Restriction map for
pFN21AB6653
(Linear) (Six-base) MAPSORT of: KIBB6653.seq Check: 846 from: 1 to: 1227
KIBB6653.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 1227
Size: 184 1043
AccI GT'mk_AC
Cuts at: 0 428 1227
Size: 428 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 533 717 1207 1227
Size: 166 367 184 490 20
Fragments arranged by size:
490 367 184 166 20
AgeI A'CCGG_T
Cuts at: 0 584 1227
Size: 584 643
AleI CACnn'nnGTG
Cuts at: 0 916 1227
Size: 916 311
ApoI r'AATT_y
Cuts at: 0 1123 1227
Size: 1123 104
AvaI C'yCGr_G
Cuts at: 0 13 598 992 1227
Size: 13 585 394 235
Fragments arranged by size:
585 394 235 13
BanI G'GyrC_C
Cuts at: 0 270 928 1227
Size: 270 658 299
Fragments arranged by size:
658 299 270
BanII G_rGCy'C
Cuts at: 0 43 641 1227
Size: 43 598 586
Fragments arranged by size:
598 586 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 206 1227
Size: 18 188 1021
Fragments arranged by size:
1021 188 18
BbvCI CC'TCA_GC
Cuts at: 0 316 1227
Size: 316 911
BciVI GTATCCnnnnn_n'
Cuts at: 0 399 1227
Size: 399 828
BclI T'GATC_A
Cuts at: 0 1215 1227
Size: 1215 12
BlpI GC'TnA_GC
Cuts at: 0 835 1227
Size: 835 392
Bme1580I G_kGCm'C
Cuts at: 0 275 1227
Size: 275 952
BmgBI CAC'GTC
Cuts at: 0 660 1227
Size: 660 567
BmrI ACTGGGnnnn_n'
Cuts at: 0 738 1068 1227
Size: 738 330 159
Fragments arranged by size:
738 330 159
Bpu10I CC'TnA_GC
Cuts at: 0 316 1227
Size: 316 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1227
Size: 865 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 865 1227
Size: 865 362
BsaWI w'CCGG_w
Cuts at: 0 584 1086 1227
Size: 584 502 141
Fragments arranged by size:
584 502 141
BseYI C'CCAG_C
Cuts at: 0 213 324 542 793 945 1227
Size: 213 111 218 251 152 282
Fragments arranged by size:
282 251 218 213 152 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 577 1227
Size: 535 42 650
Fragments arranged by size:
650 535 42
Bsp1286I G_dGCh'C
Cuts at: 0 43 275 641 1227
Size: 43 232 366 586
Fragments arranged by size:
586 366 232 43
BspEI T'CCGG_A
Cuts at: 0 1086 1227
Size: 1086 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 1227
Size: 184 1043
BsrBI CCG'CTC
Cuts at: 0 1084 1227
Size: 1084 143
BsrFI r'CCGG_y
Cuts at: 0 308 584 1227
Size: 308 276 643
Fragments arranged by size:
643 308 276
BsrGI T'GTAC_A
Cuts at: 0 815 1227
Size: 815 412
BssSI C'ACGA_G
Cuts at: 0 886 1227
Size: 886 341
BtgI C'CryG_G
Cuts at: 0 630 1227
Size: 630 597
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 228 1227
Size: 228 999
BtsI GCAGTG_nn'
Cuts at: 0 385 829 1227
Size: 385 444 398
Fragments arranged by size:
444 398 385
EaeI y'GGCC_r
Cuts at: 0 192 1227
Size: 192 1035
EarI CTCTTCn'nnn_
Cuts at: 0 461 736 1227
Size: 461 275 491
Fragments arranged by size:
491 461 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 533 717 1207 1227
Size: 166 367 184 490 20
Fragments arranged by size:
490 367 184 166 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1227
Size: 613 614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 1227
Size: 96 1131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1227
Size: 772 32 423
Fragments arranged by size:
772 423 32
MslI CAynn'nnrTG
Cuts at: 0 916 1227
Size: 916 311
NcoI C'CATG_G
Cuts at: 0 630 1227
Size: 630 597
NspI r_CATG'y
Cuts at: 0 337 1227
Size: 337 890
PshAI GACnn'nnGTC
Cuts at: 0 22 1074 1096 1227
Size: 22 1052 22 131
Fragments arranged by size:
1052 131 22 22
PspXI vC'TCGA_Gb
Cuts at: 0 598 1227
Size: 598 629
PstI C_TGCA'G
Cuts at: 0 305 392 1227
Size: 305 87 835
Fragments arranged by size:
835 305 87
SbfI CC_TGCA'GG
Cuts at: 0 305 1227
Size: 305 922
SexAI A'CCwGG_T
Cuts at: 0 782 1227
Size: 782 445
SfcI C'TryA_G
Cuts at: 0 110 301 388 963 1227
Size: 110 191 87 575 264
Fragments arranged by size:
575 264 191 110 87
SmaI CCC'GGG
Cuts at: 0 15 1227
Size: 15 1212
SmlI C'TyrA_G
Cuts at: 0 598 880 992 1227
Size: 598 282 112 235
Fragments arranged by size:
598 282 235 112
SphI G_CATG'C
Cuts at: 0 337 1227
Size: 337 890
StuI AGG'CCT
Cuts at: 0 259 418 1227
Size: 259 159 809
Fragments arranged by size:
809 259 159
StyI C'CwwG_G
Cuts at: 0 630 1227
Size: 630 597
TatI w'GTAC_w
Cuts at: 0 62 623 746 815 1227
Size: 62 561 123 69 412
Fragments arranged by size:
561 412 123 69 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 941 973 1227
Size: 554 32 355 32 254
Fragments arranged by size:
554 355 254 32 32
XhoI C'TCGA_G
Cuts at: 0 598 992 1227
Size: 598 394 235
Fragments arranged by size:
598 394 235
XmaI C'CCGG_G
Cuts at: 0 13 1227
Size: 13 1214
Enzymes that do cut:
AarI AccI AcuI AgeI AleI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BclI BlpI Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaI BsaWI BseYI BsgI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BssSI BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoNI FalI Hin4I MslI
NcoI NspI PshAI PspXI PstI SbfI SexAI SfcI
SmaI SmlI SphI StuI StyI TatI TstI XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BfrBI BglI BglII BmtI
BplI BpmI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI
XcmI XmnI ZraI