Restriction map for
pFN21AB6677
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccI AccIII AciI AclI
AcyI AflIII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoT22I FauI FseI FspI
Hin1I HindIII HpaI KasI KspI
MaeI MaeII MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 678 GGTACC G^GTACC
AccII 348 CGCG CG^CG
AflII 892 CTTAAG C^TTAAG
Alw44I 807 GTGCAC G^TGCAC
AocI 15 CCTCAGG CC^T[ACGT]AGG
ApaLI 807 GTGCAC G^TGCAC
Asp718I 678 GGTACC G^GTACC
BalI 647 TGGCCA TGG^CCA
BamHI 629 GGATCC G^GATCC
BanI 678 GGTACC G^G[CT][GA]CC
BanII 130 GAGCTC G[GA]GC[CT]^C
BclI 444 TGATCA T^GATCA
BfrI 892 CTTAAG C^TTAAG
BlpI 132 GCTCAGC GC^T[ACGT]AGC
BpmI 567 CTGGAG CTGGAG(16/14)
Bpu1102I 132 GCTCAGC GC^T[ACGT]AGC
BsaI 374 GAGACC GGTCTC(1/5)
BsaWI 1253 ACCGGA [AT]^CCGG[AT]
BsmFI 1192 GTCCC GGGAC(10/14)
BsmI 291 GAATGC GAATGC(1/-1)
BspHI 830 TCATGA T^CATGA
BsrDI 182 GCAATG GCAATG(2/0)
BstEII 144 GGTTACC G^GT[ACGT]ACC
BstPI 144 GGTTACC G^GT[ACGT]ACC
BstUI 348 CGCG CG^CG
BstXI 161 CCACTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 629 GGATCC [GA]^GATC[CT]
Bsu36I 15 CCTCAGG CC^T[ACGT]AGG
CelII 132 GCTCAGC GC^T[ACGT]AGC
DraI 1126 TTTAAA TTT^AAA
EaeI 647 TGGCCA [CT]^GGCC[GA]
Eco81I 15 CCTCAGG CC^T[ACGT]AGG
EcoO65I 144 GGTTACC G^GT[ACGT]ACC
EcoRI 1080 GAATTC G^AATTC
EcoRV 255 GATATC GAT^ATC
FbaI 444 TGATCA T^GATCA
HaeII 431 GGCGCT [GA]GCGC^[CT]
HapII 1254 CCGG C^CGG
HgaI 900 GCGTC GACGC(5/10)
HincII 541 GTTGAC GT[CT]^[GA]AC
HindII 541 GTTGAC GT[CT]^[GA]AC
HpaII 1254 CCGG C^CGG
KpnI 678 GGTACC GGTAC^C
MflI 629 GGATCC [GA]^GATC[CT]
MluNI 647 TGGCCA TGG^CCA
MscI 647 TGGCCA TGG^CCA
MspI 1254 CCGG C^CGG
MvnI 348 CGCG CG^CG
PleI 570 GAGTC GAGTC(4/5)
PshAI 1185 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 830 TCATGA T^CATGA
SacI 130 GAGCTC GAGCT^C
SapI 774 GCTCTTC GCTCTTC(1/4)
TaqI 903 TCGA T^CGA
TfiI 591 GATTC G^A[AT]TC
TthHB8I 903 TCGA T^CGA
XhoII 629 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AspHI 130 GAGCTC G[AT]GC[AT]^C
807 GTGCAC
AvaII 171 GGTCC G^G[AT]CC
1191 GGTCC
BmyI 130 GAGCTC G[AGT]GC[ACT]^C
807 GTGCAC
BsiHKAI 130 GAGCTC G[AT]GC[AT]^C
807 GTGCAC
BsmAI 374 GAGAC GTCTC(1/5)
619 GTCTC
Bsp1286I 130 GAGCTC G[AGT]GC[ACT]^C
807 GTGCAC
EarI 775 CTCTTC CTCTTC(1/4)
962 CTCTTC
EcoT14I 369 CCTTGG C^C[AT][AT]GG
800 CCTTGG
HinfI 570 GAGTC G^A[ACGT]TC
591 GATTC
Ksp632I 775 CTCTTC CTCTTC(1/4)
962 CTCTTC
MslI 97 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
825 CACCCTCATG
SfaNI 22 GCATC GCATC(5/9)
1211 GCATC
SmlI 867 CTTGAG C^T[CT][GA]AG
892 CTTAAG
StyI 369 CCTTGG C^C[AT][AT]GG
800 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp509I TspRI