(Circular) (Six-base) MAPSORT of: pFN21AB6653.seq Check: 9197 from: 1 to: 5932 pFN21AB6653 5932 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 2189 Size: 5932 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3742 278 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2433 3275 1937 Size: 496 842 4594 Fragments arranged by size: 4594 842 496 Acc65I G'GTAC_C Cuts at: 3253 3253 Size: 5932 AclI AA'CG_TT Cuts at: 1563 4059 4432 1563 Size: 2496 373 3063 Fragments arranged by size: 3063 2496 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2538 2722 3212 3995 5379 5647 1632 Size: 539 367 184 490 783 1384 268 1917 Fragments arranged by size: 1917 1384 783 539 490 367 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5735 AflIII A'CryG_T Cuts at: 1203 4831 5808 1203 Size: 3628 977 1327 Fragments arranged by size: 3628 1327 977 AgeI A'CCGG_T Cuts at: 2589 4763 2589 Size: 2174 3758 AhdI GACnn_n'nnGTC Cuts at: 4661 4661 Size: 5932 AleI CACnn'nnGTG Cuts at: 1166 2921 1166 Size: 1755 4177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5932 AlwNI CAG_nnn'CTG Cuts at: 3675 5247 3675 Size: 1572 4360 ApaLI G'TGCA_C Cuts at: 3989 5145 3989 Size: 1156 4776 ApoI r'AATT_y Cuts at: 1493 3128 3241 3467 3619 5728 1493 Size: 1635 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1635 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 4486 160 Size: 872 3454 1606 Fragments arranged by size: 3454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2603 2997 3257 1870 Size: 73 75 585 394 260 4545 Fragments arranged by size: 4545 585 394 260 75 73 BamHI G'GATC_C Cuts at: 3262 3629 3262 Size: 367 5565 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2275 2933 3253 4708 618 Size: 325 217 627 488 658 320 1455 1842 Fragments arranged by size: 1842 1455 658 627 488 325 320 217 BanII G_rGCy'C Cuts at: 1391 2048 2646 3251 1391 Size: 657 598 605 4072 Fragments arranged by size: 4072 657 605 598 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 2211 928 Size: 971 124 188 4649 Fragments arranged by size: 4649 971 188 124 BbvCI CC'TCA_GC Cuts at: 2321 2321 Size: 5932 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4123 4157 1903 Size: 34 2186 34 3678 Fragments arranged by size: 3678 2186 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2404 3824 5034 2404 Size: 1420 1210 3302 Fragments arranged by size: 3302 1420 1210 BclI T'GATC_A Cuts at: 1548 3220 1548 Size: 1672 4260 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4543 136 Size: 107 122 71 631 605 2871 1525 Fragments arranged by size: 2871 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5927 5927 Size: 5932 BlpI GC'TnA_GC Cuts at: 2840 3349 2840 Size: 509 5423 Bme1580I G_kGCm'C Cuts at: 1790 2280 3993 5149 1790 Size: 490 1713 1156 2573 Fragments arranged by size: 2573 1713 1156 490 BmgBI CAC'GTC Cuts at: 1318 2665 1318 Size: 1347 4585 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2743 3073 3663 Size: 416 53 83 399 24 1326 330 590 Cuts at: 3663 4621 442 Size: 958 1753 Fragments arranged by size: 1753 1326 958 590 416 399 330 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5932 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4592 1239 Size: 3353 2579 Bpu10I CC'TnA_GC Cuts at: 2321 2321 Size: 5932 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 4057 4922 5220 2870 Size: 1187 865 298 3582 Fragments arranged by size: 3582 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2870 4595 882 Size: 617 1371 1725 2219 Fragments arranged by size: 2219 1725 1371 617 BsaAI yAC'GTr Cuts at: 493 1204 3723 493 Size: 711 2519 2702 Fragments arranged by size: 2702 2519 711 BsaBI GATnn'nnATC Cuts at: 5926 5926 Size: 5932 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3739 4121 4756 275 Size: 53 83 186 936 2206 382 635 1451 Fragments arranged by size: 2206 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2589 3091 4365 4763 5037 5184 5595 2589 Size: 502 1274 398 274 147 411 2926 Fragments arranged by size: 2926 1274 502 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5707 Fragments arranged by size: 5707 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5932 BseYI C'CCAG_C Cuts at: 2218 