Restriction map for pFN21AB6629
 (Linear) (Six-base) MAPSORT of: KIBB6629.seq  Check: 6000  from: 1  to: 1245



KIBB6629.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

Acc65I G'GTAC_C

Cuts at:      0      23    1245 
   Size:         23    1222
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1210    1245 
   Size:        667     543      35

  Fragments arranged by size: 

                 667     543      35
 
AflIII A'CryG_T

Cuts at:      0     857    1245 
   Size:        857     388
 
AhdI GACnn_n'nnGTC

Cuts at:      0     588    1245 
   Size:        588     657
 
AlwNI CAG_nnn'CTG

Cuts at:      0     645    1245 
   Size:        645     600
 
ApaI G_GGCC'C

Cuts at:      0    1131    1245 
   Size:       1131     114
 
ApaLI G'TGCA_C

Cuts at:      0    1237    1245 
   Size:       1237       8
 
ApoI r'AATT_y

Cuts at:      0     295     739     756     880    1245 
   Size:        295     444      17     124     365

  Fragments arranged by size: 

                 444     365     295     124      17
 
BanI G'GyrC_C

Cuts at:      0      23     886    1245 
   Size:         23     863     359

  Fragments arranged by size: 

                 863     359      23
 
BanII G_rGCy'C

Cuts at:      0     628    1131    1245 
   Size:        628     503     114

  Fragments arranged by size: 

                 628     503     114
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1216    1245 
   Size:       1216      29
 
BbvCI CC'TCA_GC

Cuts at:      0     140    1245 
   Size:        140    1105
 
BclI T'GATC_A

Cuts at:      0     335    1245 
   Size:        335     910
 
BglI GCCn_nnn'nGGC

Cuts at:      0     895    1245 
   Size:        895     350
 
Bme1580I G_kGCm'C

Cuts at:      0    1131    1241    1245 
   Size:       1131     110       4

  Fragments arranged by size: 

                1131     110       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     273     286    1245 
   Size:        273      13     959

  Fragments arranged by size: 

                 959     273      13
 
Bpu10I CC'TnA_GC

Cuts at:      0     140    1245 
   Size:        140    1105
 
BsaAI yAC'GTr

Cuts at:      0     603     858    1245 
   Size:        603     255     387

  Fragments arranged by size: 

                 603     387     255
 
BsaHI Gr'CG_yC

Cuts at:      0     908    1245 
   Size:        908     337
 
BsaWI w'CCGG_w

Cuts at:      0     818    1245 
   Size:        818     427
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      86     116     271     301    1245 
   Size:         86      30     155      30     944

  Fragments arranged by size: 

                 944     155      86      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     197     949    1134    1245 
   Size:        197     752     185     111

  Fragments arranged by size: 

                 752     197     185     111
 
BseYI C'CCAG_C

Cuts at:      0      30     252    1245 
   Size:         30     222     993

  Fragments arranged by size: 

                 993     222      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1102    1245 
   Size:       1102     143
 
BsiEI CG_ry'CG

Cuts at:      0     996    1245 
   Size:        996     249
 
BsiHKAI G_wGCw'C

Cuts at:      0    1241    1245 
   Size:       1241       4
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1083    1245 
   Size:       1083     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     628    1131    1241    1245 
   Size:        628     503     110       4

  Fragments arranged by size: 

                 628     503     110       4
 
BspEI T'CCGG_A

Cuts at:      0     818    1245 
   Size:        818     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     812    1245 
   Size:        812     433
 
BsrBI CCG'CTC

Cuts at:      0     915     939    1098    1245 
   Size:        915      24     159     147

  Fragments arranged by size: 

                 915     159     147      24
 
BsrDI GCAATG_nn'

