
DraIII CAC_nnn'GTG
Cuts at: 0 2038 2556
Size: 2038 518
DrdI GACnn_nn'nnGTC
Cuts at: 0 1061 2556
Size: 1061 1495
EaeI y'GGCC_r
Cuts at: 0 2 23 631 1034 1735 1751 2434 2556
Size: 2 21 608 403 701 16 683 122
Fragments arranged by size:
701 683 608 403 122 21 16 2
EarI CTCTTCn'nnn_
Cuts at: 0 271 409 667 772 994 1274 1294 2556
Size: 271 138 258 105 222 280 20 1262
Fragments arranged by size:
1262 280 271 258 222 138 105 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 349 634 2371 2556
Size: 349 285 1737 185
Fragments arranged by size:
1737 349 285 185
EcoICRI GAG'CTC
Cuts at: 0 2169 2556
Size: 2169 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 514 651 951 975 1086 1157 1240
Size: 399 115 137 300 24 111 71 83
Cuts at: 1240 1642 1727 2556
Size: 402 85 829
Fragments arranged by size:
829 402 399 300 137 115 111 85
83 71 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1328 2556
Size: 1328 1228
EcoO109I rG'GnC_Cy
Cuts at: 0 39 90 217 218 383 451 693 1001
Size: 39 51 127 1 165 68 242 308
Cuts at: 1001 1664 1772 1845 1864 2556
Size: 663 108 73 19 692
Fragments arranged by size:
692 663 308 242 165 127 108 73
68 51 39 19 1
EcoRI G'AATT_C
Cuts at: 0 1687 2556
Size: 1687 869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2001 2556
Size: 2001 555
FspI TGC'GCA
Cuts at: 0 467 2556
Size: 467 2089
FspAI rTGC'GCAy
Cuts at: 0 467 2556
Size: 467 2089
HaeII r_GCGC'y
Cuts at: 0 1109 1816 1986 2556
Size: 1109 707 170 570
Fragments arranged by size:
1109 707 570 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 67 87 99 341 373 1837 1869
Size: 55 12 20 12 242 32 1464 32
Cuts at: 1869 2556
Size: 687
Fragments arranged by size:
1464 687 242 55 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 981 2556
Size: 981 1575
KpnI G_GTAC'C
Cuts at: 0 2221 2556
Size: 2221 335
MfeI C'AATT_G
Cuts at: 0 735 2556
Size: 735 1821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 773 2556
Size: 248 525 1783
Fragments arranged by size:
1783 525 248
MslI CAynn'nnrTG
Cuts at: 0 523 2101 2556
Size: 523 1578 455
Fragments arranged by size:
1578 523 455
MspA1I CmG'CkG
Cuts at: 0 28 45 146 804 1082 1344 1590 1820
Size: 28 17 101 658 278 262 246 230
Cuts at: 1820 1969 2089 2148 2202 2463 2556
Size: 149 120 59 54 261 93
Fragments arranged by size:
658 278 262 261 246 230 149 120
101 93 59 54 28 17
NaeI GCC'GGC
Cuts at: 0 6 2556
Size: 6 2550
NcoI C'CATG_G
Cuts at: 0 1250 2556
Size: 1250 1306
NgoMIV G'CCGG_C
Cuts at: 0 4 2556
Size: 4 2552
NspI r_CATG'y
Cuts at: 0 2259 2556
Size: 2259 297
PasI CC'CwG_GG
Cuts at: 0 1005 1660 1867 2556
Size: 1005 655 207 689
Fragments arranged by size:
1005 689 655 207
PciI A'CATG_T
Cuts at: 0 2255 2556
Size: 2255 301
PflMI CCAn_nnn'nTGG
Cuts at: 0 1867 2556
Size: 1867 689
PfoI T'CCnGG_A
Cuts at: 0 491 725 2556
Size: 491 234 1831
Fragments arranged by size:
1831 491 234
PpuMI rG'GwC_Cy
Cuts at: 0 39 90 693 1001 1664 1845 2556
Size: 39 51 603 308 663 181 711
Fragments arranged by size:
711 663 603 308 181 51 39
