Restriction map for pFN21AB6553
 (Linear) (Six-base) MAPSORT of: KIBB6553.seq  Check: 6931  from: 1  to: 1113



KIBB6553.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AflIII A'CryG_T

Cuts at:      0     222     782    1113 
   Size:        222     560     331

  Fragments arranged by size: 

                 560     331     222
 
AlwNI CAG_nnn'CTG

Cuts at:      0     793    1113 
   Size:        793     320
 
AvaI C'yCGr_G

Cuts at:      0      92     110    1028    1113 
   Size:         92      18     918      85

  Fragments arranged by size: 

                 918      92      85      18
 
BanI G'GyrC_C

Cuts at:      0     213     645     708     737     852     897     912    1113 
   Size:        213     432      63      29     115      45      15     201

  Fragments arranged by size: 

                 432     213     201     115      63      45      29      15
            
 
BanII G_rGCy'C

Cuts at:      0     462     596     780    1113 
   Size:        462     134     184     333

  Fragments arranged by size: 

                 462     333     184     134
 
BbeI G_GCGC'C

Cuts at:      0     741     916    1113 
   Size:        741     175     197

  Fragments arranged by size: 

                 741     197     175
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     219    1113 
   Size:        219     894
 
BfrBI ATG'CAT

Cuts at:      0     976    1113 
   Size:        976     137
 
BglI GCCn_nnn'nGGC

Cuts at:      0     203     256     298     735     758    1113 
   Size:        203      53      42     437      23     355

  Fragments arranged by size: 

                 437     355     203      53      42      23
 
Bme1580I G_kGCm'C

Cuts at:      0     216     855    1029    1113 
   Size:        216     639     174      84

  Fragments arranged by size: 

                 639     216     174      84
 
BmgBI CAC'GTC

Cuts at:      0     225    1113 
   Size:        225     888
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     219    1113 
   Size:        219     894
 
BmtI G_CTAG'C

Cuts at:      0     109    1113 
   Size:        109    1004
 
Bpu10I CC'TnA_GC

Cuts at:      0     157    1113 
   Size:        157     956
 
BsaAI yAC'GTr

Cuts at:      0     783    1113 
   Size:        783     330
 
BsaHI Gr'CG_yC

Cuts at:      0      10     738     750     913    1113 
   Size:         10     728      12     163     200

  Fragments arranged by size: 

                 728     200     163      12      10
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     767     797    1113 
   Size:        767      30     316

  Fragments arranged by size: 

                 767     316      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     366    1077    1113 
   Size:        366     711      36

  Fragments arranged by size: 

                 711     366      36
 
BseYI C'CCAG_C

Cuts at:      0      49      58     284     518     536     575     848    1045 
   Size:         49       9     226     234      18      39     273     197

Cuts at:   1045    1113 
   Size:         68

  Fragments arranged by size: 

                 273     234     226     197      68      49      39      18
                   9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889     939    1056    1113 
   Size:        889      50     117      57

  Fragments arranged by size: 

                 889     117      57      50
 
BsiEI CG_ry'CG

Cuts at:      0     309     475    1113 
   Size:        309     166     638

  Fragments arranged by size: 

                 638     309     166
 
BsiHKAI G_wGCw'C

Cuts at:      0     680    1113 
   Size:        680     433
 
Bsp1286I G_dGCh'C

Cuts at:      0     216     462     596     680     780     855    1029    1113 
   Size:        216     246     134      84     100      75     174      84

  Fragments arranged by size: 

                 246     216     174     134     100      84      84      75
            
 
BsrDI GCAATG_nn'

Cuts at:      0     168    1113 
   Size:        168     945
 
BsrFI r'CCGG_y

Cuts at:      0     197     470     474    1113 
   Size:        197     273       4     639

  Fragments arranged by size: 

                 639     273     197       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     402     454    1113 
   Size:        402      52     659

  Fragments arranged by size: 

                 659     402      52
 
BstYI r'GATC_y

Cuts at:      0     968    1113 
   Size:        968     145
 
BtgI C'CryG_G

Cuts at:      0     260     315     447    1113 
   Size:        260      55     132     666

  Fragments arranged by size: 

                 666     260     132      55
 
EaeI y'GGCC_r

Cuts at:      0     234     248     257     392     472     797     938    1094 
   Size:        234      14       9     135      80     325     141     156

Cuts at:   1094    1113 
   Size:         19

  Fragments arranged by size: 

                 325     234     156     141     135      80      19      14
                   9
 
EagI C'GGCC_G

Cuts at:      0     472    1113 
   Size:        472     641
 
EarI CTCTTCn'nnn_

Cuts at:      0     529    1113 
   Size:        529     584
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     130    1113 
   Size:        130     983
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     905    1096    1113 
   Size:        905     191      17

  Fragments arranged by size: 

                 905     191      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      96     888    1054    1113 
   Size:         96     792     166      59

  Fragments arranged by size: 

                 792     166      96      59
 
HaeII r_GCGC'y

Cuts at:      0     127     741     916    1113 
   Size:        127     614     175     197