2329 2547 2798 2950 5135 5631 2218 Size: 111 218 251 152 2185 496 2519 Fragments arranged by size: 2519 2185 496 251 218 152 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2540 2582 1163 Size: 573 804 42 4513 Fragments arranged by size: 4513 804 573 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3382 4143 5171 1540 Size: 275 186 1381 761 1028 2301 Fragments arranged by size: 2301 1381 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3993 4078 5149 3993 Size: 85 1071 4776 Fragments arranged by size: 4776 1071 85 BsmI GAATG_Cn' Cuts at: 3443 3536 3443 Size: 93 5839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2280 2646 3251 3993 4078 5149 Size: 399 258 232 366 605 742 85 1071 Cuts at: 5149 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 399 366 258 232 85 BspEI T'CCGG_A Cuts at: 3091 5595 3091 Size: 2504 3428 BspHI T'CATG_A Cuts at: 3821 5551 3821 Size: 1730 4202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 3287 844 Size: 1345 1098 3489 Fragments arranged by size: 3489 1345 1098 BsrBI CCG'CTC Cuts at: 1394 1823 3089 3819 1394 Size: 429 1266 730 3507 Fragments arranged by size: 3507 1266 730 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4427 4601 65 Size: 1183 3179 174 1396 Fragments arranged by size: 3179 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2313 2589 4576 4763 1700 Size: 110 503 276 1987 187 2869 Fragments arranged by size: 2869 1987 503 276 187 110 BsrGI T'GTAC_A Cuts at: 96 2820 96 Size: 2724 3208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5932 BssSI C'ACGA_G Cuts at: 2891 3992 5004 2891 Size: 1101 1012 3819 Fragments arranged by size: 3819 1101 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5932 BstBI TT'CG_AA Cuts at: 4736 4736 Size: 5932 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5932 BstYI r'GATC_y Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927 Size: 1290 367 385 17 1452 86 12 346 Cuts at: 5927 1972 Size: 1977 Fragments arranged by size: 1977 1452 1290 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2635 513 Size: 552 939 631 3810 Fragments arranged by size: 3810 939 631 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2233 487 Size: 683 56 1007 4186 Fragments arranged by size: 4186 1007 683 56 BtsI GCAGTG_nn' Cuts at: 2390 2834 3448 4242 4262 2390 Size: 444 614 794 20 4060 Fragments arranged by size: 4060 794 614 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5932 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5897 DraI TTT'AAA Cuts at: 3236 3236 Size: 5932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4939 809 Size: 507 3623 1802 Fragments arranged by size: 3623 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2197 3379 4268 Size: 54 1425 50 275 18 367 1182 889 Cuts at: 4268 5897 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1182 889 367 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3379 1537 Size: 275 1567 4090 Fragments arranged by size: 4090 1567 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 2741 3862 1353 Size: 1113 275 1121 3423 Fragments arranged by size: 3423 1121 1113 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4519 4893 5039 387 Size: 1064 138 2930 374 146 1280 Fragments arranged by size: 2930 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2538 2722 3212 3995 4592 5379 Size: 393 539 367 184 490 783 597 787 Cuts at: 5379 5647 1239 Size: 268 1524 Fragments arranged by size: 1524 787 783 597 539 490 393 367 268 184 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2618 Size: 5932 EcoRI G'AATT_C Cuts at: 3241 3619 3241 Size: 378 5554 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 5932 FspI TGC'GCA Cuts at: 4438 4438 Size: 5932 HaeII r_GCGC'y Cuts at: 1678 5079 1678 Size: 3401 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2777 2809 4573 4605 4647 Size: 32 135 32 1205 32 1764 32 42 Cuts at: 4647 4679 1373 Size: 32 2626 Fragments arranged by size: 2626 1764 1205 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3276 3522 669 Size: 988 281 1338 246 3079 Fragments arranged by size: 3079 1338 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5932 HpaI GTT'AAC Cuts at: 3522 3522 Size: 5932 