PshAI GACnn'nnGTC
Cuts at: 0 95 2556
Size: 95 2461
PspOMI G'GGCC_C
Cuts at: 0 217 1008 2556
Size: 217 791 1548
Fragments arranged by size:
1548 791 217
PstI C_TGCA'G
Cuts at: 0 349 449 803 869 1277 1359 2081 2556
Size: 349 100 354 66 408 82 722 475
Fragments arranged by size:
722 475 408 354 349 100 82 66
PvuII CAG'CTG
Cuts at: 0 804 1082 1590 2089 2148 2202 2463 2556
Size: 804 278 508 499 59 54 261 93
Fragments arranged by size:
804 508 499 278 261 93 59 54
SacI G_AGCT'C
Cuts at: 0 2171 2556
Size: 2171 385
SacII CC_GC'GG
Cuts at: 0 29 46 1970 2556
Size: 29 17 1924 586
Fragments arranged by size:
1924 586 29 17
SanDI GG'GwC_CC
Cuts at: 0 693 1001 1664 2556
Size: 693 308 663 892
Fragments arranged by size:
892 693 663 308
SapI GCTCTTCn'nnn_
Cuts at: 0 409 667 1274 2556
Size: 409 258 607 1282
Fragments arranged by size:
1282 607 409 258
SbfI CC_TGCA'GG
Cuts at: 0 449 2556
Size: 449 2107
ScaI AGT'ACT
Cuts at: 0 2413 2556
Size: 2413 143
SfcI C'TryA_G
Cuts at: 0 288 345 445 799 865 1066 1273 1355
Size: 288 57 100 354 66 201 207 82
Cuts at: 1355 2077 2107 2556
Size: 722 30 449
Fragments arranged by size:
722 449 354 288 207 201 100 82
66 57 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1759 2556
Size: 1759 797
SmaI CCC'GGG
Cuts at: 0 2495 2556
Size: 2495 61
SmlI C'TyrA_G
Cuts at: 0 276 334 2053 2556
Size: 276 58 1719 503
Fragments arranged by size:
1719 503 276 58
StuI AGG'CCT
Cuts at: 0 332 1217 2556
Size: 332 885 1339
Fragments arranged by size:
1339 885 332
StyI C'CwwG_G
Cuts at: 0 1094 1205 1250 1427 1498 2541 2556
Size: 1094 111 45 177 71 1043 15
Fragments arranged by size:
1094 1043 177 111 71 45 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1275 1383 1954 2295 2503 2556
Size: 1275 108 571 341 208 53
Fragments arranged by size:
1275 571 341 208 108 53
TatI w'GTAC_w
Cuts at: 0 2093 2411 2556
Size: 2093 318 145
Fragments arranged by size:
2093 318 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 634 645 666 1226 1258 1305 1337
Size: 613 21 11 21 560 32 47 32
Cuts at: 1337 1367 1399 1530 1562 1689 1721 2556
Size: 30 32 131 32 127 32 835
Fragments arranged by size:
835 613 560 131 127 47 32 32
32 32 32 30 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 0 780 2269 2556
Size: 780 1489 287
Fragments arranged by size:
1489 780 287
XmaI C'CCGG_G
Cuts at: 0 2493 2556
Size: 2493 63
XmnI GAAnn'nnTTC
Cuts at: 0 322 2556
Size: 322 2234
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BanI BanII BbsI
BbvCI BciVI BglI BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KpnI MfeI
MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SanDI SapI SbfI ScaI SfcI SfiI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI BamHI BbeI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI BsaI BsaAI BsaBI BsiEI
BsmI BsmBI BspEI BspHI BsrGI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EagI EcoRV FseI HindIII
HpaI KasI MluI MscI NarI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI
XhoI ZraI