  Fragments arranged by size: 

                 614     197     175     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1093    1113 
   Size:       1093      20
 
HindIII A'AGCT_T

Cuts at:      0    1011    1113 
   Size:       1011     102
 
KasI G'GCGC_C

Cuts at:      0     737     912    1113 
   Size:        737     175     201

  Fragments arranged by size: 

                 737     201     175
 
MscI TGG'CCA

Cuts at:      0     236     394     799     940    1096    1113 
   Size:        236     158     405     141     156      17

  Fragments arranged by size: 

                 405     236     158     156     141      17
 
MslI CAynn'nnrTG

Cuts at:      0     452    1113 
   Size:        452     661
 
MspA1I CmG'CkG

Cuts at:      0      25     102     288     560     793     873     953    1113 
   Size:         25      77     186     272     233      80      80     160

  Fragments arranged by size: 

                 272     233     186     160      80      80      77      25
            
 
NaeI GCC'GGC

Cuts at:      0     199    1113 
   Size:        199     914
 
NarI GG'CG_CC

Cuts at:      0     738     913    1113 
   Size:        738     175     200

  Fragments arranged by size: 

                 738     200     175
 
NcoI C'CATG_G

Cuts at:      0     447    1113 
   Size:        447     666
 
NgoMIV G'CCGG_C

Cuts at:      0     197    1113 
   Size:        197     916
 
NheI G'CTAG_C

Cuts at:      0     105    1113 
   Size:        105    1008
 
NsiI A_TGCA'T

Cuts at:      0     978    1113 
   Size:        978     135
 
NspI r_CATG'y

Cuts at:      0     369    1113 
   Size:        369     744
 
PasI CC'CwG_GG

Cuts at:      0     596     654    1113 
   Size:        596      58     459

  Fragments arranged by size: 

                 596     459      58
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     965    1113 
   Size:        965     148
 
PfoI T'CCnGG_A

Cuts at:      0     686    1113 
   Size:        686     427
 
PmlI CAC'GTG

Cuts at:      0     783    1113 
   Size:        783     330
 
PpuMI rG'GwC_Cy

Cuts at:      0      96     888    1054    1113 
   Size:         96     792     166      59

  Fragments arranged by size: 

                 792     166      96      59
 
PstI C_TGCA'G

Cuts at:      0     356    1113 
   Size:        356     757
 
PvuII CAG'CTG

Cuts at:      0     288     793     873     953    1113 
   Size:        288     505      80      80     160

  Fragments arranged by size: 

                 505     288     160      80      80
 
SanDI GG'GwC_CC

Cuts at:      0      96     888    1054    1113 
   Size:         96     792     166      59

  Fragments arranged by size: 

                 792     166      96      59
 
SapI GCTCTTCn'nnn_

Cuts at:      0     529    1113 
   Size:        529     584
 
SfcI C'TryA_G

Cuts at:      0     352    1113 
   Size:        352     761
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     203     256     298    1113 
   Size:        203      53      42     815

  Fragments arranged by size: 

                 815     203      53      42
 
SfoI GGC'GCC

Cuts at:      0     739     914    1113 
   Size:        739     175     199

  Fragments arranged by size: 

                 739     199     175
 
SmaI CCC'GGG

Cuts at:      0      94     112    1030    1113 
   Size:         94      18     918      83

  Fragments arranged by size: 

                 918      94      83      18
 
SrfI GCCC'GGGC

Cuts at:      0     112    1113 
   Size:        112    1001
 
StuI AGG'CCT

Cuts at:      0     729    1113 
   Size:        729     384
 
StyI C'CwwG_G

Cuts at:      0     447    1097    1113 
   Size:        447     650      16

  Fragments arranged by size: 

                 650     447      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     231    1059    1113 
   Size:        231     828      54

  Fragments arranged by size: 

                 828     231      54
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     767     799     845     877    1113 
   Size:        767      32      46      32     236

  Fragments arranged by size: 

                 767     236      46      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141    1089    1113 
   Size:        141     948      24

  Fragments arranged by size: 

                 948     141      24
 
XmaI C'CCGG_G

Cuts at:      0      92     110    1028    1113 
   Size:         92      18     918      85

  Fragments arranged by size: 

                 918      92      85      18
 

 Enzymes that do cut:

   AflIII    AlwNI     AvaI     BanI    BanII     BbeI     BbsI    BfrBI
     BglI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrDI
    BsrFI    BstXI    BstYI     BtgI     EaeI     EagI     EarI     EciI
    EcoNI EcoO109I    HaeII    Hin4I  HindIII     KasI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI     PstI    PvuII    SanDI
     SapI     SfcI     SfiI     SfoI     SmaI     SrfI     StuI     StyI
    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI     BclI    BglII     BlpI     BplI     BpmI    BpuEI     BsaI
    BsaBI    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII     HpaI     KpnI     MfeI     MluI     MmeI     NdeI     NotI
     NruI     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SbfI
     ScaI    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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