KpnI G_GTAC'C Cuts at: 3257 3257 Size: 5932 MfeI C'AATT_G Cuts at: 3531 3531 Size: 5932 MluI A'CGCG_T Cuts at: 5808 5808 Size: 5932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5046 5230 1305 Size: 3741 184 2007 Fragments arranged by size: 3741 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4110 Fragments arranged by size: 4110 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3890 4249 4408 518 Size: 648 1755 969 359 159 2042 Fragments arranged by size: 2042 1755 969 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3348 4025 5173 5418 1606 Size: 1742 677 1148 245 2120 Fragments arranged by size: 2120 1742 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2635 513 Size: 552 939 631 3810 Fragments arranged by size: 3810 939 631 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5932 NotI GC'GGCC_GC Cuts at: 3379 3379 Size: 5932 NspI r_CATG'y Cuts at: 1132 1229 2342 3290 4835 1132 Size: 97 1113 948 1545 2229 Fragments arranged by size: 2229 1545 1113 948 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5932 PciI A'CATG_T Cuts at: 4831 4831 Size: 5932 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5932 PmeI GTTT'AAAC Cuts at: 3236 3236 Size: 5932 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4001 4033 5514 5546 4001 Size: 32 1481 32 4387 Fragments arranged by size: 4387 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2027 3079 3101 1613 Size: 414 1052 22 4444 Fragments arranged by size: 4444 1052 414 22 PsiI TTA'TAA Cuts at: 3502 3502 Size: 5932 PspXI vC'TCGA_Gb Cuts at: 2603 2603 Size: 5932 PstI C_TGCA'G Cuts at: 830 2310 2397 3284 830 Size: 1480 87 887 3478 Fragments arranged by size: 3478 1480 887 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5932 SalI G'TCGA_C Cuts at: 1936 3274 1936 Size: 1338 4594 SbfI CC_TGCA'GG Cuts at: 2310 3284 2310 Size: 974 4958 ScaI AGT'ACT Cuts at: 4180 5892 4180 Size: 1712 4220 SexAI A'CCwGG_T Cuts at: 2787 2787 Size: 5932 SfcI C'TryA_G Cuts at: 826 1045 2115 2306 2393 2968 3280 4415 5096 Size: 219 1070 191 87 575 312 1135 681 Cuts at: 5096 5287 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1070 681 575 312 219 191 191 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5932 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5932 SmaI CCC'GGG Cuts at: 1872 2020 3259 1872 Size: 148 1239 4545 Fragments arranged by size: 4545 1239 148 SmlI C'TyrA_G Cuts at: 820 1017 1943 2603 2885 2997 4036 4937 5199 Size: 197 926 660 282 112 1039 901 262 Cuts at: 5199 820 Size: 1553 Fragments arranged by size: 1553 1039 926 901 660 282 262 197 112 SnaBI TAC'GTA Cuts at: 493 3723 493 Size: 3230 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5932 SphI G_CATG'C Cuts at: 1132 2342 3290 1132 Size: 1210 948 3774 Fragments arranged by size: 3774 1210 948 SspI AAT'ATT Cuts at: 5 52 3856 5 Size: 47 3804 2081 Fragments arranged by size: 3804 2081 47 StuI AGG'CCT Cuts at: 1517 2264 2423 1517 Size: 747 159 5026 Fragments arranged by size: 5026 747 159 StyI C'CwwG_G Cuts at: 513 1065 2004 2635 3371 513 Size: 552 939 631 736 3074 Fragments arranged by size: 3074 939 736 631 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3959 3976 4129 4314 4759 325 Size: 1255 2379 17 153 185 445 1498 Fragments arranged by size: 2379 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2628 2751 Size: 274 80 33 51 1443 90 561 123 Cuts at: 2751 2820 4178 5890 96 Size: 69 1358 1712 138 Fragments arranged by size: 1712 1443 1358 561 274 138 123 90 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 2946 2978 5892 5924 2559 Size: 32 355 32 2914 32 2567 Fragments arranged by size: 2914 2567 355 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5932 XbaI T'CTAG_A Cuts at: 3268 3268 Size: 5932 XhoI C'TCGA_G Cuts at: 1943 2603 2997 1943 Size: 660 394 4878 Fragments arranged by size: 4878 660 394 XmaI C'CCGG_G Cuts at: 1870 2018 3257 1870 Size: 148 1239 4545 Fragments arranged by size: 4545 1239 148 XmnI GAAnn'nnTTC Cuts at: 4061 4061 Size: 5932 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3740 276 Size: 53 83 186 936 2206 2468 Fragments arranged by size: 2468 2206 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI