(Circular) (Six-base) MAPSORT of: pFN21AB6629.seq Check: 4167 from: 1 to: 5950 pFN21AB6629 5950 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 2028 3271 2028 Size: 1243 4707 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 3215 4013 5397 5665 1632 Size: 1040 543 798 1384 268 1917 Fragments arranged by size: 1917 1384 1040 798 543 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2862 4849 5826 1203 Size: 1659 1987 977 1327 Fragments arranged by size: 1987 1659 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 2593 4679 2593 Size: 2086 3864 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 2650 3693 5265 2650 Size: 1043 1572 3335 Fragments arranged by size: 3335 1572 1043 ApaI G_GGCC'C Cuts at: 3136 3136 Size: 5950 ApaLI G'TGCA_C Cuts at: 3242 4007 5163 3242 Size: 765 1156 4029 Fragments arranged by size: 4029 1156 765 ApoI r'AATT_y Cuts at: 1493 2300 2744 2761 2885 3259 3485 3637 5746 Size: 807 444 17 124 374 226 152 2109 Cuts at: 5746 1493 Size: 1697 Fragments arranged by size: 2109 1697 807 444 374 226 152 124 17 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2891 3271 4726 618 Size: 325 217 627 241 863 380 1455 1842 Fragments arranged by size: 1842 1455 863 627 380 325 241 217 BanII G_rGCy'C Cuts at: 1391 2633 3136 3269 1391 Size: 1242 503 133 4072 Fragments arranged by size: 4072 1242 503 133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3221 928 Size: 971 1322 3657 Fragments arranged by size: 3657 1322 971 BbvCI CC'TCA_GC Cuts at: 2145 2145 Size: 5950 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 2340 1548 Size: 792 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2900 4561 136 Size: 107 122 71 631 605 1228 1661 1525 Fragments arranged by size: 1661 1525 1228 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 3136 3246 4011 5167 1790 Size: 1346 110 765 1156 2573 Fragments arranged by size: 2573 1346 1156 765 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3681 4639 442 Size: 416 53 83 399 24 2264 958 1753 Fragments arranged by size: 2264 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2278 2291 4610 1239 Size: 1039 13 2319 2579 Fragments arranged by size: 2579 2319 1039 13 Bpu10I CC'TnA_GC Cuts at: 2145 2145 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2608 2863 3741 493 Size: 711 1404 255 878 2702 Fragments arranged by size: 2702 1404 878 711 255 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2913 3757 4139 4774 Size: 53 83 186 936 1380 844 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1451 1380 936 844 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2823 4383 4781 5055 5202 5613 2823 Size: 1560 398 274 147 411 3160 Fragments arranged by size: 3160 1560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2276 2306 1178 Size: 30 165 30 688 30 155 30 4822 Fragments arranged by size: 4822 688 165 155 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2202 2954 3139 1186 Size: 1016 752 185 3997 Fragments arranged by size: 3997 1016 752 185 BseYI C'CCAG_C Cuts at: 2035 2257 5153 5649 2035 Size: 222 2896 496 2336 Fragments arranged by size: 2896 2336 496 222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3107 1163 Size: 573 1371 4006 Fragments arranged by size: 4006 1371 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3400 4161 5189 1540 Size: 275 186 1000 399 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 399 275 186 BsiHKAI G_wGCw'C Cuts at: 3246 4011 4096 5167 3246 Size: 765 85 1071 4029 Fragments arranged by size: 4029 1071 765 85 BsmI GAATG_Cn' Cuts at: 3461 3554 3461 Size: 93 5857 BsmBI CGTCTCn'nnnn_ Cuts at: 3088 3088 Size: 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2633 3136 3246 3269 4011 4096 5167 Size: 399 843 503 110 23 742 85 1071 Cuts at: 5167 1391 Size: 2174 Fragments arranged by size: 2174 1071 843 742 503 399 110 85 23 BspEI T'CCGG_A Cuts at: 2823 5613 2823 Size: 2790 3160 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2817 3305 844 Size: 1973 488 3489 Fragments arranged by size: 3489 1973 488 BsrBI CCG'CTC Cuts at: 1394 1823 2920 2944 3103 3837 1394 Size: 429 1097 24 159 734 3507 Fragments arranged by size: 3507 1097 734 429 159 24 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 4445 4619 65 Size: 1183 