Cuts at:      0      83    1245 
   Size:         83    1162
 
BsrFI r'CCGG_y

Cuts at:      0      26    1245 
   Size:         26    1219
 
BsrGI T'GTAC_A

Cuts at:      0     434    1245 
   Size:        434     811
 
BtgI C'CryG_G

Cuts at:      0     969    1122    1245 
   Size:        969     153     123

  Fragments arranged by size: 

                 969     153     123
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     817    1245 
   Size:        817     428
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     232    1245 
   Size:        232    1013
 
EaeI y'GGCC_r

Cuts at:      0     455     993    1245 
   Size:        455     538     252

  Fragments arranged by size: 

                 538     455     252
 
EagI C'GGCC_G

Cuts at:      0     993    1245 
   Size:        993     252
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     280     570    1245 
   Size:         92     188     290     675

  Fragments arranged by size: 

                 675     290     188      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     273     286     667    1210    1245 
   Size:        273      13     381     543      35

  Fragments arranged by size: 

                 543     381     273      35      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270    1127    1128    1245 
   Size:        270     857       1     117

  Fragments arranged by size: 

                 857     270     117       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     176     208     574     606    1188    1220    1245 
   Size:        176      32     366      32     582      32      25

  Fragments arranged by size: 

                 582     366     176      32      32      32      25
 
HincII GTy'rAC

Cuts at:      0    1137    1245 
   Size:       1137     108
 
HindIII A'AGCT_T

Cuts at:      0     112    1245 
   Size:        112    1133
 
KpnI G_GTAC'C

Cuts at:      0      27    1245 
   Size:         27    1218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1175    1245 
   Size:       1175      70
 
MscI TGG'CCA

Cuts at:      0     457    1245 
   Size:        457     788
 
MslI CAynn'nnrTG

Cuts at:      0     241     380     712    1245 
   Size:        241     139     332     533

  Fragments arranged by size: 

                 533     332     241     139
 
MspA1I CmG'CkG

Cuts at:      0      50     971    1124    1245 
   Size:         50     921     153     121

  Fragments arranged by size: 

                 921     153     121      50
 
NspI r_CATG'y

Cuts at:      0     876    1185    1245 
   Size:        876     309      60

  Fragments arranged by size: 

                 876     309      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     206    1245 
   Size:        206    1039
 
PfoI T'CCnGG_A

Cuts at:      0     289    1245 
   Size:        289     956
 
PpuMI rG'GwC_Cy

Cuts at:      0     270    1245 
   Size:        270     975
 
PspOMI G'GGCC_C

Cuts at:      0    1127    1245 
   Size:       1127     118
 
PstI C_TGCA'G

Cuts at:      0      93    1245 
   Size:         93    1152
 
SacII CC_GC'GG

Cuts at:      0     972    1125    1245 
   Size:        972     153     120

  Fragments arranged by size: 

                 972     153     120
 
SexAI A'CCwGG_T

Cuts at:      0     428    1245 
   Size:        428     817
 
SfcI C'TryA_G

Cuts at:      0      89    1245 
   Size:         89    1156
 
StyI C'CwwG_G

Cuts at:      0     555    1245 
   Size:        555     690
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     496     944    1245 
   Size:        496     448     301

  Fragments arranged by size: 

                 496     448     301
 
TatI w'GTAC_w

Cuts at:      0     434     611    1245 
   Size:        434     177     634

  Fragments arranged by size: 

                 634     434     177
 
XmnI GAAnn'nnTTC

Cuts at:      0     353     826    1245 
   Size:        353     473     419

  Fragments arranged by size: 

                 473     419     353
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI    AlwNI     ApaI    ApaLI     ApoI
     BanI    BanII     BbsI    BbvCI     BclI     BglI Bme1580I     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI     BtgI    BtgZI     DrdI     EaeI     EagI     EarI
  Eco57MI EcoO109I    Hin4I   HincII  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     NspI    PflMI     PfoI    PpuMI   PspOMI     PstI
    SacII    SexAI     SfcI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsiWI     BsmI
    BspHI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     MfeI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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