840 2357 174 1396 Fragments arranged by size: 2357 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 4594 4781 1700 Size: 110 221 2563 187 2869 Fragments arranged by size: 2869 2563 221 187 110 BsrGI T'GTAC_A Cuts at: 96 2439 96 Size: 2343 3607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2974 3127 513 Size: 552 939 970 153 3336 Fragments arranged by size: 3336 970 939 552 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2822 487 Size: 683 56 1596 3615 Fragments arranged by size: 3615 1596 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2237 4957 809 Size: 507 921 2720 1802 Fragments arranged by size: 2720 1802 921 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2460 2998 3397 Size: 54 1425 50 275 18 630 538 399 Cuts at: 3397 4286 5915 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 630 538 399 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 3397 1537 Size: 275 1186 399 4090 Fragments arranged by size: 4090 1186 399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2285 2575 3880 1353 Size: 744 188 290 1305 3423 Fragments arranged by size: 3423 1305 744 290 188 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2278 2291 2672 3215 4013 4610 5397 Size: 393 646 13 381 543 798 597 787 Cuts at: 5397 5665 1239 Size: 268 1524 Fragments arranged by size: 1524 798 787 646 597 543 393 381 268 13 EcoO109I rG'GnC_Cy Cuts at: 2275 3132 3133 2275 Size: 857 1 5092 Fragments arranged by size: 5092 857 1 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2579 2611 3193 Size: 32 135 32 609 32 366 32 582 Cuts at: 3193 3225 4591 4623 4665 4697 1373 Size: 32 1366 32 42 32 2626 Fragments arranged by size: 2626 1366 609 582 366 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3142 3294 3540 669 Size: 988 281 1204 152 246 3079 Fragments arranged by size: 3079 1204 988 281 246 152 HindIII A'AGCT_T Cuts at: 748 2117 748 Size: 1369 4581 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 2032 3275 2032 Size: 1243 4707 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3180 5064 5248 1305 Size: 1875 1884 184 2007 Fragments arranged by size: 2007 1884 1875 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 3498 Fragments arranged by size: 3498 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2385 2717 3908 4267 4426 518 Size: 648 1080 139 332 1191 359 159 2042 Fragments arranged by size: 2042 1191 1080 648 359 332 159 139 MspA1I CmG'CkG Cuts at: 1606 2055 2976 3129 3366 4043 5191 5436 1606 Size: 449 921 153 237 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 449 245 237 153 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4459 Fragments arranged by size: 4459 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2881 3190 3308 4853 1132 Size: 97 1652 309 118 1545 2229 Fragments arranged by size: 2229 1652 1545 309 118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 2211 1108 Size: 1103 4847 PfoI T'CCnGG_A Cuts at: 2294 2294 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2275 Size: 5950 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 3132 3132 Size: 5950 PstI C_TGCA'G Cuts at: 830 2098 3302 830 Size: 1268 1204 3478 Fragments arranged by size: 3478 1268 1204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SacII CC_GC'GG Cuts at: 2977 3130 2977 Size: 153 5797 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SexAI A'CCwGG_T Cuts at: 2433 2433 Size: 5950 SfcI C'TryA_G Cuts at: 826 1045 2094 3298 4433 5114 5305 826 Size: 219 1049 1204 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 1049 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2560 3389 513 Size: 552 939 556 829 3074 Fragments arranged by size: 3074 939 829 556 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 2949 3977 3994 4147 4332 4777 Size: 1255 921 448 1028 17 153 185 445 Cuts at: 4777 325 Size: 1498 Fragments arranged by size: 1498 1255 1028 921 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2616 4196 Size: 274 80 33 51 1443 462 177 1580 Cuts at: 4196 5908 96 Size: 1712 138 Fragments arranged by size: 1712 1580 1443 462 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2358 2831 4079 2358 Size: 473 1248 4229 Fragments arranged by size: 4229 1248 473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI